FastQCFastQC Report
Fri 27 May 2016
SRR939929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512616
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20560.13592345975449158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG457.625204E-91635.7942108-109
CCGCTCC6300.01480.0043108-109
CCGCGCC6350.01468.3506108-109
CCGCTCG2100.01402.1094108-109
CGGTACG250.0055198071216.4779110-111
CGTCGCG856.730261E-111154.6782108-109
CGTGTCG803.216337E-81140.448110-111
CGTCGCC2700.0999.652108-109
CTCCGCC3950.0846.91504110-111
CGTCGCT855.9077815E-5715.5752110-111
CCGCGCG2500.0687.03357108-109
TTGTTCG2350.0626.4744108-109
CGACGCC809.3875344E-5613.42285108-109
CGGCGCC3600.0613.4228108-109
CCTCTCG3450.0568.97186108-109
CGTCTCT3850.0552.9445110-111
CCGCGCT901.3362174E-4545.2647108-109
CGGGGCG1102.4381683E-4446.1257108-109
CTGTTCG2804.9112714E-11438.15918108-109
CCGCACC3550.0414.7084108-109
Produced by FastQC (version 0.11.5)