FastQCFastQC Report
Fri 27 May 2016
SRR939932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026528
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA24920.24276006109916143No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19270.18772015960597277No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12190.11874980516849028No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA10940.10657283581159015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG256.4210326E-103031.8586108-109
CGTCGGT109.251092E-42970.4019110-111
TCGTGCG100.00127929552526.5486108-109
CGTCGCT251.6104877E-62376.3215110-111
CCGCTCC1650.02296.8628108-109
CCGTTCG451.8189894E-122245.8213108-109
CCGCGCG700.02165.6133108-109
CCGCGCT252.6207963E-62021.2391108-109
CCGCTCG850.01783.4462108-109
CCGCGCC1850.01775.4126108-109
CGGCGCG304.5280685E-61684.366108-109
CTTCGCT557.850758E-91620.2194110-111
CGTCGCC800.01579.0929108-109
CTCCGCC1150.01549.775110-111
CGTCGTC200.0036996121485.2009110-111
CGACGCC357.1893483E-61443.7422108-109
CGTGTCG200.00511583971263.2743108-109
CGGCGTC501.287586E-51188.1608110-111
CGCGGGT250.005781188.1608110-111
CGTCGCG257.61712E-61188.1608110-111
Produced by FastQC (version 0.11.5)