FastQCFastQC Report
Fri 27 May 2016
SRR939934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460099
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28080.6103034346955764No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14310.31102001960447645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.2232128302821784No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9620.20908543596052154No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8970.19495804163886468No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8930.1940886635267627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.18474284882166664No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA7980.17344093336434116No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7670.16670325299555094No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7440.16170432885096467No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6330.1375790862401353No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5630.12236496927835097No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5270.11454056626943332No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5270.11454056626943332No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5030.10932429759682155No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT5010.10888960854077057No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4900.10649881873249019No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4610.10019582741975096No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGG250.00205147671.440193
CTTTCGG300.004220053659.552921
TACCTGG5350.058.9771042
CGACGTG453.1703277E-452.8841556
CGACGTC350.00777597551.0287134
TTCGACG350.00777597551.0287132
GTACCTG6500.049.474741
ACCTGGG6950.045.399643
TGACGGA558.4994826E-443.297092
GACGGAC558.4994826E-443.297093
CGGACCT758.153899E-539.6895
ACGGACC600.001304080839.6889954
CTGACGG650.001929251236.6479531
TAGCCCT4000.032.7434234
TACATGG24000.032.7434232
GTACATG24750.032.2427941
TCGAATA750.003897315131.7511984
TAGTACG750.003897315131.7511982
ACATGGG23700.031.650723
GTATAGC2900.030.8032381
Produced by FastQC (version 0.11.5)