FastQCFastQC Report
Fri 27 May 2016
SRR939943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523185
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24240.15914022262561672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.11528474873373884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39750.047.9437181
ATCAACG46950.040.2068183
GGTATCA24450.039.7033921
CAACGCA48300.038.9597365
TCAACGC48450.038.8391154
AACGCAG50200.037.3334246
TATCAAC50750.037.31362
CGCAGAG58550.031.7043368
ACGCAGA59150.031.5839087
GTACATG47550.026.8029211
TACATGG45400.026.4954662
GCAGAGT70050.025.9049439
ACATGGG43850.025.5307943
GTCCTAC32000.025.1248021
AGAGTAC58750.024.76064710-11
TAGGACC14350.024.483654
GGACCGA2951.8189894E-1024.201946
GTAGGAC34750.023.991053
GAGTACT37650.023.62474612-13
AGTACTT43200.022.79319612-13
Produced by FastQC (version 0.11.5)