FastQCFastQC Report
Fri 27 May 2016
SRR939939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191914
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27930.1274228824671041No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA24390.11127261379780412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79350.038.070081
ATCAACG89000.033.1328353
TCAACGC89800.032.7713244
CAACGCA90050.032.6803445
TATCAAC93650.031.8693582
AACGCAG92950.031.683576
GGTATCA53850.031.2022691
GTACATG80300.030.2738321
TAGGACC21350.030.1348674
TACATGG80350.029.2856332
ACGCAGA103650.028.2980167
ACATGGG79400.027.9854133
CGCAGAG105150.027.8377518
CATGGGG35650.025.0654264
GAGTACT49700.023.64226312-13
GCAGAGT120900.023.6207289
GTAGGAC64650.023.2207053
GTCCTAC58150.022.3372421
AGTACTT50900.022.32512712-13
AGAGTAC96250.021.9744110-11
Produced by FastQC (version 0.11.5)