FastQCFastQC Report
Fri 27 May 2016
SRR939933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809694
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.1927397670545407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.13068507714563898No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA22000.12156751362385022No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21860.12079390217351663No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18340.10134309999370059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86450.033.4892461
TACATGG60050.030.3516622
GTACATG61650.030.1477991
ATCAACG97900.029.2033443
TCAACGC97900.029.0816634
ACATGGG58750.028.792843
CAACGCA99600.028.5852895
TATCAAC101050.028.4698262
AACGCAG100450.028.4297526
TAGGACC18350.028.2395134
GTTCGCG450.009535382526.44230348-49
CATGGGG34650.026.3003924
AGAGTAC83750.025.82268310-11
ACGCAGA111500.025.6656327
AGTACTT54500.025.49013712-13
CGCAGAG112100.025.4751878
GGTATCA63500.024.8602921
GCAGAGT120600.023.2850139
GTCCTAC53250.023.2690051
GAGTACT53200.023.09352512-13
Produced by FastQC (version 0.11.5)