FastQCFastQC Report
Fri 27 May 2016
SRR939936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472429
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.33084336482307397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12140.2569698303872116No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8850.18732973632016664No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8060.1706076468633382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.15007546107457417No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5930.12552150693543368No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA5800.12276977069570243No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4970.10520099316511053No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG251.7155135E-595.301994
CGGACGT453.152303E-452.9455455
TACCTGG7200.047.9819032
ACCTGGG8200.039.9512863
ACGTAGA600.001300863239.709161
GTACCTG8900.038.816821
GTATCAA32550.038.0622371
GTACATG30550.033.3400961
TACATGG30850.032.8228072
ATCAACG38100.032.2050673
TCAACGC38850.031.5833474
TATCAAC39050.031.4215892
CATGGGG13650.031.4182364
CAACGCA39700.031.0571635
ACATGGG30850.030.8920543
AACGCAG40300.030.7067816
CATGGGC5650.030.5725384
AGCGGAA400.005355876429.756643104-105
TATGCGA400.005364198229.74719674-75
TAGGACC7550.029.1901784
Produced by FastQC (version 0.11.5)