##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939929_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1512616 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781269667913072 33.0 31.0 34.0 30.0 34.0 2 31.787234169148018 34.0 31.0 34.0 30.0 34.0 3 31.98335532613697 34.0 31.0 34.0 30.0 34.0 4 35.27528335016951 37.0 35.0 37.0 33.0 37.0 5 35.328163261528374 37.0 35.0 37.0 33.0 37.0 6 35.37089981859243 37.0 36.0 37.0 33.0 37.0 7 35.338104978395044 37.0 37.0 37.0 33.0 37.0 8 35.355881466280934 37.0 37.0 37.0 33.0 37.0 9 37.02754830042787 39.0 38.0 39.0 34.0 39.0 10-11 37.00116024159469 39.0 38.0 39.0 34.0 39.0 12-13 36.9371915939009 39.0 38.0 39.0 34.0 39.0 14-15 38.435582461113725 41.0 39.0 41.0 34.0 41.0 16-17 38.41934436763858 41.0 39.0 41.0 34.0 41.0 18-19 38.421061260756204 41.0 39.0 41.0 34.0 41.0 20-21 38.46041692009075 41.0 39.0 41.0 34.0 41.0 22-23 38.43291126102064 41.0 39.0 41.0 34.0 41.0 24-25 38.440858750667715 41.0 39.0 41.0 34.0 41.0 26-27 38.40423610486732 41.0 39.0 41.0 34.0 41.0 28-29 38.34644185966564 41.0 39.0 41.0 34.0 41.0 30-31 38.271503474774825 41.0 39.0 41.0 34.0 41.0 32-33 38.17268493788245 40.5 38.5 41.0 33.0 41.0 34-35 38.09266033150516 40.0 38.0 41.0 33.0 41.0 36-37 38.253564685286946 41.0 39.0 41.0 33.0 41.0 38-39 38.22224940103767 41.0 39.0 41.0 33.0 41.0 40-41 38.16413650258889 41.0 39.0 41.0 33.0 41.0 42-43 38.057293457162956 41.0 38.5 41.0 33.0 41.0 44-45 37.93234337069025 40.5 38.0 41.0 33.0 41.0 46-47 37.82915227658573 40.0 38.0 41.0 33.0 41.0 48-49 37.71425893947968 40.0 38.0 41.0 33.0 41.0 50-51 37.578892461801274 40.0 38.0 41.0 32.0 41.0 52-53 37.444079991220505 40.0 37.5 41.0 32.0 41.0 54-55 37.27217449769142 40.0 37.0 41.0 32.0 41.0 56-57 37.08195305351788 40.0 37.0 41.0 31.0 41.0 58-59 36.89006859639194 40.0 36.0 41.0 31.0 41.0 60-61 36.63680934222565 39.5 36.0 41.0 31.0 41.0 62-63 36.36682575088456 39.0 35.0 41.0 31.0 41.0 64-65 36.06100358584069 39.0 35.0 41.0 30.0 41.0 66-67 35.73763797288935 38.5 35.0 40.5 30.0 41.0 68-69 35.359654400059235 37.0 35.0 40.0 30.0 41.0 70-71 34.96459048430005 37.0 35.0 39.5 29.0 41.0 72-73 34.56796834094047 36.5 35.0 39.0 29.0 41.0 74-75 34.16536649090053 36.0 34.0 39.0 29.0 40.5 76-77 33.74520169031664 35.5 34.0 37.5 28.5 39.5 78-79 33.325261996435316 35.0 34.0 37.0 28.0 39.0 80-81 32.93299984926776 35.0 34.0 37.0 27.0 39.0 82-83 32.77244687349598 35.0 34.0 36.0 28.0 38.5 84-85 32.41290816704306 35.0 34.0 36.0 27.5 37.0 86-87 31.815827348117434 35.0 34.0 36.0 14.0 37.0 88-89 16.419334781596916 18.0 16.5 18.5 2.0 19.0 90-91 2.7459801430105193 2.0 2.0 2.0 2.0 2.0 92-93 2.2892941764466332 2.0 2.0 2.0 2.0 2.0 94-95 2.0997797854842206 2.0 2.0 2.0 2.0 2.0 96-97 2.000040988591949 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17852194144581546 1101 2 0.18392425129147583 1101 3 0.22823507739476767 1101 4 0.27897804405921534 1101 5 0.2308919200113877 1101 6 0.1979860444171564 1101 7 0.2371166240260223 1101 8 0.28650052053757946 1101 9 0.30877549911454594 1101 10-11 0.24186281334879567 1101 12-13 0.3314254235547125 1101 14-15 0.28579925128971695 1101 16-17 0.3409011766596137 1101 18-19 0.2659369201501036 1101 20-21 0.2552319692081184 1101 22-23 0.23615085706184402 1101 24-25 0.34933193429066023 1101 26-27 0.3662719479396017 1101 28-29 0.370490905973746 1101 30-31 0.44315434948286025 1101 32-33 0.3774525474127941 1101 34-35 0.39461153977953245 1101 36-37 0.35046156422075114 1101 38-39 0.3672662572285361 1101 40-41 0.4006033321584539 1101 42-43 0.3953299056991213 1101 44-45 0.29119090216913435 1101 46-47 0.3134672898157831 1101 48-49 0.2412980562610585 1101 50-51 0.28411420184268366 1101 52-53 0.24513928946228702 1101 54-55 0.2113449300061987 1101 56-57 0.1832643555984319 1101 58-59 0.3008510198901888 1101 60-61 0.23985591333971001 1101 62-63 0.21949058646109876 1101 64-65 0.25851683271449133 1101 66-67 0.2119905406221818 1101 68-69 0.31298450958648516 1101 70-71 0.28269475552645673 1101 72-73 0.2614348083583309 1101 74-75 0.20904598451858902 1101 76-77 0.1960352240297496 1101 78-79 0.22125415889154 1101 80-81 0.2756791737449902 1101 82-83 0.17329992196084731 1101 84-85 0.27670642728075734 1101 86-87 0.2050316081432193 1101 88-89 0.7653913637188658 1101 90-91 -0.0015822209795279818 1101 92-93 -0.16642385372097301 1101 94-95 -0.012468688945840523 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.03545386857908639 1102 2 0.07598880865804958 1102 3 0.1615364516357367 1102 4 0.18955650363096765 1102 5 0.23189543551154657 1102 6 0.16803398305570028 1102 7 0.1475640513190939 1102 8 0.1721413003394332 1102 9 0.20126830831876674 1102 10-11 0.1839545359438759 1102 12-13 0.15575907647897225 1102 14-15 0.21379861210786544 1102 16-17 0.20196541443525717 1102 18-19 0.1434281313996948 1102 20-21 0.2356474374088222 1102 22-23 0.21920454833701797 1102 24-25 0.26989601227084137 1102 26-27 0.2965052493138458 1102 28-29 0.19776958925913846 1102 30-31 0.1327996035576433 1102 32-33 0.25366539496791773 1102 34-35 0.15906184339090856 1102 36-37 0.2803768726259932 1102 38-39 0.2237156020671378 1102 40-41 0.19726200905202518 1102 42-43 0.21005186159556644 1102 44-45 0.15157760718670943 1102 46-47 0.14772104500912775 1102 48-49 0.10776977246431585 1102 50-51 0.1707680848724067 1102 52-53 0.14295488549936408 1102 54-55 0.16520878427274255 1102 56-57 0.21978560839485084 1102 58-59 0.3312712819547414 1102 60-61 0.24081788201980459 1102 62-63 0.15625313040484912 1102 64-65 0.18015953006188568 1102 66-67 0.23226059495263485 1102 68-69 0.16536386401281788 1102 70-71 0.20529622564470884 1102 72-73 0.21038495217423758 1102 74-75 0.29673534214082764 1102 76-77 0.21442128986547715 1102 78-79 0.12946125381004947 1102 80-81 0.059210653450961104 1102 82-83 0.24719413736512763 1102 84-85 0.16999022402093544 1102 86-87 0.26815401146696516 1102 88-89 1.071909420606051 1102 90-91 0.15681503053852897 1102 92-93 -0.006387100764757125 1102 94-95 0.11284930402315929 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.12153681788783999 1103 2 0.15880521151652616 1103 3 0.13211015096119283 1103 4 0.19320306744583604 1103 5 0.14726443304043357 1103 6 0.052168091116875814 1103 7 0.0662356602151064 1103 8 0.1538891130690132 1103 9 0.1577519789258588 1103 10-11 0.2567499009341887 1103 12-13 0.19315438803977258 1103 14-15 0.23127874301015794 1103 16-17 0.2793052928831301 1103 18-19 0.3038141595837587 1103 20-21 0.2644232338424004 1103 22-23 0.26079107074401975 1103 24-25 0.30850116821821416 1103 26-27 0.3746024910155725 1103 28-29 0.298415084483878 1103 30-31 0.2102022616755903 1103 32-33 0.23808468301974273 1103 34-35 0.25361837848741686 1103 36-37 0.34679075094028633 1103 38-39 0.28661482066060984 1103 40-41 0.3258725079500451 1103 42-43 0.31892750441932094 1103 44-45 0.28858755843228323 1103 46-47 0.30022488525490587 1103 48-49 0.09250696607949038 1103 50-51 0.12304351397813207 1103 52-53 0.1596813264263659 1103 54-55 0.2917245125837056 1103 56-57 0.2999515737992411 1103 58-59 0.2646617180730857 1103 60-61 0.23623496610449024 1103 62-63 0.2239710226108187 1103 64-65 0.28085144217034497 1103 66-67 0.28966559061149155 1103 68-69 0.19471569731275906 1103 70-71 0.1992960920709379 1103 72-73 0.2570820068727002 1103 74-75 0.3723173898266623 1103 76-77 0.34671875864260926 1103 78-79 0.21990004363172488 1103 80-81 0.2101463376825805 1103 82-83 0.2549110277678537 1103 84-85 0.27630525774830517 1103 86-87 0.36600547743905665 1103 88-89 1.4158608665420687 1103 90-91 -0.4360103885491622 1103 92-93 -0.3037680531457094 1103 94-95 -0.1076268864530312 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.17663151729940552 1104 2 -0.09417539495442995 1104 3 0.0013394073007617635 1104 4 0.07369443466544823 1104 5 -0.0032112968365609618 1104 6 -0.10648161628748909 1104 7 -0.10690228694035397 1104 8 -0.06439400343725055 1104 9 -0.05733596097515914 1104 10-11 0.005112171411852273 1104 12-13 0.037039864656307486 1104 14-15 0.0718445891193582 1104 16-17 0.09219529949273664 1104 18-19 0.04773026604666342 1104 20-21 0.048619522794702164 1104 22-23 -0.1406066175086309 1104 24-25 -0.051097419584664294 1104 26-27 -0.07625337137580601 1104 28-29 -0.057296092185850966 1104 30-31 -0.05437609430770607 1104 32-33 -0.09039043426032123 1104 34-35 -0.12377066892436517 1104 36-37 -0.17513215857269415 1104 38-39 -0.12871461139754814 1104 40-41 -0.06972485465897194 1104 42-43 -0.18414354070328187 1104 44-45 -0.2677819987246153 1104 46-47 -0.224528544481835 1104 48-49 -0.18548145906277824 1104 50-51 -0.2772450514165925 1104 52-53 -0.23115841499324574 1104 54-55 -0.2913100991426987 1104 56-57 -0.19130634562814208 1104 58-59 -0.22909817617333772 1104 60-61 -0.17593088645310218 1104 62-63 -0.24315573659022505 1104 64-65 -0.2451278262599601 1104 66-67 -0.18727142475179903 1104 68-69 -0.16398753171788627 1104 70-71 -0.1586775017772979 1104 72-73 -0.06886792138897846 1104 74-75 -0.1392416693534244 1104 76-77 -0.20011072983895417 1104 78-79 -0.0437407166333017 1104 80-81 -0.17333450254247396 1104 82-83 -0.015983202536354213 1104 84-85 0.050244739620275425 1104 86-87 0.026495554980922265 1104 88-89 0.584988238340042 1104 90-91 -0.5852128840755926 1104 92-93 -0.3037680531457094 1104 94-95 -0.1076268864530312 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.022438189111966267 1105 2 -0.17767061399634798 1105 3 -0.09819945734640356 1105 4 0.038102262729488245 1105 5 0.027115257565540674 1105 6 -0.08301737764390538 1105 7 -0.026611806324105203 1105 8 -0.16180918803316047 1105 9 -0.04757787062791152 1105 10-11 -0.19054469179656053 1105 12-13 -0.14490528428589755 1105 14-15 -0.22450350453596002 1105 16-17 -0.0979751977164014 1105 18-19 -0.16068159472008148 1105 20-21 -0.054274466482929995 1105 22-23 -0.27894039716973396 1105 24-25 -0.14735572259395724 1105 26-27 -0.14126694499100978 1105 28-29 -0.22505568429478018 1105 30-31 -0.2217034310961381 1105 32-33 -0.21186289583675233 1105 34-35 -0.026418915381313468 1105 36-37 -0.23626488595044748 1105 38-39 -0.13009087621170323 1105 40-41 -0.04982178654935865 1105 42-43 -0.11926060985258147 1105 44-45 -0.23117272455858995 1105 46-47 -0.12838198094313924 1105 48-49 -0.1699193223604567 1105 50-51 -0.2633884118971537 1105 52-53 -0.16739371899497257 1105 54-55 -0.211067401852695 1105 56-57 -0.057254897163247165 1105 58-59 -0.23581273118454504 1105 60-61 -0.2865562179171093 1105 62-63 -0.22183166587090142 1105 64-65 -0.18802381088527653 1105 66-67 -0.22648714809158577 1105 68-69 -0.08464799180305249 1105 70-71 -0.16340920627681044 1105 72-73 -0.18105634522183323 1105 74-75 -0.2545803271145388 1105 76-77 -0.25965537049258813 1105 78-79 -0.3642318872197734 1105 80-81 -0.44596935916623437 1105 82-83 -0.3824392024943464 1105 84-85 -0.3700081444447747 1105 86-87 -0.2612812601291097 1105 88-89 0.5894256815645242 1105 90-91 -0.7419944932709948 1105 92-93 -0.3037680531457094 1105 94-95 -0.1076268864530312 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.03401302574836862 1106 2 7.222558217740982E-4 1106 3 0.09990361789134639 1106 4 0.011070447854073961 1106 5 0.06819594774831472 1106 6 0.04499218200592736 1106 7 -0.12924174138311884 1106 8 0.015087610337538138 1106 9 0.09811457404949664 1106 10-11 -0.075568324993327 1106 12-13 0.05245925987237854 1106 14-15 0.01374991799542613 1106 16-17 0.06709600319134523 1106 18-19 0.05336046555513718 1106 20-21 0.022099091111471125 1106 22-23 0.03341911862728608 1106 24-25 0.004565904131965226 1106 26-27 -0.036141939707846404 1106 28-29 0.10036017928000973 1106 30-31 0.11044141725523815 1106 32-33 0.21354081392700408 1106 34-35 0.21780433374694042 1106 36-37 0.2124653441367741 1106 38-39 0.09590873430011726 1106 40-41 0.1675070605919018 1106 42-43 -0.1351517683291732 1106 44-45 0.1270060521370766 1106 46-47 -0.03095219852466613 1106 48-49 0.013010397266697282 1106 50-51 -0.055911229615674074 1106 52-53 -0.004073192664172609 1106 54-55 0.013872541940479266 1106 56-57 -0.07283280683312654 1106 58-59 0.0016725340890673124 1106 60-61 0.04697610626567439 1106 62-63 0.07869921906608113 1106 64-65 0.10547161505524372 1106 66-67 0.029833794219065624 1106 68-69 0.10783429973027125 1106 70-71 0.1327584402782307 1106 72-73 0.18966118059389458 1106 74-75 0.16139669672249823 1106 76-77 0.2458568175958291 1106 78-79 0.2876753814265669 1106 80-81 0.27673800436476625 1106 82-83 0.21729120939446744 1106 84-85 0.2274906983142344 1106 86-87 0.3783500526988668 1106 88-89 1.0326559582649395 1106 90-91 -0.2539466845060545 1106 92-93 -0.2911518512864797 1106 94-95 -0.1076268864530312 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.0884971229581133 1107 2 -0.23684559831428942 1107 3 -0.2251504247063103 1107 4 -0.0436884123725676 1107 5 -0.11933861779147037 1107 6 -0.06209611672957038 1107 7 -0.0755848687263807 1107 8 -0.09373087346376963 1107 9 -0.09259679209805682 1107 10-11 -0.037690657942697214 1107 12-13 -0.11575412296526366 1107 14-15 -0.12191314739699521 1107 16-17 -0.10221672526412107 1107 18-19 -0.0872034209559942 1107 20-21 -0.19694192724950454 1107 22-23 -0.045690613971942184 1107 24-25 -0.03097363532294395 1107 26-27 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0.08048062597455541 2216 4 0.08784276962179405 2216 5 0.06756799852661999 2216 6 0.13628266413566337 2216 7 0.14942571126184845 2216 8 0.08265369646370857 2216 9 0.010771773716513167 2216 10-11 0.00501087882859963 2216 12-13 0.041497154476275 2216 14-15 0.08211384261716148 2216 16-17 0.006433191408468986 2216 18-19 0.051354409803892054 2216 20-21 0.08849975284621792 2216 22-23 0.0225639152554038 2216 24-25 -0.06934073818604247 2216 26-27 -0.02370957086477432 2216 28-29 -0.019526193644892942 2216 30-31 -0.009483421165640493 2216 32-33 -0.03783248441555287 2216 34-35 -0.10193208785284469 2216 36-37 -0.08745165052837933 2216 38-39 -0.10044632338370008 2216 40-41 -0.08078547206930864 2216 42-43 -0.022812005167644145 2216 44-45 -0.01544594372532515 2216 46-47 0.07964684067135153 2216 48-49 0.06009520951820946 2216 50-51 0.056661329932921944 2216 52-53 0.05653424483179492 2216 54-55 -0.010411366756535756 2216 56-57 -0.09940941936281433 2216 58-59 -0.05066434095481043 2216 60-61 -0.06539362792813108 2216 62-63 -0.15839015775762988 2216 64-65 -0.1325824443527921 2216 66-67 -0.07896401799173702 2216 68-69 -0.09337911036737978 2216 70-71 -0.09937706148332381 2216 72-73 -0.05506399786196425 2216 74-75 -0.06573388305830719 2216 76-77 -0.028624467177387203 2216 78-79 0.007480287636468574 2216 80-81 0.16438013036555787 2216 82-83 0.17754705622999012 2216 84-85 0.16528814478228782 2216 86-87 0.08233490178109193 2216 88-89 -0.8451384398125548 2216 90-91 -0.7419944932709948 2216 92-93 -0.3037680531457094 2216 94-95 -0.1076268864530312 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18595.0 3 3154.0 4 2268.0 5 1933.0 6 1995.0 7 2129.0 8 2305.0 9 2701.0 10 3231.0 11 3996.0 12 4691.0 13 5434.0 14 5962.0 15 6308.0 16 6829.0 17 8004.0 18 10019.0 19 12521.0 20 14532.0 21 20040.0 22 23876.0 23 31201.0 24 47504.0 25 80562.0 26 169330.0 27 478793.0 28 526408.0 29 17697.0 30 598.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15212849791355 18.830357473410302 14.079647445220731 23.937866583455417 2 14.371706011619697 19.688960230172604 41.79567157035153 24.143662187856172 3 19.588544594728663 24.416746585535236 28.08618009854823 27.90852872118787 4 12.255837380800092 16.301738729544116 37.85816194849889 33.5842619411569 5 13.780741093516424 38.01451230966649 34.128666111044964 14.076080485772117 6 32.0891091988978 36.13507988808792 18.249773901637955 13.526037011376319 7 29.535652141720043 31.464165392935158 20.801049307953903 18.199133157390904 8 25.832398969731905 36.1490953421093 19.986037434484363 18.03246825367443 9 26.12288908751461 17.558983906027702 20.350307017775826 35.96781998868186 10-11 24.891891659609296 26.712759395558518 27.731896604727087 20.663452340105103 12-13 25.944886548824563 23.805541359880312 27.763655560102578 22.485916531192547 14-15 22.15613358323169 26.309999570280606 25.37770681898566 26.15616002750204 16-17 22.2650032741388 28.91591793398371 26.70824597062762 22.110832821249872 18-19 22.475371900826445 27.936396694214878 27.755966942148756 21.832264462809917 20-21 23.272927772817532 27.222467020785324 27.23423498171834 22.270370224678803 22-23 22.61290204220466 27.646666629203715 27.154469556007825 22.585961772583797 24-25 22.70658911448775 27.448076709488728 27.528335016950766 22.316999159072758 26-27 22.00261004775832 28.000860760430935 27.451646683626247 22.544882508184497 28-29 22.067852110127042 28.140117817120668 26.964888567587497 22.82714150516479 30-31 22.485845713651052 27.541986862495172 27.16284238696404 22.80932503688973 32-33 22.04105851027785 27.825115405783755 27.293215809228826 22.840610274709565 34-35 22.575462932203106 27.98494129531437 27.253954827191478 22.185640945291045 36-37 22.502807227358478 27.339898791065924 27.35196400137907 22.80532998019653 38-39 22.328204911226642 27.89581096590278 27.061593953786023 22.714390169084552 40-41 23.080576960709127 27.320648465968887 27.39052740417925 22.20824716914273 42-43 22.26407759801562 27.82629563616939 27.20822733595308 22.701399429861908 44-45 22.212555359500413 27.848530985784205 27.231948951913015 22.706964702802367 46-47 22.832000983726207 27.59057817714476 26.935917642018858 22.641503197110172 48-49 22.487219958846037 27.668553578659438 26.994752456428863 22.849474006065666 50-51 22.232465707554702 28.00326851289605 27.193379410285967 22.570886369263285 52-53 22.520355463647086 27.696884073684263 27.275693236088998 22.507067226579647 54-55 22.26246584507531 27.54190981975984 27.602732526198388 22.592891808966456 56-57 22.44538600675915 27.670770373974623 26.971088498336655 22.912755120929567 58-59 22.62365332642257 27.585553769099363 26.897176811563543 22.893616092914527 60-61 22.234691422013253 27.525095595974125 27.696057690782062 22.54415529123056 62-63 22.333956536225983 27.462951601728395 27.658771294234626 22.544320567810995 64-65 22.296280845991596 27.761880001890766 27.22301206089717 22.718827091220472 66-67 22.39038196078846 28.036329114593528 26.805415254102826 22.767873670515186 68-69 22.730743549229476 27.309187430996758 27.546690819180093 22.413378200593677 70-71 22.85864356849326 27.21711921598079 27.236390465260186 22.687846750265763 72-73 22.60772066406808 27.556828699418755 27.26405776482597 22.571392871687195 74-75 22.316338052750993 28.062971699360578 27.300352501890764 22.320337745997662 76-77 22.499762001724164 27.371256154899854 27.48913141207022 22.639850431305764 78-79 22.496736602053204 27.519840699241477 27.27268520217201 22.710737496533312 80-81 22.70658911448775 27.723989432876557 27.050255980367787 22.519165472267915 82-83 22.599745935076925 27.74884132048971 27.426946417952337 22.22446632648103 84-85 22.553458745285482 27.76165779130843 27.369092597918176 22.315790865487912 86-87 22.275816474943806 28.02832870554013 27.012660319978842 22.683194499537223 88-89 31.480719291111082 17.61885630763951 33.050672190476426 17.849752210772984 90-91 30.765201676282043 13.86865536439403 15.521483810066213 39.844659149257716 92-93 23.11103408792676 16.661978833881292 33.5005265506668 26.72646052752515 94-95 14.71550779939141 6.409217261368891 26.040402384830593 52.83487255440911 96-97 25.26418647598325 6.762840810639305 14.26290004168585 53.71007267169159 98-99 57.397990645782535 14.030377489161062 18.791446488373637 9.780185376682763 100-101 30.955370758022116 6.705317468559217 12.069995026316796 50.269316747101875 102-103 22.700296317598333 1.8526898363909587 36.53643917031072 38.91057467569998 104-105 41.74924386353543 4.370219128478818 39.14855486620786 14.731982141777886 106-107 4.807313222451651 1.3957122308892917 5.958375263507517 87.83859928315154 108-109 17.88531384072977 9.662921688422301 19.261823205193352 53.18994126565458 110-111 39.801356918147604 2.505881988647259 23.131494158211506 34.56126693499363 112-113 27.04897768895934 2.6679732341884557 37.57529347843303 32.707755598419176 114-115 26.53540037719048 3.609072581653735 22.62967798738363 47.22584905377216 116-117 18.147373149855774 3.769545719645025 19.208299837644425 58.874781292854784 118-119 18.602478173247032 3.924184640202962 14.586730787943731 62.88660639860628 120-121 24.16948437961128 3.972174206332476 28.741515240946075 43.11682617311016 122-123 33.141321464502866 1.4592264117930098 32.765955120073485 32.63349700363064 124-125 37.68135073253123 1.6158786711539717 18.186720384083046 42.516050212231754 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7.0 1 20.0 2 57.0 3 94.0 4 127.0 5 154.5 6 160.5 7 154.0 8 142.0 9 138.0 10 146.0 11 141.0 12 128.5 13 132.0 14 147.5 15 171.0 16 212.5 17 281.5 18 400.0 19 566.5 20 832.0 21 1273.0 22 1872.0 23 2849.0 24 4210.0 25 5899.0 26 8052.5 27 10479.0 28 13219.5 29 16502.0 30 20379.0 31 24732.0 32 29438.5 33 34366.0 34 39181.0 35 43585.0 36 47167.5 37 49619.0 38 51702.5 39 53414.5 40 54931.0 41 56234.5 42 57353.5 43 58757.5 44 60135.5 45 60662.0 46 59754.5 47 58881.0 48 57822.5 49 56079.5 50 54758.5 51 53261.5 52 50274.5 53 46148.5 54 41990.0 55 37870.5 56 33622.5 57 29323.0 58 25173.5 59 21237.0 60 17524.5 61 14158.0 62 11172.0 63 8701.0 64 6614.5 65 4891.0 66 3610.5 67 2618.0 68 1901.5 69 1445.5 70 1027.5 71 683.5 72 463.0 73 330.5 74 249.0 75 206.5 76 168.0 77 105.0 78 68.0 79 45.0 80 31.0 81 22.0 82 15.5 83 17.5 84 18.5 85 17.0 86 13.0 87 7.0 88 3.5 89 5.5 90 8.0 91 8.0 92 7.0 93 3.5 94 4.0 95 6.0 96 5.5 97 9.0 98 8.5 99 2.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 7.933275861157095E-4 3 0.044029681029421876 4 0.050574633614876484 5 0.052624063212342066 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.594382182920186E-4 12-13 0.0017849870687603462 14-15 6.611063217630912E-5 16-17 2.9749784479339103E-4 18-19 0.007668833332451859 20-21 0.0022808168100826648 22-23 6.941616378512458E-4 24-25 0.0 26-27 0.0 28-29 0.0011569360630854096 30-31 0.0 32-33 0.0012230466952617188 34-35 1.6527658044077282E-4 36-37 1.6527658044077282E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.9833189652892737E-4 46-47 0.0 48-49 2.3138721261708193E-4 50-51 9.916594826446369E-5 52-53 0.0 54-55 0.0015205445400551098 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.305531608815456E-5 66-67 0.0 68-69 3.9666379305785475E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.636084769697002E-4 80-81 0.0 82-83 6.941616378512458E-4 84-85 6.611063217630912E-5 86-87 0.001057770114820946 88-89 19.28576056315681 90-91 26.288793718961056 92-93 40.29899194508057 94-95 44.74033727000111 96-97 48.3781409161347 98-99 93.03147659419179 100-101 51.616801620503814 102-103 53.102836410562894 104-105 74.84140059340903 106-107 81.63820163213929 108-109 12.63754317024281 110-111 39.15213775340206 112-113 51.99868968727026 114-115 86.78455073858798 116-117 66.44700307282218 118-119 71.80490620223506 120-121 80.39902394262654 122-123 75.81831740507835 124-125 49.91825420331399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1512616.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.02382895929455 #Duplication Level Percentage of deduplicated Percentage of total 1 78.65503255240507 49.57141318370522 2 12.981500367655045 16.362877176122215 3 3.9855123239016557 7.535467410502153 4 1.6474625531358276 4.1531759266270045 5 0.7963838674046178 2.5095580322625066 6 0.4753538392114031 1.7975171434562083 7 0.2872235884361706 1.2671351217473217 8 0.19162481301665665 0.9661543551934854 9 0.14608605764559762 0.828621243935441 >10 0.7589762825947314 8.685694721180372 >50 0.048497810757219435 2.1176754242597764 >100 0.02330215327729742 2.6930131880695662 >500 0.0026239573781792714 1.155618424899959 >1k 4.198331805086834E-4 0.35607864803872236 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2056 0.13592345975449158 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.611063217630912E-5 7 0.0 0.0 0.0 0.0 6.611063217630912E-5 8 0.0 0.0 0.0 0.0 6.611063217630912E-5 9 0.0 0.0 0.0 1.3222126435261824E-4 6.611063217630912E-5 10-11 0.0 0.0 0.0 1.3222126435261824E-4 1.3222126435261824E-4 12-13 0.0 6.611063217630912E-5 0.0 1.9833189652892737E-4 4.6277442523416385E-4 14-15 0.0 6.611063217630912E-5 0.0 1.9833189652892737E-4 4.6277442523416385E-4 16-17 0.0 6.611063217630912E-5 0.0 2.644425287052365E-4 4.6277442523416385E-4 18-19 0.0 6.611063217630912E-5 0.0 2.644425287052365E-4 4.6277442523416385E-4 20-21 0.0 6.611063217630912E-5 0.0 2.644425287052365E-4 5.28885057410473E-4 22-23 0.0 6.611063217630912E-5 0.0 2.644425287052365E-4 5.949956895867821E-4 24-25 0.0 6.611063217630912E-5 0.0 2.9749784479339103E-4 5.949956895867821E-4 26-27 0.0 6.611063217630912E-5 0.0 5.949956895867821E-4 7.933275861157095E-4 28-29 0.0 6.611063217630912E-5 0.0 0.001355267959614337 7.933275861157095E-4 30-31 0.0 6.611063217630912E-5 0.0 0.0039005272984022382 7.933275861157095E-4 32-33 0.0 6.611063217630912E-5 0.0 0.009453820401212204 7.933275861157095E-4 34-35 0.0 6.611063217630912E-5 0.0 0.01725487499801668 7.933275861157095E-4 36-37 0.0 6.611063217630912E-5 0.0 0.029815895111515417 7.933275861157095E-4 38-39 0.0 6.611063217630912E-5 0.0 0.05044241235052386 7.933275861157095E-4 40-41 0.0 6.611063217630912E-5 0.0 0.07586195042231472 7.933275861157095E-4 42-43 0.0 6.611063217630912E-5 0.0 0.10190953949978052 7.933275861157095E-4 44-45 0.0 6.611063217630912E-5 0.0 0.13202293245608931 8.26382902203864E-4 46-47 0.0 6.611063217630912E-5 0.0 0.16858211204958826 8.594382182920186E-4 48-49 0.0 6.611063217630912E-5 0.0 0.20927320615410655 9.255488504683277E-4 50-51 0.0 6.611063217630912E-5 0.0 0.2552862061488177 9.91659482644637E-4 52-53 0.0 6.611063217630912E-5 0.0 0.300902542350471 0.0011238807469972551 54-55 0.0 9.916594826446369E-5 0.0 0.3492624697874411 0.0011238807469972551 56-57 0.0 1.3222126435261824E-4 0.0 0.4077042686312984 0.0011238807469972551 58-59 0.0 1.3222126435261824E-4 0.0 0.47444295181328244 0.0011238807469972551 60-61 0.0 1.3222126435261824E-4 0.0 0.5444871666040819 0.0011569360630854096 62-63 0.0 1.3222126435261824E-4 0.0 0.6172749726301983 0.0011899913791735641 64-65 0.0 1.3222126435261824E-4 0.0 0.695054131385626 0.0012561020113498733 66-67 0.0 1.3222126435261824E-4 0.0 0.7802376809448003 0.0012561020113498733 68-69 0.0 1.3222126435261824E-4 0.0 0.8860146924268948 0.0012561020113498733 70-71 0.0 1.3222126435261824E-4 0.0 0.9994274819253532 0.0012561020113498733 72-73 0.0 1.3222126435261824E-4 0.0 1.1140963734351614 0.001421378591790646 74-75 0.0 1.3222126435261824E-4 0.0 1.2276413841979723 0.001586655172231419 76-77 0.0 1.3222126435261824E-4 0.0 1.3541440788673398 0.001586655172231419 78-79 0.0 1.6527658044077282E-4 0.0 1.3888852160759901 0.001652765804407728 80-81 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.001652765804407728 82-83 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.001652765804407728 84-85 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 86-87 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 88-89 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 90-91 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 92-93 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 94-95 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 96-97 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 98-99 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 100-101 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 102-103 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 104-105 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 106-107 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 108-109 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 110-111 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 112-113 0.0 1.9833189652892737E-4 0.0 1.3895132670816652 0.0017188764365840372 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTCG 45 7.625204E-9 1635.7942 108-109 CCGCTCC 630 0.0 1480.0043 108-109 CCGCGCC 635 0.0 1468.3506 108-109 CCGCTCG 210 0.0 1402.1094 108-109 CGGTACG 25 0.005519807 1216.4779 110-111 CGTCGCG 85 6.730261E-11 1154.6782 108-109 CGTGTCG 80 3.216337E-8 1140.448 110-111 CGTCGCC 270 0.0 999.652 108-109 CTCCGCC 395 0.0 846.91504 110-111 CGTCGCT 85 5.9077815E-5 715.5752 110-111 CCGCGCG 250 0.0 687.03357 108-109 TTGTTCG 235 0.0 626.4744 108-109 CGACGCC 80 9.3875344E-5 613.42285 108-109 CGGCGCC 360 0.0 613.4228 108-109 CCTCTCG 345 0.0 568.97186 108-109 CGTCTCT 385 0.0 552.9445 110-111 CCGCGCT 90 1.3362174E-4 545.2647 108-109 CGGGGCG 110 2.4381683E-4 446.1257 108-109 CTGTTCG 280 4.9112714E-11 438.15918 108-109 CCGCACC 355 0.0 414.7084 108-109 >>END_MODULE