##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939943_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523185 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21914212653092 33.0 31.0 34.0 28.0 34.0 2 31.318499722620693 33.0 31.0 34.0 28.0 34.0 3 31.454361092053823 34.0 31.0 34.0 28.0 34.0 4 34.77257325932175 37.0 35.0 37.0 32.0 37.0 5 34.76296575924789 37.0 35.0 37.0 32.0 37.0 6 34.928092122755935 37.0 35.0 37.0 32.0 37.0 7 34.81678784914505 37.0 35.0 37.0 32.0 37.0 8 34.83774787698146 37.0 35.0 37.0 32.0 37.0 9 36.48126918266659 39.0 37.0 39.0 32.0 39.0 10-11 36.45605064388108 39.0 37.0 39.0 32.0 39.0 12-13 36.40094571572068 39.0 37.0 39.0 32.0 39.0 14-15 37.81765149998195 41.0 38.5 41.0 32.5 41.0 16-17 37.774657050850685 41.0 38.0 41.0 32.0 41.0 18-19 37.749314758220436 41.0 38.5 41.0 32.0 41.0 20-21 37.79586097552169 41.0 39.0 41.0 32.0 41.0 22-23 37.73268742798807 40.0 38.0 41.0 32.0 41.0 24-25 37.737921854535074 40.5 38.0 41.0 32.0 41.0 26-27 37.69937729166188 40.0 38.0 41.0 32.0 41.0 28-29 37.632522969960974 40.0 38.0 41.0 32.0 41.0 30-31 37.55114316383105 40.0 38.0 41.0 32.0 41.0 32-33 37.45975899184931 40.0 38.0 41.0 31.5 41.0 34-35 37.38076957165413 40.0 38.0 41.0 31.0 41.0 36-37 37.55154987739506 40.0 38.0 41.0 32.0 41.0 38-39 37.5182072433749 40.0 38.0 41.0 32.0 41.0 40-41 37.45339666553964 40.0 38.0 41.0 31.0 41.0 42-43 37.35123048086739 40.0 38.0 41.0 31.0 41.0 44-45 37.226023431165615 40.0 38.0 41.0 31.0 41.0 46-47 37.12712835276083 40.0 38.0 41.0 31.0 41.0 48-49 37.026571624589266 40.0 38.0 41.0 30.0 41.0 50-51 36.90012572340195 40.0 37.0 41.0 30.0 41.0 52-53 36.77747154810479 40.0 37.0 41.0 30.0 41.0 54-55 36.624577447913424 40.0 37.0 41.0 30.0 41.0 56-57 36.44239504722013 40.0 36.0 41.0 30.0 41.0 58-59 36.27134753821762 40.0 36.0 41.0 29.5 41.0 60-61 36.04993122962739 39.5 35.5 41.0 29.0 41.0 62-63 35.79295948949077 39.0 35.0 41.0 29.0 41.0 64-65 35.50447220790646 39.0 35.0 41.0 28.0 41.0 66-67 35.21563467339817 38.5 35.0 40.5 28.0 41.0 68-69 34.85193459757022 37.5 35.0 40.0 28.0 41.0 70-71 34.46931200084035 37.0 34.0 40.0 27.5 41.0 72-73 34.07907214159803 36.5 34.0 39.0 26.5 41.0 74-75 33.66156638884968 36.0 34.0 39.0 26.0 41.0 76-77 33.218331653738716 35.5 34.0 38.5 26.0 40.0 78-79 32.78351644744401 35.0 34.0 37.0 25.0 39.0 80-81 32.36857637122214 35.0 33.0 37.0 24.5 39.0 82-83 32.180907112399346 35.0 34.0 36.5 24.5 39.0 84-85 31.766448592915502 35.0 34.0 36.0 20.0 37.0 86-87 31.080672078572203 35.0 34.0 36.0 2.0 37.0 88-89 15.807129797102782 18.0 16.5 18.5 2.0 19.0 90-91 2.7230402085104566 2.0 2.0 2.0 2.0 2.0 92-93 2.2706811713613253 2.0 2.0 2.0 2.0 2.0 94-95 2.091928754550498 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17392608831269385 1101 2 0.2023343005842939 1101 3 0.2649818857220865 1101 4 0.26595088724560156 1101 5 0.30887345908556796 1101 6 0.3093103234254855 1101 7 0.222344674095595 1101 8 0.2836500519015388 1101 9 0.3870442284232496 1101 10-11 0.21455350194556644 1101 12-13 0.3338238770151065 1101 14-15 0.31944255056042437 1101 16-17 0.3553322906199199 1101 18-19 0.27412862649329384 1101 20-21 0.3142024648602657 1101 22-23 0.27563264354746764 1101 24-25 0.30150858129184854 1101 26-27 0.240044533106321 1101 28-29 0.394239051244611 1101 30-31 0.22574976198070118 1101 32-33 0.3016062719153467 1101 34-35 0.32857492904521735 1101 36-37 0.33719132007441743 1101 38-39 0.2483824603789344 1101 40-41 0.17974975190279707 1101 42-43 0.27624671067749773 1101 44-45 0.2322904773685508 1101 46-47 0.3149764407871416 1101 48-49 0.3029961938140673 1101 50-51 0.3588707824819579 1101 52-53 0.2638319137276497 1101 54-55 0.16844166956735762 1101 56-57 0.21883368816645543 1101 58-59 0.10439974701340304 1101 60-61 0.16435667499938234 1101 62-63 0.1965998143427612 1101 64-65 0.15234093280030692 1101 66-67 0.13066918170480335 1101 68-69 0.19787860880735053 1101 70-71 0.13093516731628085 1101 72-73 0.14173361866098588 1101 74-75 0.02392651773188703 1101 76-77 0.01355397429524885 1101 78-79 0.12472524276648755 1101 80-81 0.13539143198615733 1101 82-83 0.13819843366100315 1101 84-85 0.1713094405646487 1101 86-87 0.12315884694052315 1101 88-89 0.7247818915893589 1101 90-91 -0.07678246834528135 1101 92-93 -0.20979438056155697 1101 94-95 -0.04052202292474005 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.05426397149442863 1102 2 0.0765321298941295 1102 3 0.1176377135807094 1102 4 0.17071427237606684 1102 5 0.12865174943559765 1102 6 0.10119727939285639 1102 7 0.11587862697604123 1102 8 0.15737953765400192 1102 9 0.19699991938318107 1102 10-11 0.2634117145942696 1102 12-13 0.2828386322025267 1102 14-15 0.28326588299041333 1102 16-17 0.24658489900518532 1102 18-19 0.17556415919410284 1102 20-21 0.2076420281541118 1102 22-23 0.27351034212659897 1102 24-25 0.19345187815565623 1102 26-27 0.23563514271661745 1102 28-29 0.31259532530240364 1102 30-31 0.29835292540601444 1102 32-33 0.22174997837139188 1102 34-35 0.2209055836516427 1102 36-37 0.2282937836876826 1102 38-39 0.3091132610526799 1102 40-41 0.23181552094477098 1102 42-43 0.12381180851714646 1102 44-45 0.16065103387513346 1102 46-47 0.24001365033360145 1102 48-49 0.2962252615716068 1102 50-51 0.24590154064659941 1102 52-53 0.213495794437236 1102 54-55 0.23296303439121857 1102 56-57 0.14065342928668656 1102 58-59 0.09227485269482116 1102 60-61 0.06398359959516142 1102 62-63 0.20893038334527603 1102 64-65 0.1698353330648743 1102 66-67 0.20040213576771748 1102 68-69 0.1625293707160438 1102 70-71 0.16604786825740803 1102 72-73 0.21063182185721274 1102 74-75 0.1622589623613564 1102 76-77 0.12166429251881539 1102 78-79 0.12997929605582925 1102 80-81 0.09652567492873487 1102 82-83 0.14980294624917434 1102 84-85 0.11812732881053023 1102 86-87 0.31295422878919865 1102 88-89 1.264131536074025 1102 90-91 0.15491423065488696 1102 92-93 0.10626171613843427 1102 94-95 0.1755994931927889 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.012182290448972566 1103 2 0.1644447555225632 1103 3 0.07894756210913911 1103 4 0.11360511987493283 1103 5 -0.015074648223063036 1103 6 0.03539803493227822 1103 7 0.0020815355317012063 1103 8 0.05091962931589222 1103 9 0.12466359920269099 1103 10-11 0.14761005658661475 1103 12-13 0.19879693933399523 1103 14-15 0.15395265946354897 1103 16-17 0.2712949923267942 1103 18-19 0.20381985235490419 1103 20-21 0.20004299172145323 1103 22-23 0.1934754680244879 1103 24-25 0.1846487131688832 1103 26-27 0.259870792195521 1103 28-29 0.22289369923292668 1103 30-31 0.07293258799874991 1103 32-33 0.2026224524951914 1103 34-35 0.2675858984310864 1103 36-37 0.17746613541488898 1103 38-39 0.1503517876119531 1103 40-41 0.15688546421649363 1103 42-43 0.21053485299849228 1103 44-45 0.08565022384905774 1103 46-47 0.0764449201172468 1103 48-49 0.06289444097220809 1103 50-51 0.006489903911223394 1103 52-53 -0.031339049512070005 1103 54-55 0.06471334534204232 1103 56-57 0.06551309640234138 1103 58-59 0.10284883386386667 1103 60-61 0.07899916862690759 1103 62-63 0.11125578635804345 1103 64-65 0.160529951912892 1103 66-67 0.17238052361084755 1103 68-69 0.11521756622261137 1103 70-71 0.27567845008094594 1103 72-73 0.3200756017496502 1103 74-75 0.24302781927240602 1103 76-77 0.27154424334857197 1103 78-79 0.06448224091388255 1103 80-81 0.11269214222980395 1103 82-83 0.06273440085101356 1103 84-85 0.21280681306527782 1103 86-87 0.281526870274309 1103 88-89 0.7962779993679412 1103 90-91 -0.47720829554201716 1103 92-93 -0.29101143853951594 1103 94-95 -0.0982604033751473 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.12640717109061583 1104 2 0.14776121585015645 1104 3 0.1945267040846943 1104 4 0.22644079321943877 1104 5 0.20282922132660275 1104 6 0.2705597368512116 1104 7 0.1740346212076247 1104 8 0.2533308586748788 1104 9 0.2865570893802385 1104 10-11 0.2961542940709663 1104 12-13 0.27407549129117115 1104 14-15 0.3496099498756635 1104 16-17 0.3072541779679696 1104 18-19 0.239098012748876 1104 20-21 0.23459349716429045 1104 22-23 0.1187851029423257 1104 24-25 0.17985248497511463 1104 26-27 0.2323769503689519 1104 28-29 0.28038780841716715 1104 30-31 0.2461782532783161 1104 32-33 0.246828464421867 1104 34-35 0.3580789964980866 1104 36-37 0.28221255508517373 1104 38-39 0.28794709693740117 1104 40-41 0.23513979125302598 1104 42-43 0.0693308466753706 1104 44-45 0.11023397216570885 1104 46-47 0.23199013924632794 1104 48-49 0.27733108151992525 1104 50-51 0.21989479647238852 1104 52-53 0.21489736498158152 1104 54-55 0.2930843729491457 1104 56-57 0.15281979628029774 1104 58-59 0.08564636768934264 1104 60-61 -0.0012146585644643437 1104 62-63 0.07324773573439103 1104 64-65 0.02211509659444033 1104 66-67 0.1931420045702481 1104 68-69 0.10934811802568589 1104 70-71 0.016160359124185675 1104 72-73 0.043692609992064035 1104 74-75 0.06459373104311084 1104 76-77 0.09003680501609779 1104 78-79 0.05876826014974057 1104 80-81 0.1619580559583298 1104 82-83 0.25781666175378604 1104 84-85 0.17673636873998788 1104 86-87 0.3121717432909925 1104 88-89 0.5054008535858046 1104 90-91 -0.5694335344850407 1104 92-93 -0.29101143853951594 1104 94-95 -0.0982604033751473 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.10941108798867916 1105 2 -0.27234818311023545 1105 3 -0.13283516556996489 1105 4 -0.032603156742524675 1105 5 -0.10889716800966909 1105 6 -0.11944426349259629 1105 7 -0.14220741726475694 1105 8 -0.33029259330051275 1105 9 -0.13394652016692277 1105 10-11 -0.1989495019982641 1105 12-13 -0.09279057671628266 1105 14-15 -0.2746453074034747 1105 16-17 -0.12185408675839682 1105 18-19 -0.06553871104987508 1105 20-21 0.008942477357770429 1105 22-23 -0.32595060928353803 1105 24-25 -0.1285300426798699 1105 26-27 -0.1017816881646425 1105 28-29 -0.18269827608969536 1105 30-31 -0.2741763073144412 1105 32-33 -0.2637592929195307 1105 34-35 -0.061702261810857806 1105 36-37 -0.20631979267483302 1105 38-39 -0.24045936048504757 1105 40-41 -0.19711362329746152 1105 42-43 -0.12970282529482802 1105 44-45 -0.28590258334548935 1105 46-47 -0.12646958379814777 1105 48-49 -0.11360000653342439 1105 50-51 -0.21884110291816228 1105 52-53 -0.24747369823429466 1105 54-55 -0.20092545359819525 1105 56-57 -0.15208672982274152 1105 58-59 -0.18899982680287053 1105 60-61 -0.27217660067515226 1105 62-63 -0.18291895891326249 1105 64-65 -0.17765819717458697 1105 66-67 -0.02357973376389566 1105 68-69 -0.1740353735675626 1105 70-71 -0.23155340021095583 1105 72-73 -0.14261426537795074 1105 74-75 -0.2437475280048531 1105 76-77 -0.21057788636701957 1105 78-79 -0.191418201658621 1105 80-81 -0.3210984195781279 1105 82-83 -0.2000536130409145 1105 84-85 -0.28272269721990284 1105 86-87 -0.2908223493740003 1105 88-89 0.9113405608538159 1105 90-91 -0.7313104990113093 1105 92-93 -0.29101143853951594 1105 94-95 -0.0982604033751473 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.18369571808726448 1106 2 -0.026806515475545467 1106 3 -0.05835615620723544 1106 4 -0.09436000454588367 1106 5 -0.13491378704954116 1106 6 -0.0765813124901058 1106 7 -0.13638997888917714 1106 8 -0.013793201219726825 1106 9 -0.03371341993382515 1106 10-11 -0.14044134349010307 1106 12-13 -0.09160223017909175 1106 14-15 0.016527473304186913 1106 16-17 -0.09733190351528975 1106 18-19 -0.03386592647011355 1106 20-21 -0.15355568339620618 1106 22-23 -0.15724237952879605 1106 24-25 -0.24321613178456403 1106 26-27 -0.38538800432909426 1106 28-29 -0.18066174672758706 1106 30-31 -0.07472420902513477 1106 32-33 -0.06877811131043643 1106 34-35 -0.18121220922778747 1106 36-37 -0.025567211922250976 1106 38-39 -0.04141305795036487 1106 40-41 0.08270849257117163 1106 42-43 -0.33900302122292914 1106 44-45 -0.06555684381370241 1106 46-47 -0.20282578573573318 1106 48-49 -0.13513254376363193 1106 50-51 -0.19437254821705352 1106 52-53 -0.20927391352287827 1106 54-55 -0.030883673672697398 1106 56-57 -0.27801196737588896 1106 58-59 -0.18244107547667454 1106 60-61 0.02171536007727326 1106 62-63 0.06180163057476307 1106 64-65 0.10874203050471465 1106 66-67 0.11664109192204819 1106 68-69 0.04924347936244544 1106 70-71 0.11785823466579615 1106 72-73 0.10642371214839841 1106 74-75 -0.05536478671513834 1106 76-77 0.07433313284632703 1106 78-79 0.08581948313720744 1106 80-81 0.08006843682128562 1106 82-83 0.1744242346927507 1106 84-85 0.11304761773645922 1106 86-87 0.20892654514233655 1106 88-89 0.6796451245072177 1106 90-91 -0.266158983859794 1106 92-93 -0.26503741256548974 1106 94-95 -0.0982604033751473 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.010243675852130707 1107 2 0.08141859274956431 1107 3 0.046405748554668946 1107 4 0.20044519025930896 1107 5 0.09235894022318547 1107 6 0.1342411983324041 1107 7 0.00863166613246591 1107 8 0.048111560685036636 1107 9 0.22342943720903463 1107 10-11 0.17860627555752018 1107 12-13 0.1876185490416873 1107 14-15 0.1624149191916331 1107 16-17 0.20028714410376125 1107 18-19 0.24470550210131137 1107 20-21 0.16917158933106435 1107 22-23 0.22695675467033993 1107 24-25 0.2102470716786371 1107 26-27 0.2529236839825941 1107 28-29 0.23275816669232796 1107 30-31 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-0.46728583158645876 2216 18-19 -0.3389130965116536 2216 20-21 -0.43744563786113844 2216 22-23 -0.40765478185777226 2216 24-25 -0.46482186539707016 2216 26-27 -0.43504392901320443 2216 28-29 -0.3911579960626099 2216 30-31 -0.4100727011943732 2216 32-33 -0.5856953917000993 2216 34-35 -0.5599471345805256 2216 36-37 -0.5448998522244537 2216 38-39 -0.6235443239035021 2216 40-41 -0.5799083344199474 2216 42-43 -0.44430446536316026 2216 44-45 -0.5266577872145319 2216 46-47 -0.5419844849846314 2216 48-49 -0.370374885037549 2216 50-51 -0.4236538456494259 2216 52-53 -0.4214956639633485 2216 54-55 -0.4389709077464019 2216 56-57 -0.5598033064389298 2216 58-59 -0.5199638308122871 2216 60-61 -0.5113600387816604 2216 62-63 -0.5495260120944749 2216 64-65 -0.4498586322673148 2216 66-67 -0.47473457487639337 2216 68-69 -0.4496581639022139 2216 70-71 -0.43224572000410433 2216 72-73 -0.3716760160076973 2216 74-75 -0.37178181575900027 2216 76-77 -0.3369000721298363 2216 78-79 -0.2694060692134883 2216 80-81 -0.43936547387530567 2216 82-83 -0.45460571694148655 2216 84-85 -0.4532107950270259 2216 86-87 -0.6376566161533397 2216 88-89 -1.2286951635399461 2216 90-91 -0.7313104990113093 2216 92-93 -0.29101143853951594 2216 94-95 -0.0982604033751473 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32221.0 3 4812.0 4 3274.0 5 2498.0 6 2442.0 7 2694.0 8 2991.0 9 3510.0 10 4030.0 11 4505.0 12 5269.0 13 5832.0 14 6551.0 15 6941.0 16 7971.0 17 9539.0 18 11755.0 19 14643.0 20 16693.0 21 22177.0 22 27202.0 23 34879.0 24 51922.0 25 84796.0 26 169950.0 27 461635.0 28 504823.0 29 17120.0 30 510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62902076898079 19.43742880871332 14.407639255901286 24.525911166404608 2 13.829252936461096 19.93451844401537 42.71900114037962 23.51722747914392 3 18.914740493700997 24.595861612402818 28.756841816287952 27.732556077608233 4 11.948544624126965 17.24455227439014 38.81483972409662 31.992063377386277 5 13.352481148290915 37.89546554082944 35.00253851948815 13.749514791391496 6 30.967938891204945 37.18097276430637 18.836910815166906 13.014177529321783 7 28.35781602366095 31.60489369314955 21.862544602264332 18.174745680925167 8 24.65662411328893 37.56201643267233 20.491404524072912 17.289954929965827 9 25.36461427863326 17.715707547014972 20.99554551810844 35.92413265624334 10-11 24.109689228296666 26.892033487922607 28.955649248675137 20.042628035105594 12-13 25.455395963306366 24.02819153039502 28.603898821687256 21.912513684611355 14-15 21.336180437701262 27.12589081431343 25.731017571732917 25.806911176252388 16-17 21.5938906700692 29.30083374995117 27.62719536810689 21.478080211872737 18-19 21.950376390557196 28.178162027016867 28.55161446944377 21.319847112982167 20-21 22.52853542706384 27.84787189447136 27.909192164530033 21.71440051393477 22-23 21.953613416689784 28.451378778901343 27.747258609059024 21.847749195349852 24-25 22.117635087005187 27.876324937548624 28.352137133703394 21.653902841742795 26-27 21.3630648936275 28.652494608337136 27.990460777909448 21.99397972012592 28-29 21.47891057358395 28.642924272659265 27.451526210002374 22.42663894375442 30-31 22.04013301076363 27.956847001513275 27.848849614459176 22.154170373263916 32-33 21.391677026095916 28.448311235223656 28.13606758801302 22.023944150667408 34-35 21.79472197365648 28.627485994788543 28.022141791235853 21.55565024031912 36-37 21.590037710480154 28.09470162501707 28.018052973245517 22.297207691257263 38-39 21.765478257729693 28.58323184642706 27.661019508464175 21.990270387379077 40-41 22.662578741255988 27.679795953872972 28.04885814920709 21.608767155663955 42-43 21.61789277074026 28.44237567990756 28.121895895771033 21.817835653581145 44-45 21.49415303732182 28.553937016145127 27.913567894620744 22.03834205191231 46-47 22.154859718287668 28.054766820839227 27.626978994672346 22.163394466200756 48-49 21.895942972090044 28.153295990504102 27.6728550194855 22.27790601792036 50-51 21.575003413901406 28.77501995819284 27.65329073834809 21.996685889557664 52-53 21.73360425686965 28.29482301887164 28.00178573187105 21.96978699238766 54-55 21.57038849054058 28.04230928438787 28.223051416967763 22.164250808103787 56-57 21.818032609302218 28.26938290489993 27.506737526958315 22.405846958839536 58-59 22.008521617531684 28.286583704540156 27.345463617354426 22.35943106057373 60-61 21.64641852434209 27.940401198803823 28.27558701011368 22.137593266740417 62-63 21.849840958255236 27.87369886126767 28.255924263960058 22.020535916517034 64-65 21.87786304145233 28.14221004888774 27.705033569494418 22.274893340165516 66-67 21.814717187997516 28.60726044439776 27.366242445927448 22.211779921677273 68-69 22.265636540419738 27.767944542302647 28.164910361815647 21.801508555461965 70-71 22.37682881593503 27.764027350584463 27.795638743816408 22.063505089664094 72-73 22.022144388239116 28.212922264859486 27.633018970118535 22.13191437678286 74-75 21.6729747207332 28.5104238815377 28.022663038304607 21.793938359424494 76-77 21.974152844204742 27.772890358032676 28.199299494152058 22.053657303610528 78-79 21.88543048096745 27.832035609711266 27.953951601255266 22.328582308066018 80-81 22.039345187879345 28.18666150204998 27.775713390034696 21.998279920035976 82-83 21.813791464402623 28.22005233807825 28.255373319134915 21.710782878384215 84-85 21.81666824231347 28.05314394058761 28.224232922614732 21.905954894484186 86-87 21.445228408366493 28.68174354685062 27.55268449832619 22.3203435464567 88-89 31.10238333373991 17.752543117117554 33.77076574049228 17.374307808650258 90-91 30.768675616617774 13.480524166872707 15.791785557567698 39.95901465894182 92-93 23.096652297613765 16.433252328073507 34.22119071110258 26.248904663210148 94-95 15.807933109635137 6.572999992855628 26.458523353758295 51.16054354375093 96-97 26.772865022490606 7.227100460167307 13.940020365231831 52.060014152110256 98-99 57.60300624395727 14.23250763229712 18.505165195772587 9.659320927973024 100-101 30.931506457472217 7.149361267698201 11.679160715903231 50.23997155892634 102-103 23.79497681108124 2.114672082564349 35.77371772723315 38.31663337912127 104-105 43.438104036849936 5.333411960502952 36.855044260544226 14.373439742102883 106-107 5.611580710292172 1.5817144414598896 5.995309188324613 86.81139565992333 108-109 17.685925534446532 9.676453123275722 19.869729948449002 52.76789139382875 110-111 40.03925306170114 2.5595751585271547 23.17362133980268 34.22755043996902 112-113 28.550774408461827 2.9840997286994746 36.74988838902435 31.71523747381435 114-115 29.261072214642112 4.132099712177196 21.62380862273302 44.98301945044767 116-117 20.011407554554665 4.202602102530314 18.850295514667557 56.93569482824746 118-119 20.598986190158037 4.358654312449288 14.024134639207459 61.01822485818522 120-121 26.07064607851545 4.364472827978632 27.906832057923932 41.65804903558199 122-123 34.76286385914406 1.5932221015097572 32.31364747156036 31.330266567785824 124-125 38.47375532880461 1.798745110531765 18.293374017745897 41.434125542917734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 8.0 2 26.0 3 50.5 4 69.5 5 72.0 6 78.0 7 80.5 8 77.0 9 83.5 10 87.5 11 86.5 12 88.0 13 88.5 14 111.5 15 167.5 16 219.0 17 288.5 18 408.0 19 591.5 20 895.5 21 1364.0 22 2067.0 23 3083.5 24 4555.5 25 6429.5 26 8662.0 27 11438.0 28 14775.0 29 18540.0 30 22923.0 31 28075.0 32 33682.0 33 39697.5 34 45871.0 35 51091.0 36 54897.0 37 57183.0 38 58562.0 39 59642.0 40 60421.5 41 61340.0 42 62151.0 43 61878.5 44 61473.0 45 61079.0 46 59887.0 47 58382.0 48 56670.5 49 54745.0 50 52226.5 51 49141.0 52 45627.5 53 41455.0 54 36708.0 55 32272.0 56 28103.0 57 23886.0 58 20020.0 59 16507.0 60 13117.5 61 10233.0 62 7944.5 63 6090.0 64 4549.5 65 3255.5 66 2317.5 67 1623.0 68 1175.0 69 848.0 70 566.0 71 357.5 72 249.5 73 185.0 74 140.0 75 104.0 76 69.0 77 46.0 78 29.5 79 22.5 80 21.5 81 23.0 82 17.0 83 11.5 84 11.5 85 10.0 86 5.5 87 4.5 88 6.5 89 7.0 90 9.0 91 9.0 92 6.0 93 3.5 94 2.5 95 2.5 96 4.0 97 8.0 98 8.0 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.252152561901542E-4 3 0.0369620236543821 4 0.04090113807580826 5 0.04227982812330741 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.908671632139235E-4 12-13 0.0021336869782725013 14-15 0.0 16-17 4.267373956545003E-4 18-19 0.006532364748865042 20-21 0.00226499079232004 22-23 4.923893026782695E-4 24-25 0.0 26-27 0.0 28-29 0.001083256465892193 30-31 0.0 32-33 0.0010176045588684238 34-35 1.3130381404753854E-4 36-37 6.565190702376927E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.565190702376927E-5 46-47 0.0 48-49 6.565190702376927E-5 50-51 6.565190702376927E-5 52-53 0.0 54-55 0.0013458640939872702 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.847786053565391E-5 66-67 0.0 68-69 3.9391144214261564E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.2825953511884633E-4 80-81 0.0 82-83 7.22170977261462E-4 84-85 6.565190702376927E-5 86-87 9.191266983327699E-4 88-89 19.261941261238785 90-91 26.315418022104996 92-93 40.51188791906433 94-95 44.86408413948404 96-97 48.646060721448805 98-99 93.10776432278416 100-101 51.8016852844533 102-103 53.2572865410308 104-105 74.9506790048484 106-107 81.6934252897711 108-109 12.54647334368445 110-111 39.00284601016948 112-113 52.20705298437156 114-115 86.8159153353007 116-117 66.62027265236988 118-119 72.04413777709208 120-121 80.47804436099358 122-123 76.00928317965317 124-125 50.126314269113735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1523185.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.97201947414332 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9128330670423 62.072017217657915 2 10.791349350917566 15.965158086767161 3 2.764026775214697 6.133819273297054 4 1.0112012389088088 2.9920239094736107 5 0.47893461829943973 1.771388045584378 6 0.2689021924636525 1.1934742930536681 7 0.17330897891869518 0.8974010614532331 8 0.11632682867249614 0.6883944348741775 9 0.08478094801149927 0.5644276143608661 >10 0.36916388737198186 4.692598271748139 >50 0.0174646582162539 0.8845555521895886 >100 0.01055455715221541 1.517983279784756 >500 9.755297627508855E-4 0.47102044494919193 >1k 1.7736904777288827E-4 0.15573851480632056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.565190702376927E-5 0.0 5 0.0 0.0 0.0 6.565190702376927E-5 0.0 6 0.0 0.0 0.0 6.565190702376927E-5 0.0 7 0.0 0.0 0.0 6.565190702376927E-5 0.0 8 0.0 0.0 0.0 6.565190702376927E-5 0.0 9 0.0 0.0 0.0 6.565190702376927E-5 0.0 10-11 0.0 0.0 0.0 1.3130381404753854E-4 0.0 12-13 0.0 0.0 0.0 1.3130381404753854E-4 1.3130381404753854E-4 14-15 0.0 0.0 0.0 1.9695572107130782E-4 1.3130381404753854E-4 16-17 0.0 0.0 0.0 1.9695572107130782E-4 1.3130381404753854E-4 18-19 0.0 0.0 0.0 1.9695572107130782E-4 1.3130381404753854E-4 20-21 0.0 0.0 0.0 1.9695572107130782E-4 1.9695572107130782E-4 22-23 0.0 0.0 0.0 1.9695572107130782E-4 1.9695572107130782E-4 24-25 0.0 0.0 0.0 2.2978167458319245E-4 1.9695572107130782E-4 26-27 0.0 0.0 0.0 4.2673739565450027E-4 1.9695572107130782E-4 28-29 0.0 0.0 0.0 9.191266983327698E-4 1.9695572107130782E-4 30-31 0.0 0.0 0.0 0.0021336869782725013 1.9695572107130782E-4 32-33 0.0 0.0 0.0 0.006039975446186773 1.9695572107130782E-4 34-35 0.0 0.0 0.0 0.01083256465892193 2.626076280950771E-4 36-37 0.0 0.0 0.0 0.020516220944927898 2.626076280950771E-4 38-39 0.0 0.0 0.0 0.038800277051047646 2.626076280950771E-4 40-41 0.0 0.0 0.0 0.05856150106520219 2.626076280950771E-4 42-43 6.565190702376927E-5 0.0 0.0 0.0788479403355469 2.626076280950771E-4 44-45 6.565190702376927E-5 0.0 0.0 0.10205588946844933 2.626076280950771E-4 46-47 6.565190702376927E-5 0.0 0.0 0.12939990874384924 2.626076280950771E-4 48-49 6.565190702376927E-5 0.0 0.0 0.1623571660697814 2.626076280950771E-4 50-51 6.565190702376927E-5 1.3130381404753854E-4 0.0 0.2002383164224963 2.626076280950771E-4 52-53 6.565190702376927E-5 1.3130381404753854E-4 0.0 0.23618273551800995 2.626076280950771E-4 54-55 6.565190702376927E-5 1.3130381404753854E-4 0.0 0.27429366754530804 2.626076280950771E-4 56-57 6.565190702376927E-5 1.3130381404753854E-4 0.0 0.3200202207873633 2.626076280950771E-4 58-59 6.565190702376927E-5 1.641297675594232E-4 0.0 0.3704408853816181 2.626076280950771E-4 60-61 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.4273282628177142 2.626076280950771E-4 62-63 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.48917235923410485 2.626076280950771E-4 64-65 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.5502614587197221 2.626076280950771E-4 66-67 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.6215922557010475 2.626076280950771E-4 68-69 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.7070053867389713 2.626076280950771E-4 70-71 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.8019052183418298 2.626076280950771E-4 72-73 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.8939491919891542 2.626076280950771E-4 74-75 6.565190702376927E-5 1.9695572107130782E-4 0.0 0.9954798662014135 2.626076280950771E-4 76-77 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.103477253255514 2.626076280950771E-4 78-79 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1311495320660327 2.626076280950771E-4 80-81 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 82-83 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 84-85 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 86-87 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 88-89 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 90-91 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 92-93 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 94-95 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 96-97 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 98-99 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 100-101 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 102-103 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 104-105 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 106-107 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 108-109 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 110-111 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 112-113 6.565190702376927E-5 1.9695572107130782E-4 0.0 1.1317075732757347 2.626076280950771E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 450 0.0 1984.1233 108-109 CCGCGCC 440 0.0 1972.8497 108-109 CGTCGCT 35 3.931491E-6 1766.5011 110-111 CGTCGCG 25 0.0053419857 1236.5507 110-111 CCGCGCG 165 0.0 1202.4989 108-109 CCGCTCG 145 0.0 1197.3158 108-109 CGCGTCT 130 0.0 1188.9911 110-111 CCGCGCT 75 5.630318E-8 992.0616 108-109 CCGCGAC 25 0.008302244 992.0615 108-109 CATCGCG 80 7.287417E-8 930.05774 108-109 CCGCGCA 55 2.956118E-5 901.87415 108-109 CCGTTCG 55 2.956118E-5 901.87415 108-109 CGTCGTC 105 8.933057E-8 883.25055 110-111 CGGGTCG 40 0.006864142 772.8442 110-111 CCGTGCG 105 2.1608685E-7 708.6154 108-109 CGTCTCG 140 2.8207432E-7 662.43787 110-111 CGTCGCC 150 7.3305273E-10 661.3744 108-109 CGTGTCG 95 7.850483E-5 650.81616 110-111 CCGTTGC 115 3.108289E-7 646.9967 108-109 CGCCGCC 305 0.0 608.13965 110-111 >>END_MODULE