FastQCFastQC Report
Fri 27 May 2016
SRR939931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571809
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38390.2442408715053801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26320.16745037087839554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19100.12151603661768064No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18470.11750791603814459No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG1004.402393E-429.7925171
GTACATG65150.027.4374661
TACATGG66700.026.0843722
ACATGGG64700.025.9697763
CATGGGG32300.025.6410394
AGTACTT45550.024.81639912-13
GTATAGC10800.024.2753831
AGAGTAC74250.024.23831710-11
GAGTACT43600.022.9925412-13
GTATCAA107850.022.9831921
GTCCTAC16500.022.3895281
GTAGGAC19950.021.802353
TAGCCCT17050.021.3171334
AGCCGTC1400.003158001221.2478757
CAACGCA114650.020.8917755
AACGCAG115650.020.7830496
ATCAACG115550.020.7806173
TCAACGC115400.020.7559974
GTACTTT48700.020.34069314-15
TATCAAC120450.020.0836472
Produced by FastQC (version 0.11.5)