FastQCFastQC Report
Fri 27 May 2016
SRR939943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523185
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4500.01984.1233108-109
CCGCGCC4400.01972.8497108-109
CGTCGCT353.931491E-61766.5011110-111
CGTCGCG250.00534198571236.5507110-111
CCGCGCG1650.01202.4989108-109
CCGCTCG1450.01197.3158108-109
CGCGTCT1300.01188.9911110-111
CCGCGCT755.630318E-8992.0616108-109
CCGCGAC250.008302244992.0615108-109
CATCGCG807.287417E-8930.05774108-109
CCGCGCA552.956118E-5901.87415108-109
CCGTTCG552.956118E-5901.87415108-109
CGTCGTC1058.933057E-8883.25055110-111
CGGGTCG400.006864142772.8442110-111
CCGTGCG1052.1608685E-7708.6154108-109
CGTCTCG1402.8207432E-7662.43787110-111
CGTCGCC1507.3305273E-10661.3744108-109
CGTGTCG957.850483E-5650.81616110-111
CCGTTGC1153.108289E-7646.9967108-109
CGCCGCC3050.0608.13965110-111
Produced by FastQC (version 0.11.5)