FastQCFastQC Report
Fri 27 May 2016
SRR939934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460099
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24350.5292339257420685No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14590.31710566638919013No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA10290.22364751933822935No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC9130.19843555408727254No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8400.18256940354141174No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA7920.1721368661961882No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6960.15127179150574116No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6870.14931569075351175No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6580.14301269944077252No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5230.11367118815733136No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5210.11323649910128038No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4830.10497740703631175No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT4730.10280396175605686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC1150.02416.5815108-109
CCTCGCG550.02296.7512108-109
CCGCTCG503.6379788E-122021.1411108-109
CTCGTCG150.00202014482005.7124110-111
GTCGTTG150.00202014482005.7124110-111
CGCCGCT302.6437829E-62005.7124110-111
GTGGTCG150.00202014482005.7124110-111
CCGCGCC700.01804.5902108-109
CCGCTAG150.00286811541684.2842108-109
CCGCACG150.00286811541684.2842108-109
TTGTTCG651.4551915E-111554.7238108-109
CTCCGCT200.00359097681504.2844110-111
CGCGGAC200.00359097681504.2844110-111
CGCGTGG200.00359097681504.2844110-111
CCTCTCG357.113995E-61443.6721108-109
TAGCGTG200.00509820741263.2131108-109
AAGCGCT200.00509820741263.2131108-109
GGTCTCG200.00509820741263.2131108-109
CGGCGGT250.0056102891203.4275110-111
TGGCGCG250.0056102891203.4275110-111
Produced by FastQC (version 0.11.5)