FastQCFastQC Report
Fri 27 May 2016
SRR939938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315930
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA21590.16406647770018162No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18110.1376213020449416No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA13820.10502078378029225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCT253.3105607E-103578.1907110-111
CCGATCG100.00129096042515.9153108-109
CGGTACG100.00129096042515.9153108-109
CCGCTCC3350.02027.7526108-109
CCGCTCG900.01956.8229108-109
CGACGCG304.5945726E-61677.2767108-109
CTCCGCC2400.01490.9126110-111
CGCGTCA250.00574002281192.7302110-111
CCGCGCC4250.01183.9601108-109
TTGTTCG1750.01006.3661108-109
CCGCACG502.1258571E-51006.3661108-109
CGGGACG300.008264715993.9417110-111
CGTCGAC300.008264715993.9417110-111
CGGCTCG705.829899E-5718.8329108-109
CCTCGCG2600.0677.3618108-109
CGCCGCT1352.8128306E-7662.62787110-111
CGTCGCC1903.6379788E-12662.08295108-109
CCGCGCG2300.0656.3257108-109
CGCCGCC2305.456968E-12648.22296110-111
CGGCGCT1001.01925776E-4596.3651110-111
Produced by FastQC (version 0.11.5)