FastQCFastQC Report
Fri 27 May 2016
SRR939941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419547
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31750.7567686099531161No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14280.3403671102403306No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9920.2364454995507059No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9340.22262106510116866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.21165685846877705No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8840.21070344919639517No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8650.20617475515258124No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8220.19592560547447602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.164939804122065No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA6300.15016196040014587No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC6090.14515656172014102No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5870.13991281072204068No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5580.13300059349727206No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5360.12775684249917174No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5100.12155968222868953No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA5050.12036792063821217No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA4680.1115488848686798No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4550.10845030473343867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTG401.9810104E-674.366066
TACGACG453.961977E-666.174164
ACGACGT507.401819E-659.5567365
TACCTGG5500.057.3910372
GTACACG453.1500115E-452.9519581
GACGGAC350.00776351151.0486343
ACCTGGG6400.048.3898473
GTACCTG6550.048.2024461
TACACGG505.303721E-447.645392
TGTACGA600.001301448939.704492
GTACGAC650.001928451536.65033
TACATGG22600.035.0488742
GTACATG22900.034.8581121
ACATGGG21200.034.5541463
CATGGGT4500.033.0870744
TAGGACC8600.031.1634084
CCTGGGG3550.030.197784
CTGTACG800.005327235429.7854751
TGGATAG1403.0653773E-629.7783685
CATGGGG10550.028.790464
Produced by FastQC (version 0.11.5)