FastQCFastQC Report
Fri 27 May 2016
SRR939935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312218
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27260.20773987249069897No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18950.14441198032643965No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC17810.13572439945192033No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA17050.12993267886890744No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16650.12688440487784805No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG16600.1265033706289656No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15480.11796820345399926No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15110.11514855001226931No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA15060.11476751576338688No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA13810.10524165954132621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC6200.01277.6769108-109
CGTCGCT954.1836756E-111261.8964110-111
CCGCGCC5450.01226.394108-109
CGTGACG502.2318342E-5990.1995108-109
CCGCGCG1950.0888.6406108-109
CGTCGCG706.1204686E-5707.2854108-109
CCTCTCG2800.0707.2854108-109
CCTCGCC7350.0673.60516108-109
CCGCGCA1903.6379788E-12651.4471108-109
CCGTTCG901.3000397E-4550.1109108-109
CCGCTCG3250.0533.1844108-109
CGGCTCG1158.932535E-6521.2181110-111
CCGCGCT1509.0381945E-7495.0998108-109
CTCCGCC4350.0482.27652110-111
CGTCGTT1251.9593282E-4479.5206110-111
CCGCACC4850.0459.37094108-109
CATCGCG1102.3721704E-4450.09067108-109
AAGCGCG1152.7101664E-4430.52155108-109
CGCGTCT1404.7025496E-6428.1434110-111
CGCCTCG1553.7329216E-4386.71017110-111
Produced by FastQC (version 0.11.5)