FastQCFastQC Report
Fri 27 May 2016
SRR939931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571809
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36230.23049874380411361No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19250.12247035104137971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC3750.02583.3408108-109
CGTGACG150.00198150922030.6454110-111
CCGCTCG1000.01987.1852108-109
CTCCGCC2400.01903.7301110-111
CGTCGAG150.00298057151655.9877108-109
CCGCGCT501.10649125E-81490.3888108-109
CCGCGCC3700.01476.962108-109
CCGCGCG1450.01370.4727108-109
CGTCGCG602.2939275E-81241.9908108-109
CCGCACG401.1325945E-51241.9907108-109
CGTCGTA300.00792344751015.3227110-111
CAGCGCG755.597576E-8993.5926108-109
CCGTTCC1800.0965.9928108-109
CGGCGCC1900.0915.1511108-109
CGTCGCC1450.0856.5454108-109
CTGTTCG1501.8189894E-12827.99384108-109
CGGGTCG804.904104E-5761.49207110-111
CGCGTTT400.008897442761.49207110-111
GGTCGCC1459.0403773E-10685.2363108-109
CTTCGCT2253.6379788E-12676.88184110-111
Produced by FastQC (version 0.11.5)