##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939936_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 472429 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.336715146614623 33.0 31.0 34.0 28.0 34.0 2 31.477161647570323 34.0 31.0 34.0 28.0 34.0 3 31.634016963395556 34.0 31.0 34.0 28.0 34.0 4 34.797997159361515 37.0 35.0 37.0 32.0 37.0 5 34.87400858118363 37.0 35.0 37.0 32.0 37.0 6 34.910232013699414 37.0 35.0 37.0 32.0 37.0 7 34.83013320520121 37.0 35.0 37.0 32.0 37.0 8 34.810540843174316 37.0 35.0 37.0 32.0 37.0 9 36.34478408395759 39.0 38.0 39.0 32.0 39.0 10-11 36.333688236750916 39.0 38.0 39.0 32.0 39.0 12-13 36.25498752193451 39.0 38.0 39.0 32.0 39.0 14-15 37.708084177728296 41.0 39.0 41.0 32.0 41.0 16-17 37.729162477324635 41.0 39.0 41.0 32.0 41.0 18-19 37.764184671135766 41.0 39.0 41.0 32.0 41.0 20-21 37.82849168869819 41.0 39.0 41.0 32.0 41.0 22-23 37.85365419989036 41.0 39.0 41.0 32.0 41.0 24-25 37.915284624779595 41.0 39.0 41.0 32.0 41.0 26-27 37.901282520759736 41.0 38.0 41.0 32.0 41.0 28-29 37.861690328070466 40.0 38.0 41.0 32.0 41.0 30-31 37.77723636779284 40.0 38.0 41.0 32.0 41.0 32-33 37.67971589381685 40.0 38.0 41.0 31.0 41.0 34-35 37.618230464260236 40.0 38.0 41.0 31.0 41.0 36-37 37.78958425498858 40.5 38.0 41.0 31.0 41.0 38-39 37.73153743737154 41.0 38.0 41.0 31.0 41.0 40-41 37.64224782983263 40.5 38.0 41.0 31.0 41.0 42-43 37.58439151703219 40.0 38.0 41.0 30.5 41.0 44-45 37.51032641941964 40.0 38.0 41.0 30.0 41.0 46-47 37.411552847094484 40.0 38.0 41.0 30.0 41.0 48-49 37.319884046068296 40.0 38.0 41.0 30.0 41.0 50-51 37.210119404185605 40.0 38.0 41.0 30.0 41.0 52-53 37.12039586900889 40.0 37.5 41.0 30.0 41.0 54-55 36.98658528583131 40.0 37.0 41.0 30.0 41.0 56-57 36.788356557281624 40.0 37.0 41.0 30.0 41.0 58-59 36.62133463441067 40.0 36.5 41.0 30.0 41.0 60-61 36.419160339437255 40.0 36.0 41.0 30.0 41.0 62-63 36.16954399497067 40.0 36.0 41.0 29.0 41.0 64-65 35.888205423460455 39.0 35.0 41.0 29.0 41.0 66-67 35.59123910682875 39.0 35.0 41.0 28.5 41.0 68-69 35.18139339456299 38.5 35.0 40.5 28.0 41.0 70-71 34.75412390009927 37.5 34.0 40.0 27.5 41.0 72-73 34.35408495244788 37.0 34.0 39.5 26.5 41.0 74-75 33.91298163321896 37.0 34.0 39.0 26.0 41.0 76-77 33.41337534317326 36.0 34.0 39.0 26.0 40.5 78-79 32.92078598053888 36.0 33.5 38.5 24.5 40.0 80-81 32.44854041559684 35.0 33.0 37.0 22.0 39.0 82-83 32.18824415943983 35.0 33.0 37.0 21.0 39.0 84-85 31.67455956344763 35.0 33.0 36.5 7.5 39.0 86-87 30.911068118172253 35.0 33.0 36.0 2.0 37.0 88-89 15.590432636438491 18.0 16.0 18.5 2.0 19.5 90-91 2.7773908883662943 2.0 2.0 2.0 2.0 2.0 92-93 2.2970869696822165 2.0 2.0 2.0 2.0 2.0 94-95 2.0969288506844412 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.287516049256336 1101 2 0.25975586474227086 1101 3 0.3153851447128453 1101 4 0.3324775522079122 1101 5 0.2235054904766045 1101 6 0.28161641848103613 1101 7 0.39489259628431483 1101 8 0.28291727342755735 1101 9 0.49336026849634607 1101 10-11 0.4229495257002682 1101 12-13 0.4839845341152724 1101 14-15 0.38530048895508173 1101 16-17 0.3529487885557856 1101 18-19 0.32307279464680505 1101 20-21 0.4750621288107837 1101 22-23 0.4013209069437593 1101 24-25 0.39589298115490124 1101 26-27 0.4335536199684782 1101 28-29 0.4960965169494713 1101 30-31 0.3874249865563755 1101 32-33 0.547911663663875 1101 34-35 0.496655092373274 1101 36-37 0.5035301193997128 1101 38-39 0.48218770168664804 1101 40-41 0.4895438744672944 1101 42-43 0.3687943085797656 1101 44-45 0.272302826605312 1101 46-47 0.263419890692731 1101 48-49 0.4064466381989149 1101 50-51 0.4665966839022673 1101 52-53 0.6399391233136527 1101 54-55 0.48115369777779904 1101 56-57 0.3005542693167058 1101 58-59 0.37418943408620464 1101 60-61 0.3222669887592815 1101 62-63 0.5434469773992774 1101 64-65 0.47206941283715054 1101 66-67 0.4095845368027895 1101 68-69 0.22965892101655783 1101 70-71 0.1773273526033421 1101 72-73 0.2560535435452067 1101 74-75 0.30919808060743037 1101 76-77 0.2648678706504839 1101 78-79 0.3502894287946958 1101 80-81 0.478994520327241 1101 82-83 0.47915220987737683 1101 84-85 0.446678283525312 1101 86-87 0.8447659582395595 1101 88-89 0.5764799255037332 1101 90-91 -0.2283037131868646 1101 92-93 -0.22327172046215837 1101 94-95 -0.043017274873969225 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.19062903875228088 1102 2 -0.03611631241924229 1102 3 0.18171469743387902 1102 4 0.25478428663392094 1102 5 0.1369903287628773 1102 6 -0.07185470526951576 1102 7 0.08069090005707835 1102 8 0.053928699402725044 1102 9 -0.05212066577485075 1102 10-11 0.06976867893286709 1102 12-13 -0.1505188818979164 1102 14-15 -0.020937942034720436 1102 16-17 0.02806153381879284 1102 18-19 -0.06287990282568501 1102 20-21 -0.06828274548666258 1102 22-23 -0.13208618759398405 1102 24-25 0.029197427020349664 1102 26-27 0.027740087171387984 1102 28-29 0.14773994743444518 1102 30-31 0.14214409098877923 1102 32-33 0.10592288770723002 1102 34-35 0.15886313262501517 1102 36-37 0.09096764674464453 1102 38-39 0.2559851556630264 1102 40-41 0.2920072322568146 1102 42-43 0.25335872482854427 1102 44-45 0.09208394093237615 1102 46-47 0.1365572800509227 1102 48-49 0.09448512303719525 1102 50-51 0.2445968437649526 1102 52-53 0.2844896988193284 1102 54-55 0.3402726019611464 1102 56-57 0.11150822886303047 1102 58-59 0.06553139719999024 1102 60-61 0.14044947622268467 1102 62-63 0.007556752581855619 1102 64-65 0.18028845837277885 1102 66-67 0.12496585137890293 1102 68-69 0.06170213442482719 1102 70-71 0.14166009416427983 1102 72-73 -0.032392380797674036 1102 74-75 0.1142739312451937 1102 76-77 -0.006585281168582924 1102 78-79 -0.08215386202076758 1102 80-81 0.08866978872827502 1102 82-83 0.08480356618606066 1102 84-85 -0.06129486795720496 1102 86-87 -0.035433785980142574 1102 88-89 0.19508015940808576 1102 90-91 0.06265584155348503 1102 92-93 0.011146926258132517 1102 94-95 0.1418470942573875 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.11736099447031023 1103 2 0.14501325568707202 1103 3 0.21380772068969378 1103 4 -0.02558116187105952 1103 5 0.055744481586799566 1103 6 -0.039005868060208115 1103 7 0.14562445487435127 1103 8 -0.06189355973349109 1103 9 0.034606908975980843 1103 10-11 -0.006385806116973924 1103 12-13 0.1998631779027491 1103 14-15 0.17098066261644362 1103 16-17 0.20090206538025512 1103 18-19 0.19322474833711567 1103 20-21 0.31501044388210886 1103 22-23 0.22982411140933579 1103 24-25 0.2185495864887841 1103 26-27 0.2679809509587585 1103 28-29 0.31825074477663406 1103 30-31 0.3735103700585469 1103 32-33 0.33257571162749144 1103 34-35 0.31980167083099076 1103 36-37 0.2985714673426543 1103 38-39 0.46916206596203125 1103 40-41 0.4155662023564872 1103 42-43 0.372735801240502 1103 44-45 0.3129767980752334 1103 46-47 0.45676907407086276 1103 48-49 0.3005731629043069 1103 50-51 0.2383219268214276 1103 52-53 0.2794357121083664 1103 54-55 0.3115516717285942 1103 56-57 0.4958271657401099 1103 58-59 0.30857541713355374 1103 60-61 0.14622605429577362 1103 62-63 0.17427186886093438 1103 64-65 0.10026354142925697 1103 66-67 0.13308877496694294 1103 68-69 0.16489150319559087 1103 70-71 0.1682090974865389 1103 72-73 0.14727124046478224 1103 74-75 0.20037442958406615 1103 76-77 0.26598116401414273 1103 78-79 0.2103793605705917 1103 80-81 0.3377520146419002 1103 82-83 0.3204887821329052 1103 84-85 0.5201876901823361 1103 86-87 0.337240632624507 1103 88-89 0.7660643786771892 1103 90-91 -0.6119330288784086 1103 92-93 -0.3166147016488443 1103 94-95 -0.10524592899842622 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.41413307020427226 1104 2 -0.1896613247468224 1104 3 -0.21051892687477647 1104 4 -0.27536830882153396 1104 5 -0.15917979184749953 1104 6 -0.10505171333241492 1104 7 -0.2797992045607671 1104 8 -0.24296191830901392 1104 9 -0.31798826089712406 1104 10-11 -0.0668217455357123 1104 12-13 0.029480451746380254 1104 14-15 -0.03545200214000488 1104 16-17 0.0325844231371164 1104 18-19 0.01492961939727877 1104 20-21 -0.14850572476299817 1104 22-23 -0.2439090036131759 1104 24-25 -0.07306318469419182 1104 26-27 0.04955074219904532 1104 28-29 -0.019813111138219597 1104 30-31 -0.08928332621652402 1104 32-33 -0.06186964244536597 1104 34-35 -0.11924458377400526 1104 36-37 -0.1436041364232068 1104 38-39 -0.17545725454554173 1104 40-41 -0.03608340877737248 1104 42-43 -0.21170341284054217 1104 44-45 -0.2826474344257903 1104 46-47 -0.1508731690728098 1104 48-49 -0.1208185685733838 1104 50-51 -0.13122985377619045 1104 52-53 -0.10106162722851764 1104 54-55 2.479468002292151E-4 1104 56-57 0.011130072002899283 1104 58-59 0.006231986924788657 1104 60-61 0.13298004529045215 1104 62-63 0.0758478000930225 1104 64-65 -0.08011918472710988 1104 66-67 -0.03586742642479379 1104 68-69 0.1481784454796653 1104 70-71 0.2849078285548927 1104 72-73 0.18186804766904174 1104 74-75 0.018249992416649263 1104 76-77 0.11912688648651226 1104 78-79 0.1722315914342829 1104 80-81 0.24274996797796433 1104 82-83 0.12226733309483251 1104 84-85 0.053840985311271794 1104 86-87 0.10672487331218505 1104 88-89 0.6188969615670814 1104 90-91 -0.652258448444516 1104 92-93 -0.3166147016488443 1104 94-95 -0.10524592899842622 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.2292377451705896 1105 2 0.24756227529491426 1105 3 0.39344357503143 1105 4 0.5282843843474296 1105 5 0.5180974227632689 1105 6 0.334523543704492 1105 7 0.42800540725530567 1105 8 0.21219607612085412 1105 9 0.25278618068466585 1105 10-11 0.2734321210538937 1105 12-13 0.5058715812640955 1105 14-15 0.38965013040355956 1105 16-17 0.5081849785455219 1105 18-19 0.44133399203458623 1105 20-21 0.5051224887000316 1105 22-23 0.3483955399807641 1105 24-25 0.2792218553963437 1105 26-27 0.37197254759742293 1105 28-29 0.21430116494470752 1105 30-31 0.22674566417619246 1105 32-33 0.27691744832217324 1105 34-35 0.5007260405788898 1105 36-37 0.3048319715443313 1105 38-39 0.2963469399116079 1105 40-41 0.5160704040371513 1105 42-43 0.5037862214085678 1105 44-45 0.3665062098399332 1105 46-47 0.5022032477403258 1105 48-49 0.20452274273624482 1105 50-51 0.20278971393627643 1105 52-53 0.1570407541251697 1105 54-55 0.10641161570617896 1105 56-57 0.1887263254039766 1105 58-59 0.17447177567696315 1105 60-61 0.11011961171874418 1105 62-63 0.0851962386088232 1105 64-65 0.041229927983884807 1105 66-67 0.24446132398654896 1105 68-69 0.36189430431604563 1105 70-71 0.20925951765460127 1105 72-73 0.1920331452266879 1105 74-75 0.18547246879975177 1105 76-77 0.25481869902814935 1105 78-79 0.324370957209247 1105 80-81 0.2546707821488994 1105 82-83 0.375124636474645 1105 84-85 0.4642633204344371 1105 86-87 0.7018064589550406 1105 88-89 0.5133472918424573 1105 90-91 -0.7883616003069802 1105 92-93 -0.3166147016488443 1105 94-95 -0.10524592899842622 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.5160351185330967 1106 2 -0.38665775212699316 1106 3 -0.4942287449484546 1106 4 -0.43612213562381186 1106 5 -0.5107191529553745 1106 6 -0.3045738905006061 1106 7 -0.42008782723344495 1106 8 -0.2701584365116929 1106 9 -0.11655117557621253 1106 10-11 -0.4605262589320418 1106 12-13 -0.23838867543325648 1106 14-15 -0.18654388156308244 1106 16-17 -0.4170402307527823 1106 18-19 -0.3268664157582606 1106 20-21 -0.31896871861037823 1106 22-23 -0.4232935002917202 1106 24-25 -0.40901402801732445 1106 26-27 -0.4998761918983803 1106 28-29 -0.3275476924102918 1106 30-31 -0.38393002264832177 1106 32-33 -0.3597825332132203 1106 34-35 -0.33910035281156325 1106 36-37 -0.2047234635329218 1106 38-39 -0.20280447380955735 1106 40-41 -0.1806690210456452 1106 42-43 -0.6446545013040748 1106 44-45 -0.10904684448136237 1106 46-47 -0.3358356143683281 1106 48-49 -0.12284298616401657 1106 50-51 -0.1871248774262142 1106 52-53 -0.3067263796568156 1106 54-55 -0.26504720625177214 1106 56-57 -0.18990815784233916 1106 58-59 -0.2717441581018747 1106 60-61 -0.18358159948102326 1106 62-63 -0.2937505715317812 1106 64-65 -0.35920850826018125 1106 66-67 -0.1554045129513142 1106 68-69 -0.18843648718709716 1106 70-71 -0.23811929361745143 1106 72-73 -0.38213368840718687 1106 74-75 -0.37669629772508983 1106 76-77 -0.3430260489692287 1106 78-79 -0.2628654801426933 1106 80-81 -0.2842335593913603 1106 82-83 -0.16747655547479212 1106 84-85 -0.32298902791023565 1106 86-87 -0.3091829297878341 1106 88-89 0.4146598505265189 1106 90-91 -0.11585108137289879 1106 92-93 -0.27524022759554834 1106 94-95 -0.10524592899842622 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.23862187824280667 1107 2 -0.13530204209416752 1107 3 -0.19755467713046926 1107 4 0.06566445899309059 1107 5 -0.0800982587791026 1107 6 -0.04758897432724041 1107 7 -0.06544210799057737 1107 8 -0.09246965938316265 1107 9 0.08895879687010222 1107 10-11 0.005375579636243799 1107 12-13 -0.10087057063753946 1107 14-15 0.10153924572271222 1107 16-17 -0.03308002881981764 1107 18-19 0.04015935324174791 1107 20-21 -0.17261509134560526 1107 22-23 -0.10080648415313931 1107 24-25 -0.03720362487750606 1107 26-27 -0.24238300388748257 1107 28-29 -0.15454800960249315 1107 30-31 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-0.18175371331327028 2216 38-39 -0.2697845710585369 2216 40-41 -0.15683883748046412 2216 42-43 -0.37782284245043485 2216 44-45 -0.3384851252154846 2216 46-47 -0.2530011686565814 2216 48-49 -0.3026489975552096 2216 50-51 -0.4204023644515047 2216 52-53 -0.3585752198908949 2216 54-55 -0.39883094824546816 2216 56-57 -0.4324357581064717 2216 58-59 -0.2747347551844044 2216 60-61 -0.29674040855395134 2216 62-63 -0.3392171064957523 2216 64-65 -0.4189276759137144 2216 66-67 -0.3956846176585884 2216 68-69 -0.32034473916127837 2216 70-71 -0.468532610869957 2216 72-73 -0.43155403283520144 2216 74-75 -0.5134888277811314 2216 76-77 -0.3320705313721817 2216 78-79 -0.4442722977581042 2216 80-81 -0.5039396659213793 2216 82-83 -0.3701995632441566 2216 84-85 -0.5276657469123514 2216 86-87 -0.7344843998009161 2216 88-89 -1.1387197606594786 2216 90-91 -0.7883616003069802 2216 92-93 -0.3166147016488443 2216 94-95 -0.10524592899842622 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7504.0 3 1200.0 4 847.0 5 739.0 6 729.0 7 753.0 8 848.0 9 1082.0 10 1225.0 11 1474.0 12 1835.0 13 2094.0 14 2368.0 15 2741.0 16 3282.0 17 3857.0 18 4987.0 19 5962.0 20 5522.0 21 7658.0 22 8506.0 23 10322.0 24 15349.0 25 25528.0 26 44414.0 27 120129.0 28 184393.0 29 6823.0 30 258.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5105761924014 20.475669359840314 15.1203672932864 22.89338715447189 2 12.89350244695443 20.75233265033106 45.69835571435829 20.655809188356223 3 18.51272256009758 28.14337020786393 29.31652238730116 24.02738484473733 4 10.985576057653414 18.335624024514985 40.34214243510708 30.336657482724515 5 11.492164337145278 39.452138924184666 34.40279542566709 14.652901313002964 6 27.305902050890186 37.22696955521359 22.15867357846364 13.308454815432583 7 27.190964144876794 32.534412578398026 23.06462981739055 17.20999345933463 8 24.539348769868063 38.55669317505911 21.34204293131878 15.561915123754044 9 25.99226550444617 19.159069405138126 22.529946298808923 32.318718791606784 10-11 23.19789427561748 27.589476881588986 30.44441833809069 18.768210504702846 12-13 25.663628772187547 25.50688315413249 29.592747154818316 19.236740918861653 14-15 20.381496882597048 30.38893716191974 26.45215096040988 22.77741499507333 16-17 19.535992786202343 31.094897000177806 30.155812102584946 19.21329811103491 18-19 20.660920848049805 28.90083964593677 31.336585510418573 19.10165399559485 20-21 22.08833811188478 28.98268185219406 28.86742509633835 20.06155493958281 22-23 20.37828066558431 31.215404708028966 29.028188482030025 19.378126144356695 24-25 20.734015058347392 29.482207908489954 30.81267238040002 18.971104652762637 26-27 19.156211832889174 30.950153356377356 29.954765689659187 19.93886912107428 28-29 19.10055754763709 31.207638868309534 28.275433612883287 21.416369971170088 30-31 20.179646042050763 29.452256317880572 29.93328097978744 20.43481666028123 32-33 19.179547968185258 30.47663902544862 30.803359281151938 19.54045372521419 34-35 19.518932494546796 30.99611898943438 30.3530587168217 19.131889799197126 36-37 19.072218412117824 30.451624374507862 29.899053400729848 20.577103812644467 38-39 20.272675894155523 31.068901358722684 28.953662878443108 19.70475986867868 40-41 21.929221110473744 28.76104134166192 30.331541882483933 18.978195665380408 42-43 19.449165906411334 30.99979044470174 30.309951336602957 19.24109231228396 44-45 19.146006530102504 31.12181234157622 29.420387255187304 20.311793873133972 46-47 20.69855999525855 29.42706734768611 29.451409629806808 20.42296302724854 48-49 19.802700093982576 30.012509842769692 29.53825768159381 20.646532381653923 50-51 18.79513915347947 31.40690221578738 29.577734596594613 20.220224034138536 52-53 18.83965632931086 30.54787068533049 30.242004618683442 20.370468366675205 54-55 19.149424825076757 29.817546407180878 30.271695932551758 20.761332835190608 56-57 19.92997889629976 30.27047450516374 28.220325170554727 21.57922142798177 58-59 20.006498330966135 30.91067652493814 27.948644134885875 21.134181009209847 60-61 19.55034513122607 28.971125819964485 31.05366097339494 20.42486807541451 62-63 19.959189634844602 29.009438455302277 30.41483482174041 20.61653708811271 64-65 20.436404200419535 30.03192014038088 28.72092949416738 20.810746165032207 66-67 20.240078403315632 31.43297723044098 27.99806955119182 20.328874815051574 68-69 21.148853527789978 29.056416063840484 30.1518222372745 19.642908171095037 70-71 21.792692658579384 29.00033655850932 29.373831835048232 19.833138947863066 72-73 20.572297636258572 30.46362522199103 28.81925114673316 20.14482599501724 74-75 19.99496220596111 30.668841243869448 29.995935897245936 19.340260652923508 76-77 20.240290075334073 29.392141464643363 30.56522779084265 19.80234066917992 78-79 19.857755951971466 29.320583581607764 29.964068560784458 20.857591905636312 80-81 20.212561040918317 30.171941180579516 29.626991569103506 19.98850620939866 82-83 19.797280420637307 30.10005799850135 30.66797763035904 19.4346839505023 84-85 19.89537052128468 29.83781689947061 30.41811573802624 19.84869684121847 86-87 18.5977003708533 31.6778995139959 28.67212504021811 21.052275074932687 88-89 29.182016944877525 18.886625099485485 35.93722841606039 15.994129539576601 90-91 31.245591680828355 15.697566679643227 18.011512267158146 35.04532937237027 92-93 22.54666147563861 18.00540080247605 37.2132712543266 22.234666467558743 94-95 17.329412192679644 7.5298154115378795 29.53645148258558 45.6043209131969 96-97 29.264340700273156 7.7710868305603835 16.06179124244682 46.90278122671964 98-99 57.14329857087679 14.244882193897258 18.702201622247973 9.909617612977984 100-101 31.255779463400962 7.578462767565706 12.83454679075639 48.33121097827694 102-103 24.749685900392368 2.428961233406112 37.76420150540615 35.057151360795366 104-105 42.488967716053295 5.273115010240493 41.160870758641074 11.077046515065138 106-107 7.37205547221236 2.402001763567445 7.865052735247301 82.36089002897289 108-109 18.912164778778198 11.793130990415337 23.85891664246297 45.4357875883435 110-111 41.369386622786934 2.957215526375376 26.595926720375555 29.077471130462136 112-113 29.02904660211866 3.0395488010026646 39.766578296492675 28.164826300385997 114-115 32.164851909463195 4.569696200120632 24.71667566109012 38.54877622932605 116-117 22.529572971980834 4.843701542414825 21.835581237855937 50.7911442477484 118-119 23.0908022987535 4.735324716206827 16.682854170425625 55.49101881461406 120-121 28.00417404915378 4.813200430448819 30.466210854701785 36.716414665695616 122-123 36.133858929606376 1.877192749602927 34.69510549531255 27.293842825478148 124-125 40.559758912541675 2.3067880653158928 20.754253227323247 36.379199794819186 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17.0 1 38.5 2 83.0 3 115.5 4 134.5 5 133.0 6 103.5 7 73.5 8 63.0 9 67.0 10 62.5 11 50.0 12 42.5 13 49.0 14 54.0 15 82.5 16 123.5 17 157.5 18 224.0 19 334.5 20 465.5 21 667.0 22 987.0 23 1366.0 24 1874.5 25 2600.5 26 3497.0 27 4641.0 28 6032.0 29 7946.5 30 10332.5 31 13104.5 32 16574.0 33 20387.0 34 23599.0 35 25944.5 36 26924.5 37 26574.5 38 25544.5 39 23918.5 40 22155.0 41 20499.0 42 18829.0 43 17300.5 44 16087.0 45 15041.5 46 14113.0 47 13097.0 48 12372.5 49 11606.5 50 10518.0 51 9547.0 52 8528.0 53 7431.5 54 6295.5 55 5263.5 56 4305.5 57 3423.0 58 2747.5 59 2128.5 60 1623.5 61 1249.0 62 932.0 63 679.0 64 479.0 65 336.0 66 236.0 67 172.0 68 125.5 69 88.0 70 56.0 71 41.0 72 36.0 73 24.5 74 18.0 75 13.0 76 10.5 77 9.0 78 6.0 79 3.0 80 1.0 81 1.0 82 1.5 83 2.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.5 89 2.0 90 1.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 2.0 97 2.5 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0010583600922043311 3 0.04169938763285065 4 0.0482612202045175 5 0.04847289222295837 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.291800461021656E-4 12-13 0.0015875401383064968 14-15 1.0583600922043313E-4 16-17 2.1167201844086625E-4 18-19 0.005820980507123821 20-21 0.0011641961014247644 22-23 4.233440368817325E-4 24-25 0.0 26-27 0.0 28-29 6.350160553225988E-4 30-31 0.0 32-33 0.0013758681198656306 34-35 1.0583600922043313E-4 36-37 2.1167201844086625E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.175080276612994E-4 46-47 0.0 48-49 2.1167201844086625E-4 50-51 2.1167201844086625E-4 52-53 0.0 54-55 0.0015875401383064968 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.175080276612994E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.175080276612994E-4 80-81 0.0 82-83 6.350160553225988E-4 84-85 0.0 86-87 0.0010583600922043311 88-89 19.01566161264444 90-91 26.32469640940755 92-93 40.466292289423386 94-95 44.72216989219544 96-97 48.8558069043179 98-99 93.14976430320746 100-101 52.044328354101886 102-103 53.41659804965402 104-105 74.93750383655534 106-107 81.51616433368824 108-109 12.545588860971701 110-111 38.76847102950919 112-113 52.37369001479587 114-115 86.66423949418855 116-117 66.55603275836157 118-119 72.43088379417858 120-121 80.52680931949563 122-123 76.07799267191471 124-125 50.481448005943754 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 472429.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.77541239296758 #Duplication Level Percentage of deduplicated Percentage of total 1 92.45870858707535 77.4576644120345 2 5.56527933721767 9.324671431149431 3 0.9512845789072681 2.3908277370308064 4 0.34346829602709256 1.150967925743182 5 0.17337448422690013 0.7262259457263306 6 0.1010506949117111 0.5079338183294729 7 0.06489687035746244 0.38057334540465587 8 0.04416470428696283 0.2959933051883019 9 0.03753529908407118 0.2830081644055297 >10 0.22484318121062385 3.5084173451024667 >50 0.019466682981499377 1.12908457620041 >100 0.014916027604603217 2.239107247207021 >500 0.0010112561087865504 0.6055247464779105 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 854 0.18076790374849977 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 826 0.17484108723215552 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 617 0.13060163537801447 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 562 0.11895967436376684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0583600922043313E-4 0.0 12-13 0.0 0.0 0.0 2.1167201844086625E-4 0.0 14-15 0.0 0.0 0.0 4.233440368817325E-4 0.0 16-17 0.0 0.0 0.0 4.233440368817325E-4 0.0 18-19 0.0 0.0 0.0 4.233440368817325E-4 0.0 20-21 0.0 0.0 0.0 5.291800461021657E-4 0.0 22-23 0.0 0.0 0.0 9.525240829838981E-4 0.0 24-25 0.0 0.0 0.0 0.0014817041290860638 0.0 26-27 0.0 0.0 0.0 0.00169337614752693 0.0 28-29 0.0 0.0 0.0 0.0021167201844086627 0.0 30-31 0.0 0.0 0.0 0.004127604359596892 0.0 32-33 0.0 0.0 0.0 0.008784388765295949 0.0 34-35 0.0 0.0 0.0 0.013441173170995007 0.0 36-37 0.0 0.0 0.0 0.023389758037715723 0.0 38-39 0.0 0.0 0.0 0.044662795891022775 0.0 40-41 0.0 0.0 0.0 0.07567274659260968 0.0 42-43 0.0 0.0 0.0 0.10795272940484178 0.0 44-45 0.0 0.0 0.0 0.1370576319404609 0.0 46-47 0.0 0.0 0.0 0.17071348287255864 0.0 48-49 0.0 0.0 0.0 0.21050782233944149 0.0 50-51 0.0 0.0 0.0 0.2610974347468085 0.0 52-53 0.0 0.0 0.0 0.3069244267392561 0.0 54-55 0.0 0.0 0.0 0.35984243134947264 0.0 56-57 0.0 0.0 0.0 0.42281485683563036 0.0 58-59 0.0 0.0 0.0 0.4901265586998258 0.0 60-61 0.0 0.0 0.0 0.5719377938272205 0.0 62-63 0.0 0.0 0.0 0.6577707973049918 0.0 64-65 0.0 0.0 0.0 0.7443446528473061 0.0 66-67 0.0 0.0 0.0 0.8407612572471207 0.0 68-69 0.0 0.0 0.0 0.9645893880350276 0.0 70-71 1.0583600922043313E-4 0.0 0.0 1.1025995440584722 0.0 72-73 2.1167201844086625E-4 0.0 0.0 1.2353178996208953 0.0 74-75 2.1167201844086625E-4 0.0 0.0 1.3722696955521358 0.0 76-77 2.1167201844086625E-4 0.0 0.0 1.5234035167189144 0.0 78-79 2.1167201844086625E-4 0.0 0.0 1.5642562162780016 0.0 80-81 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 0.0 82-83 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 0.0 84-85 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 0.0 86-87 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 0.0 88-89 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 90-91 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 92-93 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 94-95 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 96-97 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 98-99 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 100-101 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 102-103 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 104-105 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 106-107 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 108-109 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 110-111 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 112-113 2.1167201844086625E-4 0.0 0.0 1.5646795603148833 2.1167201844086625E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCG 5 2.3646269E-4 5864.218 110-111 CGTCGTC 5 2.3646269E-4 5864.218 110-111 CCGCGCA 5 3.3026052E-4 4964.798 108-109 CGCCGTT 10 9.457448E-4 2932.109 110-111 CTATGCG 10 0.0013208669 2482.399 108-109 CCGCTCG 40 1.8189894E-12 2482.399 108-109 CGCGTCG 10 0.0013208669 2482.399 108-109 GCGCTCG 10 0.0013208669 2482.399 108-109 CCGCTCC 75 0.0 1985.9191 108-109 TTCGTCG 15 0.0021276874 1954.739 110-111 CGTCGCT 15 0.0021276874 1954.739 110-111 CGCGTTC 15 0.0021276874 1954.739 110-111 CAGCGCG 30 4.727126E-6 1654.9325 108-109 CGCGGCG 15 0.0029715565 1654.9325 108-109 GCGCGAT 15 0.0029715565 1654.9325 108-109 CCGCTAC 15 0.0029715565 1654.9325 108-109 CCTCGCG 15 0.0029715565 1654.9325 108-109 CCATGCG 15 0.0029715565 1654.9325 108-109 CATCGCG 15 0.0029715565 1654.9325 108-109 CCGCACG 15 0.0029715565 1654.9325 108-109 >>END_MODULE