FastQCFastQC Report
Fri 27 May 2016
SRR939933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809694
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22030.12173328750606457No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21530.11897038946915887No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19110.10559796297053535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT108.8831695E-43033.4587110-111
CTCGTCG251.518636E-62426.7673110-111
CCGCTCC5450.01754.5486108-109
CGTCGTC751.2732926E-111617.8447110-111
CCGCTCG1300.01548.5491108-109
CGGCGCC2400.01363.0457108-109
CTCCGCC3000.01314.4989110-111
TTGTTCG2500.01308.5238108-109
CCGCGCA653.0035153E-81161.4117108-109
CATCGCG704.039066E-81078.4537108-109
CGCCGCC3100.01076.3885110-111
CGTCGCC2350.01070.8052108-109
CCGCGCC5350.01034.778108-109
GTTCGCT956.473783E-8957.9343110-111
CGGCGCG651.3639918E-5774.27454108-109
CGTCGCT654.6743222E-5774.27454108-109
CCGCACG654.6743222E-5774.27454108-109
CCTCGCG2200.0686.2887108-109
CCGTTCC2400.0629.09796108-109
CCTCTCG3200.0629.09796108-109
Produced by FastQC (version 0.11.5)