##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939937_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 563959 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76133194079711 34.0 31.0 34.0 31.0 34.0 2 32.818899955493215 34.0 31.0 34.0 31.0 34.0 3 32.84521392512576 34.0 31.0 34.0 31.0 34.0 4 36.295147342271335 37.0 37.0 37.0 35.0 37.0 5 36.21706719814738 37.0 37.0 37.0 35.0 37.0 6 36.36692206348334 37.0 37.0 37.0 35.0 37.0 7 36.350007713326676 37.0 37.0 37.0 35.0 37.0 8 36.37336756750047 37.0 37.0 37.0 35.0 37.0 9 38.19011133788094 39.0 39.0 39.0 37.0 39.0 10-11 38.18152560735798 39.0 39.0 39.0 37.0 39.0 12-13 38.1776051095913 39.0 39.0 39.0 37.0 39.0 14-15 39.62436187737052 41.0 40.0 41.0 37.0 41.0 16-17 39.656160288247904 41.0 39.5 41.0 37.0 41.0 18-19 39.74333151878062 41.0 40.0 41.0 37.0 41.0 20-21 39.762134304089486 41.0 40.0 41.0 37.0 41.0 22-23 39.695906971960724 41.0 40.0 41.0 37.0 41.0 24-25 39.643293218124015 41.0 40.0 41.0 37.0 41.0 26-27 39.51657567305425 41.0 40.0 41.0 36.0 41.0 28-29 39.41606482031495 41.0 39.0 41.0 36.0 41.0 30-31 39.21017396654722 41.0 39.0 41.0 35.5 41.0 32-33 38.945705272901044 41.0 38.5 41.0 35.0 41.0 34-35 38.77472298518155 41.0 38.0 41.0 35.0 41.0 36-37 38.71133983144165 41.0 38.0 41.0 35.0 41.0 38-39 38.705535331469136 41.0 39.0 41.0 35.0 41.0 40-41 38.589533104356875 41.0 39.0 41.0 35.0 41.0 42-43 38.41124088807874 41.0 38.5 41.0 35.0 41.0 44-45 38.12997842041709 41.0 38.0 41.0 34.5 41.0 46-47 37.71983778962655 41.0 38.0 41.0 33.5 41.0 48-49 37.31695389203825 41.0 38.0 41.0 33.0 41.0 50-51 37.08928663253889 41.0 38.0 41.0 32.0 41.0 52-53 36.983324142357866 41.0 38.0 41.0 32.0 41.0 54-55 36.86208394581875 40.5 37.5 41.0 31.5 41.0 56-57 36.716118547624916 40.0 37.0 41.0 31.0 41.0 58-59 36.55333189114812 40.0 37.0 41.0 31.0 41.0 60-61 36.331569316209155 40.0 36.0 41.0 31.0 41.0 62-63 36.121396236251215 39.5 36.0 41.0 30.0 41.0 64-65 35.86406547284466 39.0 35.0 41.0 30.0 41.0 66-67 35.555720362650476 39.0 35.0 41.0 29.0 41.0 68-69 35.19837257672987 38.5 35.0 41.0 29.0 41.0 70-71 34.791737519926095 37.0 35.0 40.0 28.5 41.0 72-73 34.42469842665868 37.0 35.0 39.0 28.0 41.0 74-75 34.0671768692405 36.0 35.0 39.0 28.0 41.0 76-77 33.69512234045383 36.0 35.0 39.0 27.5 40.5 78-79 33.322268994731886 35.5 34.5 37.0 26.5 39.0 80-81 33.03557084823542 35.0 34.0 37.0 26.5 39.0 82-83 32.79694268554984 35.0 34.0 37.0 26.5 39.0 84-85 32.534940483262076 35.0 34.0 36.0 26.0 37.0 86-87 32.337274518183065 35.0 34.0 36.0 26.0 37.0 88-89 32.132291531831214 35.0 34.0 35.5 26.0 37.0 90-91 31.964479687353162 35.0 34.0 35.0 25.5 36.0 92-93 31.826898763917235 35.0 34.0 35.0 25.0 36.0 94-95 31.725785917061344 35.0 34.0 35.0 25.0 36.0 96-97 31.638386833085384 35.0 34.0 35.0 25.0 36.0 98-99 31.55877466269711 35.0 34.0 35.0 25.0 35.5 100-101 31.510880223562353 35.0 34.0 35.0 24.5 35.0 102-103 30.658937972441258 34.5 32.0 35.0 21.5 35.0 104-105 31.318188910896005 35.0 34.0 35.0 24.0 35.0 106-107 31.364628457033223 35.0 34.0 35.0 24.0 35.0 108-109 31.357948006858656 35.0 34.0 35.0 23.0 35.0 110-111 31.32471686771556 35.0 34.0 35.0 23.0 35.0 112-113 31.220176466728965 35.0 34.0 35.0 19.5 35.0 114-115 31.13694967187331 35.0 34.0 35.0 19.0 35.0 116-117 31.050267838619476 35.0 34.0 35.0 17.5 35.0 118-119 30.950231311141415 35.0 34.0 35.0 11.0 35.0 120-121 30.82786603281444 35.0 34.0 35.0 2.0 35.0 122-123 30.631130099883144 35.0 34.0 35.0 2.0 35.0 124-125 28.62423154874734 33.0 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12953066206050323 1101 2 0.1899665079602002 1101 3 0.1932669476826021 1101 4 0.12216527255159093 1101 5 0.13125579122605302 1101 6 0.08357277279505126 1101 7 0.12152963492743396 1101 8 0.1719551256974725 1101 9 0.16883448936271606 1101 10-11 0.24959386505852166 1101 12-13 0.167365345256151 1101 14-15 0.2306330231137892 1101 16-17 0.32012883332961906 1101 18-19 0.3337946689607989 1101 20-21 0.3447633566501551 1101 22-23 0.3506489057660218 1101 24-25 0.2574635309288027 1101 26-27 0.31205468118878343 1101 28-29 0.3611774619598691 1101 30-31 0.31756269943327453 1101 32-33 0.41498981321361583 1101 34-35 0.36773944280613335 1101 36-37 0.34342945391622237 1101 38-39 0.30715057790758493 1101 40-41 0.4906713106341414 1101 42-43 0.3987474584152366 1101 44-45 0.5862683059121139 1101 46-47 0.5110389350555025 1101 48-49 0.4025575259938847 1101 50-51 0.5115167491773462 1101 52-53 0.4699366439278947 1101 54-55 0.43561360003372585 1101 56-57 0.4125269095148809 1101 58-59 0.4094445700196303 1101 60-61 0.4775182626132164 1101 62-63 0.5490143910860397 1101 64-65 0.45038636950422983 1101 66-67 0.5223662713000365 1101 68-69 0.4267779758309729 1101 70-71 0.5058745617802245 1101 72-73 0.40838476566815984 1101 74-75 0.3961065330004132 1101 76-77 0.2923826074384195 1101 78-79 0.2715461953304654 1101 80-81 0.2771303982132025 1101 82-83 0.32154685798027316 1101 84-85 0.32135119084636443 1101 86-87 0.38858943128537504 1101 88-89 0.3657336479635873 1101 90-91 0.37714694699736384 1101 92-93 0.3733845626613359 1101 94-95 0.39007043915766104 1101 96-97 0.3348247667414057 1101 98-99 0.4114533280060968 1101 100-101 0.3875750983392692 1101 102-103 0.472348439010819 1101 104-105 0.3217368063996062 1101 106-107 0.32980149532938796 1101 108-109 0.47383933099930786 1101 110-111 0.4399623079369306 1101 112-113 0.47344851338936067 1101 114-115 0.4796998189092001 1101 116-117 0.5682853208706149 1101 118-119 0.5166270778131938 1101 120-121 0.4718105070644114 1101 122-123 0.5634073038866205 1101 124-125 0.7538347389503457 1102 1 -0.08078342088045787 1102 2 0.10843775591824567 1102 3 0.06066539923174474 1102 4 -0.05096985998647341 1102 5 -0.1763323730085773 1102 6 -0.09076350735374916 1102 7 -0.049798628419033264 1102 8 0.01497714444910514 1102 9 0.03461482348766509 1102 10-11 0.131936308810495 1102 12-13 0.010528927833334478 1102 14-15 0.1326245258524139 1102 16-17 0.170879378993817 1102 18-19 -0.004518851156703363 1102 20-21 0.055206133977982574 1102 22-23 0.07137693849214344 1102 24-25 0.03791519674894772 1102 26-27 -0.029253986292374634 1102 28-29 0.08919560272065041 1102 30-31 0.1140625552955612 1102 32-33 0.14146619521974202 1102 34-35 0.1498975961961193 1102 36-37 0.1962022173793727 1102 38-39 0.18442761059252177 1102 40-41 0.2601547828430171 1102 42-43 0.37908724591506626 1102 44-45 0.32504443945227024 1102 46-47 0.44927849879869797 1102 48-49 0.675633287521471 1102 50-51 0.6881746760652092 1102 52-53 0.6683662292231034 1102 54-55 0.6995005823391125 1102 56-57 0.7485719765732384 1102 58-59 0.7744553117111224 1102 60-61 0.8301297291114906 1102 62-63 0.9016207098088458 1102 64-65 0.9942797350512009 1102 66-67 0.869599170389904 1102 68-69 0.7866846981393678 1102 70-71 0.811686743476109 1102 72-73 0.9874580288087103 1102 74-75 0.9270326521267975 1102 76-77 0.9209259928901119 1102 78-79 0.9491495969804973 1102 80-81 0.933050511601536 1102 82-83 0.8389068728058575 1102 84-85 0.8244175491040906 1102 86-87 0.7239953162223003 1102 88-89 0.689059419786723 1102 90-91 0.7645556597789422 1102 92-93 0.7012721215175688 1102 94-95 0.7731095485238697 1102 96-97 0.7404729059921706 1102 98-99 0.6569421607651655 1102 100-101 0.8531647931791397 1102 102-103 0.6261571064919806 1102 104-105 0.7383239685339404 1102 106-107 0.7774048626609193 1102 108-109 0.7507656285819131 1102 110-111 0.6745104018872681 1102 112-113 0.757199045326157 1102 114-115 0.7640474928010583 1102 116-117 0.8593988876612855 1102 118-119 0.759602938178066 1102 120-121 0.6677211142273691 1102 122-123 0.620781835439054 1102 124-125 0.6590907893024536 1103 1 0.03491386588161305 1103 2 0.05120459184172432 1103 3 0.022986525558870596 1103 4 -0.00961093297205906 1103 5 -0.0906589360695591 1103 6 0.058791552399029 1103 7 -0.005753390690152571 1103 8 0.07085112506150892 1103 9 0.1123575531383807 1103 10-11 0.05879685145807656 1103 12-13 0.06978483899915489 1103 14-15 0.07056369598114287 1103 16-17 0.03958994985087827 1103 18-19 0.04306539257309794 1103 20-21 0.03043950321427502 1103 22-23 0.040231121186920404 1103 24-25 0.14056881012536593 1103 26-27 0.16004757292185445 1103 28-29 0.09823866062698983 1103 30-31 0.01422668311009545 1103 32-33 0.08834904899210727 1103 34-35 0.28065489219037687 1103 36-37 0.2425010069367417 1103 38-39 0.23501872717456251 1103 40-41 0.07402037754989976 1103 42-43 0.1954596365392689 1103 44-45 0.08372526214294851 1103 46-47 0.07882086429082591 1103 48-49 -0.0748026769858896 1103 50-51 -0.04646398629310511 1103 52-53 -6.597747775103358E-4 1103 54-55 -0.06415536472967176 1103 56-57 -0.0027038607350675647 1103 58-59 -0.18116927155809748 1103 60-61 -0.19366559837479258 1103 62-63 -0.19635910759895836 1103 64-65 -0.2870391858084176 1103 66-67 -0.3256936951791545 1103 68-69 -0.22970243834316761 1103 70-71 -0.07659094027010127 1103 72-73 -0.03285278824005644 1103 74-75 -0.028498968122462998 1103 76-77 0.03309068489401312 1103 78-79 -0.09124123237786108 1103 80-81 -0.1395023170387617 1103 82-83 -0.09447390372385911 1103 84-85 -0.08585710853554929 1103 86-87 -0.03205369131732283 1103 88-89 -0.08422941952188268 1103 90-91 -0.04681596149612233 1103 92-93 -0.024980635316818933 1103 94-95 0.0051516575662873265 1103 96-97 0.0818395264978804 1103 98-99 0.06575505505659507 1103 100-101 0.04592201223848491 1103 102-103 0.19960430349664549 1103 104-105 0.18068689616383082 1103 106-107 0.20176822625531443 1103 108-109 0.23162165771460153 1103 110-111 0.09441474614535394 1103 112-113 0.03469686550362283 1103 114-115 0.05241878069150019 1103 116-117 0.13785839425204216 1103 118-119 0.07715499746923982 1103 120-121 0.09887077827825053 1103 122-123 0.16665107173181326 1103 124-125 0.10131587521310692 1104 1 0.0693117021367442 1104 2 0.18056092737106155 1104 3 0.02140465932892255 1104 4 0.021018339617612014 1104 5 0.06052715343304982 1104 6 0.07107738011828957 1104 7 0.03751530324664998 1104 8 0.023348612685730075 1104 9 -0.024027231169505114 1104 10-11 -0.013544062426362302 1104 12-13 0.10282878386335881 1104 14-15 0.09600537784613294 1104 16-17 0.17722626949951348 1104 18-19 0.26007417658321685 1104 20-21 0.22069768859658723 1104 22-23 0.2010495353505135 1104 24-25 0.15876802601598428 1104 26-27 0.16237229594018743 1104 28-29 0.23352389282014485 1104 30-31 0.22111772664553797 1104 32-33 0.21065212215497553 1104 34-35 0.1748063295174518 1104 36-37 -0.025082521803604152 1104 38-39 -0.0700007514919534 1104 40-41 0.09124876181311947 1104 42-43 0.07290842749719673 1104 44-45 0.13411390496375475 1104 46-47 0.3731720510006653 1104 48-49 0.1564351488475353 1104 50-51 0.22607605663440467 1104 52-53 0.14265730565573165 1104 54-55 0.03818672780019483 1104 56-57 0.0788770129513594 1104 58-59 0.0017720070860889336 1104 60-61 0.06599464157260826 1104 62-63 0.21306664394258235 1104 64-65 0.2057292041619263 1104 66-67 0.25094927451105065 1104 68-69 0.14740981832859035 1104 70-71 0.29741186366533157 1104 72-73 0.18880389095746608 1104 74-75 0.061364991381758216 1104 76-77 -0.03179529666949321 1104 78-79 0.2017622663380365 1104 80-81 0.22933460806142136 1104 82-83 0.21843164335371767 1104 84-85 0.21707957959487345 1104 86-87 0.3091641595778256 1104 88-89 0.29812470759812726 1104 90-91 0.25231339173770806 1104 92-93 0.2358024194839956 1104 94-95 0.2271355659545229 1104 96-97 0.2052452534739757 1104 98-99 0.15711248238379838 1104 100-101 0.22257149549551514 1104 102-103 0.25856449009482674 1104 104-105 0.3639988277775821 1104 106-107 0.4020883215394946 1104 108-109 0.35032986232744534 1104 110-111 0.3704348947787466 1104 112-113 0.36143908954550596 1104 114-115 0.3870486239936959 1104 116-117 0.3284903343024155 1104 118-119 0.379620227429168 1104 120-121 0.22711515489888967 1104 122-123 0.3238389478127566 1104 124-125 0.13799501070770503 1105 1 -0.07466785514289853 1105 2 -0.009574844310847652 1105 3 -0.08219128907168027 1105 4 0.009285326471463407 1105 5 -0.052083295752623826 1105 6 0.04328574808725705 1105 7 -0.05032681984069143 1105 8 0.00330920503202492 1105 9 -0.010924186312514905 1105 10-11 0.18910825739137493 1105 12-13 0.1197802324025048 1105 14-15 0.10486902120687347 1105 16-17 0.1634751534624428 1105 18-19 0.1194565847603073 1105 20-21 0.07703062169593977 1105 22-23 0.08723222677011222 1105 24-25 0.04700422564037865 1105 26-27 0.13524104998100483 1105 28-29 0.09577413142116598 1105 30-31 0.0817824229295141 1105 32-33 0.10037226623921214 1105 34-35 0.298768030204009 1105 36-37 0.24966887131853355 1105 38-39 0.1712864625716506 1105 40-41 0.15556174478778217 1105 42-43 0.1742969315457117 1105 44-45 0.04022517001101278 1105 46-47 0.08473573438480031 1105 48-49 0.06734768232865207 1105 50-51 -0.0479841631864133 1105 52-53 -0.15105594208910134 1105 54-55 -0.18234681488629434 1105 56-57 -0.05614038183328063 1105 58-59 -0.08383188441972322 1105 60-61 0.0912201580283778 1105 62-63 0.1689762526368952 1105 64-65 0.046787053366081466 1105 66-67 0.04541373065463716 1105 68-69 0.2612455992466636 1105 70-71 0.3501365334722948 1105 72-73 0.37849786589666223 1105 74-75 0.2872878385847386 1105 76-77 0.20545571161227372 1105 78-79 0.3020101035351601 1105 80-81 0.235903063466111 1105 82-83 0.24706838483334792 1105 84-85 0.18776551906717742 1105 86-87 0.07045229725832058 1105 88-89 0.27880264239077235 1105 90-91 0.2516961077870903 1105 92-93 0.06706377227485305 1105 94-95 0.0019500728970456294 1105 96-97 0.14984671278865136 1105 98-99 0.01846373388467626 1105 100-101 0.1600965101194518 1105 102-103 0.08990281095933028 1105 104-105 0.1275405906543412 1105 106-107 0.05242236297910452 1105 108-109 -0.015430120431705774 1105 110-111 -0.18781300399284362 1105 112-113 -0.1086880617121544 1105 114-115 0.10165408564819955 1105 116-117 -0.02530265236671525 1105 118-119 0.17686938847716505 1105 120-121 -0.1647785675850244 1105 122-123 -0.12926748363947738 1105 124-125 0.16026649249705827 1106 1 0.06998672851877785 1106 2 0.0847513968239042 1106 3 0.054478004335202 1106 4 0.0839372833929275 1106 5 0.022489858962373432 1106 6 0.10735194194068498 1106 7 0.13404407587771772 1106 8 -0.021303350786205044 1106 9 0.0544291102464598 1106 10-11 0.10296431202757361 1106 12-13 -0.054950526725420445 1106 14-15 0.014430355590896227 1106 16-17 0.14766217736578824 1106 18-19 0.055048914678877736 1106 20-21 0.1919899072225988 1106 22-23 0.19152694702228246 1106 24-25 0.240437375101898 1106 26-27 0.2290156756705244 1106 28-29 0.28798584668253113 1106 30-31 0.1933532135783267 1106 32-33 0.18429661612101 1106 34-35 0.15146043892478644 1106 36-37 0.2385314928052722 1106 38-39 0.362841492779161 1106 40-41 0.24522552204021508 1106 42-43 0.19960557352102626 1106 44-45 0.09596459738164498 1106 46-47 0.12141816149275542 1106 48-49 0.17873460116237538 1106 50-51 0.18861946171508492 1106 52-53 0.2797819880396091 1106 54-55 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2216 86-87 -0.3382880064672378 2216 88-89 -0.2093651714407585 2216 90-91 -0.08913203599090735 2216 92-93 -0.2767142240105187 2216 94-95 -0.13502687455188322 2216 96-97 -0.1887635789189872 2216 98-99 -0.2937453778819581 2216 100-101 -0.2671677748144532 2216 102-103 -0.27602639258049066 2216 104-105 -0.39721085695846625 2216 106-107 -0.4041219394354023 2216 108-109 -0.2774480380543416 2216 110-111 -0.4050532530915021 2216 112-113 -0.3815111798155044 2216 114-115 -0.5279536932221163 2216 116-117 -0.7335240005585639 2216 118-119 -0.5926706530392778 2216 120-121 -0.682089831651453 2216 122-123 -0.7685879250248178 2216 124-125 -0.9531334856521809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48.0 3 3.0 4 12.0 5 42.0 6 108.0 7 208.0 8 408.0 9 892.0 10 1827.0 11 2495.0 12 2895.0 13 4549.0 14 8123.0 15 8344.0 16 2724.0 17 1744.0 18 1529.0 19 1501.0 20 1499.0 21 1619.0 22 1700.0 23 1322.0 24 1549.0 25 1904.0 26 2395.0 27 2962.0 28 3698.0 29 4688.0 30 5846.0 31 7546.0 32 9742.0 33 12823.0 34 18754.0 35 32395.0 36 66381.0 37 194038.0 38 154837.0 39 809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02311146422263 20.070902113230566 15.37643027899751 21.529556143549293 2 14.42232502717396 21.885456212242378 41.465248360253135 22.22697040033052 3 18.33909911890758 27.781274879911482 31.012006191939484 22.86761980924145 4 11.514844164203426 18.43857443537562 39.58762959718703 30.458951803233923 5 11.630892143808255 39.046604527296935 35.049090102086105 14.273413226808698 6 27.748116441088804 38.675151917071986 18.632560168381033 14.944171473458177 7 25.014052439982336 34.36402291655954 23.086961995464208 17.534962647993915 8 24.12959098090464 35.03268854650781 21.397477476199512 19.440242996388037 9 25.905074659682708 16.323349747056078 22.545255949457317 35.22631964380389 10-11 23.542491564103063 26.670644497206357 28.967176691922642 20.81968724676794 12-13 27.05905925785385 25.088791915724368 28.68222689947319 19.16992192694859 14-15 21.8584152394057 27.3331040022413 28.757764305561217 22.05071645279178 16-17 19.23091926895395 29.557999783672216 30.57943928547997 20.63164166189386 18-19 19.65001001845879 26.83404290028176 33.7810018104153 19.734945270844158 20-21 21.370041068781344 25.70646815573509 32.76414532248569 20.15934545299788 22-23 20.173878504921475 26.991774195550374 33.052785939353534 19.781561360174624 24-25 20.774293875973253 26.225665340920173 33.664503979899244 19.335536803207326 26-27 19.886640695511552 26.58544326803899 33.929416854771354 19.5984991816781 28-29 19.42800806441603 27.135217276433217 33.04149769752765 20.395276961623097 30-31 20.21175298204302 26.035580600717427 33.19221787399439 20.560448543245165 32-33 19.64557707209212 26.71435334838171 33.62966102145724 20.01040855806894 34-35 20.315217950241063 26.51132440478829 33.498179832221844 19.6752778127488 36-37 19.855432753090206 25.918018863073378 33.484526357412506 20.742022026423907 38-39 20.36380304241975 26.556717775582978 32.84263572351891 20.236843458478365 40-41 21.01021528160735 25.906315884665375 32.98901161254631 20.094457221180974 42-43 20.049773121804954 26.906743220695123 32.282754597408676 20.760729060091247 44-45 20.253954631453706 26.476392787418945 31.868894724616503 21.400757856510847 46-47 21.52239790481223 26.294287350676203 31.008725811628153 21.174588932883417 48-49 21.076177523543375 26.585620585893654 30.446096258770584 21.892105631792383 50-51 20.210777733842352 27.554574002720056 30.70781741225869 21.526830851178897 52-53 20.77553510095592 27.68330676520811 29.85740098127701 21.683757152558965 54-55 20.42276122909644 26.742990181910386 30.287485437771185 22.546763151221985 56-57 21.44136364522953 26.795564925819075 28.9540551706773 22.809016258274095 58-59 21.772770715601666 26.91277202775379 28.994926936177983 22.31953032046656 60-61 21.35376862502416 26.659473472362354 30.50602969364794 21.480728208965548 62-63 21.3719437051275 26.81586782017842 30.150596053968464 21.66159242072562 64-65 21.285412592050132 27.563971849017392 29.467922313501514 21.68269324543096 66-67 21.65902131183295 27.369010867811312 28.800231931753906 22.17173588860183 68-69 21.883771692623043 26.63642215125568 29.886569768369686 21.593236387751592 70-71 22.497734764406633 26.686869080908366 29.28209320181077 21.533302952874234 72-73 21.733494810792983 26.823492487929084 29.711645704740945 21.731366996536984 74-75 20.66107642576854 28.10266349149495 29.677600676644932 21.558659406091575 76-77 20.915970841851976 26.723219241115046 30.092967751201773 22.267842165831205 78-79 20.853732274863955 26.679599048867026 29.93480022483904 22.53186845142998 80-81 21.124319321085398 27.107467032177873 29.559772962218883 22.208440684517846 82-83 20.602472874801183 26.729159389246384 30.46258681925459 22.205780916697844 84-85 20.710104812583893 26.649632331428347 29.915915873317033 22.724346982670728 86-87 19.985584058415597 27.652719435278094 29.024095723270666 23.337600783035647 88-89 20.700706966286557 26.586063880530318 29.291225071326103 23.422004081857015 90-91 20.758360396734837 26.42283450674568 29.750591139163067 23.068213957356413 92-93 20.654338347291205 27.27317056736394 29.73558361512096 22.3369074702239 94-95 20.332100671063586 26.966223398832 29.692856971871006 23.008818958233405 96-97 20.321424075154397 26.65388795994035 29.975228695703056 23.049459269202195 98-99 20.739393756699414 26.557123567134234 29.61041655399385 23.093066122172505 100-101 20.3709318779058 26.319335837065882 29.894868057550383 23.41486422747793 102-103 20.3732912024243 26.523676914797505 29.63570771859177 23.467324164186422 104-105 21.023242617820078 26.2206696604707 29.34244182695931 23.41364589474991 106-107 20.5488889401175 26.01038088668448 29.854891021969387 23.58583915122863 108-109 20.065288723509703 26.735484031126518 29.164749408863983 24.0344778364998 110-111 19.86604827333671 27.31913014720681 29.184772345015247 23.630049234441234 112-113 20.223722850373818 26.05645916384117 28.83543886280129 24.884379122983727 114-115 19.687052802746443 26.523995041929037 28.705741325592406 25.08321082973211 116-117 20.194910377931436 26.329037160110403 28.349512325084692 25.12654013687347 118-119 19.923859443184497 25.890537753764914 29.42644563326637 24.75915716978422 120-121 19.475721510188738 26.571221868372707 29.176300988020344 24.776755633418208 122-123 19.86617690997573 26.494737598046648 28.46050250596902 25.178582986008596 124-125 20.00858235156433 26.557945058764282 28.146478044074986 25.286994545596404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 788.0 1 528.5 2 363.0 3 392.0 4 217.5 5 149.0 6 348.0 7 930.5 8 1659.0 9 1833.5 10 1180.0 11 540.0 12 425.0 13 452.0 14 485.0 15 554.5 16 633.5 17 740.5 18 858.0 19 930.0 20 1008.5 21 1096.0 22 1306.0 23 1451.0 24 1582.0 25 1969.0 26 2612.0 27 3392.5 28 4170.0 29 5240.0 30 6613.5 31 8194.5 32 10591.5 33 13412.5 34 16865.5 35 20340.0 36 22894.5 37 23549.0 38 22362.5 39 20771.5 40 19700.5 41 19294.5 42 18844.0 43 19027.5 44 19627.5 45 20285.5 46 20324.0 47 22668.0 48 28475.0 49 26943.0 50 20765.5 51 17341.5 52 14649.5 53 13153.5 54 12085.5 55 11261.5 56 10196.5 57 8642.0 58 7364.0 59 6526.0 60 5598.5 61 4868.0 62 4053.5 63 2746.5 64 1721.5 65 1328.5 66 995.0 67 752.0 68 497.0 69 261.5 70 180.5 71 188.5 72 152.5 73 113.0 74 104.5 75 70.5 76 50.5 77 27.5 78 16.5 79 6.0 80 3.5 81 5.0 82 2.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014717381937339418 2 0.0 3 0.0 4 0.0 5 0.12571835895871863 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0047875820760019784 22-23 3.5463570933347993E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.752482006670697E-4 92-93 0.0 94-95 6.206124913335898E-4 96-97 0.0 98-99 0.0018618374740007695 100-101 1.7731785466673996E-4 102-103 4.432946366668499E-4 104-105 0.0029257446020012094 106-107 0.006738078477336118 108-109 4.432946366668499E-4 110-111 0.0043442874393351295 112-113 0.00833393916933678 114-115 0.005319535640002199 116-117 0.0013298839100005498 118-119 3.5463570933347993E-4 120-121 5.3195356400022E-4 122-123 9.752482006670697E-4 124-125 0.0019504964013341394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 563959.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.39385976768277 #Duplication Level Percentage of deduplicated Percentage of total 1 68.6772222407109 31.862014178695265 2 14.769528199876206 13.704308402797857 3 6.271188040680944 8.72833855608363 4 3.298882921028072 6.121916465127204 5 1.911752690853885 4.434679312498267 6 1.2589348574694108 3.504410834045012 7 0.8428242945874137 2.737131049231923 8 0.5861832047718875 2.1756241120286255 9 0.4296344867337848 1.7939161915998818 >10 1.84649073566786 14.091287074604175 >50 0.058836948622718585 1.8668128216523503 >100 0.03973404264313472 3.6942757677870848 >500 0.005730871535067506 1.9144934539808225 >1k 0.00305646481870267 3.370791779867911 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4362 0.7734604820563197 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3460 0.6135197771469203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3450 0.6117465986002528 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2017 0.35765011286281445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2015 0.35729547715348103 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1647 0.29204250663612075 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1053 0.1867157009640772 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1013 0.17962298677740757 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 996 0.176608583248073 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 917 0.16260047272940056 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 892 0.15816752636273204 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 859 0.15231603715872963 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 848 0.15036554075739547 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.14770577293739437 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 793 0.1406130587507248 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 734 0.13015130532538713 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 712 0.12625031252271884 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 618 0.10958243418404529 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.7731785466673996E-4 0.0 10-11 0.0 0.0 0.0 1.7731785466673996E-4 0.0 12-13 0.0 0.0 0.0 1.7731785466673996E-4 0.0 14-15 0.0 0.0 0.0 1.7731785466673996E-4 0.0 16-17 0.0 0.0 0.0 1.7731785466673996E-4 0.0 18-19 0.0 0.0 0.0 1.7731785466673996E-4 0.0 20-21 0.0 0.0 0.0 1.7731785466673996E-4 0.0 22-23 0.0 0.0 0.0 9.752482006670697E-4 0.0 24-25 0.0 0.0 0.0 0.0026597678200010995 0.0 26-27 0.0 0.0 0.0 0.0063834427680026385 0.0 28-29 0.0 0.0 0.0 0.015337994428673006 0.0 30-31 0.0 0.0 0.0 0.034842958442014405 0.0 32-33 0.0 0.0 0.0 0.06835603297402826 0.0 34-35 0.0 0.0 0.0 0.11782771442604871 0.0 36-37 0.0 0.0 0.0 0.17971164570474096 0.0 38-39 0.0 0.0 0.0 0.2778570782627815 0.0 40-41 0.0 0.0 0.0 0.39382295521482946 0.0 42-43 0.0 0.0 0.0 0.49241168240953687 0.0 44-45 0.0 0.0 0.0 0.6035013183582494 0.0 46-47 0.0 0.0 0.0 0.7348051897389705 0.0 48-49 0.0 0.0 0.0 0.8867665911883665 0.0 50-51 0.0 0.0 0.0 1.0372207908730955 0.0 52-53 0.0 0.0 0.0 1.1831533852638225 0.0 54-55 0.0 0.0 0.0 1.3399910277165539 0.0 56-57 0.0 0.0 0.0 1.5227170769506295 0.0 58-59 0.0 0.0 0.0 1.7241501598520461 0.0 60-61 0.0 0.0 0.0 1.9501417656248061 0.0 62-63 0.0 0.0 0.0 2.1704592000482306 0.0 64-65 0.0 0.0 0.0 2.3865210059596533 0.0 66-67 0.0 0.0 0.0 2.6387556542230906 0.0 68-69 0.0 0.0 0.0 2.9465794499245512 0.0 70-71 0.0 0.0 0.0 3.279848357770689 0.0 72-73 0.0 0.0 0.0 3.5977792711881538 0.0 74-75 0.0 0.0 0.0 3.9200544720449537 0.0 76-77 0.0 0.0 0.0 4.267154172555097 0.0 78-79 0.0 0.0 0.0 4.687486147042604 0.0 80-81 0.0 0.0 0.0 5.113758269661448 0.0 82-83 0.0 0.0 0.0 5.548541649304294 0.0 84-85 0.0 0.0 0.0 5.9857188199851405 0.0 86-87 0.0 0.0 0.0 6.454458568796667 0.0 88-89 0.0 0.0 0.0 6.9568887099948755 0.0 90-91 0.0 0.0 0.0 7.5129575022297725 0.0 92-93 0.0 0.0 0.0 8.109986718892685 0.0 94-95 0.0 0.0 0.0 8.685560475140925 0.0 96-97 0.0 0.0 0.0 9.26140020817116 0.0 98-99 0.0 0.0 0.0 9.882633311996084 0.0 100-101 0.0 0.0 0.0 10.57940382190904 0.0 102-103 0.0 1.7731785466673996E-4 0.0 11.330876889986683 0.0 104-105 0.0 1.7731785466673996E-4 0.0 12.051762628134316 0.0 106-107 0.0 1.7731785466673996E-4 0.0 12.786567817873285 0.0 108-109 0.0 1.7731785466673996E-4 0.0 13.546286166192932 0.0 110-111 0.0 1.7731785466673996E-4 0.0 14.356806079874602 0.0 112-113 0.0 1.7731785466673996E-4 0.0 15.231515056945629 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGAGC 25 5.3348136E-4 47.59592 52-53 GTAATCG 35 0.002790136 33.997086 30-31 AACGTGC 40 0.0053644073 29.747454 16-17 GTTGCGG 80 0.0053621605 29.747454 8 GTACATG 3265 0.0 29.006449 1 GAGTACT 3995 0.0 28.072062 12-13 TACATGG 3310 0.0 27.70743 2 AGTACTT 3975 0.0 27.689451 12-13 ACATGGG 3310 0.0 26.62792 3 CTGTCGA 45 0.009532869 26.44218 50-51 GTACTTT 4340 0.0 25.429272 14-15 GTATAAG 265 1.3569661E-9 24.724495 1 TAGGACC 715 0.0 24.154356 4 TAGGACG 1310 0.0 24.093956 4 AGAGTAC 6385 0.0 23.947048 10-11 TGTAGGA 1920 0.0 23.883226 2 GTAGGAC 1525 0.0 23.821192 3 AGGACGT 1330 0.0 23.73164 5 TACTTTT 4685 0.0 23.239204 14-15 CATGGGA 2220 0.0 23.070074 4 >>END_MODULE