FastQCFastQC Report
Fri 27 May 2016
SRR939942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009865
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22280.22062354869215192No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18120.17942992380169628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13370.13239393384264234No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA11250.11140102885039091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10600.1049645249612572No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA10140.10040946067048566No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG558.489677E-443.3122525
TAGGACC16600.038.74624
GGGTGCG700.002789890433.9973568
GTATCAA36250.033.8466531
GTACCTG11250.032.8243141
TACCTGG11950.030.8984892
GTACATG37550.030.454241
TACATGG36050.030.231472
ATCAACG40250.029.8881443
TAGCACG800.00533802129.7771724
TCAACGC40650.029.7405474
CAACGCA41100.029.414925
GTCCTAC48800.028.8037261
GGTATCA26300.028.761031
AACGCAG42400.028.625136
GTAGGAC50000.028.5860863
ACATGGG36900.028.4053253
TACACGG1505.25609E-627.7920252
TATCAAC43850.027.5701982
TGTAGGA53600.027.4438862
Produced by FastQC (version 0.11.5)