FastQCFastQC Report
Fri 27 May 2016
SRR939941_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939941_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419547
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27530.656183931716828No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15470.36873103609369157No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9630.22953328232593728No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9330.22238271278307314No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8770.2090349829697269No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8390.199977594882099No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7990.19044350215828024No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5990.1427730385391863No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA5670.1351457643601313No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5380.12823354713536267No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5160.12298979613726234No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5120.12203638686488046No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA4700.11202558950487074No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4580.1091653616877251No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA4260.10153808750867005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGG109.215503E-42968.8308110-111
CGGCGCG201.4357211E-62460.4692108-109
ACGCTAC100.00134354782460.4692108-109
CCGCTCC750.02296.4377108-109
CTGTTCG352.6793714E-92108.9736108-109
CCGCGCC503.6379788E-121968.3752108-109
CAGCGCG150.0030225791640.3129108-109
CCGCGCT150.0030225791640.3129108-109
CCGCACC601.0913936E-111640.3129108-109
TGCGTAG200.00368538731484.4154110-111
CCTCGCG357.691127E-61405.9824108-109
CCGCGCG200.00537275571230.2346108-109
CCGCCGC200.00537275571230.2346108-109
CGTCTTG501.2707058E-51187.5322110-111
CGCCGTG250.00575778261187.5322110-111
CTGCTCG451.6341572E-51093.5419108-109
CCGCTCG451.6341572E-51093.5419108-109
CATCGGC451.6341572E-51093.5419108-109
TTGCTCG300.008290291989.6102110-111
TTCGTGC300.008290291989.6102110-111
Produced by FastQC (version 0.11.5)