##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939941_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419547 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53859996615397 33.0 31.0 34.0 29.0 34.0 2 31.551170667410325 34.0 31.0 34.0 28.0 34.0 3 31.731028943121988 34.0 31.0 34.0 29.0 34.0 4 34.973721656929975 37.0 35.0 37.0 32.0 37.0 5 35.05854409637061 37.0 35.0 37.0 32.0 37.0 6 35.087923403099055 37.0 35.0 37.0 32.0 37.0 7 35.04325856221115 37.0 36.0 37.0 32.0 37.0 8 35.06865500170422 37.0 36.0 37.0 33.0 37.0 9 36.6747968642369 39.0 38.0 39.0 33.0 39.0 10-11 36.63692625617631 39.0 38.0 39.0 33.0 39.0 12-13 36.57171663723015 39.0 38.0 39.0 33.0 39.0 14-15 38.02787292007808 41.0 39.0 41.0 33.0 41.0 16-17 38.031959470571834 41.0 39.0 41.0 33.0 41.0 18-19 38.03274484145996 41.0 39.0 41.0 33.0 41.0 20-21 38.10007460427556 41.0 39.0 41.0 33.0 41.0 22-23 38.074641220173184 41.0 39.0 41.0 33.0 41.0 24-25 38.09496313881401 41.0 39.0 41.0 33.0 41.0 26-27 38.05212646020588 41.0 39.0 41.0 33.0 41.0 28-29 38.00072697457019 40.0 38.5 41.0 33.0 41.0 30-31 37.90829513737436 40.0 38.0 41.0 33.0 41.0 32-33 37.8030506713193 40.0 38.0 41.0 32.0 41.0 34-35 37.729037509504295 40.0 38.0 41.0 32.0 41.0 36-37 37.88839629409816 40.0 38.0 41.0 33.0 41.0 38-39 37.81871756918772 40.0 38.0 41.0 32.0 41.0 40-41 37.72920912317333 40.0 38.0 41.0 32.0 41.0 42-43 37.66072096809178 40.0 38.0 41.0 32.0 41.0 44-45 37.55972513210677 40.0 38.0 41.0 31.5 41.0 46-47 37.418085458840125 40.0 38.0 41.0 31.0 41.0 48-49 37.30822649190675 40.0 38.0 41.0 31.0 41.0 50-51 37.18206184289245 40.0 38.0 41.0 30.0 41.0 52-53 37.094864222602 40.0 37.5 41.0 30.5 41.0 54-55 36.930256919963675 40.0 37.0 41.0 30.0 41.0 56-57 36.67689674815932 40.0 37.0 41.0 30.0 41.0 58-59 36.49086991445535 40.0 36.0 41.0 30.0 41.0 60-61 36.23334930293865 40.0 36.0 41.0 29.0 41.0 62-63 36.004279615871404 39.0 35.5 41.0 29.0 41.0 64-65 35.71606875987672 39.0 35.0 41.0 28.0 41.0 66-67 35.36183312000801 39.0 35.0 41.0 28.0 41.0 68-69 34.88788622013743 38.0 34.5 40.0 27.0 41.0 70-71 34.42177396096265 37.0 34.0 40.0 26.0 41.0 72-73 34.026513120103345 37.0 34.0 39.0 26.0 41.0 74-75 33.60431608377607 36.0 34.0 39.0 26.0 41.0 76-77 33.1059773994332 36.0 33.5 39.0 24.5 40.0 78-79 32.58649448095207 35.5 33.0 37.5 22.0 39.5 80-81 32.0642645520049 35.0 32.5 37.0 19.0 39.0 82-83 31.809875889947968 35.0 33.0 37.0 15.5 39.0 84-85 31.321229802620444 35.0 33.0 36.5 2.0 38.0 86-87 30.57917110597859 35.0 33.0 36.0 2.0 37.0 88-89 15.293101845562 18.0 16.0 18.5 2.0 19.0 90-91 2.7169458964073154 2.0 2.0 2.0 2.0 2.0 92-93 2.2742338760615617 2.0 2.0 2.0 2.0 2.0 94-95 2.0914688938307266 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25728262849681016 1101 2 0.21806795675889035 1101 3 0.3467150548187199 1101 4 0.2606860182431987 1101 5 0.23718344718464834 1101 6 0.09093387981180712 1101 7 0.29310787273608696 1101 8 0.3122627366849926 1101 9 0.40866717462280633 1101 10-11 0.27829969631227414 1101 12-13 0.33941253303234475 1101 14-15 0.4549052834800662 1101 16-17 0.5427876784312602 1101 18-19 0.46683135533601217 1101 20-21 0.6262684978732906 1101 22-23 0.4743560074395816 1101 24-25 0.35840753137636483 1101 26-27 0.43900301399816044 1101 28-29 0.3667174774730526 1101 30-31 0.42231221410452946 1101 32-33 0.4273353597742897 1101 34-35 0.5669984251344147 1101 36-37 0.5961192286845858 1101 38-39 0.5340664428371582 1101 40-41 0.406921480472171 1101 42-43 0.4785748046664722 1101 44-45 0.5503677919017704 1101 46-47 0.539442844714614 1101 48-49 0.6070443392261637 1101 50-51 0.6110922903060896 1101 52-53 0.48481621848582535 1101 54-55 0.44980736262863275 1101 56-57 0.5702198176805453 1101 58-59 0.3802753121157565 1101 60-61 0.4828531815457566 1101 62-63 0.2932124965992031 1101 64-65 0.3946582682592137 1101 66-67 0.5771263778554427 1101 68-69 0.6346069541998816 1101 70-71 0.3930107392538602 1101 72-73 0.7468983605298476 1101 74-75 0.7587101449375595 1101 76-77 0.7090972552036234 1101 78-79 0.6661698852136766 1101 80-81 0.6558018687462663 1101 82-83 0.8198118641783871 1101 84-85 0.9126299750339157 1101 86-87 0.9563052579253792 1101 88-89 0.9175452458140931 1101 90-91 -0.11977393023195759 1101 92-93 -0.2837334220311871 1101 94-95 -0.0982726193078376 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.17784936145848107 1102 2 -0.15076587676839637 1102 3 -0.0052632633046378885 1102 4 -0.01520361242992152 1102 5 -0.0071449658734792365 1102 6 -0.23872864239068292 1102 7 -0.09024485587296738 1102 8 -0.30749900766485183 1102 9 -0.12277767133787165 1102 10-11 -0.06771151211547988 1102 12-13 -0.23962066351325007 1102 14-15 -0.25481481206718115 1102 16-17 -0.31083668611825743 1102 18-19 -0.4511679709333549 1102 20-21 -0.3406099856203113 1102 22-23 -0.14740089829938086 1102 24-25 -0.197369708777984 1102 26-27 -0.20399631227121517 1102 28-29 -0.28737084248774636 1102 30-31 -0.1529404348818062 1102 32-33 -0.345603636979547 1102 34-35 -0.33404126372452936 1102 36-37 -0.3646451493395375 1102 38-39 -0.4879691423897654 1102 40-41 -0.5213553003203728 1102 42-43 -0.36884970687882657 1102 44-45 -0.5204397661309415 1102 46-47 -0.6956707707689418 1102 48-49 -0.7477437418665005 1102 50-51 -0.6145278481149319 1102 52-53 -0.39848291791400214 1102 54-55 -0.656447613790796 1102 56-57 -0.5550680490432853 1102 58-59 -0.24912843627647163 1102 60-61 -0.4244644518222813 1102 62-63 -0.5320661828887623 1102 64-65 -0.43633487193800846 1102 66-67 -0.4089534286001282 1102 68-69 -0.6171631199104866 1102 70-71 -0.3907814454708287 1102 72-73 -0.4807092832503983 1102 74-75 -0.39417464712467165 1102 76-77 -0.4614515290188308 1102 78-79 -0.34931673204608416 1102 80-81 -0.3539947870998752 1102 82-83 -0.39879994448211065 1102 84-85 -0.30479821140572483 1102 86-87 -0.14619673264239097 1102 88-89 0.4167597371474656 1102 90-91 0.10821192142480696 1102 92-93 -1.1273237601461261E-4 1102 94-95 0.1086239324163003 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.14152284612543653 1103 2 0.25021361687348787 1103 3 0.19782414647884394 1103 4 0.3450495666283899 1103 5 0.33052830712457393 1103 6 0.18552384930367083 1103 7 0.1548003955876851 1103 8 0.18630898866920376 1103 9 0.3175705930681261 1103 10-11 0.18363743965447554 1103 12-13 0.2826184245838945 1103 14-15 0.19299707932930943 1103 16-17 0.284434822645629 1103 18-19 0.37403823825440696 1103 20-21 0.5428904153419936 1103 22-23 0.611531969153944 1103 24-25 0.6441041141112365 1103 26-27 0.6659773387770187 1103 28-29 0.6381189043331972 1103 30-31 0.7047809535933922 1103 32-33 0.6761256561811777 1103 34-35 0.583265987965234 1103 36-37 0.5414494777655108 1103 38-39 0.5848828452376864 1103 40-41 0.6502651602134719 1103 42-43 0.589455940961706 1103 44-45 0.5358410220464052 1103 46-47 0.623254650241492 1103 48-49 0.5427677714798165 1103 50-51 0.5042887402686205 1103 52-53 0.44160472099079584 1103 54-55 0.47853634771682607 1103 56-57 0.4148053614275611 1103 58-59 0.23843314007820737 1103 60-61 0.3822058425986157 1103 62-63 0.510009083464773 1103 64-65 0.6235946732561715 1103 66-67 0.6872861177396601 1103 68-69 0.6672394462482956 1103 70-71 0.7044843352600694 1103 72-73 0.7369330583694875 1103 74-75 0.630289781790438 1103 76-77 0.8028227292008978 1103 78-79 0.41130304112381566 1103 80-81 0.21544615687996327 1103 82-83 0.26123270530594667 1103 84-85 0.25768317248704875 1103 86-87 0.5199563203991957 1103 88-89 1.0370226538618645 1103 90-91 -0.5390586708538043 1103 92-93 -0.2837334220311871 1103 94-95 -0.0982726193078376 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.028053619254553297 1104 2 0.12056667729355652 1104 3 0.14871102073978193 1104 4 0.36173969557709285 1104 5 0.20246929479279885 1104 6 0.12398322201610057 1104 7 0.03973761052095881 1104 8 0.04063074102012365 1104 9 0.07991081492746588 1104 10-11 0.06488638385178547 1104 12-13 0.19473059306184837 1104 14-15 0.11266034865754193 1104 16-17 0.10931234953340407 1104 18-19 0.027163414221519133 1104 20-21 0.012677565519375378 1104 22-23 -0.1408696300351977 1104 24-25 -0.02289571696032766 1104 26-27 -0.03880052033666459 1104 28-29 -0.11568761630422841 1104 30-31 -0.10305732558899905 1104 32-33 -0.11934706187727073 1104 34-35 -0.03487411001325569 1104 36-37 -0.19386782613673859 1104 38-39 0.007837403489837413 1104 40-41 0.19367976689178334 1104 42-43 0.06928588634151112 1104 44-45 -0.11197980119815298 1104 46-47 -0.09105358783497763 1104 48-49 -0.13034163783377295 1104 50-51 -0.18892878324059126 1104 52-53 -0.1738043673587768 1104 54-55 -0.03922084581884633 1104 56-57 -0.14692953355526583 1104 58-59 -0.37924240471598125 1104 60-61 -0.33824294393215837 1104 62-63 -0.23897734595129805 1104 64-65 -0.25183750197891897 1104 66-67 -0.21793940171526316 1104 68-69 -0.34781770787074606 1104 70-71 -0.5191712759793106 1104 72-73 -0.5106625269675291 1104 74-75 -0.5490840568266009 1104 76-77 -0.4229360410001277 1104 78-79 -0.46758090504432914 1104 80-81 -0.5986762599227866 1104 82-83 -0.7397555260133792 1104 84-85 -0.8335241026973677 1104 86-87 -0.7513399237586995 1104 88-89 0.2145242023724805 1104 90-91 -0.567255641403948 1104 92-93 -0.2837334220311871 1104 94-95 -0.0982726193078376 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.21679160561856392 1105 2 0.08467226038273878 1105 3 0.12574615121265964 1105 4 0.1843869396284603 1105 5 0.2718896282995473 1105 6 0.2229837970277373 1105 7 0.14188705878360253 1105 8 0.07432094665524147 1105 9 0.16136805870502258 1105 10-11 0.0833511027806253 1105 12-13 0.16812124501552717 1105 14-15 0.1999676513053288 1105 16-17 0.2332068212072329 1105 18-19 0.14728197614479654 1105 20-21 0.258767486386823 1105 22-23 0.14876783881832978 1105 24-25 0.21565881330992198 1105 26-27 0.18568949910419974 1105 28-29 0.15187488198442622 1105 30-31 -0.06654134847993731 1105 32-33 -0.05898589980613167 1105 34-35 0.007531629150513197 1105 36-37 -0.04548744224801737 1105 38-39 -0.12052025374846664 1105 40-41 -0.03477098746872542 1105 42-43 -0.1866203046171151 1105 44-45 -0.058377764014068134 1105 46-47 -0.1130792716881075 1105 48-49 -0.0011488446462948332 1105 50-51 -0.10967575073873803 1105 52-53 0.16037870539388877 1105 54-55 0.00500439445387002 1105 56-57 0.07873925974174512 1105 58-59 0.044341286858724516 1105 60-61 0.04531856862226391 1105 62-63 0.2517745502180233 1105 64-65 -0.07974610251012137 1105 66-67 -0.34308383916220464 1105 68-69 0.026558678318025386 1105 70-71 -0.18549065387147579 1105 72-73 -0.17476811005671067 1105 74-75 -0.4238136644496109 1105 76-77 -0.41932852751473604 1105 78-79 -0.26422119419304124 1105 80-81 -0.22105790336080844 1105 82-83 -0.17705324513388732 1105 84-85 -0.4724852483395985 1105 86-87 -0.38235906343176396 1105 88-89 0.2826014729839095 1105 90-91 -0.6943742854717447 1105 92-93 -0.2837334220311871 1105 94-95 -0.0982726193078376 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.06634971222206332 1106 2 -0.1274347019802029 1106 3 0.049172546959244556 1106 4 -0.15238725065775327 1106 5 -0.06650765542072179 1106 6 0.10211642803185583 1106 7 0.00532473022600044 1106 8 0.0057085904140379284 1106 9 0.018159137253135782 1106 10-11 -0.366657872268739 1106 12-13 -0.16383549271836984 1106 14-15 0.0012587943221120668 1106 16-17 -0.022826055283445612 1106 18-19 -0.191588077781347 1106 20-21 -0.026221933167668965 1106 22-23 -0.14030333205901968 1106 24-25 -0.12061858903269496 1106 26-27 -0.24054317968257521 1106 28-29 -0.056573799694589866 1106 30-31 -0.13597157189546039 1106 32-33 0.007916140690298334 1106 34-35 -0.025823952192510546 1106 36-37 0.06739845327229688 1106 38-39 0.06446056998409233 1106 40-41 0.06540067615147649 1106 42-43 -0.40202654568945917 1106 44-45 -0.0705193624829974 1106 46-47 -0.2088594815778464 1106 48-49 -0.2130383832781888 1106 50-51 -0.39197644775877194 1106 52-53 -0.43673638507713264 1106 54-55 -0.38373027527695314 1106 56-57 -0.6459441485522177 1106 58-59 -0.3404848120791826 1106 60-61 -0.3148602750374394 1106 62-63 -0.21353372376621138 1106 64-65 -0.271334332301727 1106 66-67 -0.2944466561647445 1106 68-69 -0.4109168299099437 1106 70-71 -0.3499754986789654 1106 72-73 -0.45718383939955487 1106 74-75 -0.5195888179735135 1106 76-77 -0.35108781416263923 1106 78-79 -0.2591408106171258 1106 80-81 -0.4031907290350105 1106 82-83 -0.1281683002103513 1106 84-85 -0.11496226083640693 1106 86-87 -0.23970760631353727 1106 88-89 0.050277895908461545 1106 90-91 -0.24836489580038323 1106 92-93 -0.24147990090442617 1106 94-95 -0.0982726193078376 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.041631508435212794 1107 2 0.038449492073002745 1107 3 0.10394562990134304 1107 4 0.15121212336415368 1107 5 0.16979907384374826 1107 6 0.05829796167817847 1107 7 0.07105242975652004 1107 8 0.20289168900558963 1107 9 0.21064740016393557 1107 10-11 0.2898366504229699 1107 12-13 0.10768250415173952 1107 14-15 0.23208821529237866 1107 16-17 0.2690362295365887 1107 18-19 0.29432741517640437 1107 20-21 0.25859809813123746 1107 22-23 0.34013485260452114 1107 24-25 0.12586028420674467 1107 26-27 0.11704680466797868 1107 28-29 0.28771415022716695 1107 30-31 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-0.013819863282691358 2216 36-37 -0.03711177118182718 2216 38-39 -0.09165034496396629 2216 40-41 -0.0434785353602436 2216 42-43 -0.01912825841085919 2216 44-45 0.019227412814409206 2216 46-47 0.08022491648492291 2216 48-49 0.09680769411467338 2216 50-51 -0.09902981718610704 2216 52-53 -0.12308910367905668 2216 54-55 -0.1803189998247987 2216 56-57 -1.0454889920197274E-4 2216 58-59 0.03598586590391051 2216 60-61 -0.1399024500933379 2216 62-63 -0.30166324368983766 2216 64-65 -0.30321845660807156 2216 66-67 -0.15096428250464555 2216 68-69 -0.39862503519239567 2216 70-71 -0.45533576301957623 2216 72-73 -0.30003979905099953 2216 74-75 -0.35249277218470354 2216 76-77 -0.19753355764240155 2216 78-79 -0.2696605991097698 2216 80-81 -0.3952156112282026 2216 82-83 -0.46698983978515685 2216 84-85 -0.47650602045384716 2216 86-87 -0.6080015544654636 2216 88-89 -1.021961327111491 2216 90-91 -0.6943742854717447 2216 92-93 -0.2837334220311871 2216 94-95 -0.0982726193078376 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6745.0 3 1046.0 4 700.0 5 595.0 6 530.0 7 650.0 8 738.0 9 916.0 10 944.0 11 1187.0 12 1413.0 13 1701.0 14 1920.0 15 2204.0 16 2530.0 17 2993.0 18 3937.0 19 4734.0 20 4929.0 21 6899.0 22 8105.0 23 10376.0 24 15544.0 25 26058.0 26 44821.0 27 111804.0 28 150167.0 29 5177.0 30 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5376584744975 20.236588510941562 14.559751350861763 23.66600166369918 2 12.725305213780741 20.29808696149611 45.59019120850832 21.386416616214824 3 20.138585861476134 25.73972468816989 29.086101116174152 25.035588334179828 4 11.082920408426054 17.325438876780662 39.87328491103237 31.718355803760918 5 11.497914694429408 39.01846371759073 34.52736650586723 14.956255082112632 6 28.466655702460038 36.66216180785466 20.8713207340298 13.999861755655504 7 28.038336586842476 31.887249819448122 22.167242287514867 17.907171306194538 8 24.167018236335856 38.679814180532816 20.34980586203691 16.80336172109442 9 25.367837214900835 19.368986073074055 22.825094685458364 32.43808202656675 10-11 23.977790198655924 26.179106149645627 30.471499447615212 19.371604204083237 12-13 27.10718882585689 24.205081756209182 29.1489488487391 19.538780569194834 14-15 20.394258569361718 30.463809775781975 25.918073541224224 23.223858113632083 16-17 19.603667293136755 30.750584261816034 30.625925850888997 19.019822594158217 18-19 20.914502970674643 28.862800717490927 31.044235341791467 19.178460970042966 20-21 21.859018898146417 28.723108844599484 28.174291513174595 21.24358074407951 22-23 20.108903563499073 31.362629321491948 28.718100897280507 19.810366217728472 24-25 21.582802403544775 28.979113186365296 30.573094313628747 18.864990096461185 26-27 19.25290849416156 30.626366056723086 30.370852371724745 19.749873077390614 28-29 18.877438530964234 31.776550774001556 27.694346552954958 21.65166414207925 30-31 20.24135555730347 28.941572696265254 29.50908956565057 21.307982180780698 32-33 19.310008664220348 30.278291897225902 30.873703793307694 19.53799564524606 34-35 20.049839469713763 30.461187900282926 30.356074528002825 19.132898102000492 36-37 19.10441499998808 29.667117152547867 30.29982338093229 20.92864446653176 38-39 20.391875046180765 31.090318843895915 28.739926635156486 19.77787947476683 40-41 22.501531413643765 28.290394163228434 30.29040846436752 18.91766595876028 42-43 19.038748936352782 31.326406814969477 30.048242509182522 19.58660173949522 44-45 19.06663615756995 30.892009715240487 29.062178969221563 20.979175157968 46-47 21.534059354494254 28.963262757211943 29.166696460706426 20.335981427587374 48-49 20.40665288990268 29.721223128755536 29.007834640695794 20.864289340645982 50-51 18.304981325095877 31.48169335020868 29.971373886596737 20.241951438098713 52-53 18.785142069899198 30.364297682977114 30.164915968890256 20.68564427823343 54-55 19.141968077055548 29.037294284355657 30.426072271753807 21.394665366834985 56-57 20.33240614281594 29.584885602804928 27.747070054129814 22.335638200249317 58-59 20.856423714148832 30.42984457045337 27.261665558328385 21.45206615706941 60-61 19.668475760761012 28.042507752409147 31.866751520091906 20.422264966737934 62-63 20.091193596903327 28.28646134997986 30.69167459188124 20.93067046123557 64-65 20.71873014965004 30.2669197977335 28.064178974628497 20.950171077987967 66-67 20.681473112666758 31.356439207049508 27.191232448331178 20.77085523195256 68-69 21.460945050989945 28.366426842508584 30.19067152750549 19.98195657899598 70-71 22.77885433574784 28.22544315654742 28.94681644726336 20.048886060441383 72-73 21.118253735576705 30.086021351600657 28.77746712525653 20.018257787566114 74-75 19.949254791477475 31.108433620071175 29.94300996074337 18.99930162770798 76-77 20.661689870264834 28.70667648678217 30.546756382479195 20.084877260473796 78-79 20.20241023587514 28.73441164693095 29.798900711248404 21.264277405945503 80-81 21.03530712887948 29.659728230686905 29.454506884806708 19.850457755626902 82-83 20.286191674770706 29.214575825181626 31.156684400205936 19.342548099841732 84-85 19.88313571916343 29.561800658568238 30.752848611536503 19.802215010731825 86-87 18.498668783265522 31.99258236978031 28.094751168538952 21.41399767841522 88-89 28.963475847407583 19.13915790716901 35.55214361639305 16.345222629030353 90-91 30.670995705976562 14.971548256451944 17.203082905196947 37.154373132374545 92-93 22.658800377549817 17.42861322586657 36.48606131777711 23.42652507880651 94-95 16.56089407233504 7.156040722268503 28.31192998020145 47.97113522519501 96-97 28.315429653506357 7.5747765811049454 15.12495445758696 48.984839307801735 98-99 57.19535345443146 13.976209965342482 18.98500496351382 9.843431616712238 100-101 30.94638198968932 7.4155303077132295 12.552573343058834 49.08551435953862 102-103 23.931840261798847 2.2692642020759832 36.94712890525131 36.851766630873854 104-105 42.29322413218418 5.164039807628209 40.34522165611161 12.197514404075996 106-107 6.466559357710279 2.024098898019563 7.112368117509823 84.39697362676033 108-109 18.14115829526707 10.979296401903472 22.662790333325614 48.216754969503846 110-111 40.62690764697049 2.8533035325965064 25.601134300297034 30.918654520135973 112-113 28.46397535530353 3.038896780362131 38.83037548711471 29.666752377219634 114-115 30.477722257863398 4.412176631637918 23.47950230864454 41.63059880185415 116-117 21.278481282965682 4.496145550833885 20.55777402891371 53.66759913728673 118-119 21.97407385282346 4.661244762286758 15.632067179272738 57.73261420561705 120-121 27.008588807042212 4.591496775376715 29.788183513158295 38.61173090442278 122-123 35.46647878597091 1.8168463025978503 33.91746241136878 28.79921250006246 124-125 39.62925660388166 2.1923836981737295 19.99207629667109 38.18628340127352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 14.5 2 27.0 3 45.0 4 61.0 5 62.5 6 54.0 7 41.0 8 37.0 9 39.5 10 41.5 11 35.0 12 36.5 13 46.5 14 49.0 15 64.0 16 79.0 17 109.0 18 174.0 19 243.0 20 347.5 21 497.0 22 678.5 23 966.0 24 1345.0 25 1842.5 26 2524.0 27 3408.0 28 4500.5 29 5826.5 30 7830.5 31 10547.0 32 13758.0 33 17341.5 34 20718.0 35 23169.0 36 23988.5 37 23311.5 38 22166.5 39 20919.5 40 19576.0 41 18006.0 42 16448.0 43 15685.5 44 15367.5 45 14576.5 46 13373.5 47 12410.0 48 11696.5 49 11205.5 50 10559.0 51 9536.0 52 8180.5 53 6760.5 54 5757.5 55 4861.5 56 3976.0 57 3257.0 58 2614.5 59 2086.0 60 1668.0 61 1301.5 62 996.5 63 702.5 64 510.5 65 378.0 66 263.5 67 203.5 68 158.5 69 120.0 70 92.5 71 60.5 72 37.0 73 34.5 74 33.5 75 23.0 76 14.5 77 11.0 78 6.0 79 6.5 80 6.0 81 2.0 82 2.5 83 4.0 84 3.0 85 1.0 86 3.0 87 3.5 88 1.5 89 2.5 90 1.5 91 1.5 92 1.5 93 0.5 94 1.5 95 1.5 96 2.0 97 2.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0011917615904773482 3 0.0400431894400389 4 0.04314176957528001 5 0.04385682652956641 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.958807952386741E-4 12-13 0.0016684662266682874 14-15 0.0 16-17 1.1917615904773482E-4 18-19 0.007031393383816354 20-21 0.002025994703811492 22-23 3.575284771432045E-4 24-25 0.0 26-27 0.0 28-29 0.0015492900676205526 30-31 0.0 32-33 0.0013109377495250831 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0013109377495250831 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.575284771432045E-4 66-67 0.0 68-69 8.342331133341437E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 7.15056954286409E-4 80-81 0.0 82-83 7.15056954286409E-4 84-85 1.1917615904773482E-4 86-87 0.001430113908572818 88-89 19.274836907426344 90-91 26.257725594510266 92-93 40.53026240206699 94-95 44.922142215294116 96-97 48.644967071627256 98-99 93.15702412363811 100-101 51.800990115529366 102-103 53.385437150069 104-105 74.97181483838521 106-107 81.71229921796605 108-109 12.521481502668355 110-111 38.8937353860235 112-113 52.26124844177173 114-115 86.81065530202814 116-117 66.51459788772176 118-119 72.07011371789096 120-121 80.5045680221763 122-123 76.14963281825398 124-125 50.065666063635305 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 419547.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.71433880891774 #Duplication Level Percentage of deduplicated Percentage of total 1 92.02129969375514 73.35417061424955 2 5.682643571295165 9.05976349945082 3 1.0169269383364379 2.431909755193986 4 0.4086072033130915 1.3028741217865645 5 0.21164922744116993 0.8435739112445554 6 0.1377724741800529 0.6589465011198965 7 0.09234306611208824 0.5152746521099306 8 0.05911558142588907 0.3769887589335589 9 0.05276401618890226 0.37854437970612376 >10 0.2696650919580644 4.049855514269162 >50 0.023905385435102115 1.3077193653710477 >100 0.01972194278065657 2.9946535025931094 >500 0.0029881731485843274 1.701464362792123 >1k 5.976346297168654E-4 1.0242610611796348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2753 0.656183931716828 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1547 0.36873103609369157 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 963 0.22953328232593728 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 933 0.22238271278307314 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 877 0.2090349829697269 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 839 0.199977594882099 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 799 0.19044350215828024 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 599 0.1427730385391863 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 567 0.1351457643601313 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 538 0.12823354713536267 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 516 0.12298979613726234 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 512 0.12203638686488046 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 470 0.11202558950487074 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 458 0.1091653616877251 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 426 0.10153808750867005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3835231809546964E-4 0.0 5 0.0 0.0 0.0 2.3835231809546964E-4 0.0 6 0.0 0.0 0.0 2.3835231809546964E-4 0.0 7 0.0 0.0 0.0 2.3835231809546964E-4 0.0 8 0.0 0.0 0.0 2.3835231809546964E-4 0.0 9 0.0 0.0 0.0 2.3835231809546964E-4 0.0 10-11 0.0 0.0 0.0 2.3835231809546964E-4 0.0 12-13 0.0 0.0 0.0 2.3835231809546964E-4 0.0 14-15 0.0 0.0 0.0 2.3835231809546964E-4 0.0 16-17 0.0 0.0 0.0 2.3835231809546964E-4 0.0 18-19 0.0 0.0 0.0 2.3835231809546964E-4 0.0 20-21 0.0 0.0 0.0 4.767046361909393E-4 0.0 22-23 0.0 0.0 0.0 4.767046361909393E-4 0.0 24-25 0.0 0.0 0.0 5.958807952386741E-4 0.0 26-27 0.0 0.0 0.0 7.15056954286409E-4 0.0 28-29 0.0 0.0 0.0 0.0010725854314296134 0.0 30-31 0.0 0.0 0.0 0.002741051658097901 0.0 32-33 0.0 0.0 0.0 0.005124574839052597 0.0 34-35 0.0 0.0 0.0 0.010129973519057459 0.0 36-37 0.0 0.0 0.0 0.019425713924780777 0.0 38-39 0.0 0.0 0.0 0.035276143078129506 0.0 40-41 0.0 0.0 0.0 0.05517856163910122 0.0 42-43 0.0 0.0 0.0 0.07853708881245725 0.0 44-45 0.0 0.0 0.0 0.10415996300772024 0.0 46-47 0.0 0.0 0.0 0.13431153124679712 0.0 48-49 0.0 0.0 0.0 0.1647014518039695 0.0 50-51 0.0 0.0 0.0 0.19675983858781018 0.0 52-53 0.0 0.0 0.0 0.23430032868784667 0.0 54-55 0.0 0.0 0.0 0.2739859896507424 0.0 56-57 0.0 0.0 0.0 0.32654267579079344 0.0 58-59 0.0 0.0 0.0 0.3874416930641859 0.0 60-61 0.0 0.0 0.0 0.4569213937890153 0.0 62-63 0.0 0.0 0.0 0.5300955554443245 0.0 64-65 0.0 0.0 0.0 0.6033888932586814 0.0 66-67 0.0 0.0 0.0 0.686216323796857 0.0 68-69 0.0 0.0 0.0 0.7888269967369568 0.0 70-71 0.0 0.0 0.0 0.9018059955142094 0.0 72-73 0.0 0.0 0.0 1.0187178075400372 0.0 74-75 0.0 0.0 0.0 1.1237120036610917 0.0 76-77 0.0 0.0 0.0 1.2419347534364444 0.0 78-79 0.0 0.0 0.0 1.2778067773098127 0.0 80-81 0.0 0.0 0.0 1.2785218342640992 0.0 82-83 0.0 0.0 0.0 1.2785218342640992 0.0 84-85 0.0 0.0 0.0 1.2785218342640992 0.0 86-87 0.0 0.0 0.0 1.2785218342640992 0.0 88-89 0.0 0.0 0.0 1.2785218342640992 0.0 90-91 0.0 0.0 0.0 1.2785218342640992 0.0 92-93 0.0 0.0 0.0 1.2785218342640992 0.0 94-95 0.0 0.0 0.0 1.2785218342640992 0.0 96-97 0.0 0.0 0.0 1.2785218342640992 0.0 98-99 0.0 0.0 0.0 1.2785218342640992 0.0 100-101 0.0 0.0 0.0 1.2785218342640992 0.0 102-103 0.0 0.0 0.0 1.2785218342640992 0.0 104-105 0.0 0.0 0.0 1.2785218342640992 0.0 106-107 0.0 0.0 0.0 1.2785218342640992 0.0 108-109 0.0 0.0 0.0 1.2785218342640992 0.0 110-111 0.0 0.0 0.0 1.2785218342640992 0.0 112-113 0.0 0.0 0.0 1.2785218342640992 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTCGG 10 9.215503E-4 2968.8308 110-111 CGGCGCG 20 1.4357211E-6 2460.4692 108-109 ACGCTAC 10 0.0013435478 2460.4692 108-109 CCGCTCC 75 0.0 2296.4377 108-109 CTGTTCG 35 2.6793714E-9 2108.9736 108-109 CCGCGCC 50 3.6379788E-12 1968.3752 108-109 CAGCGCG 15 0.003022579 1640.3129 108-109 CCGCGCT 15 0.003022579 1640.3129 108-109 CCGCACC 60 1.0913936E-11 1640.3129 108-109 TGCGTAG 20 0.0036853873 1484.4154 110-111 CCTCGCG 35 7.691127E-6 1405.9824 108-109 CCGCGCG 20 0.0053727557 1230.2346 108-109 CCGCCGC 20 0.0053727557 1230.2346 108-109 CGTCTTG 50 1.2707058E-5 1187.5322 110-111 CGCCGTG 25 0.0057577826 1187.5322 110-111 CTGCTCG 45 1.6341572E-5 1093.5419 108-109 CCGCTCG 45 1.6341572E-5 1093.5419 108-109 CATCGGC 45 1.6341572E-5 1093.5419 108-109 TTGCTCG 30 0.008290291 989.6102 110-111 TTCGTGC 30 0.008290291 989.6102 110-111 >>END_MODULE