##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1809694 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84585239272496 34.0 31.0 34.0 31.0 34.0 2 32.9403556623385 34.0 33.0 34.0 31.0 34.0 3 32.97690714562793 34.0 33.0 34.0 31.0 34.0 4 36.381655130646394 37.0 37.0 37.0 35.0 37.0 5 36.276952899219424 37.0 37.0 37.0 35.0 37.0 6 36.407608689645876 37.0 37.0 37.0 35.0 37.0 7 36.40072575805633 37.0 37.0 37.0 35.0 37.0 8 36.432559869237565 37.0 37.0 37.0 35.0 37.0 9 38.273720308516246 39.0 39.0 39.0 37.0 39.0 10-11 38.267764881797696 39.0 39.0 39.0 37.0 39.0 12-13 38.25023622778215 39.0 39.0 39.0 37.0 39.0 14-15 39.80956366103883 41.0 40.0 41.0 38.0 41.0 16-17 39.826819617018124 41.0 40.0 41.0 38.0 41.0 18-19 39.85492823648639 41.0 40.0 41.0 38.0 41.0 20-21 39.86369408308808 41.0 40.0 41.0 38.0 41.0 22-23 39.81396468132182 41.0 40.0 41.0 37.5 41.0 24-25 39.78305835130138 41.0 40.0 41.0 37.0 41.0 26-27 39.70207449436203 41.0 40.0 41.0 37.0 41.0 28-29 39.625948088461364 41.0 40.0 41.0 37.0 41.0 30-31 39.52149617559654 41.0 40.0 41.0 36.5 41.0 32-33 39.40051992215258 41.0 39.5 41.0 36.0 41.0 34-35 39.320750358900455 41.0 39.0 41.0 36.0 41.0 36-37 39.31283548489413 41.0 39.5 41.0 35.5 41.0 38-39 39.378593287041895 41.0 40.0 41.0 36.0 41.0 40-41 39.31707680967058 41.0 40.0 41.0 36.0 41.0 42-43 39.231987562538194 41.0 40.0 41.0 35.0 41.0 44-45 39.10446185929776 41.0 40.0 41.0 35.0 41.0 46-47 38.92782730119015 41.0 39.5 41.0 35.0 41.0 48-49 38.748434265682484 41.0 39.0 41.0 35.0 41.0 50-51 38.60931157422194 41.0 39.0 41.0 35.0 41.0 52-53 38.50504643326441 41.0 39.0 41.0 35.0 41.0 54-55 38.40026518295358 41.0 39.0 41.0 35.0 41.0 56-57 38.265614241965764 41.0 38.5 41.0 35.0 41.0 58-59 38.11537558283334 40.5 38.0 41.0 34.0 41.0 60-61 37.94350702383939 40.0 37.5 41.0 34.0 41.0 62-63 37.73891884484338 40.0 37.0 41.0 34.0 41.0 64-65 37.49835607566804 40.0 37.0 41.0 34.0 41.0 66-67 37.2312241738106 39.0 36.0 41.0 33.5 41.0 68-69 36.90371079309541 39.0 36.0 41.0 33.0 41.0 70-71 36.54493494480282 38.5 35.0 40.5 33.0 41.0 72-73 36.18810528188743 37.0 35.0 40.0 32.5 41.0 74-75 35.81810681805874 37.0 35.0 39.0 32.0 41.0 76-77 35.42124635435604 36.5 35.0 39.0 32.0 41.0 78-79 35.03661972687095 36.0 35.0 38.5 32.0 39.5 80-81 34.73521932437197 36.0 35.0 37.0 32.0 39.0 82-83 34.47914702706645 35.0 35.0 37.0 32.0 39.0 84-85 34.19214574397661 35.0 35.0 36.5 32.0 38.0 86-87 33.96117271759756 35.0 35.0 36.0 32.0 37.0 88-89 33.749369782957785 35.0 35.0 36.0 31.0 37.0 90-91 33.573518506443634 35.0 35.0 36.0 31.0 36.5 92-93 33.43304834961049 35.0 35.0 35.0 31.0 36.0 94-95 33.33447919924584 35.0 35.0 35.0 31.0 36.0 96-97 33.23574095952134 35.0 35.0 35.0 31.0 36.0 98-99 33.14642254436386 35.0 34.0 35.0 31.0 36.0 100-101 33.11197998114598 35.0 34.0 35.0 31.0 36.0 102-103 32.29407181545609 34.5 33.0 35.0 29.0 35.0 104-105 32.96252985311329 35.0 34.0 35.0 30.5 35.0 106-107 33.02953151195727 35.0 34.0 35.0 31.0 35.0 108-109 33.0279914725915 35.0 34.0 35.0 31.0 35.0 110-111 33.00684480359663 35.0 34.0 35.0 31.0 35.0 112-113 32.9446458904102 35.0 34.0 35.0 31.0 35.0 114-115 32.895414639159995 35.0 34.0 35.0 31.0 35.0 116-117 32.842623393789225 35.0 34.0 35.0 31.0 35.0 118-119 32.77419193521115 35.0 34.0 35.0 31.0 35.0 120-121 32.68733995913121 35.0 34.0 35.0 30.5 35.0 122-123 32.53692889516128 35.0 34.0 35.0 30.0 35.0 124-125 30.55446942963838 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0995413708816244 1101 2 0.11700826989561364 1101 3 0.14764583201235126 1101 4 0.05518042157380165 1101 5 0.06985994238051063 1101 6 0.08823665975850759 1101 7 0.10334210381375186 1101 8 0.11341930336578798 1101 9 0.14731833734577293 1101 10-11 0.06726683658930455 1101 12-13 0.07061797920972879 1101 14-15 0.1719895022708613 1101 16-17 0.1897482432705786 1101 18-19 0.20338108301511681 1101 20-21 0.20112714940079002 1101 22-23 0.20613889664585372 1101 24-25 0.24408269769370605 1101 26-27 0.20169278715612649 1101 28-29 0.303325344357944 1101 30-31 0.2758500067385441 1101 32-33 0.2579182902392603 1101 34-35 0.35565121375630326 1101 36-37 0.26662057174307563 1101 38-39 0.21297182520916635 1101 40-41 0.15111112335819854 1101 42-43 0.14467020643775186 1101 44-45 0.1440316178516312 1101 46-47 0.071184970155592 1101 48-49 0.05264683391301617 1101 50-51 0.07979288240358784 1101 52-53 0.037411092106196975 1101 54-55 0.0490053492555802 1101 56-57 0.09877648742440925 1101 58-59 0.06749340575502316 1101 60-61 0.05031392792920286 1101 62-63 0.07463173893646058 1101 64-65 0.04906224381094404 1101 66-67 0.012058463711973388 1101 68-69 0.0048102317223737145 1101 70-71 -0.05875852186822783 1101 72-73 -0.08490927378668545 1101 74-75 -0.0652664632231108 1101 76-77 -0.05602876891291686 1101 78-79 -0.023874017253596946 1101 80-81 0.0014766941216706186 1101 82-83 -0.016715946913940627 1101 84-85 -0.0724782001686961 1101 86-87 0.007211598978649647 1101 88-89 0.038486216204674406 1101 90-91 0.053280484757060265 1101 92-93 0.06717498125070875 1101 94-95 0.018197039202945575 1101 96-97 0.022623987750591823 1101 98-99 0.060615905029507644 1101 100-101 -4.773853187742816E-4 1101 102-103 0.044828209344949244 1101 104-105 0.047135069181344136 1101 106-107 0.0386140791862033 1101 108-109 0.007336377662944926 1101 110-111 0.03171213390196925 1101 112-113 0.02214974986474516 1101 114-115 0.08306939764599264 1101 116-117 0.11538674715153263 1101 118-119 0.14055765079881866 1101 120-121 0.16983737752633488 1101 122-123 0.06693197220546665 1101 124-125 0.11177235396116814 1102 1 0.0726158780251751 1102 2 0.12013563524538284 1102 3 0.09663091048307848 1102 4 0.10828061975281145 1102 5 -0.058474003391928875 1102 6 0.012245843204439666 1102 7 -0.01972989075910192 1102 8 -0.1031426205096011 1102 9 -0.05535880674073468 1102 10-11 0.0896336624901437 1102 12-13 0.05476993948599329 1102 14-15 0.08184074238154437 1102 16-17 0.13116828541895842 1102 18-19 0.08131765002694635 1102 20-21 0.0883102121616659 1102 22-23 0.10149048380536385 1102 24-25 0.09159658749863553 1102 26-27 0.060085322936700436 1102 28-29 0.087297420275263 1102 30-31 0.06443900984471185 1102 32-33 0.10700088786048667 1102 34-35 0.13849914983650535 1102 36-37 0.12809077108415323 1102 38-39 0.08011399588180979 1102 40-41 0.05443776697202907 1102 42-43 0.07783676789051697 1102 44-45 0.16094412385825052 1102 46-47 0.1106700187895413 1102 48-49 0.08460045185780274 1102 50-51 0.15103046480805915 1102 52-53 0.18446777256059477 1102 54-55 0.09798791515559913 1102 56-57 0.04211558396679038 1102 58-59 0.09458527790286553 1102 60-61 0.10190752589904406 1102 62-63 0.13319206732944622 1102 64-65 0.14297483461681537 1102 66-67 0.1111595130243117 1102 68-69 0.06446439206835919 1102 70-71 0.10624694297305126 1102 72-73 0.05119149914287391 1102 74-75 0.11525451831263922 1102 76-77 0.12553131764514802 1102 78-79 0.18221095788765496 1102 80-81 0.23115235265074574 1102 82-83 0.21876453512274452 1102 84-85 0.25045945038925765 1102 86-87 0.23420610901165873 1102 88-89 0.13810885949187224 1102 90-91 0.21994809363773982 1102 92-93 0.21456269289553376 1102 94-95 0.19485643511812611 1102 96-97 0.23321015479257312 1102 98-99 0.16429894109432297 1102 100-101 0.18935855951773561 1102 102-103 0.14510584840788 1102 104-105 0.20989091954519523 1102 106-107 0.12715086793240005 1102 108-109 0.16753034812138878 1102 110-111 0.1525610530254582 1102 112-113 0.1652105006836848 1102 114-115 0.19150307677187328 1102 116-117 0.1542381568607425 1102 118-119 0.13034397236390305 1102 120-121 0.1441823032327605 1102 122-123 0.15948879556232498 1102 124-125 0.14331540214913474 1103 1 0.0035281726815483694 1103 2 0.041346467593655234 1103 3 0.08566820283790832 1103 4 0.049273670935434666 1103 5 -0.050039310123111136 1103 6 0.03766039705501356 1103 7 0.02800778494066236 1103 8 0.04333491459352956 1103 9 0.13530834601172614 1103 10-11 0.14152378132339294 1103 12-13 0.07837640148107994 1103 14-15 0.0816027628781626 1103 16-17 0.07334340887370416 1103 18-19 0.08481277259789977 1103 20-21 0.07952660503682552 1103 22-23 0.02096779427793649 1103 24-25 0.1420763210352476 1103 26-27 0.07932759988040061 1103 28-29 0.13764182310781337 1103 30-31 -0.0012939670257097191 1103 32-33 0.01847067136818481 1103 34-35 0.037254291482646806 1103 36-37 0.11642655325879758 1103 38-39 0.056569215228023495 1103 40-41 0.02087575236736683 1103 42-43 0.02799179979910349 1103 44-45 -0.05361166001032558 1103 46-47 0.07572339616519486 1103 48-49 -0.0022919869303734686 1103 50-51 0.04867948508741193 1103 52-53 -0.027079895148212074 1103 54-55 0.10215923093828394 1103 56-57 0.039748217262939534 1103 58-59 -0.03344355214018435 1103 60-61 -0.05973533361111549 1103 62-63 -0.11976219273381616 1103 64-65 -0.10156360482683624 1103 66-67 -0.05083272065038358 1103 68-69 -0.046617719606175 1103 70-71 0.050909113346129686 1103 72-73 -0.07007033538909724 1103 74-75 -0.029360608529977128 1103 76-77 -0.027304919314318 1103 78-79 -0.029275949096877696 1103 80-81 -0.12808162032730053 1103 82-83 -0.04572873884919204 1103 84-85 -0.039478297486880365 1103 86-87 -0.009350952673365498 1103 88-89 -0.00978090214196925 1103 90-91 -0.00855641922696293 1103 92-93 0.005562372520806491 1103 94-95 0.10560491517940562 1103 96-97 0.06297195434110847 1103 98-99 0.008636870147888942 1103 100-101 0.07893008593759987 1103 102-103 0.04282345465630044 1103 104-105 0.041542285556751324 1103 106-107 0.015265978203913733 1103 108-109 0.0642187045253877 1103 110-111 0.142626214299149 1103 112-113 0.12910297997900244 1103 114-115 0.09829382420242894 1103 116-117 0.06266345983580379 1103 118-119 0.05681751198910234 1103 120-121 0.04205365252590809 1103 122-123 0.0560740428584694 1103 124-125 -0.00181480992420191 1104 1 0.0360076342768636 1104 2 0.05072229671034023 1104 3 0.08289273474231607 1104 4 0.019581818072850865 1104 5 0.016088667590231864 1104 6 0.00861969690150488 1104 7 -0.03242712229302214 1104 8 0.004481618499376339 1104 9 -0.07487184473248476 1104 10-11 0.04206901437624566 1104 12-13 0.05934491636726591 1104 14-15 0.026242641969261626 1104 16-17 0.008124646436947103 1104 18-19 0.030810182200490033 1104 20-21 0.11256409177095605 1104 22-23 0.0975214701075231 1104 24-25 0.1279188575637562 1104 26-27 0.08976306972898129 1104 28-29 0.0893477010713184 1104 30-31 0.09809269390820674 1104 32-33 0.061072611264336274 1104 34-35 0.019772810395977558 1104 36-37 0.030618898853582266 1104 38-39 0.05056448570957173 1104 40-41 0.025893981089858187 1104 42-43 0.02553850275739933 1104 44-45 0.01693891492286781 1104 46-47 0.01578906812873271 1104 48-49 -0.05286529150519925 1104 50-51 0.007478900441704184 1104 52-53 0.003916632037338275 1104 54-55 0.015414970794637384 1104 56-57 0.018572725241412513 1104 58-59 0.021067464451625995 1104 60-61 0.03265373057672605 1104 62-63 0.08407954902713755 1104 64-65 0.06762949881748881 1104 66-67 0.1933308901290829 1104 68-69 0.1918132895946627 1104 70-71 0.17530326594150125 1104 72-73 0.17524989316284234 1104 74-75 0.10411177951557704 1104 76-77 0.052951798899719904 1104 78-79 0.09485984481788279 1104 80-81 -0.004470094657854418 1104 82-83 0.09552372481179816 1104 84-85 0.08000378293269961 1104 86-87 0.12462912128383863 1104 88-89 0.06623133088246647 1104 90-91 0.035935166423158194 1104 92-93 -0.03687048679467608 1104 94-95 -0.021148518548109507 1104 96-97 -0.03768458762641558 1104 98-99 0.03165862735410485 1104 100-101 0.050980710617800185 1104 102-103 0.04955662240318759 1104 104-105 0.05701360285733159 1104 106-107 0.018036856546430613 1104 108-109 0.02764918072263356 1104 110-111 -0.009629071911923859 1104 112-113 0.026903392642218193 1104 114-115 0.07862836093940473 1104 116-117 0.08485461979373099 1104 118-119 0.10942304077602927 1104 120-121 0.052302571120861785 1104 122-123 0.16286930552263357 1104 124-125 0.06905586327116353 1105 1 0.02385009138509986 1105 2 0.02290446966351567 1105 3 0.01888254421935187 1105 4 0.036336915055514396 1105 5 -0.0662444699112612 1105 6 0.038843444342688827 1105 7 0.008502784442057987 1105 8 0.068642125260169 1105 9 0.09539442728711833 1105 10-11 0.09172737482769833 1105 12-13 0.1517229938340634 1105 14-15 0.12307441170773359 1105 16-17 0.09219079533493613 1105 18-19 0.0718821578607276 1105 20-21 0.11169296040603882 1105 22-23 0.15366465665113083 1105 24-25 0.08790332679826918 1105 26-27 0.11366528786881958 1105 28-29 0.12398677285133886 1105 30-31 0.08457821707756352 1105 32-33 0.10113052604535255 1105 34-35 0.1443952269073563 1105 36-37 0.16324085007309463 1105 38-39 0.10275828522213715 1105 40-41 0.10834229410563978 1105 42-43 0.08514792724126607 1105 44-45 0.13120844398553544 1105 46-47 0.15120915324673234 1105 48-49 0.13028263745832191 1105 50-51 0.12127144796917122 1105 52-53 0.026448863969555703 1105 54-55 0.020919442415845424 1105 56-57 0.15466464362084054 1105 58-59 0.15540148606503124 1105 60-61 0.22888039139904492 1105 62-63 0.25460062715026766 1105 64-65 0.2044499776263322 1105 66-67 0.1288351838951982 1105 68-69 0.21979828608345287 1105 70-71 0.22267841166792834 1105 72-73 0.10854677576470806 1105 74-75 0.09954205114046744 1105 76-77 0.12398052421802674 1105 78-79 0.1367254271039755 1105 80-81 -0.003139193540690144 1105 82-83 0.07811888302251191 1105 84-85 0.06888633374187947 1105 86-87 0.09079799928213106 1105 88-89 0.08086536247870413 1105 90-91 0.055778630994424816 1105 92-93 0.016318584067384734 1105 94-95 0.06005817947459491 1105 96-97 0.057039025562858114 1105 98-99 0.006156287125342885 1105 100-101 0.10979459215025145 1105 102-103 0.07910444878449141 1105 104-105 0.06826437165030796 1105 106-107 0.13117471087966948 1105 108-109 0.10992401330936019 1105 110-111 0.06918260651229247 1105 112-113 0.05730958498275385 1105 114-115 0.13009675551608524 1105 116-117 0.016254405334919397 1105 118-119 0.04590718700274721 1105 120-121 -0.03545005482348529 1105 122-123 -0.040868720813691084 1105 124-125 0.03304831131452701 1106 1 0.003085359623462125 1106 2 0.06703807996914435 1106 3 0.0011993907777991808 1106 4 0.013479758712165335 1106 5 -0.08783189992603013 1106 6 0.016326695713935635 1106 7 0.003244790240373163 1106 8 -0.050219446943124524 1106 9 0.045597859768598425 1106 10-11 0.08381227073471109 1106 12-13 0.04687075396657292 1106 14-15 0.026768390725450786 1106 16-17 0.02588223240108789 1106 18-19 2.1828188020123207E-4 1106 20-21 0.09589384982531612 1106 22-23 0.02173598178534064 1106 24-25 0.042917794820276356 1106 26-27 0.06824258464562405 1106 28-29 0.04151377188939165 1106 30-31 0.008597674182787785 1106 32-33 0.062047847092173924 1106 34-35 0.05414560337580099 1106 36-37 0.025837677811935578 1106 38-39 -0.043708381268679375 1106 40-41 -0.0616989949067559 1106 42-43 0.04120145788150609 1106 44-45 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4 -0.009686849729170888 2216 5 0.05625360745863617 2216 6 -0.06342415358352582 2216 7 -0.02896219405330669 2216 8 -0.03765468946590289 2216 9 -0.05501573573037888 2216 10-11 -0.10022473810892052 2216 12-13 -0.09952235993045377 2216 14-15 -0.05393015664429868 2216 16-17 -0.09956179010040245 2216 18-19 -0.15730530618581895 2216 20-21 -0.18641386107281477 2216 22-23 -0.17630920159949426 2216 24-25 -0.1535650057503517 2216 26-27 -0.12781439002073824 2216 28-29 -0.21973127134617698 2216 30-31 -0.2817139298510867 2216 32-33 -0.26106097474297485 2216 34-35 -0.21774996636689536 2216 36-37 -0.11221285647873458 2216 38-39 -0.12295148451722326 2216 40-41 -0.15582297185519423 2216 42-43 -0.13494670910986883 2216 44-45 -0.19325221529222603 2216 46-47 -0.16618196939442953 2216 48-49 -0.08582225924871523 2216 50-51 -0.18474220788518636 2216 52-53 -0.09820743297830603 2216 54-55 -0.18778950756474444 2216 56-57 -0.27600484610624676 2216 58-59 -0.18791918177730338 2216 60-61 -0.18432242497765827 2216 62-63 -0.26091719447018136 2216 64-65 -0.27361681563735374 2216 66-67 -0.2671233509674735 2216 68-69 -0.307335717558864 2216 70-71 -0.30835115371174737 2216 72-73 -0.3034869495347152 2216 74-75 -0.3631886196111367 2216 76-77 -0.4468196128985227 2216 78-79 -0.3288558763298326 2216 80-81 -0.3209302973346979 2216 82-83 -0.27456198052463776 2216 84-85 -0.2745770191282091 2216 86-87 -0.27721539794476513 2216 88-89 -0.26912092470869453 2216 90-91 -0.2009895884365065 2216 92-93 -0.20800433350975567 2216 94-95 -0.1893866802582096 2216 96-97 -0.2225716486432816 2216 98-99 -0.23070207451249303 2216 100-101 -0.24966488107631335 2216 102-103 -0.20862150149490333 2216 104-105 -0.25213048789147763 2216 106-107 -0.2512531156759934 2216 108-109 -0.23212308212707455 2216 110-111 -0.2570452632714364 2216 112-113 -0.2843867310345445 2216 114-115 -0.3157798324119838 2216 116-117 -0.35397793219777896 2216 118-119 -0.35196448491962684 2216 120-121 -0.3397355142524887 2216 122-123 -0.37875074478083093 2216 124-125 -0.49423827558667455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 142.0 3 1.0 4 28.0 5 42.0 6 94.0 7 252.0 8 515.0 9 904.0 10 1921.0 11 3068.0 12 3718.0 13 5958.0 14 10037.0 15 11097.0 16 4138.0 17 2957.0 18 2651.0 19 2447.0 20 2384.0 21 2279.0 22 2612.0 23 2786.0 24 3512.0 25 4538.0 26 6159.0 27 7590.0 28 10022.0 29 12974.0 30 16734.0 31 21450.0 32 28357.0 33 37038.0 34 53369.0 35 86699.0 36 196721.0 37 670013.0 38 588374.0 39 6113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.38787276221088 19.90509723775043 14.891919061848725 23.815110938189964 2 13.760447898926559 20.42295548308167 43.08032186656971 22.736274751422066 3 18.820143073911943 25.452535069464783 29.441938802913644 26.285383053709634 4 11.81757799937448 17.559874763357783 39.299958998593134 31.322588238674605 5 12.658043647739515 38.20159317624367 35.30943107883273 13.830932097184082 6 29.984185171636753 37.90685055042455 18.78162827527748 13.327336002661225 7 27.26803536951551 32.373318362109835 22.34029620477274 18.01835006360191 8 24.150878546317774 37.85805777109279 20.611661419002328 17.379402263587103 9 25.465133884513076 17.41587251767426 21.676648096307993 35.442345501504676 10-11 23.85604969679957 26.940162259475915 29.365157866468035 19.838630177256487 12-13 25.707964992976713 24.301815666073935 29.17064984466987 20.81956949627948 14-15 21.043253721347366 27.563997007228846 26.928060766074264 24.464688505349525 16-17 20.4296586298501 29.582815865126257 29.12425729785585 20.863268207167792 18-19 20.878888917131846 27.82470406599127 30.93898747523062 20.35741954164627 20-21 21.687624163150726 27.42130232121197 30.01536238371145 20.87571113192585 22-23 20.809139475975968 28.461591169306534 30.083113774308057 20.64615558040944 24-25 21.237430195381098 27.554437380021152 30.825377107953056 20.382755316644694 26-27 20.308378101491193 28.479759561561234 30.507975381473333 20.70388695547424 28-29 20.278897979437406 28.568918281212184 29.703999681714144 21.44818405763626 30-31 20.94906652726925 27.5838622441142 30.358309194814147 21.1087620338024 32-33 20.326336938731078 28.273509223106224 30.690989747437964 20.70916409072473 34-35 20.729802939060416 28.41519063443875 30.508141155355545 20.346865271145287 36-37 20.345373306205357 28.065628774809444 30.378036286797656 21.21096163218754 38-39 20.92602395764146 28.46036401734216 29.939509110379987 20.674102914636396 40-41 21.930392652017414 27.35976358434078 30.358502597676733 20.351341165965074 42-43 20.606826347437742 28.49376745460835 30.324463693862057 20.574942504091855 44-45 20.55662449010717 28.605195132436755 29.71767602699683 21.12050435045925 46-47 21.400330663637057 27.916128362032477 29.402235958123306 21.28130501620716 48-49 21.25497459791545 28.20957576253223 29.135367636738586 21.400082002813736 50-51 20.763289263267712 29.093868908224263 29.016673537073117 21.126168291434904 52-53 20.854658301348184 28.74563323965267 29.28030926775466 21.119399191244487 54-55 20.854768817269658 28.110967931595066 29.572844911902234 21.46141833923304 56-57 21.155178720822416 28.480395028109722 28.574637480148578 21.789788770919284 58-59 21.421743123423077 28.58499834778697 28.354793683351993 21.63846484543796 60-61 21.02615138249892 27.866727745132604 29.811227754526453 21.29589311784202 62-63 21.240966704868335 27.850233243852273 29.60682855775617 21.301971493523215 64-65 21.354742846028113 28.30384584355145 28.85109305772136 21.490318252699076 66-67 21.24759766015691 29.031482670550936 28.34882582359228 21.37209384569988 68-69 21.612438345930308 27.874463859635938 29.504877620194353 21.008220174239405 70-71 22.122496952523466 27.80820956471094 29.01628673134795 21.053006751417644 72-73 21.55345047284237 28.37595748231469 28.814014966066086 21.256577078776854 74-75 20.965616286510315 28.806776173209393 29.36877726289638 20.85883027738391 76-77 21.262268648732878 27.937734224681076 29.541292616320767 21.25870451026527 78-79 21.02648293026335 28.004872646977887 29.21286692667379 21.755777496084974 80-81 21.32960047389227 28.434392775795246 29.024879344242727 21.211127406069753 82-83 21.123654054221323 28.418671885965253 29.487968684208493 20.969705375604935 84-85 21.10290468996416 28.156113685518104 29.503965863842176 21.23701576067556 86-87 20.493243609140553 28.985563305177557 28.72223149328008 21.79896159240181 88-89 21.144320531537375 28.16970714385968 28.980120396044857 21.705851928558086 90-91 21.1849810160122 28.350517742610172 29.40208312726594 21.062418114111697 92-93 20.826145193607317 28.868637460255712 29.37405439814687 20.931162947990103 94-95 20.67303959721178 28.83335253556488 29.129205046954294 21.364402820269046 96-97 21.28246543338266 28.38468824011131 28.99639386548223 21.336452461023796 98-99 21.39100744111923 28.141919483840738 29.28439044822438 21.18268262681566 100-101 20.978911622327207 28.15264021227904 29.44984882799214 21.41859933740161 102-103 20.84336613732142 28.560412468775702 29.094703314962768 21.501518078940105 104-105 21.098223174028043 28.15546239499751 29.042314313825337 21.70400011714911 106-107 21.09250704997455 27.86399171963013 28.97781309595978 22.06568813443554 108-109 20.791615806234322 28.430212671666045 28.703822917835144 22.074348604264486 110-111 20.597401169250034 28.63513171124459 29.07878802279924 21.688679096706135 112-113 20.945479202541186 27.909069005077512 28.90627935816016 22.239172434221143 114-115 20.71883937519186 28.23738686452866 28.315829115807457 22.727944644472032 116-117 21.05145805750005 28.159999513722962 27.87071993315901 22.917822495617983 118-119 21.035508844056164 27.74419928275008 28.649741115881728 22.570550757312024 120-121 20.619075103464223 27.894822492441357 29.070497686473907 22.415604717620514 122-123 21.100825671749337 28.153516813488366 28.449398180228748 22.29625933453355 124-125 21.286655183903797 28.172527683239313 28.021533219610877 22.519283913246014 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37.0 1 28.5 2 58.5 3 84.5 4 53.0 5 47.0 6 112.0 7 314.5 8 563.0 9 662.0 10 567.5 11 500.5 12 597.0 13 711.0 14 836.5 15 997.5 16 1191.0 17 1400.5 18 1692.0 19 2084.0 20 2558.0 21 3081.5 22 3827.5 23 4924.0 24 6313.5 25 8172.5 26 10572.0 27 13470.5 28 16727.0 29 20536.0 30 25482.0 31 31621.0 32 39128.0 33 48227.5 34 58982.0 35 69636.5 36 78063.0 37 81336.0 38 78419.5 39 72728.0 40 67857.5 41 66117.5 42 65807.5 43 65257.0 44 65233.5 45 65081.0 46 64086.0 47 65011.0 48 68218.0 49 65687.5 50 59430.0 51 55045.0 52 50349.5 53 46400.5 54 42842.5 55 38256.0 56 33164.5 57 28846.0 58 25058.5 59 20699.5 60 16136.5 61 12401.5 62 9732.0 63 7291.0 64 5336.0 65 4059.5 66 2901.0 67 2170.0 68 1494.5 69 892.0 70 645.5 71 481.0 72 370.5 73 322.0 74 275.5 75 200.5 76 108.0 77 41.0 78 22.0 79 9.5 80 3.0 81 2.5 82 2.0 83 1.0 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.5 98 6.0 99 8.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014532843674123913 2 0.0 3 0.0 4 0.0 5 0.13046404530268652 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.28869411071706E-5 18-19 0.0 20-21 0.004365378898310985 22-23 3.315477644286824E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.078375681192511E-4 92-93 0.0 94-95 4.6969266627396673E-4 96-97 0.0 98-99 0.0011051592147622747 100-101 2.7628980369056868E-5 102-103 3.039187840596256E-4 104-105 0.001906399645464924 106-107 0.006244149563406852 108-109 1.657738822143412E-4 110-111 0.004006202153513246 112-113 0.008067662267764605 114-115 0.0045587817608943836 116-117 0.001436706979190957 118-119 2.2103184295245494E-4 120-121 5.802085877501942E-4 122-123 8.841273718098198E-4 124-125 0.0013814490184528433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1809694.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.45782278075554 #Duplication Level Percentage of deduplicated Percentage of total 1 82.32513024898002 58.00449437485251 2 11.669380316558454 16.44398260610625 3 3.071157265607555 6.491611630560206 4 1.196206778570044 3.3712850085450654 5 0.538011384633138 1.8953555396255268 6 0.3104047389845592 1.3122265253808452 7 0.19384151475981154 0.9560355766150007 8 0.13061790814389962 0.7362442739196707 9 0.08894963522769381 0.564047787175713 >10 0.43229087746922445 5.337943106702323 >50 0.026676647391809073 1.2983730924938182 >100 0.015607739057085644 2.1615696915493388 >500 0.0010192854553952916 0.47448697634439924 >1k 7.056591614275074E-4 0.9523438101293608 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3488 0.1927397670545407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2365 0.13068507714563898 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2200 0.12156751362385022 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2186 0.12079390217351663 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1834 0.10134309999370059 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.5257960738113735E-5 0.0 10-11 0.0 0.0 0.0 1.1051592147622747E-4 0.0 12-13 0.0 0.0 0.0 1.1051592147622747E-4 0.0 14-15 0.0 0.0 0.0 1.1051592147622747E-4 0.0 16-17 0.0 0.0 0.0 1.1051592147622747E-4 0.0 18-19 0.0 0.0 0.0 1.1051592147622747E-4 0.0 20-21 0.0 0.0 0.0 1.657738822143412E-4 0.0 22-23 0.0 0.0 0.0 3.039187840596256E-4 0.0 24-25 0.0 0.0 0.0 3.868057251667962E-4 0.0 26-27 0.0 0.0 0.0 5.802085877501942E-4 0.0 28-29 0.0 0.0 0.0 0.0011604171755003885 0.0 30-31 0.0 0.0 0.0 0.003066816820965312 0.0 32-33 0.0 0.0 0.0 0.007100647954847615 0.0 34-35 0.0 0.0 0.0 0.012377783205337476 0.0 36-37 0.0 0.0 0.0 0.021744007550447753 0.0 38-39 0.0 0.0 0.0 0.03845954067372716 0.0 40-41 0.0 0.0 0.0 0.06056272496897265 0.0 42-43 0.0 0.0 0.0 0.08415787420414722 0.0 44-45 0.0 0.0 0.0 0.10949364920257237 0.0 46-47 0.0 0.0 0.0 0.13781335408085565 0.0 48-49 0.0 0.0 0.0 0.17063658275929522 0.0 50-51 0.0 0.0 0.0 0.20718972378755746 0.0 52-53 0.0 2.7628980369056868E-5 0.0 0.24415729952135554 0.0 54-55 0.0 5.5257960738113735E-5 0.0 0.28590468885900044 0.0 56-57 0.0 5.5257960738113735E-5 0.0 0.3351671608570289 0.0 58-59 0.0 5.5257960738113735E-5 0.0 0.3921933763387622 0.0 60-61 0.0 5.5257960738113735E-5 0.0 0.4562649818146051 0.0 62-63 0.0 5.5257960738113735E-5 0.0 0.5201431844278646 0.0 64-65 0.0 5.5257960738113735E-5 0.0 0.5886077977823876 0.0 66-67 0.0 5.5257960738113735E-5 0.0 0.6669912150894018 0.0 68-69 0.0 5.5257960738113735E-5 0.0 0.7580287054054442 0.0 70-71 0.0 5.5257960738113735E-5 0.0 0.8616926397501457 0.0 72-73 0.0 5.5257960738113735E-5 0.0 0.964859252448204 0.0 74-75 0.0 5.5257960738113735E-5 0.0 1.071258455849442 0.0 76-77 0.0 5.5257960738113735E-5 0.0 1.1881566717909215 0.0 78-79 0.0 5.5257960738113735E-5 0.0 1.3221572265808474 0.0 80-81 0.0 5.5257960738113735E-5 0.0 1.4683697906938962 0.0 82-83 0.0 5.5257960738113735E-5 0.0 1.6201081508807567 0.0 84-85 0.0 5.5257960738113735E-5 0.0 1.7741120874578797 0.0 86-87 0.0 5.5257960738113735E-5 0.0 1.9346917213628383 0.0 88-89 0.0 5.5257960738113735E-5 0.0 2.118231037954483 0.0 90-91 0.0 8.28869411071706E-5 0.0 2.319701562805646 0.0 92-93 0.0 1.1051592147622747E-4 0.0 2.535014206821706 0.0 94-95 0.0 1.1051592147622747E-4 0.0 2.751238607189945 0.0 96-97 0.0 1.1051592147622747E-4 0.0 2.9760832494333296 0.0 98-99 0.0 1.1051592147622747E-4 0.0 3.221069418365757 0.0 100-101 0.0 1.1051592147622747E-4 0.0 3.490976927591073 0.0 102-103 0.0 1.1051592147622747E-4 0.0 3.775306764569038 0.0 104-105 0.0 1.1051592147622747E-4 0.0 4.060078665232907 0.0 106-107 0.0 1.1051592147622747E-4 0.0 4.35382998451672 0.0 108-109 0.0 1.1051592147622747E-4 0.0 4.6670044769999794 0.0 110-111 0.0 1.1051592147622747E-4 0.0 5.0112615723984275 0.0 112-113 0.0 1.1051592147622747E-4 0.0 5.386407867849481 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8645 0.0 33.489246 1 TACATGG 6005 0.0 30.351662 2 GTACATG 6165 0.0 30.147799 1 ATCAACG 9790 0.0 29.203344 3 TCAACGC 9790 0.0 29.081663 4 ACATGGG 5875 0.0 28.79284 3 CAACGCA 9960 0.0 28.585289 5 TATCAAC 10105 0.0 28.469826 2 AACGCAG 10045 0.0 28.429752 6 TAGGACC 1835 0.0 28.239513 4 GTTCGCG 45 0.0095353825 26.442303 48-49 CATGGGG 3465 0.0 26.300392 4 AGAGTAC 8375 0.0 25.822683 10-11 ACGCAGA 11150 0.0 25.665632 7 AGTACTT 5450 0.0 25.490137 12-13 CGCAGAG 11210 0.0 25.475187 8 GGTATCA 6350 0.0 24.860292 1 GCAGAGT 12060 0.0 23.285013 9 GTCCTAC 5325 0.0 23.269005 1 GAGTACT 5320 0.0 23.093525 12-13 >>END_MODULE