FastQCFastQC Report
Fri 27 May 2016
SRR939930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473373
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51000.3461445268781225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.20999434630606098No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25130.1705610188322984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.14504134390951917No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20240.13737186713751373No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17650.11979315489017378No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA17170.11653532404896792No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16820.11415982239392197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT4650.029.4636124
GTACATG64900.029.0096191
ACATGGG66900.027.5133483
TACATGG67700.027.4521922
CATGGGG36200.026.163734
TAAGGTG6550.025.4640715
ACCGTCG2401.3042154E-824.7887258
AGTACCG1751.735832E-523.8270955
AGTACTT40300.023.69383812-13
GTACTTT39500.023.57245314-15
GAGTACT39100.023.50808112-13
TACCTGG17200.023.2037122
TAGCGCG3005.7134457E-921.8415014
ATGGGGA21850.021.8096985
CGCGCGT3057.014023E-921.456477
GTACCTG18650.021.4040341
ATACCGT3358.913048E-1021.3109056
TAGGGCG1400.003135320521.274194
CTATAGG2556.3059997E-721.0281941
TACTTTT46650.020.6609814-15
Produced by FastQC (version 0.11.5)