FastQCFastQC Report
Fri 27 May 2016
SRR939930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473373
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49190.3338597897477421No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24140.16384174272231133No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21150.14354817144063314No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18040.12244014244865353No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA17290.11734978175926937No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15710.10662608857363341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC8050.01422.3787108-109
CCTCGCC6400.01089.0087108-109
CTCCGCC4350.01043.5835110-111
CGTCGCC4200.0948.25244108-109
CCGCGCC10850.0757.0725108-109
CGTGTCG1201.6580634E-7756.598110-111
CCGCGCA808.990093E-5622.29065108-109
TTGTTCG2850.0611.3733108-109
CGTCGCG901.2796515E-4553.1473108-109
CCGTTCC2750.0543.0901108-109
GTTGTCG1808.3807936E-7504.39868110-111
CGTCGCT1251.9043944E-4484.22278110-111
CCTCTCC17250.0447.3278108-109
TTGCGTG1402.6745503E-4432.3417110-111
CGGCGCC5250.0426.71362108-109
CGTCGTG1452.9710995E-4417.4334110-111
CCGCACC4900.0406.39392108-109
CCGCTCG3209.822543E-11388.93164108-109
TTTTTCG7000.0375.1159792-93
CTCGTCC2553.3690176E-6356.0461110-111
Produced by FastQC (version 0.11.5)