##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 419547 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80223193110665 34.0 31.0 34.0 31.0 34.0 2 32.86892767675612 34.0 31.0 34.0 31.0 34.0 3 32.9183643310523 34.0 31.0 34.0 31.0 34.0 4 36.325880056346485 37.0 37.0 37.0 35.0 37.0 5 36.240983727687244 37.0 37.0 37.0 35.0 37.0 6 36.38797321873354 37.0 37.0 37.0 35.0 37.0 7 36.38632143716914 37.0 37.0 37.0 35.0 37.0 8 36.406741080260375 37.0 37.0 37.0 35.0 37.0 9 38.22981453806129 39.0 39.0 39.0 37.0 39.0 10-11 38.21231471086672 39.0 39.0 39.0 37.0 39.0 12-13 38.21678620035419 39.0 39.0 39.0 37.0 39.0 14-15 39.732189718911116 41.0 40.0 41.0 37.0 41.0 16-17 39.75813436873581 41.0 40.0 41.0 37.0 41.0 18-19 39.80424362467137 41.0 40.0 41.0 37.5 41.0 20-21 39.82792988628211 41.0 40.0 41.0 37.5 41.0 22-23 39.78278357371164 41.0 40.0 41.0 37.0 41.0 24-25 39.75027350928501 41.0 40.0 41.0 37.0 41.0 26-27 39.65381470967496 41.0 40.0 41.0 37.0 41.0 28-29 39.57696396351303 41.0 40.0 41.0 37.0 41.0 30-31 39.46150014181963 41.0 39.5 41.0 36.5 41.0 32-33 39.317336317504356 41.0 39.0 41.0 35.5 41.0 34-35 39.22402615201634 41.0 39.0 41.0 35.0 41.0 36-37 39.208524909009 41.0 39.0 41.0 35.0 41.0 38-39 39.25243655657173 41.0 39.0 41.0 35.0 41.0 40-41 39.17188300714818 41.0 39.0 41.0 35.0 41.0 42-43 39.089067494225915 41.0 39.0 41.0 35.0 41.0 44-45 38.967081161347835 41.0 39.0 41.0 35.0 41.0 46-47 38.7508753488882 41.0 39.0 41.0 35.0 41.0 48-49 38.52223827127831 41.0 39.0 41.0 35.0 41.0 50-51 38.37919946990445 41.0 39.0 41.0 35.0 41.0 52-53 38.30839929733737 41.0 39.0 41.0 35.0 41.0 54-55 38.21718424872541 41.0 39.0 41.0 34.5 41.0 56-57 38.067115245729326 41.0 38.5 41.0 34.0 41.0 58-59 37.91567690866577 40.0 38.0 41.0 34.0 41.0 60-61 37.708669112161445 40.0 38.0 41.0 34.0 41.0 62-63 37.53276152612222 40.0 37.0 41.0 33.5 41.0 64-65 37.2972193818571 40.0 37.0 41.0 33.0 41.0 66-67 37.011631593123056 39.0 36.5 41.0 33.0 41.0 68-69 36.645353202382566 39.0 36.0 41.0 32.0 41.0 70-71 36.240874085620916 38.5 35.0 40.5 32.0 41.0 72-73 35.88995154297373 37.0 35.0 40.0 31.5 41.0 74-75 35.539647524592 37.0 35.0 39.0 31.0 41.0 76-77 35.148777133432006 36.5 35.0 39.0 31.0 41.0 78-79 34.75580566658801 36.0 35.0 39.0 31.0 40.0 80-81 34.45339258771961 36.0 35.0 37.0 31.0 39.0 82-83 34.18334060307903 35.0 35.0 37.0 31.0 39.0 84-85 33.88789813775334 35.0 35.0 37.0 31.0 39.0 86-87 33.67309860397047 35.0 35.0 36.0 31.0 37.0 88-89 33.43210772571369 35.0 34.5 36.0 30.5 37.0 90-91 33.21310484880121 35.0 34.0 36.0 30.0 37.0 92-93 33.075968842584984 35.0 34.0 35.0 30.0 36.0 94-95 32.95983405911615 35.0 34.0 35.0 30.0 36.0 96-97 32.80347374668392 35.0 34.0 35.0 29.5 36.0 98-99 32.674869561693924 35.0 34.0 35.0 29.0 36.0 100-101 32.67720779793444 35.0 34.0 35.0 29.0 36.0 102-103 31.80581555820921 34.5 32.5 35.0 27.5 35.0 104-105 32.48604804706028 35.0 34.0 35.0 29.0 35.0 106-107 32.523644549955065 35.0 34.0 35.0 29.0 35.0 108-109 32.54435855815916 35.0 34.0 35.0 29.0 35.0 110-111 32.54027796647337 35.0 34.0 35.0 29.0 35.0 112-113 32.445923817832096 35.0 34.0 35.0 29.0 35.0 114-115 32.36155424779584 35.0 34.0 35.0 29.0 35.0 116-117 32.28583686690645 35.0 34.0 35.0 29.0 35.0 118-119 32.19448476571159 35.0 34.0 35.0 29.0 35.0 120-121 32.08044152383404 35.0 34.0 35.0 29.0 35.0 122-123 31.8857005293805 35.0 34.0 35.0 28.0 35.0 124-125 29.78065747103423 33.5 29.5 34.5 14.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07815966754822057 1101 2 0.04896706629644143 1101 3 0.17119914256490176 1101 4 -0.009489333384003373 1101 5 0.04781919441172988 1101 6 0.12669095818424836 1101 7 0.09879789185632148 1101 8 0.13190574012354261 1101 9 0.015737602186163713 1101 10-11 0.21937382943072237 1101 12-13 -0.01478835937169265 1101 14-15 0.22329888839085754 1101 16-17 0.20678115347626402 1101 18-19 0.24576055225319493 1101 20-21 0.23410617268757505 1101 22-23 0.23428781217437233 1101 24-25 0.26354813504298846 1101 26-27 0.24773278528871856 1101 28-29 0.3321418593209202 1101 30-31 0.2926791705075331 1101 32-33 0.31870373845580247 1101 34-35 0.27943850982170204 1101 36-37 0.265274597235134 1101 38-39 0.2314866858081217 1101 40-41 0.22738176224365958 1101 42-43 0.28920484201049135 1101 44-45 0.17701258552630605 1101 46-47 0.013834140414765272 1101 48-49 0.1352082390586773 1101 50-51 0.276783763752384 1101 52-53 0.15020212686437162 1101 54-55 0.3686454940990558 1101 56-57 0.3923876552689336 1101 58-59 0.4344405586144262 1101 60-61 0.4370761649999366 1101 62-63 0.2868492691741693 1101 64-65 0.2969821477184027 1101 66-67 0.3814048798577119 1101 68-69 0.07409256131870023 1101 70-71 0.05347800880091569 1101 72-73 -0.03463422933052129 1101 74-75 0.06285599819160126 1101 76-77 0.10776615091039332 1101 78-79 0.12717549341039103 1101 80-81 0.06622537537985096 1101 82-83 0.21110947493228593 1101 84-85 0.24298421390734148 1101 86-87 0.21520982918840303 1101 88-89 0.25241839770572483 1101 90-91 0.11744636387027896 1101 92-93 0.23466165790404858 1101 94-95 0.21095869795141908 1101 96-97 0.14209748530509358 1101 98-99 0.2404783208718939 1101 100-101 0.26227052968767595 1101 102-103 0.2503560885544438 1101 104-105 0.2871587991282212 1101 106-107 0.2708394253572095 1101 108-109 0.25644858935690706 1101 110-111 0.21390601144425858 1101 112-113 0.17381434408331842 1101 114-115 0.3398622966850553 1101 116-117 0.38259631066878086 1101 118-119 0.3151374994287366 1101 120-121 0.1744608147415505 1101 122-123 0.2116490870031491 1101 124-125 0.2751299315559592 1102 1 0.11231781050160805 1102 2 0.02001502366766772 1102 3 0.040645458483318464 1102 4 -0.04137822907824784 1102 5 -0.08830501701719129 1102 6 0.0010401956436680848 1102 7 0.011807017843949552 1102 8 -0.05664696398621771 1102 9 -0.07248129902042422 1102 10-11 0.12636837833743897 1102 12-13 -0.04517361079571458 1102 14-15 -0.09974576157544846 1102 16-17 0.0850181229133895 1102 18-19 -0.05958795022736041 1102 20-21 -0.07591710164328447 1102 22-23 0.08865328041716225 1102 24-25 0.10999879958638559 1102 26-27 0.07841968429037394 1102 28-29 0.1314038180396082 1102 30-31 -0.0064572293209721465 1102 32-33 0.046646716345193795 1102 34-35 0.028683625274631197 1102 36-37 0.14813733993128153 1102 38-39 0.15880952527648873 1102 40-41 0.05519305641895045 1102 42-43 0.0815641241811278 1102 44-45 0.026139798118940405 1102 46-47 0.0606507631868638 1102 48-49 0.011872659956573273 1102 50-51 -0.2091184228097518 1102 52-53 -0.21275800053197003 1102 54-55 -0.07778985838456265 1102 56-57 0.03188358226103105 1102 58-59 0.10394046674206692 1102 60-61 -0.006428459242108886 1102 62-63 -0.0600852564582226 1102 64-65 -0.05710770344837357 1102 66-67 0.008536318578848068 1102 68-69 -0.03902528029335173 1102 70-71 -0.13912320391421673 1102 72-73 -0.2510028212334987 1102 74-75 -0.22148435827314472 1102 76-77 -0.19604502076841612 1102 78-79 -0.29916737423216233 1102 80-81 -0.305505499857496 1102 82-83 -0.3438899125853254 1102 84-85 -0.2620947963211151 1102 86-87 -0.2114193739720136 1102 88-89 -0.15231609117771683 1102 90-91 -0.24827146549212387 1102 92-93 -0.09861297920013357 1102 94-95 -0.15226632807907947 1102 96-97 -0.07791843923707376 1102 98-99 -0.0579629114426794 1102 100-101 -0.16866595588836475 1102 102-103 -0.0066644908539039704 1102 104-105 -0.08433627038853331 1102 106-107 -0.08380472237354297 1102 108-109 -0.0025579642046835716 1102 110-111 -0.15033114173758833 1102 112-113 -0.20655406407494326 1102 114-115 -0.06884107809292317 1102 116-117 0.012813129435237158 1102 118-119 -0.00803117822177768 1102 120-121 -0.19290918587093842 1102 122-123 -0.34985792592022946 1102 124-125 -0.11328649522177159 1103 1 0.08506382951868119 1103 2 0.06519774792015909 1103 3 0.1065006538092419 1103 4 0.14564777619153801 1103 5 -0.02420832782026139 1103 6 0.17093365422999085 1103 7 0.19448544607169538 1103 8 0.15271264192511325 1103 9 0.23906068746141784 1103 10-11 0.21094278548602574 1103 12-13 0.22721727017575688 1103 14-15 0.21657913245593363 1103 16-17 0.2680201850052697 1103 18-19 0.24737447387619937 1103 20-21 0.22827009047493618 1103 22-23 0.1951288217162812 1103 24-25 0.26224991655281826 1103 26-27 0.2817505354760783 1103 28-29 0.3038347952420395 1103 30-31 0.22736107747247303 1103 32-33 0.22998673238367928 1103 34-35 0.18142448906201025 1103 36-37 0.1425811479276149 1103 38-39 0.03762725926663535 1103 40-41 0.0741270694788696 1103 42-43 0.02964523312832057 1103 44-45 -0.09499664362696336 1103 46-47 -0.2263952901984041 1103 48-49 -0.2119044049993235 1103 50-51 -0.29294532165268805 1103 52-53 -0.28483383968880105 1103 54-55 -0.36546313138261155 1103 56-57 -0.26137452290565477 1103 58-59 -0.32502906012240373 1103 60-61 -0.2078891076554541 1103 62-63 -0.20090321956968893 1103 64-65 -0.2018465051438696 1103 66-67 -0.10533411331483222 1103 68-69 0.04456104116385973 1103 70-71 -0.03696946350866881 1103 72-73 0.0014946011516485669 1103 74-75 -0.08647748463356209 1103 76-77 -0.0846032748639658 1103 78-79 0.04237232103648125 1103 80-81 -0.08640823785726326 1103 82-83 -0.05023370912559244 1103 84-85 -0.14424338149697036 1103 86-87 -0.036339181510093965 1103 88-89 -0.20015089470618364 1103 90-91 -0.21043494355375714 1103 92-93 -0.21161275007881386 1103 94-95 -0.15162192436731914 1103 96-97 -0.0629797291901042 1103 98-99 -0.05483540542824272 1103 100-101 0.0221119109921446 1103 102-103 -0.061037844044417255 1103 104-105 -0.11964386576028119 1103 106-107 -0.09031463187062627 1103 108-109 -0.05166439105769882 1103 110-111 -0.016604483472043796 1103 112-113 -0.08673220590103625 1103 114-115 0.05557752655824544 1103 116-117 -0.10714333751401028 1103 118-119 0.02858527268897859 1103 120-121 0.11051617785915369 1103 122-123 0.0879155876552069 1103 124-125 0.2201680814765936 1104 1 0.07795194258810767 1104 2 0.1166251931591944 1104 3 0.14622698975158244 1104 4 0.0638526300684461 1104 5 0.003980196308347672 1104 6 0.009573217268453504 1104 7 -0.05277509787784851 1104 8 -0.05769898035091359 1104 9 -0.09717446091405435 1104 10-11 -0.006594801089796931 1104 12-13 0.07715251427734415 1104 14-15 0.04449737109550256 1104 16-17 0.05949211473104299 1104 18-19 -0.04279952240737117 1104 20-21 -0.05332797247905319 1104 22-23 0.01777367784556816 1104 24-25 0.14594269204693688 1104 26-27 0.09699069539498595 1104 28-29 0.020620650301445664 1104 30-31 0.012771292011585444 1104 32-33 0.020507031950103283 1104 34-35 0.010463286291582108 1104 36-37 -0.07015605574376593 1104 38-39 -0.0997819417018917 1104 40-41 -0.03263569869501026 1104 42-43 -0.06624417505908298 1104 44-45 -0.22125938364610676 1104 46-47 -0.12209324616439687 1104 48-49 -0.07211331315856029 1104 50-51 -0.17318914168760102 1104 52-53 -0.2764634359498501 1104 54-55 -0.15943801735592444 1104 56-57 -0.00227772691488326 1104 58-59 0.031336726239430845 1104 60-61 0.0924318563627935 1104 62-63 0.10280194400934306 1104 64-65 0.033467983284531044 1104 66-67 -0.06561622379403076 1104 68-69 0.060141873417926206 1104 70-71 0.0538633536544566 1104 72-73 0.25346824831646586 1104 74-75 0.017016518407160675 1104 76-77 0.06966216801007619 1104 78-79 0.07757137503727307 1104 80-81 0.09426071872812969 1104 82-83 -0.00661638833049949 1104 84-85 -0.04749806926091793 1104 86-87 -0.025519315526658204 1104 88-89 -0.07581112332266571 1104 90-91 -0.2934351124821859 1104 92-93 -0.1567222720113577 1104 94-95 -0.12808456302940385 1104 96-97 -0.16058939189633747 1104 98-99 -0.05913181851456528 1104 100-101 -0.05522352456166146 1104 102-103 -0.05761422784981107 1104 104-105 0.03056729477803799 1104 106-107 -0.09653119811872557 1104 108-109 -0.08049484322279454 1104 110-111 -0.06789397049270463 1104 112-113 -0.02763822538411631 1104 114-115 -1.970102963113618E-4 1104 116-117 0.22035258004891745 1104 118-119 0.17328091996640893 1104 120-121 0.1384175236556615 1104 122-123 0.3134237806840936 1104 124-125 -0.12458105980388723 1105 1 0.04902885723995354 1105 2 -0.05284442705931269 1105 3 -0.017281676495237264 1105 4 -0.020964324560395653 1105 5 -0.08192654228040652 1105 6 -0.04171508261380552 1105 7 -0.10128417484726526 1105 8 0.004547398444849193 1105 9 0.04525865975624299 1105 10-11 0.07152463704169065 1105 12-13 0.021663891293307813 1105 14-15 -0.034231305556289726 1105 16-17 0.11105149152795235 1105 18-19 -0.022624828395713337 1105 20-21 0.051833441501941024 1105 22-23 0.0023948547027146105 1105 24-25 -0.1319932899907954 1105 26-27 -0.09996174292816562 1105 28-29 0.1262146920856253 1105 30-31 0.009447177118779848 1105 32-33 0.2553746423660215 1105 34-35 0.18987102430532588 1105 36-37 0.24459436182421257 1105 38-39 0.27372234350388425 1105 40-41 0.4032641945171136 1105 42-43 0.25255459824943216 1105 44-45 0.15155899482108026 1105 46-47 0.3119529190556136 1105 48-49 0.31857423647041827 1105 50-51 0.27952984191087893 1105 52-53 0.3789444115899201 1105 54-55 0.20488798686083243 1105 56-57 0.3493227221418209 1105 58-59 0.3344554798332595 1105 60-61 0.22301836823267962 1105 62-63 0.3452514935835609 1105 64-65 0.36698705450383073 1105 66-67 0.17658963037206377 1105 68-69 0.20190140902757747 1105 70-71 0.2648542903758013 1105 72-73 0.24526201080928445 1105 74-75 0.10273295552552497 1105 76-77 0.3484497149009087 1105 78-79 0.23164985043499087 1105 80-81 0.0791666632531971 1105 82-83 0.23093460972541635 1105 84-85 0.15161613900281878 1105 86-87 0.232370665580099 1105 88-89 0.23110544522694454 1105 90-91 0.27091827324109374 1105 92-93 0.16849609550322953 1105 94-95 0.04303616203289096 1105 96-97 0.048291799746827735 1105 98-99 0.04197302527393987 1105 100-101 0.2105441159739243 1105 102-103 0.3084641927848928 1105 104-105 0.2195298094955973 1105 106-107 0.07034128620536251 1105 108-109 0.07356035789436532 1105 110-111 0.10401123473168639 1105 112-113 -0.05471990441809993 1105 114-115 0.1384287118909242 1105 116-117 -0.057302741546884306 1105 118-119 -0.04018119771929918 1105 120-121 -0.2666850200862996 1105 122-123 -0.22318392447184365 1105 124-125 -0.20527162139858746 1106 1 0.08770499953620003 1106 2 0.08245633822166099 1106 3 0.007357994234219234 1106 4 0.05534887685234935 1106 5 -0.11984675788384891 1106 6 -0.07156786652242886 1106 7 -0.06520447640892257 1106 8 -0.07129808912787183 1106 9 -0.05723387578368744 1106 10-11 0.043669117639147714 1106 12-13 0.003679662098356573 1106 14-15 -0.19323504979519157 1106 16-17 -0.10043733011126932 1106 18-19 -0.08745881526433408 1106 20-21 -0.04268899738555376 1106 22-23 -0.04363450761370302 1106 24-25 -0.04708932301898017 1106 26-27 0.002513095331330817 1106 28-29 0.07047078690259667 1106 30-31 0.0929707562167792 1106 32-33 0.1583177001021383 1106 34-35 0.16682916518073654 1106 36-37 0.1764952267155877 1106 38-39 -0.04777403740226305 1106 40-41 -0.08906656259782864 1106 42-43 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12-13 -0.1346174682541701 2216 14-15 -0.14051514011737254 2216 16-17 -0.19023641555553183 2216 18-19 -0.2745024637227047 2216 20-21 -0.20945374557759067 2216 22-23 -0.2368365860702113 2216 24-25 -0.18087143382059168 2216 26-27 -0.11809121764027708 2216 28-29 -0.37639082224470144 2216 30-31 -0.5492725208395584 2216 32-33 -0.3999122117116727 2216 34-35 -0.43433865884792766 2216 36-37 -0.3293624199380858 2216 38-39 -0.22928479803539403 2216 40-41 -0.2595725760851266 2216 42-43 -0.15667427750938145 2216 44-45 -0.07765426585185509 2216 46-47 -0.3972903567823991 2216 48-49 -0.515444600633181 2216 50-51 -0.5508507251822437 2216 52-53 -0.44672687960218127 2216 54-55 -0.5577053324024206 2216 56-57 -0.639343965524958 2216 58-59 -0.4951398329409642 2216 60-61 -0.6115528469090066 2216 62-63 -0.5991833258079424 2216 64-65 -0.37651265974459847 2216 66-67 -0.5101962719503987 2216 68-69 -0.5594724035283889 2216 70-71 -0.735506836610405 2216 72-73 -0.7096331161139204 2216 74-75 -0.6759772023803379 2216 76-77 -0.7842233898011699 2216 78-79 -0.8481828386448029 2216 80-81 -0.8035604417458799 2216 82-83 -0.847258945339135 2216 84-85 -0.763004891412848 2216 86-87 -0.6355164827992112 2216 88-89 -0.5304449933004705 2216 90-91 -0.5701689777387813 2216 92-93 -0.6473955169006445 2216 94-95 -0.4894697667497212 2216 96-97 -0.5610756767742942 2216 98-99 -0.6497696543107949 2216 100-101 -0.6609385521807525 2216 102-103 -0.5982142939467856 2216 104-105 -0.6700677082907482 2216 106-107 -0.6962266799184533 2216 108-109 -0.7562655811482983 2216 110-111 -0.782359765308815 2216 112-113 -0.6657006398121439 2216 114-115 -0.8590917744717501 2216 116-117 -0.9636684765584249 2216 118-119 -0.9926508113021768 2216 120-121 -0.9247168892615818 2216 122-123 -1.1293291582706289 2216 124-125 -1.0720056384356802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42.0 3 4.0 4 13.0 5 12.0 6 29.0 7 81.0 8 145.0 9 284.0 10 589.0 11 948.0 12 1085.0 13 1748.0 14 2950.0 15 3111.0 16 1207.0 17 847.0 18 779.0 19 619.0 20 632.0 21 665.0 22 690.0 23 732.0 24 967.0 25 1226.0 26 1542.0 27 2052.0 28 2529.0 29 3245.0 30 4128.0 31 5502.0 32 6872.0 33 9439.0 34 13452.0 35 22330.0 36 47947.0 37 146314.0 38 133264.0 39 1526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81623569965887 20.14479728618323 15.035651027574417 23.003315986583484 2 13.183981770814713 20.643932622566723 44.95801423916748 21.214071367451083 3 20.383651891206465 25.87171401535466 29.185764646154066 24.55886944728481 4 11.306242208858603 17.528191120422743 39.776711548408166 31.388855122310495 5 11.115539084554937 38.882432532400436 34.46676196089754 15.535266422147078 6 28.20852014196264 37.26590822959049 20.129806672434793 14.39576495601208 7 27.585228830142988 32.267898471446586 22.074284883457633 18.072587814952794 8 24.375576514669394 38.15996777476659 20.09190865385761 17.3725470567064 9 25.252951397578816 18.96092690449461 23.056534786329063 32.72958691159751 10-11 23.934505550033727 26.057390471151027 30.365251092249494 19.64285288656575 12-13 27.594286218230618 24.098134416406268 29.138451711012113 19.169127654351005 14-15 20.74821176173349 29.813465475858486 26.926184670609015 22.51213809179901 16-17 19.104176647669988 30.4598769625334 31.269202258626567 19.166744131170045 18-19 20.312980428891162 27.929886282109035 32.90990044023673 18.84723284876307 20-21 21.20509669830154 27.759152282394556 30.43681388422424 20.598937135079666 22-23 19.507872795831684 30.27796713590405 30.89172581790793 19.322434250356338 24-25 20.988470898373723 27.938466965560472 32.7642671738804 18.308794962185402 26-27 18.81874974675066 29.59668404255066 32.52138616174112 19.063180048957566 28-29 18.354677783418783 30.702042917718398 29.937647033586224 21.005632265276596 30-31 19.810295390027814 27.877329595968988 31.697759726562218 20.61461528744098 32-33 18.784546189103963 29.23879803693031 32.93886024688533 19.037795527080398 34-35 19.558118637482806 29.400281732439986 32.45035717094867 18.591242459128537 36-37 18.77000669770014 28.578204587328713 32.27290387012659 20.378884844844556 38-39 19.86845335564311 30.0099869621282 30.9252598636148 19.196299818613884 40-41 21.99384097610041 27.346518983570373 32.19305584356461 18.466584196764604 42-43 18.836745346766868 30.222954758346503 31.828376796878537 19.11192309800809 44-45 18.89740601172217 29.851363494435667 30.665098308413597 20.586132185428568 46-47 21.450755219319888 28.266201402941743 30.268122522625596 20.014920855112777 48-49 20.48399821712466 29.100672868593985 29.991872185952946 20.42345672832841 50-51 18.43106970136838 30.929192676863376 30.694534819698386 19.94520280206985 52-53 18.833646766631627 29.789272715571798 30.994024507385348 20.38305601041123 54-55 19.19868334179484 28.421249585862846 31.14978774726074 21.23027932508158 56-57 20.37662049782265 28.99031574531578 28.594770073436347 22.038293683425216 58-59 20.96856847981275 29.926563650794787 27.91641937613664 21.18844849325582 60-61 19.63117362297907 27.64016903946399 32.53342295380494 20.195234383752002 62-63 20.026123414063264 27.78961594290985 31.582873909240206 20.60138673378668 64-65 20.6688404398077 29.699294715490755 28.90844172404999 20.72342312065156 66-67 20.63416017752481 30.72015769389365 27.988282600042425 20.657399528539113 68-69 21.29439609864926 27.855282006545156 31.053374234591118 19.79694766021447 70-71 22.723914126426838 27.75290968592315 29.7206272479603 19.802548939689714 72-73 21.06748469182237 29.54424653256965 29.648287319418326 19.73998145618965 74-75 19.83103204170212 30.589659799736385 30.80381935754516 18.775488801016333 76-77 20.56205860130093 28.02177110073484 31.417695752800046 19.99847454516419 78-79 20.119199994279544 28.15644016045878 30.66867359318503 21.055686252076644 80-81 20.80934913132498 29.035841038072014 30.263832180899875 19.89097764970313 82-83 20.24302402353014 28.631237978104956 31.956371991695804 19.169366006669097 84-85 19.727706311807736 29.000684071152932 31.49051238597821 19.78109723106112 86-87 18.440722970251247 31.314012494428518 28.828116992851815 21.417147542468424 88-89 19.675030449508636 29.026306945348196 30.12463442713212 21.174028178011046 90-91 20.384965122889227 28.694333974107632 31.207535791793244 19.713165111209896 92-93 19.52856295003897 30.49729827647439 30.959463421261503 19.014675352225137 94-95 19.36712541652167 30.501661319616918 30.178097276579923 19.95311598728149 96-97 20.653585891449588 29.701082357876473 30.018925174056783 19.62640657661716 98-99 20.80764069475596 28.729832222203683 31.24709505342718 19.215432029613176 100-101 19.56085968914091 28.75577706430984 31.481931702526772 20.20143154402248 102-103 19.432672460230823 29.830221239148987 30.292983367735598 20.444122932884596 104-105 20.59884921032183 28.928964718333024 30.083091400540596 20.389094670804553 106-107 20.03391943478732 28.34143975248109 30.622241662266838 21.002399150464754 108-109 19.111349993385716 29.67907013882847 30.108104822707375 21.101475045078434 110-111 18.673679292730657 30.411723623396714 30.296117789235073 20.618479294637556 112-113 19.869609782841888 28.264522895759335 29.72169912516984 22.144168196228932 114-115 19.535118758886508 29.08898484362728 28.305005762448854 23.070890635037358 116-117 20.442982534546562 29.06094138257745 27.27733185553311 23.21874422734288 118-119 20.130283377070985 27.767091648849828 29.925729417681453 22.17689555639773 120-121 19.373487945274046 28.104374977654363 30.344778271699102 22.17735880537249 122-123 20.47613542782708 28.640466029229316 28.951994365317624 21.931404177625975 124-125 21.33817512689448 29.04766274611689 28.05395890513811 21.56020322185052 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23.0 1 17.5 2 40.5 3 62.0 4 29.5 5 13.0 6 40.5 7 106.5 8 172.5 9 182.5 10 158.5 11 133.0 12 129.0 13 165.5 14 222.0 15 256.5 16 283.0 17 353.5 18 443.5 19 521.0 20 631.0 21 795.0 22 971.5 23 1190.5 24 1462.0 25 1815.0 26 2289.5 27 2844.5 28 3554.5 29 4411.0 30 5686.0 31 7557.0 32 10174.0 33 14066.0 34 19207.0 35 24418.0 36 28065.5 37 28750.0 38 25838.0 39 21138.0 40 17631.0 41 15452.0 42 13446.5 43 12241.0 44 11780.0 45 11447.5 46 11128.5 47 12625.5 48 16626.0 49 15816.5 50 11570.0 51 9652.0 52 8387.5 53 7532.0 54 6763.0 55 5899.5 56 4935.5 57 4097.0 58 3412.5 59 2728.5 60 2134.5 61 1607.0 62 1232.5 63 885.5 64 587.0 65 462.5 66 347.5 67 265.5 68 176.0 69 111.5 70 87.0 71 64.5 72 57.0 73 51.0 74 38.0 75 26.5 76 14.5 77 5.0 78 4.0 79 2.5 80 1.5 81 0.5 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 1.0 97 2.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015254548358110057 2 0.0 3 0.0 4 0.0 5 0.117746045139162 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004886222520957127 22-23 2.3835231809546964E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 5.958807952386741E-4 92-93 0.0 94-95 2.3835231809546964E-4 96-97 0.0 98-99 9.534092723818786E-4 100-101 0.0 102-103 2.3835231809546964E-4 104-105 0.0021451708628592264 106-107 0.005601279475243536 108-109 1.1917615904773482E-4 110-111 0.004290341725718453 112-113 0.008819035769532376 114-115 0.004886222520957127 116-117 0.0010725854314296132 118-119 0.0 120-121 4.767046361909393E-4 122-123 5.958807952386741E-4 124-125 0.0013109377495250831 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 419547.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.72386591268872 #Duplication Level Percentage of deduplicated Percentage of total 1 91.4463565493699 71.0756435464715 2 6.020908524583487 9.35936573674583 3 1.1193333252990065 2.609967398614319 4 0.4501329469683795 1.3994429125221501 5 0.24342860782584932 0.94601062369844 6 0.13774470152182972 0.6423630426759614 7 0.09888987100749923 0.5380272152016968 8 0.06660081305258657 0.4141178130700229 9 0.054877249862087786 0.38387448089442233 >10 0.3044114153437434 4.379763438344344 >50 0.03156977284862721 1.6709151125578536 >100 0.020842179970847835 3.0988399847619124 >500 0.004291037052821613 2.385115769329356 >1k 6.130052932602305E-4 1.0965529251121682 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3175 0.7567686099531161 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1428 0.3403671102403306 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 992 0.2364454995507059 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 934 0.22262106510116866 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 888 0.21165685846877705 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 884 0.21070344919639517 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 865 0.20617475515258124 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 822 0.19592560547447602 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.164939804122065 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 630 0.15016196040014587 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 609 0.14515656172014102 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 587 0.13991281072204068 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 558 0.13300059349727206 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 536 0.12775684249917174 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 510 0.12155968222868953 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 505 0.12036792063821217 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 468 0.1115488848686798 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 455 0.10845030473343867 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.3835231809546964E-4 0.0 22-23 0.0 0.0 0.0 2.3835231809546964E-4 0.0 24-25 0.0 0.0 0.0 2.3835231809546964E-4 0.0 26-27 0.0 0.0 0.0 3.575284771432045E-4 0.0 28-29 0.0 0.0 0.0 8.342331133341437E-4 0.0 30-31 0.0 0.0 0.0 0.0025026993400024313 0.0 32-33 0.0 0.0 0.0 0.005124574839052597 0.0 34-35 0.0 0.0 0.0 0.01012997351905746 0.0 36-37 0.0 0.0 0.0 0.019187361606685306 0.0 38-39 0.0 0.0 0.0 0.03479943844193857 0.0 40-41 0.0 0.0 0.0 0.054940209321005753 0.0 42-43 1.1917615904773482E-4 0.0 0.0 0.07841791265340951 0.0 44-45 2.3835231809546964E-4 0.0 0.0 0.10404078684867249 0.0 46-47 2.3835231809546964E-4 0.0 0.0 0.13371565045155848 0.0 48-49 2.3835231809546964E-4 0.0 0.0 0.16458227564492178 0.0 50-51 2.3835231809546964E-4 0.0 0.0 0.1961639577925715 0.0 52-53 2.3835231809546964E-4 0.0 0.0 0.23322774325641704 0.0 54-55 2.3835231809546964E-4 0.0 0.0 0.2731517565374082 0.0 56-57 2.3835231809546964E-4 0.0 0.0 0.32451668108698195 0.0 58-59 2.3835231809546964E-4 0.0 0.0 0.38517734604227893 0.0 60-61 2.3835231809546964E-4 0.0 0.0 0.45501457524425154 0.0 62-63 2.3835231809546964E-4 0.0 0.0 0.5279503845814653 0.0 64-65 2.3835231809546964E-4 0.0 0.0 0.6006478416005835 0.0 66-67 2.3835231809546964E-4 0.0 0.0 0.682641039025425 0.0 68-69 2.3835231809546964E-4 0.0 0.0 0.784655831170286 0.0 70-71 2.3835231809546964E-4 0.0 0.0 0.8972773014703954 0.0 72-73 2.3835231809546964E-4 0.0 0.0 1.0131165280647938 0.0 74-75 2.3835231809546964E-4 0.0 0.0 1.1171573149134661 0.0 76-77 2.3835231809546964E-4 0.0 0.0 1.233711598462151 0.0 78-79 2.3835231809546964E-4 0.0 0.0 1.3792256886594352 0.0 80-81 2.3835231809546964E-4 0.0 0.0 1.5333204623081562 0.0 82-83 2.3835231809546964E-4 0.0 0.0 1.6936123962273595 0.0 84-85 2.3835231809546964E-4 0.0 0.0 1.8483030506713192 0.0 86-87 2.3835231809546964E-4 0.0 0.0 2.0097867461809997 0.0 88-89 2.3835231809546964E-4 0.0 0.0 2.194867321182132 0.0 90-91 7.15056954286409E-4 0.0 0.0 2.392938097519467 0.0 92-93 7.15056954286409E-4 0.0 0.0 2.593511573196805 0.0 94-95 7.15056954286409E-4 0.0 0.0 2.7891988263531857 0.0 96-97 7.15056954286409E-4 0.0 0.0 3.007529549728636 0.0 98-99 9.534092723818786E-4 0.0 0.0 3.242664111529817 0.0 100-101 0.001310937749525083 0.0 0.0 3.4999654389138763 0.0 102-103 0.001430113908572818 0.0 0.0 3.779076003403671 0.0 104-105 0.001430113908572818 0.0 0.0 4.056041397030607 0.0 106-107 0.001430113908572818 0.0 0.0 4.336105370792783 0.0 108-109 0.001430113908572818 0.0 0.0 4.636667643911171 0.0 110-111 0.001430113908572818 0.0 0.0 4.967977366063874 0.0 112-113 0.001430113908572818 0.0 0.0 5.346719199517574 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGTG 40 1.9810104E-6 74.36606 6 TACGACG 45 3.961977E-6 66.17416 4 ACGACGT 50 7.401819E-6 59.556736 5 TACCTGG 550 0.0 57.391037 2 GTACACG 45 3.1500115E-4 52.951958 1 GACGGAC 35 0.007763511 51.048634 3 ACCTGGG 640 0.0 48.389847 3 GTACCTG 655 0.0 48.202446 1 TACACGG 50 5.303721E-4 47.64539 2 TGTACGA 60 0.0013014489 39.70449 2 GTACGAC 65 0.0019284515 36.6503 3 TACATGG 2260 0.0 35.048874 2 GTACATG 2290 0.0 34.858112 1 ACATGGG 2120 0.0 34.554146 3 CATGGGT 450 0.0 33.087074 4 TAGGACC 860 0.0 31.163408 4 CCTGGGG 355 0.0 30.19778 4 CTGTACG 80 0.0053272354 29.785475 1 TGGATAG 140 3.0653773E-6 29.778368 5 CATGGGG 1055 0.0 28.79046 4 >>END_MODULE