FastQCFastQC Report
Fri 27 May 2016
SRR939935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312218
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.23814640555151662No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28680.21856124515895986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.16666438046117338No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18200.13869646659320328No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16960.12924681722091907No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG16250.12383613088678862No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16020.12208337334192947No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15680.11949234044952896No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA14930.11377682671629256No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14830.11301475821852772No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14340.10928062257947993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.106308555438197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85300.031.0784031
TAAGGTG10350.029.3490965
TCAACGC97750.026.9930424
ATCAACG97550.026.9873283
TATCAAC98000.026.9241832
CAACGCA98900.026.6791695
GTAAGGT10600.026.4092984
AACGCAG100900.026.061496
TACATGG67850.025.6329082
GTACATG70300.025.1680091
ACATGGG67300.024.8688743
GTACTTT46700.024.7796814-15
GTACCTG12900.024.4756321
GAGTACT47800.024.27028512-13
AGTACTT50100.023.74982512-13
ACGCAGA110750.023.7436077
AGAGTAC82600.023.73250210-11
AGGTAAG11200.023.399122
CGCAGAG112300.023.3629138
GGTAAGG12300.023.2434733
Produced by FastQC (version 0.11.5)