FastQCFastQC Report
Fri 27 May 2016
SRR939932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026528
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23890.23272623834907571No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18350.17875791015929424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.1656067832538421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.13102419028024564No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12110.11797047912964868No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA10780.10501418373390693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG300.00421598159.572722
TAGGACC15800.036.1960874
TAGTACG700.00277219734.0415532
GTATCAA36950.032.248191
TACCTGG9900.031.8924662
GGTATCA26900.030.7859341
GTACCTG11150.029.9228521
TACATGG37650.029.1138952
GTACATG37750.028.881781
TCAACGC42050.028.192564
CAACGCA42250.028.0591055
ATCAACG42300.028.0259383
AACGCAG43350.027.3111426
ACATGGG37550.027.287643
TATCAAC44750.026.4915542
ACCTGGG11550.026.3048383
TAAGACG1451.3288744E-424.6507824
CGCAGAG47900.024.5926638
GAGTACT22350.024.48985512-13
ACGCAGA48200.024.4395987
Produced by FastQC (version 0.11.5)