##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 472429 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80310057172612 34.0 31.0 34.0 31.0 34.0 2 32.87395566317902 34.0 31.0 34.0 31.0 34.0 3 32.90734692408807 34.0 31.0 34.0 31.0 34.0 4 36.33615633248594 37.0 37.0 37.0 35.0 37.0 5 36.2523828977476 37.0 37.0 37.0 35.0 37.0 6 36.38731322590273 37.0 37.0 37.0 35.0 37.0 7 36.3803386328951 37.0 37.0 37.0 35.0 37.0 8 36.40746228533811 37.0 37.0 37.0 35.0 37.0 9 38.22801733170487 39.0 39.0 39.0 37.0 39.0 10-11 38.21063165047023 39.0 39.0 39.0 37.0 39.0 12-13 38.213241566457604 39.0 39.0 39.0 37.0 39.0 14-15 39.71845398991171 41.0 40.0 41.0 37.0 41.0 16-17 39.74099811823576 41.0 40.0 41.0 37.0 41.0 18-19 39.80439177103861 41.0 40.0 41.0 37.5 41.0 20-21 39.822279114956956 41.0 40.0 41.0 37.5 41.0 22-23 39.76894834991078 41.0 40.0 41.0 37.0 41.0 24-25 39.74313283054173 41.0 40.0 41.0 37.0 41.0 26-27 39.640619013650735 41.0 40.0 41.0 37.0 41.0 28-29 39.55361334719079 41.0 40.0 41.0 36.5 41.0 30-31 39.38819801493981 41.0 39.0 41.0 36.0 41.0 32-33 39.2003316900529 41.0 39.0 41.0 35.0 41.0 34-35 39.07659246151273 41.0 39.0 41.0 35.0 41.0 36-37 39.038484089672735 41.0 39.0 41.0 35.0 41.0 38-39 39.066712670052006 41.0 39.0 41.0 35.0 41.0 40-41 38.95650351693058 41.0 39.0 41.0 35.0 41.0 42-43 38.834372995730575 41.0 39.0 41.0 35.0 41.0 44-45 38.65020034756545 41.0 39.0 41.0 35.0 41.0 46-47 38.358901549227504 41.0 39.0 41.0 35.0 41.0 48-49 38.0604408281456 41.0 39.0 41.0 34.5 41.0 50-51 37.893365987270045 41.0 39.0 41.0 34.0 41.0 52-53 37.79047645254631 41.0 39.0 41.0 34.0 41.0 54-55 37.70094448054628 41.0 39.0 41.0 34.0 41.0 56-57 37.575482241776015 41.0 38.5 41.0 33.0 41.0 58-59 37.430767374568454 40.5 38.0 41.0 33.0 41.0 60-61 37.271255574911784 40.0 38.0 41.0 33.0 41.0 62-63 37.08207794187063 40.0 37.0 41.0 32.5 41.0 64-65 36.85777439572931 40.0 37.0 41.0 32.0 41.0 66-67 36.60059501004383 39.0 36.5 41.0 32.0 41.0 68-69 36.27137093616183 39.0 36.0 41.0 31.0 41.0 70-71 35.91934978589375 39.0 35.0 41.0 31.0 41.0 72-73 35.580409966365316 37.5 35.0 40.0 31.0 41.0 74-75 35.22446653359552 37.0 35.0 39.5 31.0 41.0 76-77 34.82856365718447 36.5 35.0 39.0 30.0 41.0 78-79 34.4425585643557 36.0 35.0 39.0 30.0 40.5 80-81 34.14872287687673 36.0 35.0 37.5 30.0 39.0 82-83 33.881041383996326 35.5 35.0 37.0 30.0 39.0 84-85 33.56324759911013 35.0 35.0 37.0 30.0 39.0 86-87 33.31705081610147 35.0 35.0 36.0 30.0 37.0 88-89 33.07175469753127 35.0 35.0 36.0 29.5 37.0 90-91 32.86000859388395 35.0 34.0 36.0 29.0 37.0 92-93 32.71245308818891 35.0 34.0 35.5 29.0 36.0 94-95 32.60586035150256 35.0 34.0 35.0 29.0 36.0 96-97 32.47265303357753 35.0 34.0 35.0 29.0 36.0 98-99 32.34812532676868 35.0 34.0 35.0 29.0 36.0 100-101 32.33031312641688 35.0 34.0 35.0 29.0 36.0 102-103 31.509209849522364 34.5 32.5 35.0 26.0 35.0 104-105 32.16423843582845 35.0 34.0 35.0 29.0 35.0 106-107 32.22877723425107 35.0 34.0 35.0 29.0 35.0 108-109 32.226937804410824 35.0 34.0 35.0 29.0 35.0 110-111 32.20569016719973 35.0 34.0 35.0 29.0 35.0 112-113 32.12164367555759 35.0 34.0 35.0 29.0 35.0 114-115 32.043811874376885 35.0 34.0 35.0 28.5 35.0 116-117 31.98988313587862 35.0 34.0 35.0 27.0 35.0 118-119 31.91253923870042 35.0 34.0 35.0 27.0 35.0 120-121 31.80824631849442 35.0 34.0 35.0 27.0 35.0 122-123 31.631754189518425 35.0 34.0 35.0 25.0 35.0 124-125 29.64467994132452 33.5 29.5 34.5 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14377217864620917 1101 2 0.07057997533544125 1101 3 0.13615340497539563 1101 4 0.1317786269221628 1101 5 0.00793551506265544 1101 6 0.132409997673534 1101 7 0.11112783059828502 1101 8 0.12726207768037767 1101 9 0.08772645796221923 1101 10-11 0.15909681877226234 1101 12-13 0.15868661508635284 1101 14-15 0.29440459077380865 1101 16-17 0.3041782392968386 1101 18-19 0.3368038465353109 1101 20-21 0.3098463465176522 1101 22-23 0.2498747166925952 1101 24-25 0.2257704799498086 1101 26-27 0.2779130870420303 1101 28-29 0.16936266814678902 1101 30-31 0.20107030282072458 1101 32-33 0.26580580453165226 1101 34-35 0.16063420109822601 1101 36-37 0.22723710681172093 1101 38-39 0.11336503649542351 1101 40-41 0.21472323881356203 1101 42-43 0.21910686953989966 1101 44-45 0.059363899270181264 1101 46-47 0.03347839916533246 1101 48-49 0.017695881876214514 1101 50-51 2.077066845984632E-4 1101 52-53 0.10456375901796378 1101 54-55 0.05099868881879388 1101 56-57 0.010971404111934646 1101 58-59 0.015995098413334574 1101 60-61 -0.04190943975596895 1101 62-63 0.08131330559503169 1101 64-65 0.09757861129553191 1101 66-67 0.1438556624406644 1101 68-69 -0.022123101001497503 1101 70-71 -0.004269304717993805 1101 72-73 -0.019172861009728592 1101 74-75 0.15588635153001462 1101 76-77 0.1486689097365428 1101 78-79 0.23123593541017584 1101 80-81 0.05164510259934474 1101 82-83 0.07152755201735772 1101 84-85 0.03572243648224571 1101 86-87 0.10219510207539173 1101 88-89 0.025909139632801725 1101 90-91 -0.03153308189396142 1101 92-93 -0.11110364550293639 1101 94-95 -0.09962542193299839 1101 96-97 -0.10098741932134914 1101 98-99 0.08018740079528186 1101 100-101 -0.03077156800872416 1101 102-103 -0.006115170285497129 1101 104-105 0.01304121262265312 1101 106-107 -0.08561025801482458 1101 108-109 -0.00604300116710732 1101 110-111 0.08704857679769873 1101 112-113 -0.05437262267445675 1101 114-115 0.05084780205285 1101 116-117 0.08226745224727239 1101 118-119 0.14757835263858254 1101 120-121 0.17909745297276558 1101 122-123 0.18732284246757658 1101 124-125 0.49213584442038893 1102 1 -0.001734417793059606 1102 2 0.07334181358805836 1102 3 0.053512809949651796 1102 4 0.07134842801932706 1102 5 -0.014127639112288648 1102 6 0.028627562375852733 1102 7 0.05957001078877511 1102 8 0.01916334985080681 1102 9 0.06153001196215513 1102 10-11 0.11205196466800516 1102 12-13 0.09787779403158936 1102 14-15 0.11215902485989915 1102 16-17 0.05590622872907147 1102 18-19 0.01706059442344099 1102 20-21 0.04596774119337965 1102 22-23 0.14607966065651823 1102 24-25 0.09059290616055193 1102 26-27 0.050941727704369555 1102 28-29 -0.06576589510835618 1102 30-31 -0.07098548925681314 1102 32-33 -0.08166310638276997 1102 34-35 -0.043885916358775034 1102 36-37 0.03226247449590147 1102 38-39 -0.10304171307546994 1102 40-41 -0.1282190339290068 1102 42-43 0.007908278014298276 1102 44-45 0.029069920203781408 1102 46-47 -0.21838526320467366 1102 48-49 -0.3009062451388971 1102 50-51 -0.1657644687941442 1102 52-53 -0.23804827106987148 1102 54-55 -0.17161115367438384 1102 56-57 -0.10774767507898986 1102 58-59 -0.26612993944888075 1102 60-61 -0.10619632596739592 1102 62-63 -0.17003711341348549 1102 64-65 -0.1495869812060704 1102 66-67 -0.19720717200890903 1102 68-69 -0.2904673442620265 1102 70-71 -0.13965196550620362 1102 72-73 -0.1474279597880397 1102 74-75 -0.24928878224980622 1102 76-77 -0.1274339324537479 1102 78-79 -0.01350121667813653 1102 80-81 -0.12540702946405702 1102 82-83 -0.046944371046627964 1102 84-85 -0.013065131249049955 1102 86-87 0.015564250259686219 1102 88-89 -0.07234436051864179 1102 90-91 0.015756824880845954 1102 92-93 -0.07342443101769192 1102 94-95 -0.08714771857083292 1102 96-97 0.04825176049892832 1102 98-99 -0.1308050520031614 1102 100-101 -0.017717517593084153 1102 102-103 0.009999409191497222 1102 104-105 -0.13071635982038288 1102 106-107 -0.22300066793640383 1102 108-109 -0.10454681263009746 1102 110-111 0.06374017643422292 1102 112-113 -0.05102076490177154 1102 114-115 0.012174704418818294 1102 116-117 -0.009524229844735999 1102 118-119 -0.05540518991854526 1102 120-121 0.04922479622319642 1102 122-123 0.06184325440847971 1102 124-125 -0.03534896862440817 1103 1 0.06566591443285574 1103 2 0.05672221225915308 1103 3 0.011253673737023462 1103 4 -0.00432432945575556 1103 5 -0.07272398462723828 1103 6 0.0469913497512664 1103 7 0.08600688786518162 1103 8 0.06457032659498907 1103 9 0.033539978739561604 1103 10-11 0.044240502873982734 1103 12-13 0.0715571960249477 1103 14-15 0.16676865941139596 1103 16-17 0.10979742474236076 1103 18-19 0.06601823561945253 1103 20-21 0.11505411993092451 1103 22-23 0.07735042477612097 1103 24-25 0.21506549752268 1103 26-27 0.10858292371765543 1103 28-29 0.06622746037336924 1103 30-31 -0.001753761682067534 1103 32-33 -0.013943006714995931 1103 34-35 0.04224780457145272 1103 36-37 0.018562308382946924 1103 38-39 -0.07140134762696704 1103 40-41 -0.048372688414055176 1103 42-43 -0.16601614059035086 1103 44-45 -0.23774071102545236 1103 46-47 -0.19745503064653747 1103 48-49 -0.1335972750392287 1103 50-51 -0.1282686216180693 1103 52-53 -0.11164876940874535 1103 54-55 -0.27976314702986826 1103 56-57 -0.19754833953081885 1103 58-59 -0.11499621851864816 1103 60-61 -0.10449782098400817 1103 62-63 -0.10113345892843739 1103 64-65 -0.14220741309975438 1103 66-67 -0.2562104942680392 1103 68-69 -0.17231950373045635 1103 70-71 -0.1357981449081933 1103 72-73 -0.14243211261196365 1103 74-75 -0.024425825439180926 1103 76-77 -0.17222213843380985 1103 78-79 -0.2849962332894336 1103 80-81 -0.35487546800226255 1103 82-83 -0.2041495205482846 1103 84-85 -0.2889953638071887 1103 86-87 -0.37879621485659243 1103 88-89 -0.47366911134920997 1103 90-91 -0.38525646415569526 1103 92-93 -0.35791778649942074 1103 94-95 -0.24784539298943997 1103 96-97 -0.4267440866771466 1103 98-99 -0.34911485266761133 1103 100-101 -0.29783379666285015 1103 102-103 -0.29577301605767303 1103 104-105 -0.3316507452024453 1103 106-107 -0.393361963617469 1103 108-109 -0.46702604851049756 1103 110-111 -0.37565351127341984 1103 112-113 -0.35711295759279693 1103 114-115 -0.23369323578051748 1103 116-117 -0.17779250226998755 1103 118-119 -0.23248160187867484 1103 120-121 -0.3065351705542092 1103 122-123 -0.23483448645530913 1103 124-125 -0.3511089354018182 1104 1 0.13840435078160596 1104 2 0.15830633014699913 1104 3 0.06117590050739352 1104 4 0.05108704999574343 1104 5 0.14845898712879801 1104 6 0.04827256137631508 1104 7 0.014328623436206556 1104 8 -0.04580216624168543 1104 9 -0.1777166729182369 1104 10-11 0.01060972104836111 1104 12-13 0.017977580797762016 1104 14-15 0.034707668826051474 1104 16-17 -0.01378833047663619 1104 18-19 0.061437801726697217 1104 20-21 0.1084802353627694 1104 22-23 0.14069467365045085 1104 24-25 0.20142243571343244 1104 26-27 0.1244241025960875 1104 28-29 0.10746159872785199 1104 30-31 0.16917951707356593 1104 32-33 0.1298985814962208 1104 34-35 0.11859450944251648 1104 36-37 0.228668734907707 1104 38-39 0.15716394095264974 1104 40-41 0.2579944830963825 1104 42-43 0.33306695396552044 1104 44-45 0.39957201922423735 1104 46-47 0.32095471147381005 1104 48-49 0.28101385881259233 1104 50-51 0.1527014970884366 1104 52-53 0.2850888482744267 1104 54-55 0.33255981653951494 1104 56-57 0.44542750983471535 1104 58-59 0.36877168979080466 1104 60-61 0.48989841315435 1104 62-63 0.39315839534909003 1104 64-65 0.3678028202199357 1104 66-67 0.3817060018432912 1104 68-69 0.46204398378488776 1104 70-71 0.6387597756812369 1104 72-73 0.6662027625415377 1104 74-75 0.5915708998985139 1104 76-77 0.3426571083938583 1104 78-79 0.41970495377564987 1104 80-81 0.3845883060460409 1104 82-83 0.52714397106039 1104 84-85 0.5091994785996903 1104 86-87 0.43563071924083374 1104 88-89 0.5446239709266862 1104 90-91 0.367584305389947 1104 92-93 0.4340429175529721 1104 94-95 0.49751277319966647 1104 96-97 0.40081014991720565 1104 98-99 0.42600385384078265 1104 100-101 0.44087680505634097 1104 102-103 0.3983223584818276 1104 104-105 0.475952611326413 1104 106-107 0.3991902263129461 1104 108-109 0.3826362519397648 1104 110-111 0.3352593008428286 1104 112-113 0.37941794703266396 1104 114-115 0.4750357859141232 1104 116-117 0.5245161346518259 1104 118-119 0.39140296627522986 1104 120-121 0.22731460431276318 1104 122-123 0.3137840153866911 1104 124-125 0.5574972864565524 1105 1 0.18275274916675244 1105 2 0.16128803858968865 1105 3 0.10800437401714191 1105 4 0.09267847166469068 1105 5 0.04674380248760457 1105 6 0.02421824050756527 1105 7 0.003681957893192589 1105 8 0.0845703265949922 1105 9 0.06463241571434963 1105 10-11 0.09790997066109952 1105 12-13 0.1300866077896501 1105 14-15 0.11879947173632388 1105 16-17 0.19640806900006424 1105 18-19 0.21991179304242792 1105 20-21 0.17264515634548872 1105 22-23 0.16348487855763238 1105 24-25 0.15646605774676914 1105 26-27 0.1381347444459493 1105 28-29 0.24554118586357276 1105 30-31 0.10303615428432522 1105 32-33 0.10747155911133177 1105 34-35 0.19976881297481697 1105 36-37 0.23763793863503935 1105 38-39 0.2951672798240068 1105 40-41 0.14152927237025636 1105 42-43 0.1511827389614666 1105 44-45 0.12232931698574845 1105 46-47 0.12824524946550753 1105 48-49 0.07117863532491242 1105 50-51 0.2424316584939774 1105 52-53 0.2854660765296302 1105 54-55 0.16027886977686023 1105 56-57 0.21060292097337907 1105 58-59 0.08011582629927716 1105 60-61 0.1900119829375626 1105 62-63 0.3076760648810861 1105 64-65 0.053296788580922794 1105 66-67 0.12633852533979706 1105 68-69 0.19322671475693198 1105 70-71 0.16463602876127226 1105 72-73 0.19721774733201158 1105 74-75 -0.010084088744505948 1105 76-77 0.15032688117403126 1105 78-79 0.1608441028450187 1105 80-81 0.0533598261153827 1105 82-83 0.05271322454974836 1105 84-85 0.035262339274048315 1105 86-87 -0.015056719058271995 1105 88-89 0.07341772338469355 1105 90-91 0.05608807365943136 1105 92-93 -0.16256764644339938 1105 94-95 0.059129396926522304 1105 96-97 0.12797580127803343 1105 98-99 0.22759383080578033 1105 100-101 0.30463118933154476 1105 102-103 0.40961914080507356 1105 104-105 0.3953100391112869 1105 106-107 0.25102178988392865 1105 108-109 0.33906638846429615 1105 110-111 0.33440251113554353 1105 112-113 0.2945817202783516 1105 114-115 0.29522833284693206 1105 116-117 0.17937836607735136 1105 118-119 0.3546332440596984 1105 120-121 0.23143401712085776 1105 122-123 0.25108988329258963 1105 124-125 0.2568042298642901 1106 1 -0.08793055921972126 1106 2 0.14033070755068167 1106 3 -0.012585236301042357 1106 4 0.051213638887261936 1106 5 -0.031359528606401454 1106 6 -0.029885027728198565 1106 7 -0.032219920389671586 1106 8 -0.08723893006700223 1106 9 -0.020497288341182696 1106 10-11 0.05347312158766471 1106 12-13 0.010674607365359634 1106 14-15 -0.10229064734075166 1106 16-17 -0.05120037129049848 1106 18-19 -0.10750009737994048 1106 20-21 -0.07838807602179543 1106 22-23 0.03016549189693052 1106 24-25 0.00699897778716263 1106 26-27 0.0018207518082178353 1106 28-29 0.03631261364927241 1106 30-31 0.04484611810154604 1106 32-33 0.27061619985683194 1106 34-35 0.15811736978884738 1106 36-37 0.22192436007599525 1106 38-39 0.3055261218180334 1106 40-41 0.14379020479371718 1106 42-43 0.27109465884863937 1106 44-45 0.2349270910182213 1106 46-47 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-0.012932664085518297 2216 72-73 -0.07163259436108405 2216 74-75 -0.04586921991759141 2216 76-77 -0.0025630685801871778 2216 78-79 0.10989259383674721 2216 80-81 0.06999760963346091 2216 82-83 -0.07149248886402404 2216 84-85 -0.16800963099266397 2216 86-87 -0.10244731732092305 2216 88-89 -0.11314289307979664 2216 90-91 -0.11857868020164375 2216 92-93 -0.0064188055840972424 2216 94-95 0.0868108975423354 2216 96-97 -3.062508424278576E-4 2216 98-99 -0.2325991958212157 2216 100-101 -0.039416157233539195 2216 102-103 -0.13492902865726464 2216 104-105 -0.1171287885598673 2216 106-107 -0.07870659952630632 2216 108-109 -0.04229471628889314 2216 110-111 0.049173244331541355 2216 112-113 0.06409419452875653 2216 114-115 -0.09374048425188874 2216 116-117 -0.10743674907376644 2216 118-119 -0.10567967488219665 2216 120-121 -0.41225964711525975 2216 122-123 -0.6073608900128384 2216 124-125 -0.7837983925076166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53.0 3 3.0 4 6.0 5 19.0 6 59.0 7 124.0 8 235.0 9 456.0 10 1199.0 11 1805.0 12 1710.0 13 2750.0 14 5042.0 15 5266.0 16 1814.0 17 1027.0 18 843.0 19 728.0 20 741.0 21 764.0 22 745.0 23 809.0 24 990.0 25 1332.0 26 1537.0 27 2092.0 28 2627.0 29 3457.0 30 4426.0 31 5738.0 32 7301.0 33 9869.0 34 14405.0 35 23467.0 36 47559.0 37 158444.0 38 160809.0 39 2178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.763345979269694 20.395806517173636 15.721749881444346 22.119097622112324 2 13.41238577648705 20.844825360001185 45.252090790362146 20.490698073149616 3 18.515798141096333 28.370188959610864 29.81146373317472 23.302549166118084 4 11.014776823607358 18.472193705297517 40.00791653348969 30.50511293760544 5 11.102021439380131 38.87657309014458 34.54885321781444 15.472552252660849 6 27.006809488833245 37.569243209032464 21.737234589747878 13.686712712386411 7 26.343217711021126 33.23208355117912 23.127284734849045 17.29741400295071 8 24.642009698811883 37.46637060807021 21.304153640017866 16.58746605310004 9 26.084977848523273 18.249726413916164 22.857826255373823 32.80746948218674 10-11 23.085902855243855 27.063008409729296 30.585019124566866 19.266069610459986 12-13 26.333798306200507 24.98534171272297 30.11669478376645 18.56416519731007 14-15 20.854033092803363 29.278050246703735 28.014579968630205 21.853336691862694 16-17 18.81956740543807 30.44894624265894 31.38654844066351 19.34493791123948 18-19 19.63162718630736 27.40168363923468 34.36325881772711 18.60343035673085 20-21 21.0062234076332 27.339069875743526 32.29948561630786 19.355221100315404 22-23 19.485635362039677 29.503362949870613 32.474718343026176 18.536283345063527 24-25 19.855999525854678 27.8710663401273 34.141426542401085 18.13150759161694 26-27 18.260310014838208 29.34917204489987 33.355170829902484 19.035347110359442 28-29 18.186648152420787 29.505491830518448 31.896644786835694 20.41121523022507 30-31 19.263423710229475 27.903981338994853 33.26859697436017 19.563997976415504 32-33 18.327727552711625 28.832374811876495 34.140368182308876 18.699529453103008 34-35 18.7419696928004 29.270747562067527 33.64198641488986 18.345296330242217 36-37 18.364029303874233 28.891325469012273 32.92346574829234 19.821179478821154 38-39 19.571406497060934 29.546767874114416 31.872302504714995 19.009523124109652 40-41 21.291770826939075 27.119207330625343 33.3842757324381 18.20474610999748 42-43 18.96697704840304 29.404524277722153 33.05734829995618 18.57115037391862 44-45 18.81287981897809 29.717269684968535 31.792290481744345 19.67756001430903 46-47 20.52562395619236 28.30382978182965 31.190295261298523 19.980251000679466 48-49 19.950193574060865 28.926463024073456 30.851196687756254 20.27214671410942 50-51 19.08360833056396 30.469552038507373 30.653177514504826 19.793662116423842 52-53 19.000738735344356 29.670913512929985 31.299412186804787 20.028935564920864 54-55 19.367883851330042 28.88698619263424 31.36534802054912 20.3797819354866 56-57 19.952627802272936 29.249580360223444 29.526764868371757 21.27102696913187 58-59 19.98395526100218 29.93613855203639 29.245029411826962 20.834876775134465 60-61 19.4458849901255 28.161586185437386 32.20092331334444 20.19160551109267 62-63 19.95580288254955 27.98632175416835 31.78033101270244 20.277544350579664 64-65 20.36432987814042 29.23275243475739 29.91232544996179 20.490592237140394 66-67 20.15943136428966 30.650849122301977 29.208833496673574 19.98088601673479 68-69 20.967171786660003 28.23090877147677 31.439115718975764 19.362803722887463 70-71 21.614041475015295 27.974785629163325 30.674556388367353 19.73661650745403 72-73 20.448998685516763 29.490992297255247 30.081451392696046 19.97855762453194 74-75 19.829752195568012 29.845754600162138 31.15748609844019 19.167007105829658 76-77 20.096353102794282 28.46512385988159 31.677987591786277 19.760535445537847 78-79 19.69830387211623 28.25260515336696 31.255913587015193 20.79317738750161 80-81 19.940350825203364 29.266619957707928 30.791611014565152 20.001418202523556 82-83 19.684651026926797 29.06881245647494 31.72709550006456 19.5194410165337 84-85 19.571829841097816 29.07029416060403 31.456896168524796 19.900979829773362 86-87 18.49981690370405 30.624390119996868 29.775585326048994 21.10020765025009 88-89 19.646867571635102 28.92910892430397 30.77689980928351 20.647123694777417 90-91 19.825878229063477 29.00706562693151 31.675302903299553 19.49175324070546 92-93 19.040850582838903 30.106852034908947 31.433083066450195 19.419214315801952 94-95 19.018322446266716 30.375021696519667 30.726293641755536 19.880362215458085 96-97 20.404335889625745 29.690810682663425 30.14918643859712 19.755666989113706 98-99 20.33698470656718 28.69894692279198 31.621633063449224 19.342435307191618 100-101 19.53874452826674 29.048870939063733 31.463207091874317 19.94917744079521 102-103 19.166730168406616 30.06563963185925 30.596937522754793 20.17069267697935 104-105 19.64669374691749 29.3209687081281 30.590381924673437 20.44195562028098 106-107 19.5612437249089 28.689563053618354 30.49765719347973 21.25153602799302 108-109 19.047810561208397 29.530170798874742 30.25282212913318 21.169196510783678 110-111 18.716190972122998 29.79597366295912 30.64628625920669 20.841549105711195 112-113 19.588206093821608 28.69676214789906 30.18100261115398 21.53402914712535 114-115 19.300098749072575 29.339376113308475 28.89495236653793 22.46557277108102 116-117 19.73026349211725 29.37774118165038 28.038795658137605 22.853199668094764 118-119 19.55284144127935 28.371654910012644 29.964915251546532 22.110588397161468 120-121 19.208217971839094 28.149792137637903 30.69176678774332 21.950223102779685 122-123 19.856569977700186 29.101096363341945 29.63155037143423 21.410783287523643 124-125 20.334317262721846 29.33986416905946 28.780516716392317 21.545301851826373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 47.5 2 130.0 3 167.0 4 58.5 5 24.5 6 59.0 7 149.5 8 258.5 9 287.5 10 229.5 11 201.0 12 224.0 13 283.5 14 372.0 15 440.0 16 529.5 17 618.5 18 717.5 19 890.5 20 1071.5 21 1294.5 22 1554.0 23 1813.5 24 2175.0 25 2678.0 26 3317.0 27 4111.0 28 5083.5 29 6229.0 30 7759.0 31 9864.5 32 12763.5 33 16772.0 34 21830.5 35 27065.0 36 30824.5 37 31419.0 38 28446.0 39 23969.5 40 20391.0 41 17989.0 42 16089.0 43 14701.5 44 13806.5 45 13260.0 46 12865.5 47 12724.0 48 13239.5 49 12877.5 50 12011.5 51 11032.0 52 9465.5 53 8341.0 54 7448.5 55 6481.5 56 5483.0 57 4550.0 58 3611.5 59 2740.5 60 2079.0 61 1558.0 62 1125.5 63 767.5 64 519.0 65 382.5 66 285.0 67 230.0 68 166.0 69 107.5 70 78.0 71 63.5 72 51.5 73 40.0 74 35.5 75 27.5 76 17.0 77 10.0 78 5.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 3.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016298745419946702 2 0.0 3 0.0 4 0.0 5 0.12425147482478847 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.0583600922043313E-4 18-19 0.0 20-21 0.004021768350376458 22-23 3.175080276612994E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.1167201844086625E-4 92-93 0.0 94-95 6.350160553225988E-4 96-97 0.0 98-99 8.46688073763465E-4 100-101 2.1167201844086625E-4 102-103 2.1167201844086625E-4 104-105 0.0012700321106451976 106-107 0.005397636470242089 108-109 4.233440368817325E-4 110-111 0.0030692442673925604 112-113 0.006879340599328153 114-115 0.004127604359596892 116-117 0.0010583600922043311 118-119 3.175080276612994E-4 120-121 2.1167201844086625E-4 122-123 7.408520645430319E-4 124-125 7.408520645430319E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 472429.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.08987197600824 #Duplication Level Percentage of deduplicated Percentage of total 1 91.87908384209493 74.50463146028406 2 5.932737482735561 9.621698458845842 3 1.013256800776301 2.464945927613098 4 0.3575038133337672 1.1595975381667971 5 0.20663111637159148 0.8377845386416007 6 0.11639711666594893 0.5663176373290976 7 0.09083674763222735 0.515615816535 8 0.05523162237083121 0.35829801496623487 9 0.03907794436379182 0.28519429549909064 >10 0.2637880093742146 4.104409220379549 >50 0.024033946357802965 1.3544921519506476 >100 0.019592888344282907 2.881843802790918 >500 0.0013061925562854518 0.7568970822825352 >1k 5.224770225141808E-4 0.5882740547155332 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1563 0.33084336482307397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 0.2569698303872116 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 885 0.18732973632016664 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 806 0.1706076468633382 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 709 0.15007546107457417 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 593 0.12552150693543368 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 580 0.12276977069570243 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 497 0.10520099316511053 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.1167201844086625E-4 0.0 10-11 0.0 0.0 0.0 3.175080276612994E-4 0.0 12-13 0.0 0.0 0.0 4.233440368817325E-4 0.0 14-15 0.0 0.0 0.0 4.233440368817325E-4 0.0 16-17 0.0 0.0 0.0 4.233440368817325E-4 0.0 18-19 0.0 0.0 0.0 4.233440368817325E-4 0.0 20-21 0.0 0.0 0.0 5.291800461021657E-4 0.0 22-23 0.0 0.0 0.0 9.525240829838981E-4 0.0 24-25 0.0 0.0 0.0 0.0014817041290860638 0.0 26-27 0.0 0.0 0.0 0.00169337614752693 0.0 28-29 0.0 0.0 0.0 0.0021167201844086627 0.0 30-31 0.0 0.0 0.0 0.004127604359596892 0.0 32-33 0.0 0.0 0.0 0.008890224774516384 0.0 34-35 0.0 0.0 0.0 0.013652845189435873 0.0 36-37 0.0 0.0 0.0 0.02370726606537702 0.0 38-39 0.0 0.0 0.0 0.044874467909463644 0.0 40-41 0.0 0.0 0.0 0.07588441861105055 0.0 42-43 0.0 0.0 0.0 0.10848190945094395 0.0 44-45 0.0 0.0 0.0 0.1373751399681222 0.0 46-47 2.1167201844086625E-4 0.0 0.0 0.17103099090021992 0.0 48-49 4.233440368817325E-4 0.0 0.0 0.2109311663763232 0.0 50-51 4.233440368817325E-4 0.0 0.0 0.2615207787836903 0.0 52-53 4.233440368817325E-4 0.0 0.0 0.30724193476691736 0.0 54-55 4.233440368817325E-4 0.0 0.0 0.3600541033679135 0.0 56-57 4.233440368817325E-4 0.0 0.0 0.42281485683563036 0.0 58-59 4.233440368817325E-4 0.0 0.0 0.4901265586998258 0.0 60-61 4.233440368817325E-4 0.0 0.0 0.5729961539194249 0.0 62-63 4.233440368817325E-4 0.0 0.0 0.658299977351094 0.0 64-65 4.233440368817325E-4 0.0 0.0 0.7448738328934084 0.0 66-67 4.233440368817325E-4 0.0 0.0 0.8417137813301047 0.0 68-69 4.233440368817325E-4 0.0 0.0 0.9653302400995705 0.0 70-71 4.233440368817325E-4 0.0 0.0 1.1029170520861336 0.0 72-73 5.291800461021657E-4 0.0 0.0 1.2362704237038793 0.0 74-75 6.350160553225988E-4 0.0 0.0 1.3737513996812218 0.0 76-77 6.350160553225988E-4 0.0 0.0 1.5242502047926778 0.0 78-79 6.350160553225988E-4 0.0 0.0 1.6910477553240804 0.0 80-81 6.350160553225988E-4 0.0 0.0 1.8770016235243814 0.0 82-83 6.350160553225988E-4 0.0 0.0 2.075232468794253 0.0 84-85 6.350160553225988E-4 0.0 0.0 2.274098330119447 0.0 86-87 6.350160553225988E-4 0.0 0.0 2.4697891111680272 0.0 88-89 7.408520645430319E-4 0.0 0.0 2.6915155504848345 0.0 90-91 0.0010583600922043311 0.0 0.0 2.934938371691831 0.0 92-93 0.0010583600922043311 0.0 0.0 3.1816421091846605 0.0 94-95 0.0010583600922043311 0.0 0.0 3.4171272297001245 0.0 96-97 0.0010583600922043311 0.0 0.0 3.665947687377362 0.0 98-99 0.0010583600922043311 0.0 0.0 3.948212323968258 0.0 100-101 0.0010583600922043311 0.0 0.0 4.24434147776703 0.0 102-103 0.0010583600922043311 0.0 0.0 4.560367801299243 0.0 104-105 0.0012700321106451976 0.0 0.0 4.87470074868393 0.0 106-107 0.0012700321106451976 0.0 0.0 5.202580705248831 0.0 108-109 0.0012700321106451976 0.0 0.0 5.54189095080954 0.0 110-111 0.0012700321106451976 0.0 0.0 5.912316983081055 0.0 112-113 0.0012700321106451976 0.0 0.0 6.334179315833702 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGACG 25 1.7155135E-5 95.30199 4 CGGACGT 45 3.152303E-4 52.945545 5 TACCTGG 720 0.0 47.981903 2 ACCTGGG 820 0.0 39.951286 3 ACGTAGA 60 0.0013008632 39.70916 1 GTACCTG 890 0.0 38.81682 1 GTATCAA 3255 0.0 38.062237 1 GTACATG 3055 0.0 33.340096 1 TACATGG 3085 0.0 32.822807 2 ATCAACG 3810 0.0 32.205067 3 TCAACGC 3885 0.0 31.583347 4 TATCAAC 3905 0.0 31.421589 2 CATGGGG 1365 0.0 31.418236 4 CAACGCA 3970 0.0 31.057163 5 ACATGGG 3085 0.0 30.892054 3 AACGCAG 4030 0.0 30.706781 6 CATGGGC 565 0.0 30.572538 4 AGCGGAA 40 0.0053558764 29.756643 104-105 TATGCGA 40 0.0053641982 29.747196 74-75 TAGGACC 755 0.0 29.190178 4 >>END_MODULE