##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939940_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 661449 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.05795306970001 33.0 31.0 34.0 28.0 34.0 2 31.129130137017366 33.0 31.0 34.0 28.0 34.0 3 31.20308746403729 34.0 31.0 34.0 27.0 34.0 4 34.46096373265361 37.0 35.0 37.0 31.0 37.0 5 34.43427837973903 37.0 35.0 37.0 31.0 37.0 6 34.54606477596912 37.0 35.0 37.0 31.0 37.0 7 34.422386306427256 37.0 35.0 37.0 31.0 37.0 8 34.43835579160298 37.0 35.0 37.0 31.0 37.0 9 36.01776100651751 39.0 37.0 39.0 31.0 39.0 10-11 36.01240987589368 39.0 37.0 39.0 31.0 39.0 12-13 35.984646586509314 39.0 37.0 39.0 31.0 39.0 14-15 37.39595796501318 41.0 38.0 41.0 31.0 41.0 16-17 37.37598817142364 41.0 38.0 41.0 31.0 41.0 18-19 37.3619440047532 41.0 38.0 41.0 31.0 41.0 20-21 37.38967781340662 41.0 38.0 41.0 31.0 41.0 22-23 37.36167036309678 40.0 38.0 41.0 31.0 41.0 24-25 37.364770375342616 40.0 38.0 41.0 31.0 41.0 26-27 37.31164761001982 40.0 38.0 41.0 31.0 41.0 28-29 37.25014324611573 40.0 38.0 41.0 31.0 41.0 30-31 37.18122788000284 40.0 38.0 41.0 30.5 41.0 32-33 37.05756301695217 40.0 38.0 41.0 30.0 41.0 34-35 36.96432226823232 40.0 38.0 41.0 30.0 41.0 36-37 37.145199403128586 40.0 38.0 41.0 30.0 41.0 38-39 37.11547980267564 40.0 38.0 41.0 30.0 41.0 40-41 37.04938173615804 40.0 38.0 41.0 30.0 41.0 42-43 36.945738068997 40.0 38.0 41.0 30.0 41.0 44-45 36.8254914589031 40.0 37.5 41.0 30.0 41.0 46-47 36.7166213872876 40.0 37.0 41.0 30.0 41.0 48-49 36.59561054593779 40.0 37.0 41.0 30.0 41.0 50-51 36.45720834108147 40.0 37.0 41.0 30.0 41.0 52-53 36.31693448776852 40.0 36.5 41.0 29.5 41.0 54-55 36.14350312722523 40.0 36.0 41.0 29.0 41.0 56-57 35.94651515082795 40.0 35.5 41.0 28.5 41.0 58-59 35.75233842669654 39.5 35.0 41.0 28.0 41.0 60-61 35.500136064912034 39.0 35.0 41.0 28.0 41.0 62-63 35.23044482643408 39.0 35.0 41.0 28.0 41.0 64-65 34.92324124762453 38.5 35.0 41.0 27.0 41.0 66-67 34.61254382424042 37.5 34.5 40.0 26.0 41.0 68-69 34.24522147588098 37.0 34.0 40.0 26.0 41.0 70-71 33.86172478906159 36.5 34.0 39.5 26.0 41.0 72-73 33.47579631989768 36.0 34.0 39.0 25.5 41.0 74-75 33.06851397462238 35.5 34.0 39.0 24.5 40.5 76-77 32.64755710568767 35.0 33.0 37.5 22.5 39.5 78-79 32.233675612178715 35.0 33.0 37.0 21.0 39.0 80-81 31.83634868296724 35.0 33.0 36.5 19.5 39.0 82-83 31.657752903096082 35.0 33.0 36.0 15.5 38.0 84-85 31.270580195903236 35.0 33.0 36.0 2.0 37.0 86-87 30.59215374125594 35.0 33.0 35.5 2.0 37.0 88-89 15.545323978114716 18.0 16.0 18.5 2.0 19.0 90-91 2.683538715758887 2.0 2.0 2.0 2.0 2.0 92-93 2.2566010380240957 2.0 2.0 2.0 2.0 2.0 94-95 2.088880624205343 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24958870426031154 1101 2 0.21858079102603867 1101 3 0.30831719589990314 1101 4 0.37071282609122846 1101 5 0.33865084698356895 1101 6 0.31625259947752227 1101 7 0.2594715898094151 1101 8 0.4437167492373604 1101 9 0.3535679347280478 1101 10-11 0.3905795754808139 1101 12-13 0.3346431727056469 1101 14-15 0.46005009468807856 1101 16-17 0.5579038786825663 1101 18-19 0.46588646639335707 1101 20-21 0.41340404462661695 1101 22-23 0.42342956334292126 1101 24-25 0.33902134221942504 1101 26-27 0.2912121291530525 1101 28-29 0.3902575613539341 1101 30-31 0.25835489261091027 1101 32-33 0.37522444700332613 1101 34-35 0.25830579816912547 1101 36-37 0.3338036738452743 1101 38-39 0.24657595960959355 1101 40-41 0.27040846145647635 1101 42-43 0.22003175900626104 1101 44-45 0.11172271981122606 1101 46-47 0.002847376757337372 1101 48-49 0.10721516341626369 1101 50-51 0.25799454588729986 1101 52-53 0.20684422604196584 1101 54-55 0.18739431011491092 1101 56-57 0.2524948242291245 1101 58-59 0.11514777095128181 1101 60-61 0.21505940646006394 1101 62-63 0.0903292035164256 1101 64-65 0.09782384536576672 1101 66-67 0.060913783958220336 1101 68-69 0.11016710937464325 1101 70-71 0.14051774245784543 1101 72-73 0.0060772140440832345 1101 74-75 0.0378858185775357 1101 76-77 0.13177995388905828 1101 78-79 0.01883950283062319 1101 80-81 0.13396404698580255 1101 82-83 0.24971356844559978 1101 84-85 0.43435905320163215 1101 86-87 0.4500613796622126 1101 88-89 0.6277465500591006 1101 90-91 -0.01208871448425164 1101 92-93 -0.15078505692256572 1101 94-95 -0.01179182508229637 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -9.118276274016068E-4 1102 2 -0.09565568299972327 1102 3 -0.0743887886498058 1102 4 -0.09771301854181758 1102 5 -0.13072264038815717 1102 6 -0.07613420599093246 1102 7 -0.05320406088203811 1102 8 0.054110160333138424 1102 9 -0.07087149278424931 1102 10-11 0.09259819387344947 1102 12-13 0.07692267156987498 1102 14-15 -0.008411751078419627 1102 16-17 -0.012084647407803573 1102 18-19 -0.020246129265139245 1102 20-21 0.043130064387753464 1102 22-23 0.21009265312278558 1102 24-25 0.1855519942812407 1102 26-27 0.2869430915214153 1102 28-29 0.2513802857339016 1102 30-31 0.19152982095704374 1102 32-33 0.28152743254248236 1102 34-35 0.12034132548120624 1102 36-37 0.39638061175145367 1102 38-39 0.18209560693784255 1102 40-41 0.09702584141012238 1102 42-43 0.2514758457620232 1102 44-45 0.26801525340840726 1102 46-47 0.3514986117123513 1102 48-49 0.3383772800041953 1102 50-51 0.2760160164987582 1102 52-53 0.24190933002717685 1102 54-55 0.24067250364771553 1102 56-57 0.1339523823767692 1102 58-59 0.24205525036096276 1102 60-61 0.30985155217406657 1102 62-63 0.4153125965419662 1102 64-65 0.15327255884784563 1102 66-67 0.1990245838337188 1102 68-69 0.025060429886927693 1102 70-71 0.12181573429139547 1102 72-73 0.23904403261230556 1102 74-75 0.21425072606623274 1102 76-77 0.1688378948100464 1102 78-79 0.08136882633449005 1102 80-81 0.11888886268322807 1102 82-83 0.08059069541579333 1102 84-85 0.06100755671580771 1102 86-87 -0.07804040831666015 1102 88-89 0.9963412516213168 1102 90-91 0.31513720075746665 1102 92-93 0.11153894398791442 1102 94-95 0.17672985224535154 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -4.651886418791662E-4 1103 2 0.06089015921775953 1103 3 -0.0021724268497820276 1103 4 -0.1116171819353724 1103 5 -0.11443939496115973 1103 6 -0.03458331383517077 1103 7 -0.09975326972504206 1103 8 -0.1835691124757517 1103 9 -0.02474647875664715 1103 10-11 -0.061852485062701135 1103 12-13 -0.037448330562327214 1103 14-15 -0.005810471760725022 1103 16-17 0.03912621557053342 1103 18-19 0.016004572115178917 1103 20-21 0.18887016089768593 1103 22-23 0.16556005296567378 1103 24-25 0.19236155549771894 1103 26-27 0.32099426422929866 1103 28-29 0.22875544003757398 1103 30-31 0.10215376888656635 1103 32-33 0.1426765740529703 1103 34-35 0.20803518700068224 1103 36-37 0.182872587960631 1103 38-39 0.2758684719361568 1103 40-41 0.2800793707558782 1103 42-43 0.18633837521999652 1103 44-45 0.19421096657191583 1103 46-47 0.23669555930521113 1103 48-49 0.2739815892848583 1103 50-51 0.13530678072275037 1103 52-53 0.3161344450535495 1103 54-55 0.27956263943494974 1103 56-57 0.15206482766910057 1103 58-59 -0.012365128945518222 1103 60-61 0.05291630360488142 1103 62-63 0.18362928378245158 1103 64-65 0.1388948234646037 1103 66-67 0.036994508645747715 1103 68-69 -0.005546684915003652 1103 70-71 -0.02976433528553457 1103 72-73 0.1201157417358587 1103 74-75 -0.10086317809715695 1103 76-77 0.030850014661908176 1103 78-79 0.07278056463545823 1103 80-81 -0.06121999154188629 1103 82-83 -0.06767549244191429 1103 84-85 0.06864755222697383 1103 86-87 -0.10791247654693592 1103 88-89 0.675627527011283 1103 90-91 -0.49861434227922974 1103 92-93 -0.2727894142210405 1103 94-95 -0.09512515841562941 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.11375803994375033 1104 2 0.3362941466259457 1104 3 0.34141071779041354 1104 4 0.321740083210706 1104 5 0.18258937844139211 1104 6 0.2880753264696878 1104 7 0.3966624810895638 1104 8 0.2133503109516539 1104 9 0.5209684381263315 1104 10-11 0.4220471117783049 1104 12-13 0.5062872413159525 1104 14-15 0.6052703257209089 1104 16-17 0.4738155622320477 1104 18-19 0.36634642885566393 1104 20-21 0.4247579391050067 1104 22-23 0.2971782116888164 1104 24-25 0.40888499946966306 1104 26-27 0.2744150407245769 1104 28-29 0.3290382036096702 1104 30-31 0.3331782345628298 1104 32-33 0.30892995672266466 1104 34-35 0.43685387590552693 1104 36-37 0.32564002596802766 1104 38-39 0.2925156249708962 1104 40-41 0.45641835567882794 1104 42-43 0.36181084443079925 1104 44-45 0.35797580889808955 1104 46-47 0.2500257630938023 1104 48-49 0.2530206349577995 1104 50-51 0.3118583905973864 1104 52-53 0.32154450746035224 1104 54-55 0.3249401177582456 1104 56-57 0.2084629057604488 1104 58-59 0.15797948915798088 1104 60-61 -0.02906304139981586 1104 62-63 -0.013332664635285596 1104 64-65 -0.07791690680545571 1104 66-67 -0.009564833045310195 1104 68-69 -0.04455756468525607 1104 70-71 -0.012891855029280919 1104 72-73 0.05583065861883085 1104 74-75 0.13272107108824116 1104 76-77 0.07179108717529914 1104 78-79 0.044040850712669055 1104 80-81 0.10471859574479936 1104 82-83 0.2295244854671843 1104 84-85 0.20510251439630167 1104 86-87 0.17500462733553945 1104 88-89 1.0961864268842625 1104 90-91 -0.487674926583642 1104 92-93 -0.2727894142210405 1104 94-95 -0.09512515841562941 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.11245839061755447 1105 2 -0.05654677317376411 1105 3 -0.020058666161482108 1105 4 -0.08619894411074114 1105 5 0.01297576250870236 1105 6 -0.027102300504665777 1105 7 -0.07807394527871026 1105 8 -0.19876163464898866 1105 9 -0.02988642246050688 1105 10-11 -0.12074621282306452 1105 12-13 0.05030141716724046 1105 14-15 -0.15271259083237254 1105 16-17 0.06842888698165694 1105 18-19 0.013618890453912513 1105 20-21 -0.0817846800391635 1105 22-23 -0.23497263597160156 1105 24-25 -0.053948391923810846 1105 26-27 -0.1425072493932973 1105 28-29 -0.2980362111388075 1105 30-31 -0.1368913211341436 1105 32-33 -0.20057320023137493 1105 34-35 -0.2071573351725675 1105 36-37 -0.21357809980823106 1105 38-39 -0.1313114101606132 1105 40-41 0.03036297583314962 1105 42-43 -0.07527084460048883 1105 44-45 -0.11363172289645718 1105 46-47 -0.09096974421594695 1105 48-49 -0.16984867254529945 1105 50-51 -0.22126871452394425 1105 52-53 -0.23503024770761982 1105 54-55 -0.3497564959413353 1105 56-57 -0.2949105826022844 1105 58-59 -0.18798730749986703 1105 60-61 -0.1617419363441357 1105 62-63 -0.22299507226406945 1105 64-65 -0.1887152461089272 1105 66-67 -0.1669116998624034 1105 68-69 -0.15767319189891538 1105 70-71 -0.262943473848388 1105 72-73 -0.119645796317144 1105 74-75 -0.16701273463229427 1105 76-77 -0.17056588634186198 1105 78-79 -0.10294995743156221 1105 80-81 -0.12555957410070562 1105 82-83 -0.013355029326504564 1105 84-85 -0.04699162138819446 1105 86-87 0.10766774545104596 1105 88-89 0.4993738757286863 1105 90-91 -0.6901932896476506 1105 92-93 -0.2727894142210405 1105 94-95 -0.09512515841562941 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.03195397035712233 1106 2 0.03925669308230084 1106 3 0.07633879143497424 1106 4 -0.11977402823438865 1106 5 -0.05843184620879782 1106 6 -0.006808098905416671 1106 7 -0.24664989375523305 1106 8 -0.0025374496533530078 1106 9 -0.2307288650011401 1106 10-11 -0.2961018035781109 1106 12-13 -0.13631287239499557 1106 14-15 -0.09248377853364076 1106 16-17 -0.13850221806335128 1106 18-19 -0.11072430186259652 1106 20-21 -0.1139994134365594 1106 22-23 -0.08864523116098155 1106 24-25 -0.0683524306628982 1106 26-27 -0.3363961740177075 1106 28-29 -0.03643327877140479 1106 30-31 0.07824309578948885 1106 32-33 0.11868307436750314 1106 34-35 0.005501112771284511 1106 36-37 -0.013310049908945132 1106 38-39 -0.08562030977908819 1106 40-41 0.005098242636769612 1106 42-43 -0.4754596956544006 1106 44-45 -0.24580685687115533 1106 46-47 -0.3273818154064685 1106 48-49 -0.11004650884892442 1106 50-51 -0.1036709923953012 1106 52-53 -0.1277856639839925 1106 54-55 -0.04274287534802568 1106 56-57 -0.17816163490166304 1106 58-59 -0.2204412455318021 1106 60-61 -0.10871087190360385 1106 62-63 0.0649188623104493 1106 64-65 -0.03410161184677918 1106 66-67 0.03283221047002627 1106 68-69 0.14764790514580994 1106 70-71 0.06827403753895567 1106 72-73 -0.10821724211819372 1106 74-75 -0.076058187533107 1106 76-77 0.04061411615068522 1106 78-79 -0.07114478659121204 1106 80-81 0.02942794303559282 1106 82-83 0.12970341298899513 1106 84-85 0.16700255327541313 1106 86-87 0.09095206528573385 1106 88-89 0.6783838700601379 1106 90-91 -0.26838054064366634 1106 92-93 -0.24340694410151853 1106 94-95 -0.09512515841562941 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.029064635723408117 1107 2 0.025587879733119934 1107 3 0.07204279405679515 1107 4 0.008428720730108807 1107 5 0.03390445773301565 1107 6 -0.09070245211695038 1107 7 -0.21175577350679475 1107 8 -0.12457062517722761 1107 9 -0.019489580316289334 1107 10-11 0.05516853627160856 1107 12-13 -0.08151175970008495 1107 14-15 -0.04686101685488353 1107 16-17 -0.004389511308602323 1107 18-19 0.03571768045590318 1107 20-21 0.012048920482470749 1107 22-23 0.09436554042068224 1107 24-25 0.07589762560360214 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-0.11449519174711753 2216 14-15 -0.14575742136285186 2216 16-17 -0.31664464393898584 2216 18-19 -0.19604438316426354 2216 20-21 -0.3332369636776775 2216 22-23 -0.34999813959796455 2216 24-25 -0.43207745981243306 2216 26-27 -0.5870782379811317 2216 28-29 -0.28389568379322583 2216 30-31 -0.3499231076549165 2216 32-33 -0.5149878123772922 2216 34-35 -0.3037178029011329 2216 36-37 -0.4858914308396862 2216 38-39 -0.43411756743282837 2216 40-41 -0.5428677184525625 2216 42-43 -0.4269028179485943 2216 44-45 -0.5174633784418177 2216 46-47 -0.47259803276514845 2216 48-49 -0.40804549433932635 2216 50-51 -0.23857567076404962 2216 52-53 -0.25483581880238404 2216 54-55 -0.34796539816568384 2216 56-57 -0.35566470837259345 2216 58-59 -0.321750861180611 2216 60-61 -0.4936098260428423 2216 62-63 -0.45402393414299524 2216 64-65 -0.4812070891418472 2216 66-67 -0.3762564569645619 2216 68-69 -0.4629895626664151 2216 70-71 -0.2778089162339228 2216 72-73 -0.44965344249793304 2216 74-75 -0.5209492686551158 2216 76-77 -0.523659734512087 2216 78-79 -0.430873863211346 2216 80-81 -0.4939506909694984 2216 82-83 -0.47721991051627555 2216 84-85 -0.5090047349897411 2216 86-87 -0.7425367028953005 2216 88-89 -1.520816234913422 2216 90-91 -0.6901932896476506 2216 92-93 -0.2727894142210405 2216 94-95 -0.09512515841562941 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 17953.0 3 2465.0 4 1499.0 5 1276.0 6 1193.0 7 1318.0 8 1533.0 9 1670.0 10 1932.0 11 2273.0 12 2550.0 13 2897.0 14 3147.0 15 3503.0 16 3845.0 17 4594.0 18 5783.0 19 6834.0 20 7696.0 21 10176.0 22 12044.0 23 15655.0 24 23152.0 25 38369.0 26 77857.0 27 202734.0 28 200652.0 29 6616.0 30 233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29388962716702 18.7346265547306 13.79274894965447 24.178734868447908 2 14.445018943345337 19.282148504942203 42.01854724346356 24.254285308248896 3 19.451023451513475 24.221937888123605 27.97938275301954 28.347655907343384 4 12.232254941996763 16.21353055946277 37.64931863212184 33.90489586641862 5 13.646297951633565 38.00919896969119 34.087007551867096 14.257495526808144 6 32.49955778903589 36.05780642196148 17.97916392647052 13.463471862532108 7 29.609539057432997 31.527600767406106 20.497725448220496 18.3651347269404 8 25.927017804849655 36.78590488457915 19.584276338765346 17.70280097180584 9 26.07033951219217 17.40209751621062 20.154085953716763 36.37347701788044 10-11 24.96362139643947 26.73685626879876 27.55619691086127 20.743325423900497 12-13 25.91575167721818 23.74827553623736 27.714031938013793 22.62194084853066 14-15 21.97775034639885 26.300989419433257 25.12727748267628 26.593982751491613 16-17 22.350375502212948 29.00887825564387 26.542469356978444 22.098276885164733 18-19 22.60528983573493 27.88862421482899 27.643840607922126 21.862245341513955 20-21 23.176543157614645 27.307555077135206 27.14102347014677 22.374878295103382 22-23 22.609043375455727 27.64362294399161 27.11765368424156 22.629679996311108 24-25 22.639009205547215 27.355094648264643 27.51686071034955 22.48903543583859 26-27 21.999277344133862 27.912129279808422 27.331132105423094 22.75746127063462 28-29 22.134350302558424 28.140087762730698 26.868170702668788 22.85739123204209 30-31 22.470666672713996 27.51134252225039 27.129378077523743 22.888612727511873 32-33 22.00255049010381 27.766023147927672 27.42797360008406 22.80345276188446 34-35 22.51942326619286 27.92104909070843 27.24253872936538 22.316988913733336 36-37 22.318139658643112 27.456332579180387 27.265992741687374 22.959535020489124 38-39 22.50959635587929 27.841299933932927 26.996488013437165 22.65261569675062 40-41 23.05128588901034 27.30407030625188 27.25365069718149 22.39099310755629 42-43 22.192489519222192 27.72443529281925 27.27647936575609 22.806595822202468 44-45 22.16478090492374 27.867647948138025 27.28317267570542 22.68439847123281 46-47 22.753757281362585 27.643476670159 26.95332519967526 22.649440848803156 48-49 22.53047667354299 27.68839902123899 27.000590370981264 22.780533934236754 50-51 22.295310972811944 28.145275104562185 27.065070069703083 22.49434385292279 52-53 22.465904400792805 27.75769560464979 27.21721553740349 22.559184457153915 54-55 22.255222711728084 27.63478965526625 27.38472842503757 22.725259207968094 56-57 22.469835164918234 27.752101824932833 26.92754845800659 22.850514552142343 58-59 22.54905518036916 27.663432857257327 26.801008089814935 22.98650387255858 60-61 22.27238985923329 27.44338565785117 27.590864904172506 22.693359578743035 62-63 22.324170117424018 27.556319534839417 27.608931300825912 22.510579046910646 64-65 22.213596372204336 27.772456963769663 27.197884642568543 22.816062021457455 66-67 22.382451254745263 27.973358490223738 26.85989396007856 22.784296294952444 68-69 22.72835162907988 27.326679235070987 27.508553217415756 22.436415918433376 70-71 22.76093848505327 27.319642179518 27.166191195390727 22.753228140038008 72-73 22.553439494201367 27.647860983991208 27.13096550149747 22.667734020309958 74-75 22.19664705820101 28.06951102806112 27.31858389686884 22.415258016869025 76-77 22.54421731683017 27.363485317840073 27.455177950227455 22.637119415102298 78-79 22.39867865552444 27.46264820715174 27.316680462168197 22.821992675155624 80-81 22.656848827347233 27.674393641837842 27.198090858100926 22.470666672713996 82-83 22.36435201388474 27.723372522755085 27.58655097056371 22.325724492796457 84-85 22.36037850234863 27.733657470190447 27.513685862402088 22.392278165058833 86-87 22.151753290537947 28.124234626769994 27.054828692462827 22.66918339022923 88-89 31.41200315912009 17.655607251004067 33.009810490225064 17.922579099650775 90-91 30.426707964402226 12.795396846708803 15.020821397201335 41.75707379168763 92-93 23.06307176992118 15.99709552960805 33.069379201245205 27.87045349922556 94-95 15.45930453200931 6.391586758131627 25.48893885027613 52.66016985958293 96-97 25.92168519837248 7.09262504099826 13.322587603308229 53.66310215732103 98-99 57.38793093974262 14.321980811710791 18.27613113096612 10.013957117580473 100-101 30.987712665406423 7.166824196597353 11.239760554505356 50.60570258349087 102-103 23.761109713237502 2.0510864183502027 35.40822869572981 38.779575172682485 104-105 43.85299799559212 5.497761291044257 35.268811352869506 15.38042936049412 106-107 5.32807369008034 1.5159338276296497 5.675871042359405 87.4801214399306 108-109 16.981950756560806 9.000420071464008 18.576061458356744 55.44156771361845 110-111 39.70606992355788 2.475546072697418 22.00314783558441 35.8152361681603 112-113 28.559016216696158 3.0300868230623363 35.8424183615873 32.56847859865421 114-115 28.95428721665276 4.17119145871675 20.643339696619837 46.23118162801065 116-117 19.607732116366765 4.061965642587791 18.30658951159025 58.0237127294552 118-119 19.860776336885273 4.248966592864855 13.248334005911417 62.64192306433846 120-121 25.627517456960234 4.3199172641171995 26.853392637750577 43.19917264117199 122-123 34.47264455884114 1.6146105716824084 30.98477067804193 32.92797419143453 124-125 38.10987941547679 1.7379073339111657 17.3235710049724 42.82864224563965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 12.5 2 36.0 3 58.0 4 67.0 5 79.5 6 80.0 7 78.5 8 75.0 9 59.0 10 59.0 11 60.0 12 63.0 13 76.0 14 79.0 15 97.0 16 133.5 17 173.0 18 228.0 19 324.5 20 479.5 21 701.5 22 1048.0 23 1515.0 24 2125.0 25 2873.0 26 3778.0 27 4904.5 28 6159.0 29 7536.5 30 9072.0 31 10820.0 32 12790.0 33 14865.0 34 16850.0 35 18593.5 36 20042.0 37 21121.5 38 21966.5 39 22596.5 40 23287.0 41 23812.5 42 24128.0 43 24833.5 44 25360.0 45 25339.0 46 25241.0 47 25178.0 48 25005.0 49 24672.0 50 24264.5 51 23580.0 52 22472.5 53 20956.5 54 19186.5 55 17419.5 56 15478.5 57 13585.0 58 11811.5 59 9840.0 60 8031.0 61 6491.0 62 5143.5 63 3996.0 64 2974.5 65 2154.5 66 1533.5 67 1134.5 68 835.0 69 611.0 70 424.5 71 273.5 72 188.0 73 136.5 74 102.5 75 69.5 76 50.0 77 34.0 78 20.5 79 14.5 80 10.5 81 13.5 82 12.5 83 8.0 84 8.0 85 6.5 86 5.5 87 6.5 88 6.5 89 5.5 90 3.0 91 2.0 92 2.5 93 1.5 94 1.0 95 2.0 96 3.0 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 4.5354970678011455E-4 3 0.03855172507630974 4 0.042180122730550655 5 0.043540771850891 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.7795808898342877E-4 12-13 0.0017386072093237724 14-15 7.559161779668576E-5 16-17 2.2677485339005728E-4 18-19 0.007332386926278518 20-21 0.0019653820627138297 22-23 5.291413245768003E-4 24-25 0.0 26-27 0.0 28-29 9.826910313569148E-4 30-31 0.0 32-33 0.0011338742669502865 34-35 0.0 36-37 7.559161779668576E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.5118323559337152E-4 46-47 0.0 48-49 7.559161779668576E-5 50-51 7.559161779668576E-5 52-53 0.0 54-55 0.0016630155915270865 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.559161779668576E-5 66-67 0.0 68-69 3.0236647118674304E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.2677485339005728E-4 80-81 0.0 82-83 5.291413245768003E-4 84-85 0.0 86-87 0.0010582826491536007 88-89 19.218866458336166 90-91 26.24684593974743 92-93 40.55671714674903 94-95 44.82590494505245 96-97 48.83520876137087 98-99 93.12169192182618 100-101 52.01444102266388 102-103 53.434429562974614 104-105 74.99648498977245 106-107 81.69964728951136 108-109 12.544580156595595 110-111 38.95682055608218 112-113 52.323761922687915 114-115 86.77433936705626 116-117 66.62365503614035 118-119 72.27692535630109 120-121 80.55753353622124 122-123 76.00616222868278 124-125 50.288684388365546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 661449.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.34463209320684 #Duplication Level Percentage of deduplicated Percentage of total 1 87.2623920404645 67.49277607942908 2 8.68312745986046 13.43186597602658 3 2.023076423564844 4.694223050311907 4 0.7357175310294366 2.2761520704797706 5 0.3667794414284437 1.4184210478317443 6 0.22852730599192658 1.0605216243118358 7 0.14721494851989012 0.79704002223239 8 0.09673207311287135 0.5985365285222577 9 0.07181668992105286 0.4999171914086175 >10 0.3544672952216935 4.699920558899924 >50 0.017815323754660967 0.9654485707518957 >100 0.011746159172078812 1.8067083586521548 >500 5.873079581833924E-4 0.2584689211417988 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 668 0.10099040137637218 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.5118323559337152E-4 14-15 0.0 0.0 0.0 0.0 1.5118323559337152E-4 16-17 0.0 0.0 0.0 0.0 1.5118323559337152E-4 18-19 0.0 0.0 0.0 0.0 1.5118323559337152E-4 20-21 0.0 0.0 0.0 0.0 1.5118323559337152E-4 22-23 0.0 0.0 0.0 0.0 1.5118323559337152E-4 24-25 1.5118323559337152E-4 0.0 0.0 3.0236647118674304E-4 1.5118323559337152E-4 26-27 1.5118323559337152E-4 0.0 0.0 3.0236647118674304E-4 1.5118323559337152E-4 28-29 1.5118323559337152E-4 0.0 0.0 7.559161779668576E-4 1.5118323559337152E-4 30-31 1.5118323559337152E-4 0.0 0.0 0.0027968898584773733 1.5118323559337152E-4 32-33 1.5118323559337152E-4 0.0 0.0 0.007559161779668576 3.0236647118674304E-4 34-35 1.5118323559337152E-4 0.0 0.0 0.01247261693645315 4.5354970678011455E-4 36-37 3.0236647118674304E-4 0.0 0.0 0.020560920040698528 4.5354970678011455E-4 38-39 3.0236647118674304E-4 0.0 0.0 0.03635956816020586 4.5354970678011455E-4 40-41 3.0236647118674304E-4 0.0 0.0 0.05586220555175078 4.5354970678011455E-4 42-43 3.0236647118674304E-4 0.0 0.0 0.07506247647210895 4.5354970678011455E-4 44-45 3.0236647118674304E-4 0.0 0.0 0.09789114504670807 4.5354970678011455E-4 46-47 3.0236647118674304E-4 0.0 0.0 0.12283637891961435 4.5354970678011455E-4 48-49 3.0236647118674304E-4 0.0 0.0 0.15254388471371186 4.5354970678011455E-4 50-51 3.0236647118674304E-4 0.0 0.0 0.18444354742391328 4.5354970678011455E-4 52-53 3.0236647118674304E-4 0.0 0.0 0.21399986998241738 4.5354970678011455E-4 54-55 3.0236647118674304E-4 0.0 0.0 0.25360987770788074 4.5354970678011455E-4 56-57 3.0236647118674304E-4 0.0 0.0 0.29488290102487114 4.5354970678011455E-4 58-59 3.0236647118674304E-4 0.0 0.0 0.3376677566977953 4.5354970678011455E-4 60-61 3.0236647118674304E-4 0.0 0.0 0.38680230826564105 4.5354970678011455E-4 62-63 4.5354970678011455E-4 0.0 0.0 0.4449322623512924 4.5354970678011455E-4 64-65 4.5354970678011455E-4 0.0 0.0 0.5013992008454167 4.5354970678011455E-4 66-67 4.5354970678011455E-4 0.0 0.0 0.570111981422604 4.5354970678011455E-4 68-69 4.5354970678011455E-4 0.0 0.0 0.6574202999777761 4.5354970678011455E-4 70-71 4.5354970678011455E-4 0.0 0.0 0.7511539060456665 4.5354970678011455E-4 72-73 4.5354970678011455E-4 0.0 0.0 0.8469284857940673 5.291413245768003E-4 74-75 4.5354970678011455E-4 0.0 0.0 0.9361265947941565 6.047329423734861E-4 76-77 4.5354970678011455E-4 0.0 0.0 1.0397627027934127 6.047329423734861E-4 78-79 4.5354970678011455E-4 0.0 0.0 1.0700749415298836 6.047329423734861E-4 80-81 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 82-83 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 84-85 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 86-87 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 88-89 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 90-91 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 92-93 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 94-95 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 96-97 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 98-99 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 100-101 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 102-103 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 104-105 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 106-107 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 108-109 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 110-111 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 112-113 4.5354970678011455E-4 0.0 0.0 1.0705284912366637 6.047329423734861E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCA 10 0.0013092589 2495.5483 108-109 CCGTTCG 30 4.6772147E-6 1663.699 108-109 CTCGTCG 40 6.381262E-6 1498.6494 110-111 CCGCTCC 235 0.0 1486.7096 108-109 CATCGCG 40 1.1083444E-5 1247.7742 108-109 CGTGATC 25 0.005665874 1198.9196 110-111 CCTCGCG 200 0.0 1122.9968 108-109 CGCGGCG 55 1.6582662E-5 1089.9269 110-111 CCGCTCG 70 4.1061867E-8 1069.5208 108-109 CCGCGCC 265 0.0 1035.8881 108-109 GTCGTTG 30 0.008157962 999.0996 110-111 CGGTAGT 30 0.008157962 999.0996 110-111 CGGTACG 30 0.008157962 999.0996 110-111 CGCTTCG 30 0.008157962 999.0996 110-111 CGGCGCG 25 0.008179618 998.2194 108-109 CGTCGCC 55 2.879981E-5 907.47217 108-109 CGGCGCC 140 0.0 891.26733 108-109 CTCCGCT 70 3.4174318E-5 856.37115 110-111 CGCGTTC 40 0.008780287 749.3247 110-111 CGTGTCT 135 2.7115493E-7 666.0664 110-111 >>END_MODULE