FastQCFastQC Report
Fri 27 May 2016
SRR939942_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939942_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009865
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20880.2067603095463255No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18000.17824164616062543No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12210.12090724997895759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAC153.6524762E-73896.9998110-111
CCGCGCT256.6211214E-103009.1619108-109
CCGCTCC2050.02568.7966108-109
CCGCGCC1800.01950.3827108-109
CGCGCGA150.00214967021948.4999110-111
CCGCGCG750.01671.7566108-109
CGTCGTC354.6376463E-61670.1428110-111
CGCCGCT354.6376463E-61670.1428110-111
CTCCGCC1700.01547.3383110-111
CCGCTCG501.0586518E-81504.5809108-109
CGTCGCG200.00382120351461.3749110-111
CGCGTCG200.00382120351461.3749110-111
CATCGCG200.0051931541253.8174108-109
CGTCGAT200.0051931541253.8174108-109
CGCGACG250.00596995331169.1001110-111
TTGCGCT250.00596995331169.1001110-111
CAGCGCG451.562242E-51114.5044108-109
CCGTTCG451.562242E-51114.5044108-109
CCCGTAC250.008113231003.05396108-109
CGTACGT300.00859576974.24994110-111
Produced by FastQC (version 0.11.5)