FastQCFastQC Report
Fri 27 May 2016
SRR939939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191914
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22860.10429241293225922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG1300.02298.6108108-109
CCGCGCC4100.01700.5983108-109
CCGCTCC6700.01337.9973108-109
CCGTTCG804.5474735E-111245.0808108-109
CCTCGCG2800.01156.1465108-109
CTCCGCC2900.01132.2517110-111
CCGCACG704.2195097E-81067.2122108-109
CGTCGTC905.574293E-8995.009110-111
CGCGTCG703.498515E-5852.86487110-111
CCGCGCT1203.4924597E-10830.05396108-109
CTGCGCG1254.274625E-10796.85175108-109
CCGCGCG2600.0766.2036108-109
CGTCGCT706.028903E-5711.4748108-109
TTGTTCG4000.0684.7945108-109
CGTCGCC2950.0675.2981108-109
CCGCTAC1203.638288E-7622.54047108-109
CGTCGCG1003.4558798E-6597.0054110-111
CATCGCC3050.0571.5125108-109
CGGCGCG901.6736612E-6553.3693108-109
CGCGTCT1656.2848267E-7542.7322110-111
Produced by FastQC (version 0.11.5)