FastQCFastQC Report
Fri 27 May 2016
SRR939937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563959
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34250.6073136522335844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20570.3647428270494841No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19840.3517986236588121No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15610.2767931711347811No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9930.17607662968407278No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9910.17572199397473928No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9690.171821001172071No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC9530.16898391549740316No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT9260.1641963334214012No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7920.14043574089605804No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7610.13493888740138912No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7540.1336976624187219No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6870.12181736615605035No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5820.10319899141604265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT109.0402015E-43000.5808110-111
CGTCGTC109.0402015E-43000.5808110-111
CGCGTCG109.0402015E-43000.5808110-111
CCGCTAG100.00138269942427.6055108-109
CCGCGAC100.00138269942427.6055108-109
CCGTACC150.00311065071618.4037108-109
CGTCGCG150.00311065071618.4037108-109
GGTCGCC800.01517.2534108-109
CCGCGCC1450.01506.7897108-109
CCGCTCC1350.01258.7583108-109
CGTCGGC200.00552929431213.8027108-109
CGGGTTT250.0056482671200.2323110-111
CGTCGCT300.0081326121000.1936110-111
CCGCTCG250.0086383475971.04224108-109
CTCCGCC1453.3651304E-10827.74646110-111
TTGTTCG1203.8198777E-10809.20184108-109
CCGCACC901.2474811E-7809.20184108-109
CGTCGCC1254.674803E-10776.8338108-109
CCGTTGG655.1527702E-5746.9555108-109
CCTCGCG706.434706E-5693.6016108-109
Produced by FastQC (version 0.11.5)