FastQCFastQC Report
Fri 27 May 2016
SRR939928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688715
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16860.24480372868312728No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14520.21082741046731956No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA9380.1361956687454172No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9000.13067814698387575No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA8320.12080468698953849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG153.378318E-73992.873110-111
CAGCGCG155.9723607E-73305.0251108-109
CGGCGTT109.086373E-42994.6548110-111
CGGTAGT109.086373E-42994.6548110-111
CCGCTCG256.87578E-102974.5227108-109
CCGCTCC1650.02704.1116108-109
CGTCGCG301.4260877E-92478.7688108-109
CCGCGCT100.00132731342478.7688108-109
CGTCGGC150.00204420881996.4365110-111
CGTCGCT150.00204420881996.4365110-111
CGACGCC252.7641563E-61983.0151108-109
GTTGTCG354.28959E-61711.2313110-111
CGGCGCG150.00298605671652.5126108-109
ACGCTCG150.00298605671652.5126108-109
CGTCGAC150.00298605671652.5126108-109
CCGCGTG151.3561643E-41652.5126108-109
CGCGTCT150.00298605671652.5126108-109
CCGCACC457.219569E-91652.5126108-109
CGTCGTG200.00363374851497.3274110-111
CGCGTGT200.00363374851497.3274110-111
Produced by FastQC (version 0.11.5)