##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1473373 Sequences flagged as poor quality 0 Sequence length 125 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.717387246813942 33.0 31.0 34.0 30.0 34.0 2 31.887525426351644 34.0 31.0 34.0 30.0 34.0 3 32.05702968630482 34.0 31.0 34.0 30.0 34.0 4 35.337992483912764 37.0 35.0 37.0 33.0 37.0 5 35.38924766505155 37.0 35.0 37.0 33.0 37.0 6 35.43877551712974 37.0 37.0 37.0 35.0 37.0 7 35.39974873979637 37.0 37.0 37.0 33.0 37.0 8 35.430237285466745 37.0 37.0 37.0 35.0 37.0 9 37.137069839069945 39.0 39.0 39.0 35.0 39.0 10-11 37.11465087252176 39.0 38.5 39.0 35.0 39.0 12-13 37.06493773131447 39.0 38.0 39.0 35.0 39.0 14-15 38.57390219584586 41.0 39.0 41.0 35.0 41.0 16-17 38.56724094984773 41.0 39.5 41.0 35.0 41.0 18-19 38.54691242475599 41.0 39.0 41.0 35.0 41.0 20-21 38.565434889875135 41.0 39.0 41.0 34.5 41.0 22-23 38.534108470835285 41.0 39.0 41.0 34.0 41.0 24-25 38.51744907772845 41.0 39.0 41.0 34.0 41.0 26-27 38.462609264592196 41.0 39.0 41.0 34.0 41.0 28-29 38.40156531984772 41.0 39.0 41.0 34.0 41.0 30-31 38.33170351295972 41.0 39.0 41.0 34.0 41.0 32-33 38.21769131102579 40.5 38.5 41.0 33.5 41.0 34-35 38.12906643463671 40.0 38.0 41.0 33.0 41.0 36-37 38.295471683002205 41.0 39.0 41.0 34.0 41.0 38-39 38.27059373288366 41.0 39.0 41.0 34.0 41.0 40-41 38.21259382383144 41.0 39.0 41.0 33.0 41.0 42-43 38.08998671755217 41.0 38.5 41.0 33.0 41.0 44-45 37.95367602093971 40.5 38.0 41.0 33.0 41.0 46-47 37.82351583746953 40.0 38.0 41.0 33.0 41.0 48-49 37.70064233564752 40.0 38.0 41.0 33.0 41.0 50-51 37.53512722168792 40.0 37.5 41.0 32.5 41.0 52-53 37.36780129675242 40.0 37.0 41.0 32.0 41.0 54-55 37.14620398229097 40.0 36.5 41.0 32.0 41.0 56-57 36.90406909859214 40.0 36.0 41.0 31.0 41.0 58-59 36.66839591875241 39.0 35.5 41.0 31.0 41.0 60-61 36.356203758315104 39.0 35.0 41.0 31.0 41.0 62-63 36.06021896695542 39.0 35.0 41.0 31.0 41.0 64-65 35.70336499990159 37.5 35.0 40.5 30.0 41.0 66-67 35.34771744833114 37.0 35.0 40.0 30.0 41.0 68-69 34.95963819073649 36.5 35.0 39.5 30.0 41.0 70-71 34.547937284041446 36.0 35.0 39.0 29.0 41.0 72-73 34.14669265691715 35.5 34.0 38.5 29.0 40.5 74-75 33.764752713671285 35.0 34.0 37.0 29.0 39.5 76-77 33.383798603612256 35.0 34.0 37.0 29.0 39.0 78-79 32.9971158016334 35.0 34.0 36.5 28.0 39.0 80-81 32.64662682158557 35.0 34.0 36.0 27.0 37.0 82-83 32.55586874471027 35.0 34.0 36.0 28.5 37.0 84-85 32.271508979735614 35.0 34.0 35.0 28.5 37.0 86-87 31.74100448426841 35.0 34.0 35.0 25.5 36.0 88-89 16.50944126164929 18.0 17.0 18.5 2.0 19.0 90-91 2.7062268006811583 2.0 2.0 2.0 2.0 2.0 92-93 2.274840790485505 2.0 2.0 2.0 2.0 2.0 94-95 2.0986172544223356 2.0 2.0 2.0 2.0 2.0 96-97 2.0000305421641364 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12960942507456608 1101 2 0.19424680908789327 1101 3 0.19544765160460287 1101 4 0.24263119244834286 1101 5 0.24226699463309842 1101 6 0.210319941052326 1101 7 0.23796840635862537 1101 8 0.1452067361030629 1101 9 0.24367684323836158 1101 10-11 0.2522007045232897 1101 12-13 0.24882612747383348 1101 14-15 0.2769310924219681 1101 16-17 0.23211257840843302 1101 18-19 0.2762631075082993 1101 20-21 0.3606043644906265 1101 22-23 0.3903270776232475 1101 24-25 0.3230180530486635 1101 26-27 0.37557941116106264 1101 28-29 0.3101471522523269 1101 30-31 0.31017385269719 1101 32-33 0.33713238482036445 1101 34-35 0.38346473417239224 1101 36-37 0.33471431012235087 1101 38-39 0.3222398892208105 1101 40-41 0.3646876956079197 1101 42-43 0.33138396713659546 1101 44-45 0.3338781088773217 1101 46-47 0.3095061134526915 1101 48-49 0.36080462947472824 1101 50-51 0.4009581145298853 1101 52-53 0.3278619813229753 1101 54-55 0.3198339015991749 1101 56-57 0.2992379836613708 1101 58-59 0.2702449171299577 1101 60-61 0.1660763516469217 1101 62-63 0.19635244496074478 1101 64-65 0.27349115838814697 1101 66-67 0.20967674213417098 1101 68-69 0.24243475792921032 1101 70-71 0.28792008471398134 1101 72-73 0.28746894622504726 1101 74-75 0.21729409162240643 1101 76-77 0.173064638743341 1101 78-79 0.24880728607904956 1101 80-81 0.21858610335338824 1101 82-83 0.33426014963946216 1101 84-85 0.27032094029104314 1101 86-87 0.3562612005804162 1101 88-89 0.6758607362110283 1101 90-91 -0.10910910366142801 1101 92-93 -0.20496714754030876 1101 94-95 -0.0383775663128465 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.12077536551164414 1102 2 0.018604760530717357 1102 3 0.032100511079640626 1102 4 0.1723991297727423 1102 5 0.2324866647883468 1102 6 0.14550111800223675 1102 7 0.18253521986817134 1102 8 0.17084765292322146 1102 9 0.2555006657367258 1102 10-11 0.2312073365022158 1102 12-13 0.17148321964532443 1102 14-15 0.22931644038865784 1102 16-17 0.2613258938603238 1102 18-19 0.1510339307621038 1102 20-21 0.24440857115711623 1102 22-23 0.16210983221456132 1102 24-25 0.16789616497178628 1102 26-27 0.09560924657820635 1102 28-29 0.16067263189661674 1102 30-31 0.1808467269414038 1102 32-33 0.0932996442342926 1102 34-35 0.17177862992243575 1102 36-37 0.18448046496095571 1102 38-39 0.14471738887421992 1102 40-41 0.04101373772550687 1102 42-43 0.17132699832443876 1102 44-45 0.06961114909076827 1102 46-47 0.09193992805718665 1102 48-49 0.03818778470094486 1102 50-51 0.0636718359924302 1102 52-53 0.07358825940774238 1102 54-55 0.07191311470243278 1102 56-57 -0.033777402303385884 1102 58-59 -0.015329114032418545 1102 60-61 -0.11695516157034547 1102 62-63 -0.18415416296877396 1102 64-65 -0.14886566980568006 1102 66-67 -0.03521240422488603 1102 68-69 -0.11853064204079544 1102 70-71 0.0037745178693455728 1102 72-73 -0.17536870044099828 1102 74-75 -0.13275039978913128 1102 76-77 -0.22320494384374712 1102 78-79 -0.20656372560896585 1102 80-81 -0.21955951724875433 1102 82-83 -0.2685871592313802 1102 84-85 -0.17359760010772618 1102 86-87 0.0076356244555420005 1102 88-89 1.0430170913387862 1102 90-91 0.11192019700997502 1102 92-93 -0.11188566813367817 1102 94-95 0.029574753606573356 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.017372009657265153 1103 2 0.15794043951203207 1103 3 0.17232036094360126 1103 4 0.2168316760027693 1103 5 0.1269953353085782 1103 6 0.054978774248255036 1103 7 0.1337009413552508 1103 8 0.16764515138434177 1103 9 0.1890694744462209 1103 10-11 0.17151669614759868 1103 12-13 0.2075366304870343 1103 14-15 0.14852407394307932 1103 16-17 0.21141485729516774 1103 18-19 0.2458851605882515 1103 20-21 0.2627963206098656 1103 22-23 0.38721418441141964 1103 24-25 0.27102727722415665 1103 26-27 0.24523172109113744 1103 28-29 0.26422625217117 1103 30-31 0.33922713713841546 1103 32-33 0.30931597635473196 1103 34-35 0.2927188195411219 1103 36-37 0.26113152432697007 1103 38-39 0.1787060649307577 1103 40-41 0.2808439514198682 1103 42-43 0.286025178261859 1103 44-45 0.2596008959866012 1103 46-47 0.20117965036742902 1103 48-49 0.08865108660113918 1103 50-51 0.1550100573065052 1103 52-53 0.17327966464756628 1103 54-55 0.17896062135874757 1103 56-57 0.24416959115137615 1103 58-59 0.1689936529580507 1103 60-61 0.23707423551799423 1103 62-63 0.1913184268232868 1103 64-65 0.16532777694919787 1103 66-67 0.1365762616331878 1103 68-69 0.02382496766377784 1103 70-71 0.1683664333968764 1103 72-73 0.18792263705184808 1103 74-75 0.19214597647611953 1103 76-77 0.20134322753425238 1103 78-79 0.12457966851452795 1103 80-81 0.27896170467359127 1103 82-83 0.28521386498634627 1103 84-85 0.1587973829737379 1103 86-87 0.24771579171044067 1103 88-89 0.801853336699395 1103 90-91 -0.46977318600447404 1103 92-93 -0.30384135214138563 1103 94-95 -0.11204374350325308 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.16475137684881602 1104 2 0.03743201622947723 1104 3 0.08383729742139678 1104 4 0.07566504807896735 1104 5 -0.02033645123434269 1104 6 0.006057068451298164 1104 7 0.05395826704527451 1104 8 0.1448807971547197 1104 9 0.1029495791835231 1104 10-11 0.1628706055945699 1104 12-13 0.14471793391380317 1104 14-15 0.24087009300457396 1104 16-17 0.14165270921557038 1104 18-19 0.17011410457485 1104 20-21 0.14957411349492844 1104 22-23 -0.048170400608690045 1104 24-25 0.1333927919851945 1104 26-27 0.1855221390315691 1104 28-29 0.20606053653207823 1104 30-31 0.13666462589932848 1104 32-33 0.20095804496030212 1104 34-35 0.23587196933563348 1104 36-37 0.22548739869484535 1104 38-39 0.2107287931006141 1104 40-41 0.2111816941292659 1104 42-43 0.11966118105134882 1104 44-45 0.12067780860464694 1104 46-47 0.12428852365196263 1104 48-49 0.14945979628846118 1104 50-51 0.10712267622150051 1104 52-53 0.11620211167584671 1104 54-55 0.058334105452978235 1104 56-57 0.032640593217180935 1104 58-59 0.014463589229706031 1104 60-61 0.05519646412425061 1104 62-63 0.036669502924496555 1104 64-65 -0.047144865474670894 1104 66-67 -0.04202353485714383 1104 68-69 0.0010892319807211948 1104 70-71 -0.0024512017681885823 1104 72-73 -4.704041456946584E-4 1104 74-75 -0.012524438745003863 1104 76-77 -0.09497210718213012 1104 78-79 -0.04244244561755295 1104 80-81 -0.016464371756576668 1104 82-83 0.0316343536727004 1104 84-85 0.025845264762700992 1104 86-87 0.10219201748335749 1104 88-89 0.8112085312397213 1104 90-91 -0.547188766774565 1104 92-93 -0.30384135214138563 1104 94-95 -0.11204374350325308 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.10430622093839048 1105 2 -0.1057426630707532 1105 3 0.0740322448607671 1105 4 0.11468715681228048 1105 5 0.03295436696593157 1105 6 0.06990451516696083 1105 7 0.015403003853272423 1105 8 -0.058573989470296794 1105 9 0.04692379358250065 1105 10-11 0.011072461743061979 1105 12-13 0.05906247067599679 1105 14-15 0.03358926845294263 1105 16-17 0.060161482282403256 1105 18-19 0.0914759066288795 1105 20-21 0.033438021862714606 1105 22-23 -0.043301035268029864 1105 24-25 -0.024140250136760244 1105 26-27 0.03610369766054333 1105 28-29 -0.04565184254391852 1105 30-31 -0.07872529058263211 1105 32-33 -0.046941042087702556 1105 34-35 0.02384426968949782 1105 36-37 -0.006279053235836329 1105 38-39 0.02840270980725279 1105 40-41 0.03750597139361389 1105 42-43 0.06469870584103887 1105 44-45 -0.02204017866674235 1105 46-47 0.12502756974026852 1105 48-49 0.1510390995414781 1105 50-51 0.06706523326479896 1105 52-53 -0.006297855919328299 1105 54-55 -0.033430207759117536 1105 56-57 0.08616883078344983 1105 58-59 -0.0061351871640340505 1105 60-61 -0.02238944107217833 1105 62-63 0.06328531561595696 1105 64-65 0.15560360393732964 1105 66-67 0.18662589676218744 1105 68-69 0.2027468468990392 1105 70-71 0.0658311695738476 1105 72-73 0.10610431922509633 1105 74-75 0.02947740184917791 1105 76-77 -0.014443735675911284 1105 78-79 0.027914374734407943 1105 80-81 0.0030511112697766407 1105 82-83 0.008190001401985114 1105 84-85 0.07385398814212607 1105 86-87 0.2439099917928118 1105 88-89 0.9749796105796982 1105 90-91 -0.7346597644543795 1105 92-93 -0.30384135214138563 1105 94-95 -0.11204374350325308 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.012003984940239576 1106 2 0.07749275828646773 1106 3 0.1373161462450483 1106 4 0.19547324889203566 1106 5 0.16966991312692414 1106 6 0.11962586550230725 1106 7 0.13731233838330326 1106 8 0.13690999061753928 1106 9 0.11959963779706584 1106 10-11 0.050837409255613863 1106 12-13 0.10534032820557115 1106 14-15 0.1283064626288848 1106 16-17 0.04375570173612431 1106 18-19 0.09382373297451352 1106 20-21 0.08959313245140521 1106 22-23 0.1219385983439949 1106 24-25 0.07302994926539697 1106 26-27 -0.0191313633262169 1106 28-29 0.11079515537460338 1106 30-31 0.14251136657332353 1106 32-33 0.27923545274854433 1106 34-35 0.1595648661366198 1106 36-37 0.2638936186137073 1106 38-39 0.2954853536345894 1106 40-41 0.2811755839188663 1106 42-43 -0.09298547194059381 1106 44-45 0.20384899721529592 1106 46-47 0.11316190463152509 1106 48-49 0.19359330597426094 1106 50-51 0.1904546152549429 1106 52-53 0.12380969426693156 1106 54-55 0.14658153402864826 1106 56-57 0.051208008322845444 1106 58-59 0.17582905711614671 1106 60-61 0.20826178830957076 1106 62-63 0.2553141163557342 1106 64-65 0.23712626690510064 1106 66-67 0.19622362897803214 1106 68-69 0.19951264944152314 1106 70-71 0.18245202499475255 1106 72-73 0.23926290687883522 1106 74-75 0.06604905480222101 1106 76-77 0.12608821023111716 1106 78-79 0.19188941822959293 1106 80-81 0.198402077258109 1106 82-83 0.2512604130317513 1106 84-85 0.24792790403471798 1106 86-87 0.3238280925044599 1106 88-89 0.9345644663507713 1106 90-91 -0.2663804399450229 1106 92-93 -0.25614623575617346 1106 94-95 -0.11204374350325308 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.18568562301707914 1107 2 -0.2137957703051363 1107 3 -0.16625689193193338 1107 4 -0.16679673847434628 1107 5 -0.0828120986367864 1107 6 -0.07322491047064972 1107 7 -0.19345373370698837 1107 8 -0.12934166012237824 1107 9 -0.08713523799146117 1107 10-11 -0.14460375858833174 1107 12-13 -0.1319720454403921 1107 14-15 -0.15352831601100547 1107 16-17 -0.1237899287928741 1107 18-19 -0.12709284081194738 1107 20-21 -0.171140790450643 1107 22-23 -0.1511921488420569 1107 24-25 -0.10998792522543255 1107 26-27 -0.1768562538372649 1107 28-29 -0.1135352129460685 1107 30-31 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2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 2 17505.0 3 2985.0 4 2198.0 5 2000.0 6 2067.0 7 2141.0 8 2408.0 9 2709.0 10 3231.0 11 3695.0 12 4437.0 13 5010.0 14 5270.0 15 5732.0 16 6325.0 17 7588.0 18 9310.0 19 11427.0 20 13655.0 21 18223.0 22 22380.0 23 30779.0 24 47334.0 25 84175.0 26 191279.0 27 527586.0 28 428653.0 29 12857.0 30 414.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.20667882471038 17.27023638956327 12.92300048935334 24.600084296373016 2 15.66844016036781 19.11124414010668 39.14805787847258 26.072257821052926 3 20.46226156298974 22.720198599051006 27.543260207670727 29.27427963028853 4 13.248221055612255 15.295967861864687 35.06050006688654 36.39531101563651 5 15.138536716541967 36.80719758034397 32.825548721110735 15.228716982003332 6 34.698002474594006 34.07548529802026 16.714029644903224 14.512482582482509 7 31.456800144973474 30.739873745480605 19.161610807310844 18.641715302235077 8 27.712059335959054 34.27543466589927 18.709383163665954 19.303122834475722 9 26.972056634674313 17.213224349842164 19.016569463401325 36.798149552082194 10-11 26.615518479454526 25.54120971978449 25.94660797113548 21.896663829625496 12-13 27.203675731221487 22.311897563324678 26.589017670401155 23.89540903505268 14-15 23.504171552000596 24.251945791033837 24.23748916177002 28.006393495195546 16-17 24.486041504905767 27.69871478137166 24.557544347372822 23.25769936634975 18-19 24.360343629806184 26.785931853022337 25.627896826891718 23.22582769027976 20-21 24.793623741422977 25.7505308514902 25.449751771569435 24.006093635517384 22-23 24.583801789982328 25.562343110149442 25.518260392102032 24.335594707766194 24-25 24.97171456243599 25.797947973798895 25.12775108543458 24.102586378330535 26-27 24.305284540981813 26.139002139987632 25.441079753735135 24.114633565295414 28-29 24.007452379093973 26.6573229126721 25.492668094247485 23.842556613986446 30-31 24.165842593830618 25.83901021669326 25.386816508786303 24.608330680689818 32-33 24.003549721553867 25.985692518096453 25.520088505485777 24.4906692548639 34-35 25.029625919319763 26.003650130449063 25.053347016232152 23.913376933999018 36-37 24.550690524864052 25.70844294584124 25.261780460060322 24.47908606923438 38-39 24.11880766106071 26.114704151630306 25.536710663219704 24.229777524089283 40-41 24.32951465786328 25.849428488237535 25.63352932353179 24.187527530367394 42-43 24.28047751655555 25.81725740868063 25.1554087118469 24.746856362916926 44-45 24.308032396456834 25.812745058780173 25.262035156114788 24.617187388648208 46-47 24.446253596339826 26.310038259150943 25.00324086297224 24.24046728153699 48-49 24.650198541236882 26.179072962928903 24.945677312205376 24.22505118362884 50-51 23.89600182438651 26.209606263727014 25.67644152325414 24.217950388632335 52-53 24.69303428256117 25.987682684561207 25.02686013657098 24.292422896306636 54-55 24.049902636781916 25.532409497003083 25.58361935970364 24.834068506511358 56-57 24.457248775428898 26.153832057462704 25.33425005073393 24.05466911637447 58-59 24.858912169559236 25.518046007358624 25.145431604895705 24.47761021818643 60-61 24.330329115573583 25.650022092165393 26.064716809660553 23.95493198260047 62-63 24.278890776093622 25.846281235736377 25.580632189076425 24.294195799093576 64-65 23.955593020635657 26.086724873283867 25.402172703159824 24.55550940292065 66-67 24.03827815495465 26.203004941722156 25.11485550502147 24.64386139830172 68-69 23.988005691730937 25.735840835149187 25.704823412792006 24.571330060327867 70-71 24.045540402871506 25.8381278875071 25.49408737638059 24.622244333240804 72-73 24.30107650947859 25.506338177773042 25.72742951038196 24.465155802366407 74-75 23.950452465193813 26.20599128665993 25.41501710700549 24.42853914114077 76-77 24.283294182803676 25.71232810700345 25.472402439843815 24.531975270349058 78-79 24.29776539194553 25.969207964337492 25.496040364661106 24.236986279055877 80-81 24.18861347398113 26.180810969116443 25.54281909604696 24.087756460855466 82-83 24.312076203817863 26.015388588499317 25.574492937762518 24.09804226992031 84-85 24.14810239773038 25.9768999353524 25.449402645970387 24.42559502094684 86-87 24.251791661604514 25.941678606552536 25.64029122688181 24.166238504961132 88-89 32.89084398856586 16.69290107435679 31.028236068478737 19.38801886859861 90-91 30.41613610395254 11.854175125746815 13.619464308896603 44.11022446140404 92-93 23.279773130015048 15.180662576407912 30.855285052351327 30.684279241225713 94-95 13.246444028171487 5.726147651649527 23.480434864026712 57.54697345615227 96-97 22.944271194460967 6.126455327245303 12.50896281131598 58.42031066697775 98-99 57.40494148750048 14.118813420940088 18.514054663504528 9.962190428054898 100-101 30.859145468390857 6.314816658696423 11.184611867855706 51.64142600505701 102-103 22.27851231244302 1.7125860287626538 35.05382423561921 40.95507742317511 104-105 42.53260906238402 4.283950200749013 35.78420324572354 17.399237491143428 106-107 3.728213598187992 1.0231341380953964 4.669504395153692 90.57914786856291 108-109 16.982676798555914 7.649149367727409 16.24075853301113 59.12741530070554 110-111 39.125311460636055 2.292093751603311 20.26674637679543 38.315848410965195 112-113 26.824926991601206 2.516879654992869 35.22308593484708 35.43510741855885 114-115 24.26203719887725 3.3055387051336096 20.38972455557726 52.04269954041189 116-117 16.160721410094933 3.3219165283755245 17.12147029324456 63.39589176828498 118-119 16.363623218791346 3.5624173107093022 12.366462067153947 67.7074974033454 120-121 22.320499209377324 3.70423610954757 26.3196817418985 47.65558293917661 122-123 31.880177027150243 1.2783267988525497 30.455683595530836 36.38581257846637 124-125 36.73101849713182 1.2781825148164554 15.526435925794082 46.46436306225765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 32.0 2 72.5 3 126.0 4 188.0 5 193.0 6 187.5 7 176.5 8 145.0 9 138.0 10 129.5 11 119.5 12 116.5 13 119.0 14 118.5 15 127.0 16 148.0 17 165.5 18 231.0 19 333.5 20 468.5 21 720.5 22 1072.5 23 1607.5 24 2333.0 25 3217.0 26 4509.0 27 5794.0 28 6998.5 29 8596.0 30 10364.5 31 12147.0 32 14064.0 33 16457.5 34 18901.0 35 21426.0 36 24116.5 37 26760.0 38 30173.0 39 34293.0 40 38421.5 41 42575.0 42 47180.0 43 52872.5 44 58349.5 45 62399.0 46 64917.5 47 66608.5 48 68204.0 49 69692.0 50 70763.5 51 70062.5 52 66996.5 53 62457.0 54 57485.5 55 52471.5 56 47398.5 57 42247.0 58 36742.5 59 31253.5 60 26239.0 61 21321.0 62 16962.0 63 13370.5 64 10370.0 65 7888.0 66 5854.5 67 4226.0 68 3114.0 69 2298.0 70 1593.5 71 1086.5 72 799.0 73 633.5 74 487.5 75 356.5 76 251.5 77 171.0 78 99.5 79 52.5 80 35.5 81 23.0 82 20.0 83 25.0 84 19.0 85 12.0 86 10.5 87 8.0 88 6.0 89 8.0 90 7.0 91 5.5 92 6.0 93 3.5 94 2.5 95 2.5 96 3.5 97 6.0 98 5.5 99 2.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.78714758584554E-4 3 0.04459155963900519 4 0.04954617737667244 5 0.052057421983435284 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.751003310091878E-4 12-13 0.0022736944412582558 14-15 3.39357379292277E-5 16-17 3.39357379292277E-4 18-19 0.006888954799633223 20-21 0.0022058229653998 22-23 4.4116459307996005E-4 24-25 0.0 26-27 0.0 28-29 0.001221686565452197 30-31 0.0 32-33 0.001221686565452197 34-35 6.78714758584554E-5 36-37 6.78714758584554E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.357429517169108E-4 46-47 0.0 48-49 1.018072137876831E-4 50-51 6.78714758584554E-5 52-53 0.0 54-55 0.0016289154206029295 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.39357379292277E-5 64-65 6.78714758584554E-5 66-67 0.0 68-69 5.090360689384155E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.39357379292277E-4 80-81 0.0 82-83 5.769075447968708E-4 84-85 3.39357379292277E-5 86-87 0.001357429517169108 88-89 19.260397740422825 90-91 26.28404348389715 92-93 40.34657211717603 94-95 44.72754014088761 96-97 48.36456891771466 98-99 93.01711107777868 100-101 51.60295458108707 102-103 53.06303970549209 104-105 74.85446658789051 106-107 81.63133164514349 108-109 12.580690700861222 110-111 39.147656431874346 112-113 52.03061275047119 114-115 86.79750477306155 116-117 66.4015493700509 118-119 71.83625599220292 120-121 80.40574246982943 122-123 75.8077214663225 124-125 50.04618653932168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1473373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.57424806259923 #Duplication Level Percentage of deduplicated Percentage of total 1 69.52759747795912 29.600851822231807 2 14.717977114653442 12.532136173178282 3 5.940878931557581 7.587853600260059 4 3.064862237872507 5.219368207715085 5 1.7341793286618743 3.691569046174122 6 1.1082479519264736 2.8309693932111144 7 0.7446205352024617 2.2191161564750512 8 0.5284497535366347 1.7998680716550493 9 0.3770909350399442 1.4448926709493268 >10 1.9831094185017162 15.181638408880465 >50 0.15335643971527962 4.542078987193329 >100 0.10704568873185154 8.521693287622906 >500 0.009876197110698653 3.003863143405857 >1k 0.0027079895303528563 1.8241010310475096 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4919 0.3338597897477421 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2414 0.16384174272231133 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2115 0.14354817144063314 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1804 0.12244014244865353 No Hit CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA 1729 0.11734978175926937 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1571 0.10662608857363341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 6.78714758584554E-5 0.0 0.0 0.0 0.0 9 6.78714758584554E-5 0.0 0.0 0.0 0.0 10-11 1.018072137876831E-4 0.0 0.0 0.0 0.0 12-13 1.696786896461385E-4 0.0 0.0 0.0 0.0 14-15 2.714859034338216E-4 0.0 0.0 0.0 0.0 16-17 3.054216413630493E-4 0.0 0.0 3.39357379292277E-5 0.0 18-19 4.072288551507324E-4 0.0 0.0 1.357429517169108E-4 0.0 20-21 4.751003310091878E-4 0.0 0.0 2.036144275753662E-4 0.0 22-23 4.751003310091878E-4 0.0 0.0 3.054216413630493E-4 0.0 24-25 6.78714758584554E-4 0.0 0.0 5.429718068676432E-4 0.0 26-27 6.78714758584554E-4 0.0 0.0 7.80521972372237E-4 0.0 28-29 8.144577103014648E-4 0.0 0.0 0.0016967868964613848 0.0 30-31 8.144577103014648E-4 0.0 0.0 0.00546365380660566 0.0 32-33 8.144577103014648E-4 0.0 0.0 0.012352608606238881 0.0 34-35 8.483934482306924E-4 0.0 0.0 0.023619273598742475 0.0 36-37 8.823291861599201E-4 0.0 0.0 0.03987449206684254 0.0 38-39 0.0010520078758060585 0.0 0.0 0.06563171715512636 0.0 40-41 0.0010859436137352864 0.0 0.0 0.09332327930537618 0.0 42-43 0.0011538150895937418 0.0 0.0 0.12413692934511492 0.0 44-45 0.001221686565452197 0.0 0.0 0.1606517833569639 0.0 46-47 0.0014253009930275634 0.0 0.0 0.20385197774087077 0.0 48-49 0.0014931724688860186 0.0 0.0 0.25210859707623257 0.0 50-51 0.0014931724688860186 0.0 0.0 0.30467505512860626 0.0 52-53 0.0014931724688860186 0.0 0.0 0.3600581794291059 0.0 54-55 0.0014931724688860186 0.0 0.0 0.41835977719151907 0.0 56-57 0.0014931724688860186 0.0 0.0 0.4850774379603807 0.0 58-59 0.0014931724688860186 0.0 0.0 0.5565461020393343 0.0 60-61 0.0014931724688860186 0.0 0.0 0.6372113510971085 0.0 62-63 0.0014931724688860186 0.0 0.0 0.7129219824172155 0.0 64-65 0.0014931724688860186 0.0 0.0 0.7961324118196818 0.0 66-67 0.0014931724688860186 0.0 0.0 0.8959034813316112 0.0 68-69 0.0016967868964613848 0.0 0.0 1.0043620997534228 0.0 70-71 0.0017646583723198402 0.0 0.0 1.130467301898433 0.0 72-73 0.0019004013240367511 0.0 0.0 1.2529074443470867 0.0 74-75 0.0019682727998952066 0.0 0.0 1.3792841323955305 0.0 76-77 0.0019682727998952066 0.0 0.0 1.5189296939743024 0.0 78-79 0.0019682727998952066 0.0 0.0 1.5571413348826129 0.0 80-81 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 82-83 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 84-85 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 86-87 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 88-89 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 90-91 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 92-93 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 94-95 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 96-97 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 98-99 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 100-101 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 102-103 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 104-105 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 106-107 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 108-109 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 110-111 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 112-113 0.0019682727998952066 0.0 0.0 1.5578539853791267 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 805 0.0 1422.3787 108-109 CCTCGCC 640 0.0 1089.0087 108-109 CTCCGCC 435 0.0 1043.5835 110-111 CGTCGCC 420 0.0 948.25244 108-109 CCGCGCC 1085 0.0 757.0725 108-109 CGTGTCG 120 1.6580634E-7 756.598 110-111 CCGCGCA 80 8.990093E-5 622.29065 108-109 TTGTTCG 285 0.0 611.3733 108-109 CGTCGCG 90 1.2796515E-4 553.1473 108-109 CCGTTCC 275 0.0 543.0901 108-109 GTTGTCG 180 8.3807936E-7 504.39868 110-111 CGTCGCT 125 1.9043944E-4 484.22278 110-111 CCTCTCC 1725 0.0 447.3278 108-109 TTGCGTG 140 2.6745503E-4 432.3417 110-111 CGGCGCC 525 0.0 426.71362 108-109 CGTCGTG 145 2.9710995E-4 417.4334 110-111 CCGCACC 490 0.0 406.39392 108-109 CCGCTCG 320 9.822543E-11 388.93164 108-109 TTTTTCG 700 0.0 375.11597 92-93 CTCGTCC 255 3.3690176E-6 356.0461 110-111 >>END_MODULE