FastQCFastQC Report
Fri 27 May 2016
SRR939936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472429
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8540.18076790374849977No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8260.17484108723215552No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA6170.13060163537801447No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA5620.11895967436376684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG52.3646269E-45864.218110-111
CGTCGTC52.3646269E-45864.218110-111
CCGCGCA53.3026052E-44964.798108-109
CGCCGTT109.457448E-42932.109110-111
CTATGCG100.00132086692482.399108-109
CCGCTCG401.8189894E-122482.399108-109
CGCGTCG100.00132086692482.399108-109
GCGCTCG100.00132086692482.399108-109
CCGCTCC750.01985.9191108-109
TTCGTCG150.00212768741954.739110-111
CGTCGCT150.00212768741954.739110-111
CGCGTTC150.00212768741954.739110-111
CAGCGCG304.727126E-61654.9325108-109
CGCGGCG150.00297155651654.9325108-109
GCGCGAT150.00297155651654.9325108-109
CCGCTAC150.00297155651654.9325108-109
CCTCGCG150.00297155651654.9325108-109
CCATGCG150.00297155651654.9325108-109
CATCGCG150.00297155651654.9325108-109
CCGCACG150.00297155651654.9325108-109
Produced by FastQC (version 0.11.5)