FastQCFastQC Report
Fri 27 May 2016
SRR939937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563959
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.7734604820563197No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34600.6135197771469203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.6117465986002528No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20170.35765011286281445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20150.35729547715348103No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16470.29204250663612075No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10530.1867157009640772No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10130.17962298677740757No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9960.176608583248073No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9170.16260047272940056No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT8920.15816752636273204No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8590.15231603715872963No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8480.15036554075739547No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.14770577293739437No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7930.1406130587507248No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7340.13015130532538713No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.12625031252271884No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6180.10958243418404529No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGC255.3348136E-447.5959252-53
GTAATCG350.00279013633.99708630-31
AACGTGC400.005364407329.74745416-17
GTTGCGG800.005362160529.7474548
GTACATG32650.029.0064491
GAGTACT39950.028.07206212-13
TACATGG33100.027.707432
AGTACTT39750.027.68945112-13
ACATGGG33100.026.627923
CTGTCGA450.00953286926.4421850-51
GTACTTT43400.025.42927214-15
GTATAAG2651.3569661E-924.7244951
TAGGACC7150.024.1543564
TAGGACG13100.024.0939564
AGAGTAC63850.023.94704810-11
TGTAGGA19200.023.8832262
GTAGGAC15250.023.8211923
AGGACGT13300.023.731645
TACTTTT46850.023.23920414-15
CATGGGA22200.023.0700744
Produced by FastQC (version 0.11.5)