##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939933_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1809694 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.788773682180523 34.0 31.0 34.0 30.0 34.0 2 31.995067121844908 34.0 31.0 34.0 30.0 34.0 3 32.065880751110406 34.0 31.0 34.0 30.0 34.0 4 35.37888394391538 37.0 35.0 37.0 33.0 37.0 5 35.41044231787252 37.0 35.0 37.0 33.0 37.0 6 35.480512727566094 37.0 37.0 37.0 35.0 37.0 7 35.403432292973285 37.0 37.0 37.0 35.0 37.0 8 35.42264106528507 37.0 37.0 37.0 35.0 37.0 9 37.105207289188115 39.0 39.0 39.0 35.0 39.0 10-11 37.079008384842965 39.0 38.5 39.0 35.0 39.0 12-13 37.02090684944527 39.0 38.0 39.0 35.0 39.0 14-15 38.54096493661359 41.0 39.5 41.0 35.0 41.0 16-17 38.5346320980232 41.0 39.0 41.0 34.5 41.0 18-19 38.53926520174129 41.0 39.5 41.0 34.0 41.0 20-21 38.57585619447266 41.0 39.5 41.0 34.0 41.0 22-23 38.55330486811582 41.0 39.0 41.0 34.0 41.0 24-25 38.56842095956554 41.0 39.0 41.0 34.0 41.0 26-27 38.52911597209252 41.0 39.0 41.0 34.0 41.0 28-29 38.47074892219348 41.0 39.0 41.0 34.0 41.0 30-31 38.39523339304877 41.0 39.0 41.0 34.0 41.0 32-33 38.30011261572399 41.0 39.0 41.0 34.0 41.0 34-35 38.23442471489655 40.0 38.5 41.0 33.0 41.0 36-37 38.40628774809443 41.0 39.0 41.0 34.0 41.0 38-39 38.37647552569661 41.0 39.0 41.0 34.0 41.0 40-41 38.31409757671739 41.0 39.0 41.0 33.0 41.0 42-43 38.250399238766335 41.0 39.0 41.0 33.0 41.0 44-45 38.16142646215327 41.0 39.0 41.0 33.0 41.0 46-47 38.06762110058386 41.0 38.0 41.0 33.0 41.0 48-49 37.98073983778473 41.0 38.0 41.0 33.0 41.0 50-51 37.87126608144803 40.0 38.0 41.0 33.0 41.0 52-53 37.768839925423855 40.0 38.0 41.0 33.0 41.0 54-55 37.629832999391056 40.0 38.0 41.0 32.5 41.0 56-57 37.453399856550334 40.0 37.5 41.0 32.0 41.0 58-59 37.297558869068475 40.0 37.0 41.0 32.0 41.0 60-61 37.0873056439376 40.0 37.0 41.0 31.0 41.0 62-63 36.846360213384145 40.0 36.0 41.0 31.0 41.0 64-65 36.56098461949921 39.0 36.0 41.0 31.0 41.0 66-67 36.26279111275166 39.0 35.0 41.0 31.0 41.0 68-69 35.88806146232457 39.0 35.0 41.0 30.0 41.0 70-71 35.48838229004461 37.5 35.0 40.0 30.0 41.0 72-73 35.09069958788613 37.0 35.0 39.5 29.0 41.0 74-75 34.67388160650364 37.0 35.0 39.0 29.0 41.0 76-77 34.21754782852791 36.0 34.5 39.0 29.0 40.5 78-79 33.76580515822012 36.0 34.0 38.0 28.5 39.5 80-81 33.33249654361455 35.0 34.0 37.0 28.0 39.0 82-83 33.11853578560795 35.0 34.0 37.0 29.0 39.0 84-85 32.69905354164848 35.0 34.0 36.5 28.0 38.0 86-87 32.062128459286484 35.0 34.0 36.0 14.5 37.0 88-89 16.49306236302933 18.0 16.5 18.5 2.0 19.0 90-91 2.772979299262748 2.0 2.0 2.0 2.0 2.0 92-93 2.2999714316342983 2.0 2.0 2.0 2.0 2.0 94-95 2.102654371402016 2.0 2.0 2.0 2.0 2.0 96-97 2.0000337073560503 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24244450698001785 1101 2 0.2916571684105662 1101 3 0.266866740161241 1101 4 0.26088736691698955 1101 5 0.24221706543026755 1101 6 0.23501239675897523 1101 7 0.26922215654587944 1101 8 0.2728734318308099 1101 9 0.2550977550302491 1101 10-11 0.21459811195003908 1101 12-13 0.29146088682161775 1101 14-15 0.31858069266696987 1101 16-17 0.35922664866649257 1101 18-19 0.27251657949503993 1101 20-21 0.29883315739647287 1101 22-23 0.3402329157716082 1101 24-25 0.3165209223200023 1101 26-27 0.34931331778027186 1101 28-29 0.4017763035904096 1101 30-31 0.3605018584793811 1101 32-33 0.39275664947967925 1101 34-35 0.47988165086147205 1101 36-37 0.46433958142950615 1101 38-39 0.4352340307461162 1101 40-41 0.4104309858434334 1101 42-43 0.38319919352250054 1101 44-45 0.41927863382206 1101 46-47 0.35419984590354403 1101 48-49 0.38469559873342263 1101 50-51 0.3131264985747677 1101 52-53 0.2734156488890207 1101 54-55 0.348504078135079 1101 56-57 0.27085903867999406 1101 58-59 0.2623993455346465 1101 60-61 0.1981739887143661 1101 62-63 0.21848600592681322 1101 64-65 0.29903410882648984 1101 66-67 0.33284766842903935 1101 68-69 0.3128108484200638 1101 70-71 0.3734905620364515 1101 72-73 0.32806754941749006 1101 74-75 0.4403587645021858 1101 76-77 0.38566582174191666 1101 78-79 0.3248884834939858 1101 80-81 0.3445240884126548 1101 82-83 0.3853091322201223 1101 84-85 0.3695095652140523 1101 86-87 0.4665445921415312 1101 88-89 1.0694565578023472 1101 90-91 -0.024736175613706557 1101 92-93 -0.1814466328316784 1101 94-95 -0.020563432452870067 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.051925450212056745 1102 2 -0.005269957616842902 1102 3 0.014237334918135502 1102 4 -0.02220695812800244 1102 5 0.02063000014425853 1102 6 -0.021767618253633714 1102 7 -0.0015676386481970894 1102 8 -0.08569019431123337 1102 9 0.0374208779043812 1102 10-11 0.10989293071517636 1102 12-13 0.0918888875376993 1102 14-15 -2.1804727882113184E-4 1102 16-17 0.04988247673127688 1102 18-19 -0.01609676413983152 1102 20-21 0.034483394991440264 1102 22-23 0.018642788290051726 1102 24-25 -0.02590650364552971 1102 26-27 -6.447536960791922E-4 1102 28-29 -0.05418437781877117 1102 30-31 -0.029476046601978112 1102 32-33 -0.02683928204019992 1102 34-35 -0.03586737787291128 1102 36-37 0.0385534608700695 1102 38-39 -0.01362816731354144 1102 40-41 -0.07005324934575441 1102 42-43 -0.0052319887362344275 1102 44-45 -0.04186359652414495 1102 46-47 -0.029855694471898175 1102 48-49 -0.08175514388825889 1102 50-51 -0.042820599249033364 1102 52-53 -0.032817410888178244 1102 54-55 -0.06375279139027157 1102 56-57 -0.083072113741963 1102 58-59 -0.056841606855286386 1102 60-61 -0.15296334211895868 1102 62-63 -0.18705703912043958 1102 64-65 -0.19162564513001712 1102 66-67 -0.12345385889987881 1102 68-69 -0.13481822704813595 1102 70-71 -0.1318956043773909 1102 72-73 -0.24360941878906317 1102 74-75 -0.14705781029861953 1102 76-77 -0.1150029931727019 1102 78-79 -0.14347620986246312 1102 80-81 -0.10956543448158129 1102 82-83 -0.11443874701316759 1102 84-85 -0.1510197863228555 1102 86-87 -0.05980874125461355 1102 88-89 0.8762043019849628 1102 90-91 0.04832922346103974 1102 92-93 -0.04044509074140823 1102 94-95 0.07812846042367827 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.033078578284616356 1103 2 0.10372925801744515 1103 3 0.13103325621642625 1103 4 0.0812197625972928 1103 5 0.014408470226044301 1103 6 -0.06383245178766117 1103 7 -0.01570279259377827 1103 8 0.056384950133576694 1103 9 0.06886179086188093 1103 10-11 0.07561329513221438 1103 12-13 0.1164606164933133 1103 14-15 0.01768243033729533 1103 16-17 0.09797597091482402 1103 18-19 0.12671763578357087 1103 20-21 0.09876271756459687 1103 22-23 0.11626916139035615 1103 24-25 0.12357224298961711 1103 26-27 0.14928049220073092 1103 28-29 0.17313115814605595 1103 30-31 0.13348007310769816 1103 32-33 0.16651008844186777 1103 34-35 0.13646522633172964 1103 36-37 0.10599740449885786 1103 38-39 0.168662751351782 1103 40-41 0.17687188975948231 1103 42-43 0.24811629541773783 1103 44-45 0.28695733241526966 1103 46-47 0.15576095323005035 1103 48-49 0.16704068903079872 1103 50-51 0.13080040849520458 1103 52-53 0.13139489076488786 1103 54-55 0.16166443224970806 1103 56-57 0.15123366736476385 1103 58-59 0.2002103196279279 1103 60-61 0.18277786506774873 1103 62-63 0.1022726412045003 1103 64-65 0.06669535193505283 1103 66-67 0.13395894946079068 1103 68-69 0.17080453355092828 1103 70-71 0.18680175934367327 1103 72-73 0.12469493352002559 1103 74-75 0.23537939441346367 1103 76-77 0.19855916873068935 1103 78-79 0.18285583092455937 1103 80-81 0.14141778451111975 1103 82-83 0.1616280640062513 1103 84-85 0.15830615629693057 1103 86-87 0.14971402928711086 1103 88-89 0.6988027803891264 1103 90-91 -0.5906305529394951 1103 92-93 -0.3275698932690543 1103 94-95 -0.11539444636937102 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.2503947986496158 1104 2 -0.06158269121218751 1104 3 0.015618437295508159 1104 4 -0.024255131749548298 1104 5 0.03150663290347211 1104 6 0.0025383386684936227 1104 7 0.033463234468584346 1104 8 -0.03665082506682893 1104 9 0.045310139261452775 1104 10-11 1.214808827825209E-4 1104 12-13 0.03837899706260117 1104 14-15 -0.05517797484018416 1104 16-17 -0.07382973861954412 1104 18-19 -0.05876524177246978 1104 20-21 -0.011612552490547046 1104 22-23 -0.09817535412123846 1104 24-25 0.03113802155387191 1104 26-27 0.03727896899493999 1104 28-29 0.061979035370455904 1104 30-31 0.009943167939688635 1104 32-33 -0.003359337525118633 1104 34-35 0.057490085618780995 1104 36-37 0.023883612971950186 1104 38-39 0.031694748149021734 1104 40-41 0.06241307198511947 1104 42-43 0.057902270790599175 1104 44-45 -0.04312097765541978 1104 46-47 8.726293249665673E-4 1104 48-49 0.06780608217423634 1104 50-51 0.002955057707779929 1104 52-53 -0.02409039715180228 1104 54-55 -0.0887818101934883 1104 56-57 -0.0691304451487369 1104 58-59 -0.006255872547114905 1104 60-61 -0.11568652200182328 1104 62-63 -0.15920440647681033 1104 64-65 -0.15588886855312722 1104 66-67 0.030295527009258194 1104 68-69 -0.04122325488692269 1104 70-71 -0.07775175540039214 1104 72-73 -0.03549914492862172 1104 74-75 -0.07431182476708642 1104 76-77 -0.07110392562752565 1104 78-79 -0.04541583389435999 1104 80-81 4.4322299540056065E-4 1104 82-83 0.006948351780806661 1104 84-85 0.00804178652036569 1104 86-87 0.07757391922779533 1104 88-89 0.48411752492643245 1104 90-91 -0.6685405748281754 1104 92-93 -0.3275698932690543 1104 94-95 -0.11539444636937102 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.06198888513997147 1105 2 0.0032654262777391807 1105 3 0.006701873134574043 1105 4 0.22205839120408655 1105 5 0.10432825227981368 1105 6 0.12036045144927243 1105 7 0.15396952480153203 1105 8 -0.0015935786767116156 1105 9 0.21240442311358265 1105 10-11 0.15467604854574546 1105 12-13 0.2715486702452736 1105 14-15 0.13913961238912975 1105 16-17 0.200039131690815 1105 18-19 0.2740176969902919 1105 20-21 0.28757829290633197 1105 22-23 0.10989129219728255 1105 24-25 0.19363295842104833 1105 26-27 0.17408639553504912 1105 28-29 0.22141084859737248 1105 30-31 0.11456513407608071 1105 32-33 0.14227070552728804 1105 34-35 0.25561348492296787 1105 36-37 0.10865659242337955 1105 38-39 0.15593384391078757 1105 40-41 0.17139040808304173 1105 42-43 0.22194698528154078 1105 44-45 0.1498145751181994 1105 46-47 0.20965679184550368 1105 48-49 0.2927726913152995 1105 50-51 0.19467925954003107 1105 52-53 0.17241143705053474 1105 54-55 0.13675522612108182 1105 56-57 0.18271623573103568 1105 58-59 0.030931547131231696 1105 60-61 0.01302885649676 1105 62-63 0.18700271897183995 1105 64-65 0.1957050429507632 1105 66-67 0.22577254296803062 1105 68-69 0.11708262087154253 1105 70-71 0.06602163828127772 1105 72-73 0.1451428918678701 1105 74-75 0.09672474625561733 1105 76-77 0.19453986390220734 1105 78-79 0.193776098713478 1105 80-81 0.05446534415258242 1105 82-83 0.037728900400651355 1105 84-85 0.11099808236108544 1105 86-87 0.13494143726573782 1105 88-89 0.7457901258333806 1105 90-91 -0.789348501657444 1105 92-93 -0.3275698932690543 1105 94-95 -0.11539444636937102 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.019657877439669846 1106 2 0.06939018015199139 1106 3 0.15390863486458528 1106 4 0.049726152719323125 1106 5 0.13727519324031334 1106 6 0.05895989582947436 1106 7 -0.048446453703355985 1106 8 0.07884717989938395 1106 9 0.18612083822052483 1106 10-11 0.060981046361277436 1106 12-13 0.09585268432137894 1106 14-15 0.19557662925694785 1106 16-17 0.07799005494955935 1106 18-19 0.14516567202947073 1106 20-21 0.17968057015342254 1106 22-23 0.07482888110831709 1106 24-25 0.05361286714679636 1106 26-27 -0.014583053164102466 1106 28-29 0.14731150257723158 1106 30-31 0.2398796676113122 1106 32-33 0.22606729831261418 1106 34-35 0.12643486569273676 1106 36-37 0.22919236806637144 1106 38-39 0.2455874762846193 1106 40-41 0.24015797250332582 1106 42-43 -0.06568052704747629 1106 44-45 0.1571661739446597 1106 46-47 -0.01804202562640711 1106 48-49 0.12277915933953665 1106 50-51 0.108934901568837 1106 52-53 0.02012528410612191 1106 54-55 0.0282935816158556 1106 56-57 -0.19497993705613936 1106 58-59 -0.07758472642606762 1106 60-61 -0.04880727383940808 1106 62-63 0.04515821171825252 1106 64-65 0.027282911840210033 1106 66-67 0.053101285054623304 1106 68-69 0.0029886308585957977 1106 70-71 0.01567808609901533 1106 72-73 0.06126189129687276 1106 74-75 0.03922832838043888 1106 76-77 0.029601983451115643 1106 78-79 0.06505852924676958 1106 80-81 -0.004618092804733465 1106 82-83 0.04920828983804881 1106 84-85 0.046541215225460064 1106 86-87 0.15876969963012755 1106 88-89 0.8180150775800286 1106 90-91 -0.23955137882454114 1106 92-93 -0.31133971990129305 1106 94-95 -0.11539444636937102 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.025743674003145145 1107 2 0.018336279494697294 1107 3 0.0016753367821706888 1107 4 0.002487638063641384 1107 5 0.03773967676335843 1107 6 0.08855417769819951 1107 7 -0.08577140083032475 1107 8 -0.01391879192588874 1107 9 0.049541416579671704 1107 10-11 0.045391396589707256 1107 12-13 0.07070378638533725 1107 14-15 0.12808686898600286 1107 16-17 0.13591258900411418 1107 18-19 0.12760711949748327 1107 20-21 0.05095047891266802 1107 22-23 0.09798252859990697 1107 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0.0 2215 124-125 0.0 2216 1 0.15049592165408043 2216 2 -0.05582215130326418 2216 3 0.00327303325472883 2216 4 0.0033196388651219877 2216 5 -0.011786565812116123 2216 6 0.005926133043203663 2216 7 0.020396195058573596 2216 8 0.003581401790199834 2216 9 -0.09420978331397123 2216 10-11 -0.0443638346111328 2216 12-13 -0.05263773278037576 2216 14-15 -0.039264484807098654 2216 16-17 -0.11744026464906199 2216 18-19 -0.15169630988660288 2216 20-21 -0.14201813595079216 2216 22-23 -0.16282361431884596 2216 24-25 -0.20225727285836115 2216 26-27 -0.20666156806308322 2216 28-29 -0.260046117771914 2216 30-31 -0.22886620872490226 2216 32-33 -0.23841271697309452 2216 34-35 -0.18201666089823476 2216 36-37 -0.20401724164313606 2216 38-39 -0.3008299299987911 2216 40-41 -0.33346424015523723 2216 42-43 -0.31906810089615334 2216 44-45 -0.28751792915627306 2216 46-47 -0.2940978792802653 2216 48-49 -0.29193266612833924 2216 50-51 -0.27317140150552177 2216 52-53 -0.22172125041647206 2216 54-55 -0.20988725335919867 2216 56-57 -0.18018058638107703 2216 58-59 -0.08551379084241972 2216 60-61 -0.12308779768810041 2216 62-63 -0.2714546943807221 2216 64-65 -0.32736981766072404 2216 66-67 -0.22462105353631046 2216 68-69 -0.24615849760773756 2216 70-71 -0.26258342773761 2216 72-73 -0.20902976824527997 2216 74-75 -0.2527371662192408 2216 76-77 -0.18460886969727142 2216 78-79 -0.15442034517044334 2216 80-81 -0.2749493508655547 2216 82-83 -0.19025422097354294 2216 84-85 -0.20787864321607685 2216 86-87 -0.25383659657972757 2216 88-89 -1.1950526037769311 2216 90-91 -0.789348501657444 2216 92-93 -0.3275698932690543 2216 94-95 -0.11539444636937102 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20707.0 3 3515.0 4 2385.0 5 1887.0 6 1947.0 7 2204.0 8 2540.0 9 2867.0 10 3550.0 11 4141.0 12 5074.0 13 6047.0 14 6672.0 15 7525.0 16 8536.0 17 9951.0 18 12666.0 19 15427.0 20 16527.0 21 22904.0 22 27212.0 23 34656.0 24 52881.0 25 87287.0 26 169391.0 27 508597.0 28 744685.0 29 26819.0 30 1094.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34931098848756 19.869491748328723 14.59003566348786 24.19116159969586 2 13.430632088254082 20.176505953525588 43.76415993068403 22.6287020275363 3 18.76432457652323 25.231708490099802 29.252706260081794 26.751260673295175 4 11.670600098515468 17.35387010442529 39.729264898806036 31.2462648982532 5 12.866008220897221 38.38230212764781 35.41608962823521 13.335600023219751 6 30.158745069608457 37.54993938201707 19.331942306268353 12.959373242106123 7 27.70540212875768 32.01259439441143 22.417878381649054 17.864125095181837 8 23.839113131833336 38.493634835502576 20.81025852989511 16.856993502768976 9 25.19066759352686 17.99613636338519 21.652831915229868 35.16036412785808 10-11 23.805343916396453 27.087684921787453 29.624287997241506 19.48268316457459 12-13 25.442845127985226 24.37861324379912 29.10322480109545 21.0753168271202 14-15 20.888586689241386 28.011310199403876 26.242005554530213 24.858097556824525 16-17 20.788720502207422 29.881103978829533 28.606438504263576 20.723737014699477 18-19 21.361450134161668 28.628865247305924 29.453784751685554 20.555899866846858 20-21 22.203194691153055 28.290475799715804 28.344242850239425 21.162086658891717 22-23 21.3162670209086 29.343267285042817 28.282337377865552 21.058128316183026 24-25 21.719307241997818 28.374410259414024 29.12774203815673 20.778540460431433 26-27 20.76478122820764 29.2992903772682 28.750302537335042 21.185625857189116 28-29 20.736567703361512 29.361281222759306 27.946056686895872 21.956094386983317 30-31 21.45735687911879 28.427071095997448 28.567619719134836 21.547952305748925 32-33 20.7606843888371 29.104097812146172 28.966365746739882 21.168852052276847 34-35 21.155555432759986 29.285058096886623 28.812629769974734 20.746756700378654 36-37 20.780121053648035 28.821261721943525 28.765147247310114 21.633469977098333 38-39 21.32559427173876 29.268042000470796 28.32453994984788 21.081823777942567 40-41 22.27995451164672 28.12282076417339 28.873279128957712 20.723945595222176 42-43 20.849685084881752 29.27271129815317 28.96039330406135 20.917210312903727 44-45 20.754868158613597 29.32888911851648 28.488940254247474 21.42730246862245 46-47 21.586909168069297 28.48042265709009 28.358716998564397 21.57395117627621 48-49 21.274034476584966 28.695269692301235 28.36239536893571 21.668300462178095 50-51 20.711684050365434 29.50342143572931 28.4881666425834 21.296727871321856 52-53 20.876015503173466 29.076545537532866 28.75262337168604 21.29481558760763 54-55 20.91548558344917 28.4772639926484 29.01600758368844 21.591242840213994 56-57 21.226019426488676 28.784175667267505 27.97834882582359 22.011456080420224 58-59 21.539332063873783 28.908975771594537 27.721786114116533 21.829906050415154 60-61 21.067429079170292 28.193164148193013 29.240053843356943 21.49935292927976 62-63 21.352449640657483 28.226899133223625 29.016065699505 21.404585526613893 64-65 21.408895647551464 28.71281001097423 28.22634655361625 21.65194778785806 66-67 21.435806274430927 29.382232576336108 27.67514839525356 21.5068127539794 68-69 21.761728606884557 28.192503848037937 28.882733133354165 21.163034411723345 70-71 22.265007233267063 28.117626515864007 28.462850625575374 21.154515625293556 72-73 21.727816967951487 28.727867805275366 28.19777818791464 21.346537038858504 74-75 21.147359719377974 29.22690244870127 28.729166367352715 20.89657146456804 76-77 21.471558175028484 28.22863975898688 28.96967664146535 21.330125424519284 78-79 21.249351753794365 28.350096328626357 28.620750343774255 21.779801573805024 80-81 21.53380626779997 28.778290694448895 28.51741786180426 21.170485175946872 82-83 21.335486931561828 28.79551656656174 28.98356039409079 20.885436107785637 84-85 21.26052802296963 28.565298884783836 29.00283141790822 21.171341674338315 86-87 20.701358559983333 29.43274021529298 28.171159051618982 21.694742173104707 88-89 30.184252622817397 18.021923036643123 34.67454612784057 17.119278212698905 90-91 30.628326595829193 14.703272523423882 16.599703819617755 38.06869706112918 92-93 22.60915196632635 17.24097843661898 35.20731312500666 24.942556472048008 94-95 15.068008284877399 6.74568595955248 27.119725277224937 51.06658047834518 96-97 25.686854891745725 6.984468587968601 14.941192626200097 52.38748389408558 98-99 57.37316906010423 14.027111458217115 18.68634545193733 9.913374029741318 100-101 30.652009732070105 6.896854038430904 12.577428519366155 49.873707710132834 102-103 22.660961683166857 1.9472682473405432 37.25428980332561 38.13748026616699 104-105 40.64483982946821 4.431912675052466 41.3087522933358 13.614495202143525 106-107 5.190389982196987 1.5577635567531156 6.372395708030602 86.8794507530193 108-109 17.881365681481736 10.578127706161293 20.96766955028893 50.57283706206805 110-111 39.87888414573612 2.6242221735667526 24.566282575554617 32.9306111051425 112-113 26.845495481202203 2.6820283676077343 38.70946711936376 31.7630090318263 114-115 27.21678567910446 3.6770546674742888 23.749531041392018 45.35662861202923 116-117 18.786058603158494 3.9841976415078797 20.047924375368616 57.181819379965006 118-119 19.336871751714188 4.086360796479188 15.267460675663159 61.309306776143465 120-121 24.610193666413444 4.055846284595621 29.51844841364361 41.81551163534733 122-123 33.64072073561698 1.519253910950662 33.65484425092549 31.185181102506863 124-125 37.86869948128026 1.8115893900449203 19.33077847811215 40.98893265056267 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11.0 1 15.5 2 34.0 3 49.5 4 60.5 5 87.5 6 99.5 7 87.5 8 82.0 9 87.5 10 89.5 11 101.5 12 116.0 13 128.0 14 176.0 15 232.5 16 310.5 17 448.5 18 659.5 19 954.0 20 1430.0 21 2195.0 22 3271.5 23 4732.0 24 6756.5 25 9223.5 26 12226.0 27 15956.0 28 20612.0 29 26098.5 30 32226.5 31 39410.5 32 47841.0 33 56734.5 34 64854.5 35 71080.5 36 74875.5 37 76543.5 38 76562.5 39 76339.0 40 75988.5 41 75002.5 42 73897.0 43 72764.0 44 71486.5 45 69888.5 46 67636.0 47 64922.0 48 62068.0 49 58737.5 50 55132.0 51 51137.0 52 46387.0 53 41332.5 54 36094.0 55 31092.5 56 26779.0 57 22615.0 58 18660.5 59 15316.0 60 12350.0 61 9667.0 62 7368.5 63 5577.0 64 4186.0 65 3026.5 66 2196.5 67 1636.0 68 1154.0 69 780.0 70 550.0 71 392.0 72 303.5 73 229.0 74 160.5 75 109.0 76 77.0 77 51.0 78 27.0 79 20.0 80 16.5 81 10.0 82 9.0 83 8.5 84 6.5 85 8.0 86 7.5 87 4.5 88 2.5 89 5.0 90 3.5 91 1.5 92 2.5 93 2.0 94 2.5 95 3.0 96 3.0 97 3.5 98 3.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.525796073811373E-4 3 0.04089089094620416 4 0.04647194498075365 5 0.04912432709618311 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.525796073811373E-4 12-13 0.0017682547436196395 14-15 0.0 16-17 1.9340286258339806E-4 18-19 0.006824358151157047 20-21 0.0019616576062030378 22-23 4.420636859049099E-4 24-25 0.0 26-27 0.0 28-29 9.117563521788767E-4 30-31 0.0 32-33 0.0011604171755003885 34-35 8.28869411071706E-5 36-37 2.7628980369056868E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.7628980369056868E-5 46-47 0.0 48-49 1.1051592147622747E-4 50-51 2.7628980369056868E-5 52-53 0.0 54-55 0.0011327881951313317 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.039187840596256E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.9340286258339806E-4 80-81 0.0 82-83 5.249506270120805E-4 84-85 0.0 86-87 0.0011327881951313317 88-89 19.27204267682824 90-91 26.268087311998606 92-93 40.346903951717806 94-95 44.73455180820625 96-97 48.49344695843607 98-99 93.06612057066002 100-101 51.783063877097455 102-103 53.18476493816081 104-105 74.88094672358973 106-107 81.62518083167652 108-109 12.579806309796021 110-111 39.05082848260535 112-113 52.110273891608195 114-115 86.81763325733522 116-117 66.45852834788644 118-119 71.96305010681364 120-121 80.39671347752714 122-123 75.9382525443528 124-125 50.081173944324284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1809694.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.17855913093368 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22116356425157 60.06783675267435 2 11.096486202863064 16.018567710778832 3 2.9105445525269538 6.302367362633509 4 1.1127310018824659 3.2126128166478654 5 0.5373591518355344 1.9392904657654741 6 0.2929498351114743 1.2686818197594494 7 0.18386298674325924 0.9289675822446889 8 0.11867447983317679 0.6852602367977398 9 0.08654280170198533 0.5621881257003075 >10 0.401115161940651 5.069910864179004 >50 0.023495967622801685 1.1535099802858348 >100 0.014003431979210754 1.9334584550431773 >500 6.119209759247046E-4 0.3100563718965644 >1k 4.589407319435282E-4 0.5472914555930628 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2203 0.12173328750606457 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2153 0.11897038946915887 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1911 0.10559796297053535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.5257960738113735E-5 0.0 5 0.0 0.0 0.0 5.5257960738113735E-5 0.0 6 0.0 0.0 0.0 5.5257960738113735E-5 0.0 7 0.0 0.0 0.0 5.5257960738113735E-5 0.0 8 0.0 0.0 0.0 5.5257960738113735E-5 0.0 9 0.0 0.0 0.0 1.657738822143412E-4 0.0 10-11 0.0 0.0 0.0 2.2103184295245494E-4 0.0 12-13 0.0 0.0 0.0 2.2103184295245494E-4 0.0 14-15 0.0 0.0 0.0 2.2103184295245494E-4 0.0 16-17 0.0 0.0 0.0 2.7628980369056867E-4 0.0 18-19 0.0 0.0 0.0 3.315477644286824E-4 0.0 20-21 0.0 0.0 0.0 3.868057251667961E-4 0.0 22-23 0.0 0.0 0.0 6.354665484883079E-4 0.0 24-25 0.0 0.0 0.0 7.459824699645355E-4 0.0 26-27 0.0 0.0 0.0 9.670143129169905E-4 0.0 28-29 0.0 0.0 0.0 0.0015472229006671847 0.0 30-31 0.0 0.0 0.0 0.00361939642834645 0.0 32-33 0.0 0.0 0.0 0.007625598581859695 0.0 34-35 0.0 0.0 0.0 0.01279221791087333 0.0 36-37 0.0 0.0 0.0 0.021992668373769265 0.0 38-39 0.0 0.0 0.0 0.03876345945778678 0.0 40-41 0.0 0.0 0.0 0.06097715967450851 0.0 42-43 0.0 0.0 0.0 0.0846275668704212 0.0 44-45 0.0 0.0 0.0 0.11023963167253689 0.0 46-47 0.0 0.0 0.0 0.13897377125635604 0.0 48-49 0.0 0.0 0.0 0.17215617667959335 0.0 50-51 0.0 0.0 0.0 0.2090408654722843 0.0 52-53 0.0 0.0 0.0 0.24614658610792767 0.0 54-55 0.0 0.0 0.0 0.2880321203474179 0.0 56-57 0.0 0.0 0.0 0.33729459234544623 0.0 58-59 0.0 0.0 0.0 0.39462472661123926 0.0 60-61 0.0 0.0 0.0 0.45883447698892743 0.0 62-63 0.0 0.0 0.0 0.5231547432880919 0.0 64-65 0.0 0.0 0.0 0.5918680174659362 0.0 66-67 0.0 0.0 0.0 0.6703066927336887 0.0 68-69 0.0 0.0 0.0 0.7621730524608028 0.0 70-71 0.0 2.7628980369056868E-5 0.0 0.8660027606877185 0.0 72-73 0.0 5.5257960738113735E-5 0.0 0.9695561791109436 0.0 74-75 0.0 5.5257960738113735E-5 0.0 1.0760106404729197 0.0 76-77 0.0 5.5257960738113735E-5 0.0 1.1930193723358755 0.0 78-79 0.0 5.5257960738113735E-5 0.0 1.2274727108560894 5.5257960738113735E-5 80-81 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 82-83 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 84-85 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 86-87 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 88-89 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 90-91 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 92-93 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 94-95 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 96-97 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 98-99 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 100-101 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 102-103 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 104-105 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 106-107 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 108-109 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 110-111 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 112-113 0.0 5.5257960738113735E-5 0.0 1.228163435365316 5.5257960738113735E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGTT 10 8.8831695E-4 3033.4587 110-111 CTCGTCG 25 1.518636E-6 2426.7673 110-111 CCGCTCC 545 0.0 1754.5486 108-109 CGTCGTC 75 1.2732926E-11 1617.8447 110-111 CCGCTCG 130 0.0 1548.5491 108-109 CGGCGCC 240 0.0 1363.0457 108-109 CTCCGCC 300 0.0 1314.4989 110-111 TTGTTCG 250 0.0 1308.5238 108-109 CCGCGCA 65 3.0035153E-8 1161.4117 108-109 CATCGCG 70 4.039066E-8 1078.4537 108-109 CGCCGCC 310 0.0 1076.3885 110-111 CGTCGCC 235 0.0 1070.8052 108-109 CCGCGCC 535 0.0 1034.778 108-109 GTTCGCT 95 6.473783E-8 957.9343 110-111 CGGCGCG 65 1.3639918E-5 774.27454 108-109 CGTCGCT 65 4.6743222E-5 774.27454 108-109 CCGCACG 65 4.6743222E-5 774.27454 108-109 CCTCGCG 220 0.0 686.2887 108-109 CCGTTCC 240 0.0 629.09796 108-109 CCTCTCG 320 0.0 629.09796 108-109 >>END_MODULE