FastQCFastQC Report
Fri 27 May 2016
SRR939940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661449
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA6680.10099040137637218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA100.00130925892495.5483108-109
CCGTTCG304.6772147E-61663.699108-109
CTCGTCG406.381262E-61498.6494110-111
CCGCTCC2350.01486.7096108-109
CATCGCG401.1083444E-51247.7742108-109
CGTGATC250.0056658741198.9196110-111
CCTCGCG2000.01122.9968108-109
CGCGGCG551.6582662E-51089.9269110-111
CCGCTCG704.1061867E-81069.5208108-109
CCGCGCC2650.01035.8881108-109
GTCGTTG300.008157962999.0996110-111
CGGTAGT300.008157962999.0996110-111
CGGTACG300.008157962999.0996110-111
CGCTTCG300.008157962999.0996110-111
CGGCGCG250.008179618998.2194108-109
CGTCGCC552.879981E-5907.47217108-109
CGGCGCC1400.0891.26733108-109
CTCCGCT703.4174318E-5856.37115110-111
CGCGTTC400.008780287749.3247110-111
CGTGTCT1352.7115493E-7666.0664110-111
Produced by FastQC (version 0.11.5)