FastQCFastQC Report
Fri 27 May 2016
SRR939940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661449
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.22254172279344286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.17748911858661817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.11595754170011596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.040.0404661
TCAACGC35550.033.8447344
ATCAACG35600.033.7972033
TATCAAC36450.033.4992942
CAACGCA36150.033.2829975
TACCTGG8100.032.3554152
AACGCAG37250.032.263526
GTACCTG9750.028.7169531
ACGCAGA42100.028.4053787
GGTATCA20500.028.1879181
CGCAGAG42500.028.1380338
GTGTAGG3450.027.6277791
AGTACTT23600.026.59665712-13
AGAGTAC35450.026.51718710-11
GTACTAG1608.651936E-626.0629271
GTACATG26400.025.4987951
TACATGG25350.025.3761162
GCAGAGT47250.025.0575089
GAGTACT23700.024.85269412-13
GCACCGT1451.3398704E-424.618976
Produced by FastQC (version 0.11.5)