##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939942_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1009865 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47308600654543 33.0 31.0 34.0 30.0 34.0 2 31.642658177083074 34.0 31.0 34.0 30.0 34.0 3 31.849891817223096 34.0 31.0 34.0 30.0 34.0 4 35.103451451431624 37.0 35.0 37.0 33.0 37.0 5 35.199116713620135 37.0 35.0 37.0 33.0 37.0 6 35.25858307793616 37.0 37.0 37.0 33.0 37.0 7 35.19636684111243 37.0 36.0 37.0 33.0 37.0 8 35.25521134012962 37.0 36.0 37.0 33.0 37.0 9 36.93113336931174 39.0 38.0 39.0 34.0 39.0 10-11 36.89937862981686 39.0 38.0 39.0 34.0 39.0 12-13 36.8590182846222 39.0 38.0 39.0 34.0 39.0 14-15 38.346184390982955 41.0 39.0 41.0 34.0 41.0 16-17 38.33766394518079 41.0 39.0 41.0 34.0 41.0 18-19 38.32212622479243 41.0 39.0 41.0 34.0 41.0 20-21 38.335559208409045 41.0 39.0 41.0 34.0 41.0 22-23 38.30710788075634 41.0 39.0 41.0 34.0 41.0 24-25 38.30450159179692 41.0 39.0 41.0 34.0 41.0 26-27 38.24454308249122 41.0 39.0 41.0 34.0 41.0 28-29 38.18450238398202 41.0 39.0 41.0 33.5 41.0 30-31 38.09428735524055 40.0 39.0 41.0 33.0 41.0 32-33 37.99389175780921 40.0 38.0 41.0 33.0 41.0 34-35 37.95554504810049 40.0 38.0 41.0 33.0 41.0 36-37 38.135024978586245 41.0 39.0 41.0 33.0 41.0 38-39 38.08577532640501 41.0 39.0 41.0 33.0 41.0 40-41 37.99537710486055 41.0 39.0 41.0 33.0 41.0 42-43 37.9564634876939 41.0 39.0 41.0 33.0 41.0 44-45 37.88295415723884 41.0 38.0 41.0 33.0 41.0 46-47 37.77432082506077 40.0 38.0 41.0 33.0 41.0 48-49 37.68494501740332 40.0 38.0 41.0 32.5 41.0 50-51 37.576757289340655 40.0 38.0 41.0 32.0 41.0 52-53 37.482849687829564 40.0 38.0 41.0 32.0 41.0 54-55 37.36293266921816 40.0 38.0 41.0 31.5 41.0 56-57 37.18323439271586 40.0 38.0 41.0 31.0 41.0 58-59 37.032401855693585 40.0 37.0 41.0 31.0 41.0 60-61 36.84204621409792 40.0 37.0 41.0 31.0 41.0 62-63 36.605806716739366 40.0 36.5 41.0 30.0 41.0 64-65 36.34694191797914 39.5 36.0 41.0 30.0 41.0 66-67 36.06292870829269 39.0 35.5 41.0 29.5 41.0 68-69 35.66255836176123 39.0 35.0 41.0 29.0 41.0 70-71 35.25185693137202 38.5 35.0 40.0 28.5 41.0 72-73 34.8621073113733 37.0 35.0 40.0 28.0 41.0 74-75 34.44209325008788 37.0 34.5 39.0 28.0 41.0 76-77 33.95499101365034 36.5 34.0 39.0 26.5 40.5 78-79 33.46883741886292 36.0 34.0 38.5 26.0 40.0 80-81 32.99233263852099 35.5 34.0 37.0 26.0 39.0 82-83 32.741878864996806 35.0 34.0 37.0 26.0 39.0 84-85 32.26288167230273 35.0 34.0 37.0 24.5 39.0 86-87 31.556460021884114 35.0 34.0 36.0 4.5 37.0 88-89 16.03048625311304 18.0 16.5 18.5 2.0 19.5 90-91 2.761604273838582 2.0 2.0 2.0 2.0 2.0 92-93 2.2963648606496907 2.0 2.0 2.0 2.0 2.0 94-95 2.100895664271957 2.0 2.0 2.0 2.0 2.0 96-97 2.0000292118253427 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34280837098560113 1101 2 0.3575190590967168 1101 3 0.3804049409848105 1101 4 0.30560901385715056 1101 5 0.40072522336174643 1101 6 0.4614906699614991 1101 7 0.3673615298414106 1101 8 0.4492625601283535 1101 9 0.4346107435433666 1101 10-11 0.41963306274705303 1101 12-13 0.4696467929529362 1101 14-15 0.513772117796627 1101 16-17 0.5639928172100213 1101 18-19 0.5974924092293179 1101 20-21 0.5591046315786627 1101 22-23 0.6119463829191503 1101 24-25 0.5574602929683721 1101 26-27 0.5775798026835943 1101 28-29 0.5920264561163009 1101 30-31 0.6085620055373298 1101 32-33 0.6336282114159104 1101 34-35 0.5687727451261466 1101 36-37 0.6175767015811076 1101 38-39 0.5792992526284308 1101 40-41 0.5259973815673931 1101 42-43 0.5302067652119362 1101 44-45 0.57817046773161 1101 46-47 0.6532151642554211 1101 48-49 0.5318388171826882 1101 50-51 0.5086937462078254 1101 52-53 0.48175752760349155 1101 54-55 0.4248033505080713 1101 56-57 0.45864894960620717 1101 58-59 0.3622480476357808 1101 60-61 0.41025921707681334 1101 62-63 0.3951643415664847 1101 64-65 0.35081900843825053 1101 66-67 0.40177462212536597 1101 68-69 0.35268381492686984 1101 70-71 0.3053511632063959 1101 72-73 0.3546785143781861 1101 74-75 0.41101732202862706 1101 76-77 0.3776673260579244 1101 78-79 0.4410345603898236 1101 80-81 0.5472456629542108 1101 82-83 0.4058828168673756 1101 84-85 0.4533408627987825 1101 86-87 0.5179490302777054 1101 88-89 0.40571179808075186 1101 90-91 -0.11869886328390766 1101 92-93 -0.17503377810060927 1101 94-95 -0.016198096887084734 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.07877529647891635 1102 2 0.009284491383699134 1102 3 0.163541098806526 1102 4 0.2045730801447121 1102 5 0.28687828085264044 1102 6 0.1973513944290488 1102 7 0.3102889382538905 1102 8 0.26339287891237007 1102 9 0.3875287378415351 1102 10-11 0.20752820780825942 1102 12-13 0.17160765386797294 1102 14-15 0.2023364463072923 1102 16-17 0.146066647496788 1102 18-19 0.1312012272434373 1102 20-21 0.13887543410332626 1102 22-23 0.1893422178405686 1102 24-25 0.19273196829958295 1102 26-27 0.2835278459199131 1102 28-29 0.15169126409886502 1102 30-31 0.13367663286037157 1102 32-33 0.15595910038272365 1102 34-35 0.198410720977229 1102 36-37 0.23604494514368213 1102 38-39 0.2518653038839034 1102 40-41 0.2896068749584515 1102 42-43 0.2733510412779836 1102 44-45 0.2904411178009738 1102 46-47 0.25094567665765766 1102 48-49 0.2211439831302755 1102 50-51 0.2576203656641667 1102 52-53 0.18126290546392454 1102 54-55 0.23514610811940173 1102 56-57 0.15923866573445622 1102 58-59 0.21341833257472587 1102 60-61 0.26764014949338133 1102 62-63 0.23053835251277377 1102 64-65 0.2101413637200693 1102 66-67 0.17106212231593076 1102 68-69 0.15620086323995253 1102 70-71 0.19702119659410045 1102 72-73 0.22695004106548566 1102 74-75 0.25876689134243236 1102 76-77 0.1983698972164305 1102 78-79 0.1984686615896436 1102 80-81 0.16106497294155986 1102 82-83 0.17210120145109897 1102 84-85 0.19043885724940424 1102 86-87 0.2532532929276847 1102 88-89 0.8098107491883404 1102 90-91 0.011460319098358873 1102 92-93 -0.006483970956554064 1102 94-95 0.11153418780792856 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.16459917358976384 1103 2 0.0950258014294505 1103 3 -0.019069726836601575 1103 4 0.10140369748031475 1103 5 0.032374018098551005 1103 6 -0.0063764168189663906 1103 7 0.18079121205912685 1103 8 0.08721448634634044 1103 9 0.12207608225536859 1103 10-11 0.10678193915154566 1103 12-13 0.17692153256594878 1103 14-15 0.036211740681807214 1103 16-17 0.1829410987698381 1103 18-19 0.1874555448128774 1103 20-21 0.17610285957967164 1103 22-23 0.1714052110067641 1103 24-25 0.22245657044100398 1103 26-27 0.24825198476835908 1103 28-29 0.22893688185187955 1103 30-31 0.3363989607378599 1103 32-33 0.2807933928701303 1103 34-35 0.24618654772584136 1103 36-37 0.15529435547722414 1103 38-39 0.1918743679128312 1103 40-41 0.22032897898745318 1103 42-43 0.2170790635307256 1103 44-45 0.2397834711274669 1103 46-47 0.2126203385684633 1103 48-49 0.09667275637634987 1103 50-51 0.12462317202697193 1103 52-53 0.1360361074972758 1103 54-55 0.09849486314566747 1103 56-57 0.02476535645565292 1103 58-59 -0.004596103817902986 1103 60-61 0.140047887678719 1103 62-63 0.1630652349730468 1103 64-65 0.0739463017810067 1103 66-67 -0.0012525903093916213 1103 68-69 -0.041418590661479016 1103 70-71 0.003054876617369473 1103 72-73 0.09335400685336737 1103 74-75 0.06831357063268229 1103 76-77 0.1213706045409495 1103 78-79 0.12474223563349796 1103 80-81 0.14423459413303874 1103 82-83 0.3024321669444845 1103 84-85 0.38385518883140435 1103 86-87 0.2744211948893849 1103 88-89 0.6311046576654995 1103 90-91 -0.5320591754444166 1103 92-93 -0.31618546349386767 1103 94-95 -0.11065034407809593 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.18931339232343092 1104 2 -0.0011571314517304643 1104 3 0.14928453190803737 1104 4 0.057224988487107 1104 5 0.18736678425668174 1104 6 0.12494166723786293 1104 7 0.13639427798455728 1104 8 0.27919640379608524 1104 9 0.2374637353724509 1104 10-11 0.2587063145735584 1104 12-13 0.3372409538220751 1104 14-15 0.3375303588879035 1104 16-17 0.3972835541854991 1104 18-19 0.26170777009238577 1104 20-21 0.13589306218659658 1104 22-23 0.14137438100729582 1104 24-25 0.1295636774110136 1104 26-27 0.20807734078796614 1104 28-29 0.19392271320041488 1104 30-31 0.18253338647490125 1104 32-33 0.21219264935262316 1104 34-35 0.28809649198713316 1104 36-37 0.23455799648509412 1104 38-39 0.1972284152804491 1104 40-41 0.23758336962586668 1104 42-43 0.21108449359164183 1104 44-45 0.13325561935251073 1104 46-47 0.2669777866863612 1104 48-49 0.3410426177577648 1104 50-51 0.2229248969638391 1104 52-53 0.24861833566666292 1104 54-55 0.18325544786105752 1104 56-57 0.29688860888592217 1104 58-59 0.26573386802741084 1104 60-61 0.19742236602718322 1104 62-63 0.23694629499910036 1104 64-65 0.29244690372613036 1104 66-67 0.14648761592700055 1104 68-69 0.10792885699531496 1104 70-71 0.20490326131805148 1104 72-73 0.3023558827672943 1104 74-75 0.31200615814756105 1104 76-77 0.22150647019830672 1104 78-79 0.2393478394527122 1104 80-81 0.3091075236769285 1104 82-83 0.3418265877783142 1104 84-85 0.23843876013508236 1104 86-87 0.2719097803023658 1104 88-89 0.5103314560707233 1104 90-91 -0.6201476926506428 1104 92-93 -0.31618546349386767 1104 94-95 -0.11065034407809593 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.2858534809889761 1105 2 -0.20819471021978941 1105 3 -0.15367946423252832 1105 4 -0.05998125032334656 1105 5 -0.16666226647166837 1105 6 -0.13396788050992825 1105 7 -0.10800710286636672 1105 8 -0.09937135257460739 1105 9 -0.0278708059942403 1105 10-11 -0.05541645226936964 1105 12-13 0.04693822741990772 1105 14-15 -0.17481796564464958 1105 16-17 -0.08071999569475707 1105 18-19 -0.06704241319439319 1105 20-21 -0.02854165423029542 1105 22-23 -0.12674611941539382 1105 24-25 -0.0017280373024348705 1105 26-27 -0.04899413472038816 1105 28-29 -0.10656170697072298 1105 30-31 -0.0641955102020475 1105 32-33 -0.06866039900069154 1105 34-35 0.029494911285738112 1105 36-37 -0.044416475513209264 1105 38-39 -0.04016937405469889 1105 40-41 -0.03463121439007466 1105 42-43 -0.031886571535359565 1105 44-45 -0.07639639825026023 1105 46-47 0.07355999963210991 1105 48-49 -0.0024046762052662984 1105 50-51 0.008203302794257183 1105 52-53 -0.10607326282938345 1105 54-55 -0.16109108272384987 1105 56-57 -0.030298658611407348 1105 58-59 -0.1593672338655594 1105 60-61 -0.1576629596259025 1105 62-63 -0.13873367632670153 1105 64-65 -0.1431139900254692 1105 66-67 -0.051372499602500454 1105 68-69 -0.005598748256545605 1105 70-71 -0.08585700319419232 1105 72-73 -0.01810272338425989 1105 74-75 -0.057754329024177764 1105 76-77 -0.02694849013028744 1105 78-79 -0.08252931586405765 1105 80-81 -0.2864629552944251 1105 82-83 -0.16038258362061697 1105 84-85 -0.20317648063586802 1105 86-87 -0.0045172317339208234 1105 88-89 0.7447955255260048 1105 90-91 -0.761606166654694 1105 92-93 -0.31618546349386767 1105 94-95 -0.11065034407809593 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.12491272837983658 1106 2 -0.086029685445979 1106 3 0.01773882097210233 1106 4 -0.03534977286654595 1106 5 0.012932874301036179 1106 6 0.022633269079470608 1106 7 -0.20960263846890825 1106 8 -0.014804175978142098 1106 9 -0.015549559036990956 1106 10-11 -0.1118242409931014 1106 12-13 -0.07515320741642739 1106 14-15 -0.07962629690688772 1106 16-17 -0.17980399926938162 1106 18-19 -0.07269011040220619 1106 20-21 -0.17272351038884182 1106 22-23 -0.13463610642478585 1106 24-25 -0.18027969920117926 1106 26-27 -0.38190685969848914 1106 28-29 -0.21417663506409923 1106 30-31 -0.10796213403671828 1106 32-33 -0.01608479525474138 1106 34-35 -0.21177454598186074 1106 36-37 -0.03821090488714418 1106 38-39 -0.04151404288235483 1106 40-41 -0.005095870949801906 1106 42-43 -0.407468681160573 1106 44-45 -0.0778514519841309 1106 46-47 -0.2593908422896476 1106 48-49 -0.17837848700068548 1106 50-51 -0.12436963056234163 1106 52-53 -0.1020178844184727 1106 54-55 0.015088515501531674 1106 56-57 -0.2939031025623109 1106 58-59 -0.10122170910320705 1106 60-61 -0.041803755243293494 1106 62-63 -0.04648450344234334 1106 64-65 -0.03973731354093246 1106 66-67 0.029232718144399428 1106 68-69 -0.031652009744952636 1106 70-71 0.06629393814009177 1106 72-73 -0.02159185586906176 1106 74-75 -0.14596442785502006 1106 76-77 -0.05034171774088492 1106 78-79 0.05659554554890178 1106 80-81 0.10098211664745804 1106 82-83 0.06095016980637524 1106 84-85 -0.14382156914649613 1106 86-87 -0.020850011600415996 1106 88-89 0.4127171870545965 1106 90-91 -0.37107362227599605 1106 92-93 -0.27772392503232934 1106 94-95 -0.11065034407809593 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.15100869249212678 1107 2 -0.06573503245283518 1107 3 -0.00772504926050388 1107 4 -0.010679508127019233 1107 5 0.05081960899706672 1107 6 -0.10392038173834095 1107 7 -0.05044393639653322 1107 8 -0.016092040832667465 1107 9 0.10071681673965571 1107 10-11 0.06080123175604513 1107 12-13 0.058887056329503196 1107 14-15 0.1394973968890767 1107 16-17 0.10433319258485341 1107 18-19 0.05868817135944937 1107 20-21 -0.05471834793648611 1107 22-23 0.03341482469812007 1107 24-25 -0.039168017302955604 1107 26-27 -0.02534841004085564 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-0.761606166654694 2205 92-93 -0.31618546349386767 2205 94-95 -0.11065034407809593 2205 96-97 0.0 2205 98-99 0.0 2205 100-101 0.0 2205 102-103 0.0 2205 104-105 0.0 2205 106-107 0.0 2205 108-109 0.0 2205 110-111 0.0 2205 112-113 0.0 2205 114-115 0.0 2205 116-117 0.0 2205 118-119 0.0 2205 120-121 0.0 2205 122-123 0.0 2205 124-125 0.0 2206 1 0.10049640202182175 2206 2 0.20143674236771147 2206 3 0.1392213651966756 2206 4 0.1516376719394401 2206 5 0.14343229456293471 2206 6 0.22026429143097914 2206 7 0.10961705562841928 2206 8 0.1483508146795529 2206 9 0.1858562563108137 2206 10-11 0.08836264913145442 2206 12-13 0.05437058826739616 2206 14-15 0.16197872992664486 2206 16-17 0.1746277935415108 2206 18-19 0.04396781505575831 2206 20-21 0.15208747473436546 2206 22-23 0.2535688984786546 2206 24-25 0.17354791535599645 2206 26-27 0.22280621508001985 2206 28-29 0.23229127809452166 2206 30-31 0.300481741458519 2206 32-33 0.2612462340985431 2206 34-35 0.2069905612267462 2206 36-37 0.08176867987682357 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-0.03602047254238272 2216 8 -0.040998049746967524 2216 9 -0.20392697524973613 2216 10-11 -0.1158044732441681 2216 12-13 -0.2257365385947807 2216 14-15 -0.1782334800927856 2216 16-17 -0.36077340729480767 2216 18-19 -0.3317513734975819 2216 20-21 -0.2893593805545578 2216 22-23 -0.3643981819052087 2216 24-25 -0.3603207063486309 2216 26-27 -0.3747310007072855 2216 28-29 -0.27920995092257783 2216 30-31 -0.3135140849127751 2216 32-33 -0.43045289102011 2216 34-35 -0.3276642083095922 2216 36-37 -0.2930068917817792 2216 38-39 -0.3619279306153942 2216 40-41 -0.5296123933117514 2216 42-43 -0.5037831706399984 2216 44-45 -0.4802051901543223 2216 46-47 -0.5055939785175383 2216 48-49 -0.5290897059387305 2216 50-51 -0.6055955193705103 2216 52-53 -0.7253698210325297 2216 54-55 -0.6568523458131281 2216 56-57 -0.6831032868711446 2216 58-59 -0.5898526675012832 2216 60-61 -0.4563916580956118 2216 62-63 -0.606853677477055 2216 64-65 -0.6031160031458995 2216 66-67 -0.5883068686809736 2216 68-69 -0.5980245424006085 2216 70-71 -0.46921744441905844 2216 72-73 -0.42979144383852486 2216 74-75 -0.561651953861606 2216 76-77 -0.416042330301309 2216 78-79 -0.4728593877292653 2216 80-81 -0.527492187081279 2216 82-83 -0.48517679797640767 2216 84-85 -0.6086908329063796 2216 86-87 -0.7292026417234716 2216 88-89 -1.1286263318492065 2216 90-91 -0.761606166654694 2216 92-93 -0.31618546349386767 2216 94-95 -0.11065034407809593 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15804.0 3 2349.0 4 1551.0 5 1351.0 6 1305.0 7 1325.0 8 1518.0 9 1879.0 10 2182.0 11 2477.0 12 2823.0 13 3252.0 14 3857.0 15 4248.0 16 4849.0 17 5993.0 18 7472.0 19 9193.0 20 10217.0 21 13526.0 22 16377.0 23 21053.0 24 31006.0 25 50952.0 26 93321.0 27 268614.0 28 415993.0 29 14822.0 30 556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.46039322087606 19.997425398444346 14.38925004827378 24.152931332405817 2 12.487102147034532 20.03242039969976 46.17560092607079 21.304876527194914 3 19.61606549354446 24.876689421790037 28.9662714982421 26.540973586423405 4 11.201915646560435 17.48671451159731 40.351426431484136 30.959943410358125 5 12.219998612996227 38.62637090462368 36.07408581590497 13.07954466647513 6 29.50919182266937 38.11588677694544 19.938407608937826 12.436513791447371 7 27.86530872938462 31.33290093230283 22.22138602684517 18.580404311467376 8 22.817406286978954 40.83020997856149 20.949532858352356 15.402850876107202 9 25.271397662063745 17.825253870566858 21.705376461210165 35.19797200615924 10-11 23.694377451778898 27.105218727270834 30.42535377656264 18.775050044387626 12-13 25.786755181988507 24.296525007822993 29.765042917519935 20.151676892668572 14-15 20.034212493749166 29.585786218950055 26.157159620345293 24.222841666955482 16-17 19.691541401778466 30.447289723327987 30.27959321094013 19.58157566395342 18-19 20.737218575444235 28.859308642195376 30.563762662836186 19.839710119524202 20-21 21.503526799816605 29.020269567723172 28.82711996664815 20.649083665812075 22-23 20.554326831908128 30.70523456078617 28.87779489243758 19.862643714868117 24-25 21.137577795051815 29.06195382551133 30.268006119629852 19.532462259807005 26-27 19.375560099617275 30.49184792026657 30.12224406232516 20.01034791779099 28-29 19.579443532595107 30.22500810761968 28.25100695398585 21.944541405799363 30-31 20.72316596772836 29.100622360414512 29.396800562451418 20.779411109405714 32-33 19.791275725380725 30.099747586025238 30.167381460000453 19.94159522859358 34-35 19.853623781827938 30.630525808686027 30.054358831663887 19.46149157782215 36-37 19.315411412648462 29.938248988475653 29.77436521029606 20.971974388579824 38-39 20.670782728384488 30.174132185985254 28.959464878968973 20.195620206661285 40-41 22.3174384694984 28.322795621196896 30.040748020775055 19.319017888529654 42-43 19.71169413733519 30.64637352517416 30.237407970372278 19.404524367118377 44-45 19.358913040679777 30.618955244424246 29.487960248073254 20.53417146682273 46-47 20.881801032811317 28.84157783466107 29.28985557475504 20.986765557772575 48-49 20.468588777476526 29.203445619302435 29.16165773145242 21.16630787176862 50-51 19.52294519157549 30.715998110634807 29.24376870922095 20.517287988568746 52-53 19.593757581458906 30.00559480722671 29.977868328935052 20.422779282379327 54-55 19.740812863699 29.110971486345242 30.36105875182391 20.78715689813185 56-57 20.219781852029726 29.62405866130621 28.3640882692241 21.792071217439954 58-59 20.364949770513878 30.09808241695672 28.055086570977313 21.481881241552088 60-61 19.97316472993915 28.414144464854214 30.510018665861278 21.10267213934536 62-63 20.546855272734472 28.379733924831534 30.255182623419962 20.818228179014024 64-65 20.800562847787994 29.344085270845742 28.947596434967267 20.907755446398998 66-67 20.639491417169623 30.78000524822625 27.943636030558537 20.636867304045587 68-69 21.311546836643856 28.657112216433745 30.03274708103392 19.998593865888473 70-71 22.135334921004294 28.60827932446416 29.158550895416713 20.097834859114833 72-73 21.176741445638775 29.617622157417077 28.87846395310264 20.327172443841505 74-75 20.22314863867943 30.070900565917224 30.074168329430172 19.631782465973174 76-77 20.609933010847982 28.686161021522675 30.38708144157882 20.31682452605051 78-79 20.211553972058518 28.89418885083633 29.6506794750364 21.243577702068748 80-81 20.750248795631098 29.52117362221683 29.389819431310126 20.338758150841944 82-83 20.53014594188863 29.375781053498823 30.28125847894844 19.812814525664106 84-85 20.26298603722186 28.995601880848255 30.46738494855988 20.274027133370005 86-87 19.286083858781357 30.687798465617504 28.83371581493337 21.19240186066777 88-89 29.261289257007228 18.316490695768135 35.820497449135644 16.60172259808899 90-91 30.7948289161253 14.966927163228029 16.965193801520016 37.27305011912666 92-93 22.548677536141586 17.734756234330064 36.091159371889894 23.625406857638456 94-95 15.888853482660098 7.096184004181913 27.82869909558966 49.186263417568334 96-97 27.07919616958946 7.251398674949678 14.745189310823303 50.92421584463756 98-99 57.221230180073576 14.090345158953838 18.6491971629985 10.039227497974082 100-101 30.591771499278202 7.26190967209734 12.402866570426891 49.74345225819757 102-103 23.628209973797865 2.1507408366846867 36.825252572438075 37.39579661707938 104-105 42.19148642520555 5.196671858672293 39.6906484749305 12.921193241191665 106-107 5.826175907554429 1.7462418480522583 6.309453171775534 86.11812907261778 108-109 17.946580182400844 11.046454330122646 21.94547685099702 49.06148863647949 110-111 40.31617832826964 2.7056462845038123 25.021296650190777 31.956878737035765 112-113 27.86616793681097 2.862825451805512 38.4622081544197 30.80879845696382 114-115 29.466269243220918 4.183365521655484 23.068793700763962 43.281571534359635 116-117 20.35953954846462 4.378659092854971 20.081131111773754 55.18067024690666 118-119 20.574232874187967 4.3559470924272325 15.061446996709826 60.008373036674975 120-121 26.31354761174314 4.372618271895418 29.0741337038455 40.239700412515944 122-123 35.03047502204818 1.690325858639684 33.7360869286837 29.54311219062844 124-125 38.95831465710444 1.9778873449873002 19.681059813735743 39.38273818417252 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 14.5 2 30.0 3 49.5 4 72.5 5 70.5 6 58.0 7 62.5 8 53.5 9 40.5 10 50.0 11 49.5 12 51.0 13 61.0 14 73.5 15 106.0 16 146.0 17 195.0 18 315.0 19 505.5 20 728.5 21 1044.0 22 1524.0 23 2294.5 24 3334.0 25 4647.5 26 6412.5 27 8731.0 28 11876.5 29 15763.0 30 20113.0 31 25270.5 32 31590.5 33 38275.5 34 44310.5 35 48864.5 36 51262.5 37 51386.0 38 50390.0 39 49247.5 40 47716.0 41 45909.0 42 43890.5 43 41689.0 44 39833.5 45 38232.0 46 36364.0 47 33990.5 48 31385.0 49 28747.5 50 25924.0 51 23098.5 52 20104.0 53 17124.0 54 14329.5 55 11750.0 56 9558.5 57 7559.5 58 5871.0 59 4572.5 60 3491.0 61 2631.5 62 1946.0 63 1377.5 64 985.0 65 750.5 66 527.0 67 366.0 68 275.0 69 197.5 70 145.0 71 102.5 72 78.5 73 70.5 74 53.0 75 41.5 76 30.0 77 18.5 78 10.5 79 6.5 80 5.0 81 2.5 82 1.5 83 2.0 84 3.5 85 4.0 86 2.0 87 0.5 88 1.0 89 1.0 90 1.5 91 2.5 92 1.5 93 0.5 94 1.0 95 1.5 96 1.5 97 3.0 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.931619572913211E-4 3 0.04178776371099107 4 0.04881840642066018 5 0.04901645269417199 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0011387660726928847 12-13 0.0020794858718739635 14-15 0.0 16-17 4.951156837795151E-4 18-19 0.00688210800453526 20-21 0.0025746015556534782 22-23 9.407197991810788E-4 24-25 0.0 26-27 0.0 28-29 0.0012377892094487877 30-31 0.0 32-33 0.0015843701880944483 34-35 1.4853470513385452E-4 36-37 2.9706941026770903E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.9804627351180604E-4 46-47 0.0 48-49 2.9706941026770903E-4 50-51 1.9804627351180604E-4 52-53 0.0 54-55 0.0015348586197164967 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.951156837795151E-5 66-67 0.0 68-69 3.960925470236121E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.456041154015636E-4 80-81 0.0 82-83 0.0010892545043149331 84-85 1.4853470513385452E-4 86-87 0.0012377892094487877 88-89 19.06145870982755 90-91 26.248013348318832 92-93 40.401043703861404 94-95 44.686071900699595 96-97 48.68928025033049 98-99 93.0959583706733 100-101 51.98368098706263 102-103 53.36465765226045 104-105 74.88822763438677 106-107 81.55906977665332 108-109 12.584256311487179 110-111 38.856381793606076 112-113 52.228713738965105 114-115 86.69470671822471 116-117 66.45977432627134 118-119 72.32615250553292 120-121 80.47219182762052 122-123 76.02813247315235 124-125 50.29286092695558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1009865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.38543988182712 #Duplication Level Percentage of deduplicated Percentage of total 1 86.12335522416771 66.64693728121071 2 9.914072993310914 15.344097992158154 3 2.2756780741864087 5.283130464010333 4 0.7397506257781633 2.289837103148002 5 0.3132250458047755 1.2119528975804001 6 0.15878543897418293 0.7372608625107696 7 0.10772374590578748 0.5835374623846276 8 0.062431906554702564 0.3865056441117427 9 0.04645797447448818 0.3235653711654267 >10 0.22689198719906103 3.1494147508189934 >50 0.01703657188118473 0.9046073015855811 >100 0.013054580349427193 1.995905392881535 >500 0.0011518735598847608 0.6372393886662432 >1k 3.839578532949202E-4 0.5060080877674599 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2088 0.2067603095463255 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1800 0.17824164616062543 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1221 0.12090724997895759 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 9.902313675590302E-5 0.0 4 0.0 0.0 0.0 9.902313675590302E-5 0.0 5 0.0 0.0 0.0 9.902313675590302E-5 0.0 6 0.0 0.0 0.0 9.902313675590302E-5 0.0 7 0.0 0.0 0.0 9.902313675590302E-5 0.0 8 0.0 0.0 0.0 9.902313675590302E-5 0.0 9 0.0 0.0 0.0 9.902313675590302E-5 0.0 10-11 0.0 0.0 0.0 9.902313675590302E-5 0.0 12-13 0.0 0.0 0.0 1.4853470513385454E-4 0.0 14-15 0.0 0.0 0.0 1.9804627351180604E-4 0.0 16-17 0.0 0.0 0.0 2.4755784188975754E-4 0.0 18-19 0.0 0.0 0.0 2.9706941026770903E-4 0.0 20-21 0.0 0.0 0.0 2.9706941026770903E-4 0.0 22-23 0.0 0.0 0.0 3.465809786456606E-4 0.0 24-25 0.0 0.0 0.0 5.446272521574666E-4 0.0 26-27 0.0 0.0 0.0 8.416966624251757E-4 0.0 28-29 0.0 0.0 0.0 0.0012377892094487877 0.0 30-31 0.0 0.0 0.0 0.0030697172394329934 0.0 32-33 0.0 0.0 0.0 0.007822827803716337 0.0 34-35 0.0 0.0 0.0 0.013417635030424858 0.0 36-37 0.0 0.0 0.0 0.023369460274393114 0.0 38-39 0.0 0.0 0.0 0.0427779950785501 0.0 40-41 0.0 0.0 0.0 0.06540478182727394 0.0 42-43 0.0 0.0 0.0 0.08619964054601358 0.0 44-45 0.0 0.0 0.0 0.10986617023067441 0.0 46-47 0.0 0.0 0.0 0.1392760418471776 0.0 48-49 0.0 0.0 0.0 0.17403316284849957 0.0 50-51 0.0 0.0 0.0 0.21339485970897099 0.0 52-53 0.0 0.0 0.0 0.25107316324459206 0.0 54-55 0.0 0.0 0.0 0.29464334341718945 0.0 56-57 0.0 0.0 0.0 0.3440063770900071 0.0 58-59 0.0 0.0 0.0 0.3995583568100687 0.0 60-61 0.0 0.0 0.0 0.4616458635560199 0.0 62-63 9.902313675590302E-5 0.0 0.0 0.5311105939902858 0.0 64-65 9.902313675590302E-5 0.0 0.0 0.5980502344372762 0.0 66-67 9.902313675590302E-5 0.0 0.0 0.6776153248206443 0.0 68-69 9.902313675590302E-5 0.0 0.0 0.7779752739227521 0.0 70-71 9.902313675590302E-5 0.0 0.0 0.8884850945423398 0.0 72-73 9.902313675590302E-5 0.0 0.0 0.9900828328538962 0.0 74-75 9.902313675590302E-5 0.0 0.0 1.0952949156570433 0.0 76-77 9.902313675590302E-5 0.0 0.0 1.2124887980076546 0.0 78-79 9.902313675590302E-5 0.0 0.0 1.2462556876414175 0.0 80-81 9.902313675590302E-5 0.0 0.0 1.2468498264619527 0.0 82-83 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 84-85 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 86-87 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 88-89 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 90-91 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 92-93 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 94-95 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 96-97 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 98-99 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 100-101 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 102-103 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 104-105 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 106-107 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 108-109 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 110-111 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 112-113 9.902313675590302E-5 0.0 0.0 1.2468993380303308 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGAC 15 3.6524762E-7 3896.9998 110-111 CCGCGCT 25 6.6211214E-10 3009.1619 108-109 CCGCTCC 205 0.0 2568.7966 108-109 CCGCGCC 180 0.0 1950.3827 108-109 CGCGCGA 15 0.0021496702 1948.4999 110-111 CCGCGCG 75 0.0 1671.7566 108-109 CGTCGTC 35 4.6376463E-6 1670.1428 110-111 CGCCGCT 35 4.6376463E-6 1670.1428 110-111 CTCCGCC 170 0.0 1547.3383 110-111 CCGCTCG 50 1.0586518E-8 1504.5809 108-109 CGTCGCG 20 0.0038212035 1461.3749 110-111 CGCGTCG 20 0.0038212035 1461.3749 110-111 CATCGCG 20 0.005193154 1253.8174 108-109 CGTCGAT 20 0.005193154 1253.8174 108-109 CGCGACG 25 0.0059699533 1169.1001 110-111 TTGCGCT 25 0.0059699533 1169.1001 110-111 CAGCGCG 45 1.562242E-5 1114.5044 108-109 CCGTTCG 45 1.562242E-5 1114.5044 108-109 CCCGTAC 25 0.00811323 1003.05396 108-109 CGTACGT 30 0.00859576 974.24994 110-111 >>END_MODULE