##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939938_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315930 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42146238781698 33.0 31.0 34.0 29.0 34.0 2 31.57344235635634 34.0 31.0 34.0 29.0 34.0 3 31.675880935916044 34.0 31.0 34.0 29.0 34.0 4 34.97320526167805 37.0 35.0 37.0 32.0 37.0 5 34.98569604766211 37.0 35.0 37.0 32.0 37.0 6 35.15808819618065 37.0 35.0 37.0 33.0 37.0 7 35.141616955309175 37.0 36.0 37.0 33.0 37.0 8 35.16991709285448 37.0 36.0 37.0 33.0 37.0 9 36.82477031453041 39.0 38.0 39.0 33.0 39.0 10-11 36.78972057784229 39.0 38.0 39.0 33.0 39.0 12-13 36.730592812687604 39.0 38.0 39.0 33.0 39.0 14-15 38.20793203285889 41.0 39.0 41.0 33.5 41.0 16-17 38.18729339706519 41.0 39.0 41.0 33.0 41.0 18-19 38.178236684322115 41.0 39.0 41.0 33.5 41.0 20-21 38.21113433085347 41.0 39.0 41.0 34.0 41.0 22-23 38.18467205702431 41.0 39.0 41.0 33.0 41.0 24-25 38.19463649282257 41.0 39.0 41.0 33.0 41.0 26-27 38.15013906514784 41.0 39.0 41.0 33.0 41.0 28-29 38.08744538083333 41.0 39.0 41.0 33.0 41.0 30-31 38.0047779897107 40.0 38.0 41.0 33.0 41.0 32-33 37.91039037030845 40.0 38.0 41.0 33.0 41.0 34-35 37.857596528690735 40.0 38.0 41.0 33.0 41.0 36-37 38.03032570121511 41.0 38.5 41.0 33.0 41.0 38-39 37.99417332228918 41.0 38.5 41.0 33.0 41.0 40-41 37.90726216440085 41.0 38.0 41.0 33.0 41.0 42-43 37.84619356652709 41.0 38.0 41.0 33.0 41.0 44-45 37.756512504464524 40.5 38.0 41.0 32.5 41.0 46-47 37.653530962893164 40.0 38.0 41.0 32.0 41.0 48-49 37.563967308291474 40.0 38.0 41.0 32.0 41.0 50-51 37.44717120211561 40.0 38.0 41.0 31.5 41.0 52-53 37.3360003951578 40.0 38.0 41.0 31.0 41.0 54-55 37.1880764934305 40.0 37.5 41.0 31.0 41.0 56-57 37.00537110636584 40.0 37.0 41.0 31.0 41.0 58-59 36.83907122719293 40.0 37.0 41.0 30.5 41.0 60-61 36.621916059364864 40.0 36.0 41.0 30.5 41.0 62-63 36.37204980508082 39.5 36.0 41.0 30.0 41.0 64-65 36.08592516319257 39.0 35.0 41.0 30.0 41.0 66-67 35.79368849406883 39.0 35.0 41.0 29.0 41.0 68-69 35.411509730760756 38.5 35.0 40.5 29.0 41.0 70-71 34.999821799031864 37.5 35.0 40.0 28.5 41.0 72-73 34.61388903665089 37.0 34.5 39.5 28.0 41.0 74-75 34.1953116807125 36.5 34.0 39.0 28.0 41.0 76-77 33.72766180571915 36.0 34.0 39.0 26.5 40.5 78-79 33.2717260796547 35.5 34.0 37.5 26.0 39.5 80-81 32.83321871224153 35.0 34.0 37.0 26.0 39.0 82-83 32.620976419718374 35.0 34.0 37.0 26.0 39.0 84-85 32.174469766628924 35.0 34.0 36.0 25.0 38.0 86-87 31.486848464583982 35.0 34.0 36.0 10.0 37.0 88-89 16.044643712051553 18.0 16.5 18.5 2.0 19.0 90-91 2.770541746141512 2.0 2.0 2.0 2.0 2.0 92-93 2.2949491994255014 2.0 2.0 2.0 2.0 2.0 94-95 2.100023557484061 2.0 2.0 2.0 2.0 2.0 96-97 2.000032676510149 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.149695385279923 1101 2 0.2328057399705763 1101 3 0.2274590748607146 1101 4 0.25120330043269945 1101 5 0.23928391542334282 1101 6 0.2338871554297839 1101 7 0.1988544639988845 1101 8 0.30903122942739714 1101 9 0.35893401343213327 1101 10-11 0.24961955483021825 1101 12-13 0.3387800582034757 1101 14-15 0.4137097783052326 1101 16-17 0.43132241793747284 1101 18-19 0.40724707264589455 1101 20-21 0.41238512888649126 1101 22-23 0.3377966906974521 1101 24-25 0.390713421986689 1101 26-27 0.4579755004820143 1101 28-29 0.4249623095300734 1101 30-31 0.41835421720172405 1101 32-33 0.36897388195002634 1101 34-35 0.3435300119493476 1101 36-37 0.3912918538753303 1101 38-39 0.3660030167255144 1101 40-41 0.3937982427109503 1101 42-43 0.46458052969106944 1101 44-45 0.4619225959830189 1101 46-47 0.4003433596537107 1101 48-49 0.4510218626405518 1101 50-51 0.4046037344940814 1101 52-53 0.35437594678644047 1101 54-55 0.31569653987703816 1101 56-57 0.46152512526093403 1101 58-59 0.3734407760109022 1101 60-61 0.31567176221779647 1101 62-63 0.18597898331841378 1101 64-65 0.24229035144524147 1101 66-67 0.30858934733242904 1101 68-69 0.26215421639086145 1101 70-71 0.42099422951862664 1101 72-73 0.4396486232306458 1101 74-75 0.47080610062278794 1101 76-77 0.5301606475244185 1101 78-79 0.5288730459276607 1101 80-81 0.5371962965588821 1101 82-83 0.43652048969541823 1101 84-85 0.4975582193576287 1101 86-87 0.4638892303118176 1101 88-89 0.9001725196711128 1101 90-91 0.11184262149049484 1101 92-93 -0.09928863212889905 1101 94-95 0.05660910014900189 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.015746567474909767 1102 2 0.09669424653982972 1102 3 0.16852588202044672 1102 4 0.14526378314821642 1102 5 0.13022391781503728 1102 6 0.06198828787307775 1102 7 0.23401627545689507 1102 8 0.11349128254099838 1102 9 0.2147612791648399 1102 10-11 0.23511315705584224 1102 12-13 0.1793952643966037 1102 14-15 0.2963141158405733 1102 16-17 0.27379823590720065 1102 18-19 0.20571887212287976 1102 20-21 0.2424402280401523 1102 22-23 0.21819689286306243 1102 24-25 0.1939065555761843 1102 26-27 0.29978288584440804 1102 28-29 0.312672964515059 1102 30-31 0.27410758869682184 1102 32-33 0.29878507371177676 1102 34-35 0.2488802367909031 1102 36-37 0.34724693971674014 1102 38-39 0.31635962693172814 1102 40-41 0.2538397362996392 1102 42-43 0.2177754683306503 1102 44-45 0.30717931923048525 1102 46-47 0.33466774069076166 1102 48-49 0.340239452402308 1102 50-51 0.37599256258788216 1102 52-53 0.241354146294114 1102 54-55 0.30555808125442496 1102 56-57 0.21980381822417883 1102 58-59 0.2914074547864445 1102 60-61 0.15073587534028832 1102 62-63 0.11740400266629791 1102 64-65 0.16085523509848798 1102 66-67 0.06423407737590026 1102 68-69 0.23083744363989211 1102 70-71 0.16467655424261807 1102 72-73 0.1495742551674013 1102 74-75 0.15744150983891814 1102 76-77 0.16460359778852052 1102 78-79 0.16425151980294572 1102 80-81 0.09896341802296149 1102 82-83 0.08469338416090721 1102 84-85 0.17356696031262686 1102 86-87 0.28362339156145566 1102 88-89 1.0390742256690686 1102 90-91 0.1395797543038233 1102 92-93 0.004698892718743686 1102 94-95 0.11105815373868255 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.1485308260776641 1103 2 0.019035367380695334 1103 3 0.002606731599058776 1103 4 0.10351513979293969 1103 5 0.024376729031892808 1103 6 -0.08748550515217346 1103 7 0.0121717944054609 1103 8 -0.048455749958456806 1103 9 -0.0574024385641394 1103 10-11 -0.016138446352606195 1103 12-13 2.309041926977784E-4 1103 14-15 -0.01876342577023138 1103 16-17 0.008112899674948437 1103 18-19 0.07297443708954887 1103 20-21 0.109575190014084 1103 22-23 0.13079473690393684 1103 24-25 0.09318916229038621 1103 26-27 0.15272921139913365 1103 28-29 0.16066039512664787 1103 30-31 0.09905262263238512 1103 32-33 0.2071757073610101 1103 34-35 0.06837618297418402 1103 36-37 0.1937710169649023 1103 38-39 0.17290486805990213 1103 40-41 0.20056767150970956 1103 42-43 0.13610334396172874 1103 44-45 -0.0010894331454451844 1103 46-47 0.012353185854742321 1103 48-49 0.08670333930894003 1103 50-51 0.0939744132269098 1103 52-53 0.06364563650248556 1103 54-55 -0.034756053416323596 1103 56-57 -0.016259311051115333 1103 58-59 -0.008548861364566562 1103 60-61 0.03463298598158815 1103 62-63 -0.04873968051575872 1103 64-65 -0.024245071021930187 1103 66-67 0.008204816977922746 1103 68-69 0.02842254933702293 1103 70-71 0.003343652190032742 1103 72-73 0.04056637873600266 1103 74-75 -0.06981787885396784 1103 76-77 -0.05894877725677361 1103 78-79 -0.02560732467588167 1103 80-81 -0.020165105863100052 1103 82-83 0.007194845846946407 1103 84-85 -0.016601172486275573 1103 86-87 -0.07860297303083996 1103 88-89 0.6931523297233291 1103 90-91 -0.5405875003719043 1103 92-93 -0.3079332425878105 1103 94-95 -0.10907926698013348 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.11205187924336002 1104 2 -1.025982752551613E-4 1104 3 0.07148375934678697 1104 4 0.09194081081469108 1104 5 0.04839661947467988 1104 6 0.1123603288019197 1104 7 0.20110563039783358 1104 8 0.19052915083764077 1104 9 0.2462447543830919 1104 10-11 0.30999453373751606 1104 12-13 0.34781649981559326 1104 14-15 0.3558346953040896 1104 16-17 0.22140210999500454 1104 18-19 0.3020805115340366 1104 20-21 0.17679746981466593 1104 22-23 0.18111381095761914 1104 24-25 0.24048710004962004 1104 26-27 0.24470172709102656 1104 28-29 0.30491306766198534 1104 30-31 0.23275981942297364 1104 32-33 0.2271476595047588 1104 34-35 0.2769167983860328 1104 36-37 0.2636212988469353 1104 38-39 0.2641186989565938 1104 40-41 0.27452176068850775 1104 42-43 0.37193043184285557 1104 44-45 0.26578705264601155 1104 46-47 0.3371129221746685 1104 48-49 0.38738326771024845 1104 50-51 0.33798860341253345 1104 52-53 0.23436806852583913 1104 54-55 0.26754272642636323 1104 56-57 0.16515515162407723 1104 58-59 0.07760113312949812 1104 60-61 0.1218164219843203 1104 62-63 0.15999661133741228 1104 64-65 0.20919179539125565 1104 66-67 0.19206833900273068 1104 68-69 0.201391077008104 1104 70-71 0.16370683846105294 1104 72-73 0.25077307783367786 1104 74-75 0.18491317264915352 1104 76-77 0.12558868142176038 1104 78-79 0.19745248268133508 1104 80-81 0.17690913998639957 1104 82-83 0.09703813195883271 1104 84-85 0.2211959048364207 1104 86-87 0.3595754169487968 1104 88-89 0.7496577482064133 1104 90-91 -0.6170411199272414 1104 92-93 -0.3079332425878105 1104 94-95 -0.10907926698013348 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.09549623113049321 1105 2 0.08919857096804762 1105 3 0.020930744599727547 1105 4 0.2247131392889088 1105 5 0.22794398316194986 1105 6 0.2313497139734082 1105 7 0.22194391335810337 1105 8 0.126248345773746 1105 9 0.26647423322980046 1105 10-11 0.24871108133030617 1105 12-13 0.32593932551518634 1105 14-15 0.2964582634477253 1105 16-17 0.34235844155778494 1105 18-19 0.4048347667990271 1105 20-21 0.47114842523407674 1105 22-23 0.21992666414885775 1105 24-25 0.35254099724445354 1105 26-27 0.34360375841792745 1105 28-29 0.3570103660141939 1105 30-31 0.4060807291926878 1105 32-33 0.3590327673500653 1105 34-35 0.37449887027158724 1105 36-37 0.32647767198658073 1105 38-39 0.2935062250481977 1105 40-41 0.35790830251144 1105 42-43 0.3997498100565764 1105 44-45 0.3303510470798017 1105 46-47 0.37072866707938346 1105 48-49 0.4370405767536454 1105 50-51 0.39073766156923995 1105 52-53 0.41469765004195125 1105 54-55 0.4180472701838909 1105 56-57 0.412745390394079 1105 58-59 0.4411107509617338 1105 60-61 0.3003010300587192 1105 62-63 0.36713372590288884 1105 64-65 0.273578663889559 1105 66-67 0.3921278405894384 1105 68-69 0.3991898364901729 1105 70-71 0.3056048648326595 1105 72-73 0.3337348356017671 1105 74-75 0.31576363169169497 1105 76-77 0.4626588063220609 1105 78-79 0.3182852503430169 1105 80-81 0.19004668729069607 1105 82-83 0.2675085869285425 1105 84-85 0.22946620113523863 1105 86-87 0.15810686263583307 1105 88-89 0.9981903605912308 1105 90-91 -0.7782320850196438 1105 92-93 -0.3079332425878105 1105 94-95 -0.10907926698013348 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.10558505698866227 1106 2 -0.008015464823724727 1106 3 0.0724637146645648 1106 4 -0.02567719668220292 1106 5 -0.005452106998866668 1106 6 0.025800724980150846 1106 7 -0.11325723246347508 1106 8 0.07823702132774457 1106 9 0.05109368962294525 1106 10-11 -0.1701648764820689 1106 12-13 -0.09465643586464978 1106 14-15 -0.005334146790673344 1106 16-17 0.014524168700923212 1106 18-19 -0.07871677845068348 1106 20-21 -0.04159066207994755 1106 22-23 -0.02233454638383847 1106 24-25 -0.07850017039831414 1106 26-27 -0.15338694458394997 1106 28-29 0.006609631534615801 1106 30-31 0.07172335179058109 1106 32-33 0.06077362920353124 1106 34-35 -0.023860804208069908 1106 36-37 0.1250692750081086 1106 38-39 0.08849767109926887 1106 40-41 0.16152842043783977 1106 42-43 -0.22918620828659897 1106 44-45 0.04919706345824437 1106 46-47 -0.08306590019635252 1106 48-49 -0.04236972330863864 1106 50-51 -0.08854331030985207 1106 52-53 -0.017125103984724888 1106 54-55 0.04614552929591298 1106 56-57 -0.08453741141349269 1106 58-59 0.07294829281703841 1106 60-61 0.16842155451977447 1106 62-63 0.22840808662976286 1106 64-65 0.19739208434045707 1106 66-67 0.13614856758626104 1106 68-69 0.14272839144237537 1106 70-71 0.16732246851467636 1106 72-73 0.18140008305491762 1106 74-75 0.01462282648136437 1106 76-77 0.06411232446667725 1106 78-79 0.1274796742343014 1106 80-81 0.09190684459556309 1106 82-83 0.15934241353784984 1106 84-85 0.06433671096522176 1106 86-87 0.09059676719947163 1106 88-89 0.6407336633247311 1106 90-91 -0.20944098739698314 1106 92-93 -0.2780900458250386 1106 94-95 -0.10907926698013348 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.22463440335635454 1107 2 -0.2050006651453522 1107 3 -0.04437620940244713 1107 4 -0.03965089218849016 1107 5 -0.10151298236437611 1107 6 -0.16195104991319909 1107 7 -0.20114266594451635 1107 8 -0.1923203546312422 1107 9 -0.1958973547982552 1107 10-11 -0.1303820502755073 1107 12-13 -0.1647477196924143 1107 14-15 -0.16295820291821883 1107 16-17 -0.18714235690126912 1107 18-19 -0.17334836842865542 1107 20-21 -0.2145457814104148 1107 22-23 -0.11132066060172718 1107 24-25 -0.10048985455495796 1107 26-27 -0.14119053548085958 1107 28-29 -0.0692412391354651 1107 30-31 -0.15954416791310422 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-0.22469165079352393 2216 8 -0.35648269033600144 2216 9 -0.4978100032529156 2216 10-11 -0.47738675912474804 2216 12-13 -0.44816446052247727 2216 14-15 -0.45753307515559527 2216 16-17 -0.5894802188652832 2216 18-19 -0.5459201564916256 2216 20-21 -0.5427174626058857 2216 22-23 -0.6232282861033411 2216 24-25 -0.7435792549957867 2216 26-27 -0.7461739773364542 2216 28-29 -0.6916446928331297 2216 30-31 -0.7424615920091995 2216 32-33 -0.7581211184827694 2216 34-35 -0.735505525022127 2216 36-37 -0.7819795939628591 2216 38-39 -0.8508605671269649 2216 40-41 -0.9004145535775621 2216 42-43 -0.8954877792067393 2216 44-45 -0.8865478040587575 2216 46-47 -0.8820662529159691 2216 48-49 -0.7492979937976116 2216 50-51 -0.7774072483155408 2216 52-53 -0.9335665365993577 2216 54-55 -0.8764721636610844 2216 56-57 -0.8354749603306999 2216 58-59 -0.9436549691175031 2216 60-61 -0.8429534813194266 2216 62-63 -0.9248100845499536 2216 64-65 -1.011398793262174 2216 66-67 -1.0120600224084626 2216 68-69 -1.0401338125304065 2216 70-71 -1.1440856390861214 2216 72-73 -1.2245711461168085 2216 74-75 -1.2671356332317103 2216 76-77 -1.252792617022891 2216 78-79 -1.20560029227682 2216 80-81 -1.2378500172219944 2216 82-83 -1.1136165052836908 2216 84-85 -1.1470235881153457 2216 86-87 -1.1439109001549852 2216 88-89 -1.4120895948098795 2216 90-91 -0.7782320850196438 2216 92-93 -0.3079332425878105 2216 94-95 -0.10907926698013348 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20870.0 3 3092.0 4 2141.0 5 1628.0 6 1702.0 7 1773.0 8 2254.0 9 2549.0 10 2898.0 11 3430.0 12 3967.0 13 4593.0 14 5090.0 15 5864.0 16 6757.0 17 8227.0 18 10381.0 19 12602.0 20 13562.0 21 18218.0 22 22063.0 23 27821.0 24 41605.0 25 69453.0 26 133933.0 27 374458.0 28 496556.0 29 17808.0 30 635.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.36048270044759 20.04726695188954 14.657314598800847 23.93493574886202 2 13.507087035421497 20.146908180107665 43.99539791051763 22.350606873953208 3 18.845253299276287 25.508651097731555 29.089582193030466 26.556513409961685 4 11.65673415302229 17.495896613670453 39.398894765531104 31.448474467776162 5 12.888052609571595 38.48374957235717 35.03744250579693 13.5907553122743 6 30.148868100886826 37.228272020548204 19.60621005676594 13.016649821799032 7 28.03598975629403 31.66680598512079 22.420341507527 17.876862751058187 8 24.094746681054463 38.585031118676525 20.856504525316698 16.463717674952317 9 25.490641599477176 17.97436033831587 21.66399428541032 34.87100377679664 10-11 23.94914734525934 26.749888576010918 29.92671664186145 19.374247436868288 12-13 25.805127862185 24.227625162530238 29.220181638699938 20.747065336584825 14-15 20.846595505306325 28.625545669429858 25.917003912443636 24.610854912820177 16-17 20.778273837794707 30.04384748370918 28.917339514030054 20.26053916446606 18-19 21.534565805213106 28.212287530835862 29.83855155421868 20.414595109732353 20-21 22.320916633261202 28.19889771468631 28.22982705785573 21.250358594196758 22-23 21.33536688696831 29.731298867790894 28.30887521534315 20.624459029897647 24-25 21.774904440205788 28.341895085604857 29.4945399831298 20.388660491059554 26-27 20.4180693501934 29.674108805179607 28.919167432918165 20.98865441170883 28-29 20.57103080471929 29.440045109176594 27.879310154846216 22.1096139312579 30-31 21.342738595518 28.31829960560212 28.830180936675966 21.508780862203917 32-33 20.55206390375073 29.249120289927212 29.367288881931337 20.83152692439072 34-35 20.899631628642155 29.456713990702376 29.167222358374605 20.476432022280864 36-37 20.47445622954152 28.945249643312415 28.85820077473873 21.72209335240733 38-39 21.378226805377185 29.450844649791403 28.189455366166893 20.98147317866452 40-41 22.654776469873017 27.75934130234891 29.172638362222912 20.41324386555516 42-43 20.664777001816205 29.468056811532527 29.272339714118534 20.594826472532734 44-45 20.362770469672174 29.665061589592444 28.54247020259839 21.429697738136987 46-47 21.616537353810614 28.3048490421223 28.464583982430675 21.614029621636412 48-49 21.200695934467564 28.742557061420893 28.366776766366865 21.689970237744678 50-51 20.38147998274981 29.730133309015883 28.484623962946287 21.40376274528802 52-53 20.539694360642283 29.0974063969968 29.051165335542162 21.311733906818752 54-55 20.702471786645063 28.31738936652607 29.351654234843476 21.628484611985392 56-57 21.191552742167136 28.829952961023764 27.78909212496106 22.189402171848048 58-59 21.368233872622405 29.144749340770403 27.431892273905145 22.055124512702044 60-61 20.887813181552207 27.8842719597547 29.57763710835683 21.650277750336265 62-63 21.31180989870282 27.9285372322236 29.184873055557663 21.574779813515914 64-65 21.47925801524397 28.660719035207038 28.074631629341983 21.785391320207 66-67 21.360824663925893 29.704353575038184 27.466012629851132 21.46880913118479 68-69 21.930442163753067 27.921924210812687 29.130198381140588 21.017435244293658 70-71 22.420113531874797 27.905207723815096 28.542931614903527 21.13174712940658 72-73 21.671327502222763 28.894052115234093 28.017219760929535 21.41740062161361 74-75 20.9708723108372 29.310829603398357 28.953781736110585 20.764516349653857 76-77 21.317927245370193 28.201994027037912 29.31649099876133 21.16358772883056 78-79 21.12635384548161 28.173360614471544 28.796875207790702 21.903410332256147 80-81 21.445669602486454 28.919053445092064 28.581877455487753 21.05339949693373 82-83 21.063604477926685 28.83447074920503 29.303267710117968 20.798657062750323 84-85 21.01960706831716 28.450025970255982 29.28684955147639 21.243517409950467 86-87 20.23464979024157 29.680143565122002 28.12526003700077 21.959946607635654 88-89 30.173826143786552 17.94754817136513 34.87357351163391 17.005052173214413 90-91 30.855640436838566 14.542372846418013 16.75485754098969 37.84712917575373 92-93 22.821992287212666 17.129952684868982 35.4309197811436 24.61713524677475 94-95 16.103964836433743 6.969273119048191 27.579549320750697 49.34721272376737 96-97 27.341273780963967 7.2949528566792665 14.681406579467945 50.68236678288882 98-99 57.264518833529145 14.097154424459044 18.578149907059608 10.060176834952205 100-101 30.9188309014072 7.318270808649759 12.232387807211511 49.53051048273153 102-103 24.01497328644399 2.1534696645846885 36.58395817131566 37.24759887765566 104-105 42.82915817621259 5.179871374355168 38.79313087545735 13.197839573974896 106-107 6.079440553670335 1.8149000030942826 6.661165719472321 85.44449372376306 108-109 18.188611788682387 10.632133064903527 21.48632499105548 49.6929301553586 110-111 40.59340036469902 2.7101338845666683 24.576599846612613 32.119865904121696 112-113 28.412508306110514 2.9070392048416167 38.11364748668038 30.56680500236749 114-115 30.02868123673493 4.242241725463202 22.874720357941836 42.85435667986003 116-117 20.654502483076634 4.358284312558051 20.059108451306408 54.9281047530589 118-119 21.0172765759224 4.371891654906904 14.983490662976612 59.62734110619409 120-121 26.517345126186253 4.436748931955161 28.85992672364206 40.18597921821653 122-123 35.057503124222904 1.7221635303006064 33.26153032278093 29.958803022695555 124-125 39.26987003845481 1.9607603624539929 19.17373682004567 39.59563277904552 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 3.5 2 11.5 3 28.5 4 40.0 5 42.0 6 44.0 7 44.0 8 42.0 9 47.5 10 57.0 11 58.5 12 59.0 13 71.5 14 93.0 15 119.0 16 161.5 17 231.5 18 342.0 19 529.5 20 790.0 21 1130.0 22 1689.5 23 2601.5 24 3768.0 25 5307.0 26 7274.5 27 9953.0 28 13430.5 29 17624.0 30 22756.0 31 28784.0 32 36276.0 33 44721.5 34 52641.0 35 58419.0 36 61128.5 37 61635.0 38 60670.0 39 58865.5 40 57101.5 41 55061.5 42 52876.5 43 50914.5 44 49105.0 45 47351.0 46 45625.5 47 44024.5 48 42180.5 49 40213.0 50 38030.0 51 35326.5 52 32265.5 53 28964.5 54 25498.5 55 22194.0 56 19177.5 57 16313.0 58 13665.5 59 11209.0 60 8952.0 61 7057.5 62 5535.5 63 4292.5 64 3232.5 65 2341.0 66 1692.5 67 1253.0 68 885.5 69 620.0 70 428.5 71 291.0 72 200.5 73 141.5 74 107.0 75 86.0 76 57.0 77 33.5 78 16.5 79 12.0 80 15.5 81 16.0 82 10.5 83 4.5 84 6.5 85 9.0 86 6.0 87 4.0 88 3.5 89 3.5 90 3.0 91 2.5 92 2.0 93 2.0 94 2.0 95 1.5 96 2.5 97 3.5 98 5.5 99 3.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 4.5595130440069005E-4 3 0.03723602319272302 4 0.04202351188893026 5 0.04407529275873337 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.979147827012075E-4 12-13 0.001595829565402415 14-15 3.799594203339083E-5 16-17 3.7995942033390833E-4 18-19 0.006991253334143913 20-21 0.002089776811836496 22-23 5.319431884674717E-4 24-25 0.0 26-27 0.0 28-29 8.739066667679892E-4 30-31 0.0 32-33 0.0010258904349015525 34-35 1.8997971016695417E-4 36-37 1.8997971016695417E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.1398782610017251E-4 46-47 0.0 48-49 1.1398782610017251E-4 50-51 1.8997971016695417E-4 52-53 0.0 54-55 0.0014818417393022425 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.6597159423373583E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.8997971016695417E-4 80-81 0.0 82-83 6.459310145676442E-4 84-85 1.1398782610017251E-4 86-87 8.739066667679892E-4 88-89 19.12977893960925 90-91 26.269330435509485 92-93 40.43049402323832 94-95 44.71913399648918 96-97 48.71634509434392 98-99 93.11137370528829 100-101 52.02449978342313 102-103 53.389693980682864 104-105 74.9205884811502 106-107 81.5808971601833 108-109 12.598276504069366 110-111 38.864605260158214 112-113 52.25410926113091 114-115 86.75233485063795 116-117 66.496356189159 118-119 72.26714186924836 120-121 80.51366714034941 122-123 76.01099602562446 124-125 50.23105332350505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1315930.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.86771399428834 #Duplication Level Percentage of deduplicated Percentage of total 1 86.04996595870095 63.56314274655569 2 9.43194835000109 13.934329262535607 3 2.226870999540771 4.93481610288758 4 0.8567180579888909 2.5313521792506206 5 0.380787456613316 1.406394946886245 6 0.24120834422572154 1.0690505390580871 7 0.15395758059472742 0.7960746171436744 8 0.10889040847971107 0.6434788440240421 9 0.0810005151026469 0.538499059469312 >10 0.4174600472281135 5.633975054176389 >50 0.032663814146944595 1.6474146015351816 >100 0.017190322014268553 2.3961971480941964 >500 8.234740694531176E-4 0.3344027383748057 >1k 5.146712934081985E-4 0.5708721600086497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2159 0.16406647770018162 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1811 0.1376213020449416 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1382 0.10502078378029225 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.599188406678167E-5 0.0 4 0.0 0.0 0.0 7.599188406678167E-5 0.0 5 0.0 0.0 0.0 7.599188406678167E-5 0.0 6 0.0 0.0 0.0 7.599188406678167E-5 0.0 7 0.0 0.0 0.0 7.599188406678167E-5 0.0 8 7.599188406678167E-5 0.0 0.0 7.599188406678167E-5 0.0 9 7.599188406678167E-5 0.0 0.0 7.599188406678167E-5 0.0 10-11 7.599188406678167E-5 0.0 0.0 1.8997971016695417E-4 0.0 12-13 7.599188406678167E-5 0.0 0.0 2.6597159423373583E-4 0.0 14-15 7.599188406678167E-5 0.0 0.0 3.0396753626712666E-4 0.0 16-17 7.599188406678167E-5 0.0 0.0 3.0396753626712666E-4 0.0 18-19 7.599188406678167E-5 0.0 0.0 3.0396753626712666E-4 0.0 20-21 7.599188406678167E-5 0.0 0.0 3.7995942033390833E-4 0.0 22-23 7.599188406678167E-5 0.0 0.0 3.7995942033390833E-4 0.0 24-25 7.599188406678167E-5 0.0 0.0 5.699391305008625E-4 0.0 26-27 7.599188406678167E-5 0.0 0.0 7.599188406678167E-4 0.0 28-29 7.599188406678167E-5 7.599188406678167E-5 0.0 0.0014438457972688517 0.0 30-31 7.599188406678167E-5 7.599188406678167E-5 0.0 0.002963683478604485 0.0 32-33 7.599188406678167E-5 7.599188406678167E-5 0.0 0.007371212754477822 0.0 34-35 7.599188406678167E-5 7.599188406678167E-5 0.0 0.012614652755085757 0.0 36-37 7.599188406678167E-5 1.139878261001725E-4 0.0 0.022379609857667203 0.0 38-39 7.599188406678167E-5 1.5198376813356333E-4 0.0 0.040275698555394285 0.0 40-41 7.599188406678167E-5 1.5198376813356333E-4 0.0 0.0617814017462935 0.0 42-43 7.599188406678167E-5 1.5198376813356333E-4 0.0 0.08283115363279202 0.0 44-45 1.5198376813356333E-4 1.5198376813356333E-4 0.0 0.10650262551959451 0.0 46-47 1.5198376813356333E-4 1.8997971016695417E-4 0.0 0.13366972407346894 0.0 48-49 1.5198376813356333E-4 2.27975652200345E-4 0.0 0.16813204349775443 0.0 50-51 1.5198376813356333E-4 2.27975652200345E-4 0.0 0.20514009103827713 0.0 52-53 1.5198376813356333E-4 2.27975652200345E-4 0.0 0.2432880168398015 0.0 54-55 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.2858434719171993 0.0 56-57 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.33379435076333847 0.0 58-59 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.38744462091448634 0.0 60-61 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.4494919942550135 0.0 62-63 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.5183786371615511 0.0 64-65 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.5885191461551906 0.0 66-67 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.6693365148602128 0.0 68-69 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.7659221995090923 0.0 70-71 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.8760724354638925 0.0 72-73 1.5198376813356333E-4 3.0396753626712666E-4 0.0 0.9822330975051865 0.0 74-75 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.093713191431155 0.0 76-77 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.2105887091258654 0.0 78-79 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243227223332548 0.0 80-81 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 82-83 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 84-85 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 86-87 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 88-89 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 90-91 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 92-93 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 94-95 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 96-97 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 98-99 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 100-101 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 102-103 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 104-105 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 106-107 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 108-109 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 110-111 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 112-113 1.5198376813356333E-4 3.0396753626712666E-4 0.0 1.243607182752882 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGCT 25 3.3105607E-10 3578.1907 110-111 CCGATCG 10 0.0012909604 2515.9153 108-109 CGGTACG 10 0.0012909604 2515.9153 108-109 CCGCTCC 335 0.0 2027.7526 108-109 CCGCTCG 90 0.0 1956.8229 108-109 CGACGCG 30 4.5945726E-6 1677.2767 108-109 CTCCGCC 240 0.0 1490.9126 110-111 CGCGTCA 25 0.0057400228 1192.7302 110-111 CCGCGCC 425 0.0 1183.9601 108-109 TTGTTCG 175 0.0 1006.3661 108-109 CCGCACG 50 2.1258571E-5 1006.3661 108-109 CGGGACG 30 0.008264715 993.9417 110-111 CGTCGAC 30 0.008264715 993.9417 110-111 CGGCTCG 70 5.829899E-5 718.8329 108-109 CCTCGCG 260 0.0 677.3618 108-109 CGCCGCT 135 2.8128306E-7 662.62787 110-111 CGTCGCC 190 3.6379788E-12 662.08295 108-109 CCGCGCG 230 0.0 656.3257 108-109 CGCCGCC 230 5.456968E-12 648.22296 110-111 CGGCGCT 100 1.01925776E-4 596.3651 110-111 >>END_MODULE