FastQCFastQC Report
Fri 27 May 2016
SRR939929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512616
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37150.2456009985349884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.16137605314237058No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21760.14385673561564866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.11291695975713598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG751.4721463E-647.5952268
CCGTCGT1102.0415937E-532.451299
GGACCGA3800.031.312656
GTATCAA77800.031.249541
GTACATG63200.030.0771471
TACATGG62750.029.527282
ATACCGT1852.8734576E-828.9430436
GTCCTAC18450.028.4216521
ACATGGG63700.028.058123
TACCGTC2154.0399755E-927.6716447
ATCAACG89300.026.886263
TCAACGC90350.026.5078624
CAACGCA91200.026.2608015
TATCAAC92650.026.0427232
AACGCAG93700.025.588156
GGTATCA57100.025.3590641
TAAGGTG8450.024.6767585
GTAAGGT7800.024.4417444
AGAGTAC78800.024.23551410-11
GTAGGAC19200.023.5824623
Produced by FastQC (version 0.11.5)