FastQCFastQC Report
Fri 27 May 2016
SRR939938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315930
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.17964481393387188No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22310.1695378933529899No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC17850.13564551305920527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.11847134726011262No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA13850.10524875943249261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45850.045.083831
ATCAACG54450.037.958823
TCAACGC54700.037.785334
GGTATCA28650.037.426441
CAACGCA55250.037.4091875
TATCAAC56000.037.2272642
AACGCAG56650.036.5467346
GTACATG49650.033.3546371
TACATGG48900.033.2533152
ACATGGG48300.032.309873
ACGCAGA64800.031.7665737
CGCAGAG64750.031.6992198
CGATCGT1350.030.84846324-25
TCGCGCT1350.030.84846360-61
CATGGGG26900.028.7853954
CCCGCGA1450.028.72425720-21
TAGGACC21550.027.6397344
GCAGAGT74150.027.6004759
CGCGATC1550.026.86852622-23
TAGACGT1358.187334E-526.4727235
Produced by FastQC (version 0.11.5)