##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939928_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688715 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.866971098349826 34.0 31.0 34.0 30.0 34.0 2 31.949947365746354 34.0 31.0 34.0 30.0 34.0 3 32.03731732284036 34.0 31.0 34.0 30.0 34.0 4 35.26886302752227 37.0 35.0 37.0 33.0 37.0 5 35.28569872879203 37.0 35.0 37.0 33.0 37.0 6 35.36601642188714 37.0 37.0 37.0 33.0 37.0 7 35.28894535475487 37.0 37.0 37.0 33.0 37.0 8 35.32032843774275 37.0 37.0 37.0 33.0 37.0 9 36.999223191015155 39.0 38.0 39.0 34.0 39.0 10-11 36.98175733068105 39.0 38.0 39.0 34.0 39.0 12-13 36.96002482884793 39.0 38.0 39.0 34.0 39.0 14-15 38.492211582439765 41.0 39.5 41.0 34.5 41.0 16-17 38.493076236178965 41.0 39.0 41.0 34.0 41.0 18-19 38.497350137575054 41.0 39.5 41.0 34.0 41.0 20-21 38.53793877002824 41.0 39.5 41.0 34.0 41.0 22-23 38.52193868290948 41.0 39.0 41.0 34.0 41.0 24-25 38.54069099700166 41.0 39.0 41.0 34.0 41.0 26-27 38.50621084193026 41.0 39.0 41.0 34.0 41.0 28-29 38.44375830350726 41.0 39.0 41.0 34.0 41.0 30-31 38.35454288058195 41.0 39.0 41.0 34.0 41.0 32-33 38.26036096208156 41.0 39.0 41.0 33.5 41.0 34-35 38.221577866025854 40.5 39.0 41.0 33.0 41.0 36-37 38.382245921752826 41.0 39.0 41.0 34.0 41.0 38-39 38.33055981066188 41.0 39.0 41.0 34.0 41.0 40-41 38.23440755610085 41.0 39.0 41.0 33.0 41.0 42-43 38.20457228316502 41.0 39.0 41.0 33.0 41.0 44-45 38.14710584203916 41.0 39.0 41.0 33.0 41.0 46-47 38.0388767487277 41.0 38.5 41.0 33.0 41.0 48-49 37.95621701284276 41.0 38.0 41.0 33.0 41.0 50-51 37.86038637172125 41.0 38.0 41.0 33.0 41.0 52-53 37.77108963794893 40.0 38.0 41.0 32.5 41.0 54-55 37.647748342928494 40.0 38.0 41.0 32.0 41.0 56-57 37.46394517325744 40.0 38.0 41.0 31.5 41.0 58-59 37.324811424174 40.0 38.0 41.0 31.0 41.0 60-61 37.13487944940941 40.0 37.0 41.0 31.0 41.0 62-63 36.880836049744815 40.0 37.0 41.0 31.0 41.0 64-65 36.60682575521079 40.0 36.0 41.0 30.5 41.0 66-67 36.30784286678815 39.0 36.0 41.0 30.0 41.0 68-69 35.897304400223604 39.0 35.0 41.0 29.5 41.0 70-71 35.47736727093209 38.5 35.0 40.5 29.0 41.0 72-73 35.078615973225496 37.5 35.0 40.0 29.0 41.0 74-75 34.63897838728647 37.0 35.0 39.0 28.0 41.0 76-77 34.142156770217 36.5 34.0 39.0 28.0 41.0 78-79 33.65446665166288 36.0 34.0 39.0 26.5 40.0 80-81 33.192222472285344 35.5 34.0 37.5 26.0 39.0 82-83 32.942569858359406 35.0 34.0 37.0 26.0 39.0 84-85 32.4556260572225 35.0 34.0 37.0 25.5 39.0 86-87 31.777401392448255 35.0 34.0 36.0 12.0 37.0 88-89 16.194478848289933 18.0 16.5 18.5 2.0 19.5 90-91 2.7764024306135338 2.0 2.0 2.0 2.0 2.0 92-93 2.301312589387482 2.0 2.0 2.0 2.0 2.0 94-95 2.1011535976419857 2.0 2.0 2.0 2.0 2.0 96-97 2.0000341215161566 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1365394688154744 1101 2 0.16740545991143208 1101 3 0.20003799012843615 1101 4 0.14057497662604135 1101 5 0.15925573855471953 1101 6 0.1048701678965358 1101 7 0.11247716382218442 1101 8 0.23377214586986383 1101 9 0.3326440603230836 1101 10-11 0.17705996918684974 1101 12-13 0.2363274234136412 1101 14-15 0.16306481614631707 1101 16-17 0.3252026293045347 1101 18-19 0.38241955705813524 1101 20-21 0.3370207647050165 1101 22-23 0.2742536148567183 1101 24-25 0.24373317152205232 1101 26-27 0.29542685453174755 1101 28-29 0.3557786638899145 1101 30-31 0.31372610365101394 1101 32-33 0.354884608659205 1101 34-35 0.3447501500282897 1101 36-37 0.3132644446729813 1101 38-39 0.2759314844623546 1101 40-41 0.17017268662704055 1101 42-43 0.31355971576844865 1101 44-45 0.22307006604936674 1101 46-47 0.25461658506533524 1101 48-49 0.22901238426324255 1101 50-51 0.20762319280142094 1101 52-53 0.1465466565691642 1101 54-55 0.18918380317640526 1101 56-57 0.2563971831227789 1101 58-59 0.21199909713586607 1101 60-61 0.29980245448970066 1101 62-63 0.4225518564802897 1101 64-65 0.4839721789736444 1101 66-67 0.342673459925912 1101 68-69 0.3144796300438131 1101 70-71 0.5434054602391925 1101 72-73 0.5649502822911998 1101 74-75 0.5608767916386199 1101 76-77 0.3910664583509842 1101 78-79 0.4234666552652371 1101 80-81 0.4603835283235469 1101 82-83 0.5330309086431271 1101 84-85 0.43273869478638716 1101 86-87 0.44296731402912215 1101 88-89 0.6173775396474639 1101 90-91 -0.2364761490291185 1101 92-93 -0.2525760530741654 1101 94-95 -0.04040764555597898 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.07435451777944735 1102 2 0.03032844507315602 1102 3 -0.02031121131677338 1102 4 -0.1520099636861687 1102 5 -0.1334311129681538 1102 6 -0.08774416575433008 1102 7 -0.18520870077107077 1102 8 -0.10810671066489874 1102 9 -0.05670503813816197 1102 10-11 -0.07034612231440462 1102 12-13 -0.1424272001370781 1102 14-15 -0.22474864128268734 1102 16-17 -0.22829002879729643 1102 18-19 -0.1977057186385025 1102 20-21 -0.06074566338213572 1102 22-23 -0.07505993268324573 1102 24-25 -0.1447051625407454 1102 26-27 -0.006422351204463439 1102 28-29 -0.08701052794346253 1102 30-31 -0.17169254760238317 1102 32-33 -0.1261818975241198 1102 34-35 -0.1572607190705071 1102 36-37 -0.09902433093355256 1102 38-39 0.006690111514529917 1102 40-41 -0.015926838788544728 1102 42-43 0.032361475935971384 1102 44-45 0.055325286048848454 1102 46-47 -0.22367900893756598 1102 48-49 -0.26168419495287765 1102 50-51 -0.16978352089458326 1102 52-53 -0.20218170607168418 1102 54-55 -0.17718056265695736 1102 56-57 -0.2049645222916041 1102 58-59 -0.2541111343205884 1102 60-61 -0.2572238407481464 1102 62-63 -0.35639575472092844 1102 64-65 -0.4266372071561335 1102 66-67 -0.4345303968233907 1102 68-69 -0.5012423733374973 1102 70-71 -0.34352297878143645 1102 72-73 -0.23787619831777818 1102 74-75 -0.2966089622468786 1102 76-77 -0.29355964550833846 1102 78-79 -0.4767021955195858 1102 80-81 -0.40918245864898495 1102 82-83 -0.4157281346358772 1102 84-85 -0.6261016200012506 1102 86-87 -0.33135191908365513 1102 88-89 0.4673797924215961 1102 90-91 -0.009969364960737082 1102 92-93 -0.04370634494787273 1102 94-95 0.08167715744428694 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.1084717285548642 1103 2 0.10792441947998199 1103 3 0.09668259033612259 1103 4 0.2848402096009508 1103 5 0.07915386437122152 1103 6 0.03711420534671106 1103 7 0.1139615205032527 1103 8 0.007920682030380988 1103 9 0.01615053370934305 1103 10-11 0.043252751319293736 1103 12-13 0.07646859431773123 1103 14-15 0.24094800627796076 1103 16-17 0.1892861175636753 1103 18-19 0.2705694079943868 1103 20-21 0.31250480316011675 1103 22-23 0.3549129078925972 1103 24-25 0.34580153567306127 1103 26-27 0.22606981755053823 1103 28-29 0.30245644752974954 1103 30-31 0.25613086615395275 1103 32-33 0.3529694954377547 1103 34-35 0.3849821828223128 1103 36-37 0.39960103563536364 1103 38-39 0.23749523014955543 1103 40-41 0.18787714681529621 1103 42-43 0.2100724196843089 1103 44-45 0.2745959805303215 1103 46-47 0.33259765061988844 1103 48-49 0.17783609963523617 1103 50-51 0.2697076148025559 1103 52-53 0.20249371381634518 1103 54-55 0.14279661009697975 1103 56-57 0.05441214393073324 1103 58-59 0.09644538394120872 1103 60-61 0.18562056740967847 1103 62-63 0.16113706985918697 1103 64-65 0.24937002424882593 1103 66-67 0.3302406642269915 1103 68-69 0.338683646390578 1103 70-71 0.2145032142337655 1103 72-73 0.23965895897360667 1103 74-75 0.31433645230924157 1103 76-77 0.14281565441168453 1103 78-79 0.20359972396855142 1103 80-81 0.2589810310870817 1103 82-83 0.25640512982693053 1103 84-85 0.21060859060925452 1103 86-87 0.14202184791180983 1103 88-89 0.8609789992997605 1103 90-91 -0.5423232372614573 1103 92-93 -0.3154005598239058 1103 94-95 -0.1047897847044732 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.0222393062582924 1104 2 0.026325090192216294 1104 3 -0.0635278057034796 1104 4 -0.02070115098671721 1104 5 0.08121989758744519 1104 6 0.03354705427676663 1104 7 0.08148387757767495 1104 8 0.04701561974996338 1104 9 0.020995231888839783 1104 10-11 0.09706243672331993 1104 12-13 0.07626398716347182 1104 14-15 0.10812203214829452 1104 16-17 0.16902402078948597 1104 18-19 0.12858202377209693 1104 20-21 0.09132147514989697 1104 22-23 -0.02562146610931393 1104 24-25 0.14751724151783208 1104 26-27 0.22029191113087165 1104 28-29 0.14119706554316025 1104 30-31 0.15053660713127215 1104 32-33 0.2570426749331247 1104 34-35 0.17871321923240657 1104 36-37 0.24564263576311873 1104 38-39 0.2706036619604788 1104 40-41 0.2576411998335004 1104 42-43 0.25971909806182225 1104 44-45 0.1526311529991844 1104 46-47 0.12639156630177695 1104 48-49 0.24154078663619316 1104 50-51 0.13722797570962086 1104 52-53 0.19857723345863576 1104 54-55 0.20361683526465413 1104 56-57 0.2872660644034255 1104 58-59 0.2527612574640443 1104 60-61 0.17436318159045072 1104 62-63 0.29057155564647275 1104 64-65 0.2553415589022947 1104 66-67 0.26808600118003767 1104 68-69 0.3278284882941236 1104 70-71 0.15336659653973328 1104 72-73 0.30556541857118447 1104 74-75 0.21401107702978095 1104 76-77 0.22913591950270984 1104 78-79 0.2626583314422035 1104 80-81 0.18221043063994102 1104 82-83 0.06961676349029489 1104 84-85 0.13234286081046776 1104 86-87 0.08773408042538833 1104 88-89 0.8050413994913939 1104 90-91 -0.6184261197271228 1104 92-93 -0.3154005598239058 1104 94-95 -0.1047897847044732 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.16763673756769037 1105 2 -0.11227087783832346 1105 3 -0.039487088378734825 1105 4 0.04653213860730432 1105 5 -0.17540064398477995 1105 6 -0.10815639317641512 1105 7 -0.010269273641064558 1105 8 -0.19835198930010023 1105 9 -0.07445786763798168 1105 10-11 -0.03732561051513983 1105 12-13 0.0049142317059960305 1105 14-15 -0.013102469819976648 1105 16-17 -0.05306433262416732 1105 18-19 0.08161196532046233 1105 20-21 0.07301458421983398 1105 22-23 -0.09401384751613051 1105 24-25 -0.10210500986393356 1105 26-27 0.02920777259653562 1105 28-29 0.005686707278421466 1105 30-31 -0.08521232920814725 1105 32-33 -0.016538779597873088 1105 34-35 0.08827721780094322 1105 36-37 -0.03887464126579232 1105 38-39 0.0022073042524226594 1105 40-41 -0.05437816475355817 1105 42-43 -0.07631911419314008 1105 44-45 -0.12822295650816073 1105 46-47 0.03239279895516489 1105 48-49 0.16569681669703584 1105 50-51 -0.0038989080630926765 1105 52-53 -0.12971414989657148 1105 54-55 -0.10632690007273027 1105 56-57 -0.2262811939368632 1105 58-59 -0.10656018672856504 1105 60-61 -0.16508669713235946 1105 62-63 -0.13120366404417183 1105 64-65 -0.14564754603230057 1105 66-67 -0.11616188143955242 1105 68-69 0.006864434057376911 1105 70-71 -0.2254784867637838 1105 72-73 -0.24046921501265217 1105 74-75 -0.18802379513213907 1105 76-77 -0.16710548174584972 1105 78-79 -0.2752268727408662 1105 80-81 -0.33317387887534977 1105 82-83 -0.20977207900840966 1105 84-85 -0.11938881838155169 1105 86-87 -0.003188023833235576 1105 88-89 0.22460082531349457 1105 90-91 -0.7847627533904893 1105 92-93 -0.3154005598239058 1105 94-95 -0.1047897847044732 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 0.10746366403873608 1106 2 0.007956880917358689 1106 3 0.008327837668808513 1106 4 0.11320133722524872 1106 5 -0.031397862733683723 1106 6 0.09970122546498317 1106 7 -0.08090292366307494 1106 8 -0.04650600205334143 1106 9 0.04680214817697248 1106 10-11 -0.052767104911737306 1106 12-13 0.02689108188070577 1106 14-15 0.02748130076186328 1106 16-17 -0.14688286572846465 1106 18-19 -0.11227804671604247 1106 20-21 0.03419295434437686 1106 22-23 0.04802375001268189 1106 24-25 -0.005067003327596353 1106 26-27 -0.06973747493091764 1106 28-29 0.14139345278615423 1106 30-31 0.18711169043979226 1106 32-33 0.21313452078113926 1106 34-35 0.05466994681593462 1106 36-37 0.24740675979919047 1106 38-39 0.2114224383094978 1106 40-41 0.1845635380421058 1106 42-43 -0.14591515110509334 1106 44-45 0.17891247172270397 1106 46-47 -0.036135923110592216 1106 48-49 0.11308384297868201 1106 50-51 0.04760000709316614 1106 52-53 0.09403535832058907 1106 54-55 0.06036362536558926 1106 56-57 -0.3060999841315919 1106 58-59 -0.19551591263900292 1106 60-61 0.0471935587115766 1106 62-63 -0.08927114249336654 1106 64-65 -0.021256188166304923 1106 66-67 -0.12224732895219859 1106 68-69 -0.2802992546450582 1106 70-71 -0.21278805481288998 1106 72-73 -0.21065597563418947 1106 74-75 -0.2613622507976672 1106 76-77 -0.1263590819858038 1106 78-79 -0.31896097500833065 1106 80-81 -0.09641793171132917 1106 82-83 -0.19938718346114115 1106 84-85 -0.16825158272700236 1106 86-87 -0.20946512058104005 1106 88-89 0.5266012615881763 1106 90-91 -0.14460756425372878 1106 92-93 -0.2867875627337022 1106 94-95 -0.1047897847044732 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.12360801351642081 1107 2 0.07962777088763673 1107 3 0.1465104630806593 1107 4 0.17761201366307944 1107 5 0.13667672532150021 1107 6 0.11615967451314901 1107 7 -0.04410261269528348 1107 8 -0.09566488761701208 1107 9 0.0752663854999227 1107 10-11 -0.020759345454898437 1107 12-13 0.09347788804073787 1107 14-15 -0.018095375917305034 1107 16-17 -0.10552488709276275 1107 18-19 0.02952040820883184 1107 20-21 -0.01215515456435412 1107 22-23 -0.024359160391568935 1107 24-25 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2216 2 -0.25317214524561393 2216 3 -0.24625572135218832 2216 4 -0.16831751976645393 2216 5 -0.2773213136765378 2216 6 -0.13039469704122553 2216 7 -0.038662065588070504 2216 8 -0.08449395977942231 2216 9 -0.3050816615147909 2216 10-11 -0.25736648831203723 2216 12-13 -0.22636315055029854 2216 14-15 -0.28385430834290304 2216 16-17 -0.2448558493403894 2216 18-19 -0.26603511651336476 2216 20-21 -0.2592772454364507 2216 22-23 -0.27606347459587965 2216 24-25 -0.4666133679716893 2216 26-27 -0.45004690846538153 2216 28-29 -0.3010010828431078 2216 30-31 -0.45179880498136527 2216 32-33 -0.6042663131085391 2216 34-35 -0.44220021139636856 2216 36-37 -0.4894464088556276 2216 38-39 -0.5626723442115633 2216 40-41 -0.5208707585051897 2216 42-43 -0.5474422430722896 2216 44-45 -0.5508583030892922 2216 46-47 -0.5340759983118204 2216 48-49 -0.4288550922374057 2216 50-51 -0.5632678774471316 2216 52-53 -0.43650136368679426 2216 54-55 -0.5638762533299158 2216 56-57 -0.40438158250411504 2216 58-59 -0.4288085959498673 2216 60-61 -0.2492545373242976 2216 62-63 -0.47991995779058527 2216 64-65 -0.5755082225044177 2216 66-67 -0.5597886612862055 2216 68-69 -0.5184723024992479 2216 70-71 -0.6806318925948958 2216 72-73 -0.4555193506200226 2216 74-75 -0.5895177579965818 2216 76-77 -0.7592842618011346 2216 78-79 -0.5976806488843991 2216 80-81 -0.6116986924152172 2216 82-83 -0.6480185714717663 2216 84-85 -0.7408079364121143 2216 86-87 -0.5456745282926327 2216 88-89 -1.3029493102938705 2216 90-91 -0.7847627533904893 2216 92-93 -0.3154005598239058 2216 94-95 -0.1047897847044732 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8820.0 3 1311.0 4 907.0 5 899.0 6 808.0 7 759.0 8 910.0 9 1129.0 10 1274.0 11 1618.0 12 1934.0 13 2216.0 14 2649.0 15 3033.0 16 3363.0 17 3893.0 18 5019.0 19 5982.0 20 6602.0 21 9333.0 22 10723.0 23 13779.0 24 20688.0 25 34336.0 26 61167.0 27 173210.0 28 300715.0 29 11194.0 30 444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54826742556791 20.554220541152727 14.839955569430025 24.05755646384934 2 12.531090689708746 20.10698246434281 46.70101101913575 20.660915826812698 3 19.24784224180255 25.676668903588663 29.238749299146193 25.83673955546259 4 11.182934712346478 17.71721999084854 40.19523107427933 30.904614222525655 5 11.757963837517233 38.92296987331365 35.315582813886834 14.00348347528229 6 28.771698017322116 37.622383714598925 20.592117203778052 13.013801064300909 7 27.67719593736161 31.70977835534292 22.483175188575828 18.129850518719646 8 23.246480764902756 40.99896183472118 20.726134903407072 15.028422496968993 9 25.197215103489835 18.745054195131512 22.442810160951918 33.61492054042674 10-11 23.45286615972784 27.031010194436256 30.90666740234264 18.609456243493263 12-13 25.94170449166398 24.52831284539813 29.952170617600167 19.577812045337726 14-15 19.87668320957611 30.57502824104055 25.979434133762346 23.568854415620997 16-17 19.323592936094524 31.055338766176018 30.70845962575095 18.91260867197851 18-19 20.726493089160254 28.78680402431082 31.36541813902125 19.121284747507676 20-21 21.331581220936773 29.31112216264879 28.997266559458684 20.360030056955754 22-23 20.046042801946257 31.61496833922118 29.17895418516147 19.1600346736711 24-25 20.90509136580443 29.32853212141452 31.142344801550713 18.624031711230334 26-27 18.69045976928047 31.39615080258162 30.378894027282694 19.534495400855214 28-29 18.912832307497965 31.235271312764358 28.283580680899135 21.568315698838546 30-31 20.139535221390563 29.396992950639962 30.069404615842547 20.39406721212693 32-33 19.047269876071756 30.74676385389971 30.912219310154565 19.293746959873967 34-35 19.248628603455135 31.17738277427205 30.719863397578678 18.85412522469414 36-37 18.645548079464046 30.464750244658884 30.278243218520313 20.611458457356754 38-39 20.300124144239636 30.988580181933017 29.089971904198396 19.621323769628944 40-41 22.17092701625491 28.43164443928185 30.78080192822866 18.616626616234583 42-43 19.17019376665239 31.33850721996762 30.78922340881206 18.702075604567927 44-45 18.577186723642505 31.512545864532832 29.783015566716468 20.127251845108194 46-47 20.69898288842264 29.26065208395345 29.663866766369253 20.376498261254657 48-49 19.995673079366323 29.965522573675624 29.46879789287677 20.57000645408128 50-51 18.69812338512313 31.568351716642695 29.631189166467625 20.102335731766548 52-53 18.70541515721307 30.395083597714585 30.71466426606071 20.18483697901164 54-55 19.218488639531643 29.54549414552551 30.821804432673545 20.414212782269306 56-57 19.756430453816165 30.195581626652533 28.351712972710047 21.696274946821255 58-59 19.868378066399018 30.95649143695142 27.95662937499546 21.218501121654096 60-61 19.334557835969886 28.558329642885667 31.51543091118968 20.59168160995477 62-63 20.11906231169642 28.656628649005754 30.780584131317017 20.443724907980805 64-65 20.676041613729918 29.94388099576748 28.85286366639321 20.52721372410939 66-67 20.357767726853634 31.63942995288327 28.044691926268484 19.958110393994613 68-69 21.200848539231874 28.91944830430092 30.62030192547549 19.259401230991717 70-71 22.108274104673196 28.82701843287862 29.534495400855214 19.530212061592966 72-73 20.76003862265233 30.634442403606716 28.773803387467968 19.831715586272985 74-75 19.967911255018404 30.895798697574467 30.49781114103802 18.63847890636911 76-77 20.433851447986466 29.28453714526328 30.877068163173448 19.404543243576803 78-79 19.82557282693842 29.274013865021857 30.12937928290728 20.77103402513244 80-81 20.304770478354616 30.313627552761304 29.75831802705038 19.623283941833705 82-83 19.977290778226443 30.03476079087333 30.907120154143925 19.080828276756296 84-85 19.794181918500396 29.730585220301574 30.887014222138333 19.588218639059697 86-87 18.478222991456466 31.98859313380997 28.71717767181447 20.816006202919088 88-89 28.711923028154008 18.796734865659882 36.10676705117218 16.384575055013933 90-91 30.59995193766078 15.917892190657785 17.754799339733765 35.72735653194767 92-93 22.408650260999256 18.21560341760368 36.84744094008471 22.528305381312354 94-95 15.622862738766127 7.2115214215109065 28.335046424103517 48.83056941561945 96-97 26.613676351168458 7.305247902192546 15.60357078408373 50.477504962555265 98-99 57.413380935976534 13.886192101719333 18.73832117169313 9.962105790611005 100-101 30.61973090255566 7.090186327176615 13.096336523829377 49.19374624643835 102-103 22.57859696994569 2.058597155781587 38.162950206819865 37.19985566745286 104-105 39.67010774590022 4.515837837212263 43.4148853680836 12.399169048803916 106-107 5.648922722103119 1.8371382490122805 6.848055668934689 85.66588335994992 108-109 17.947610655921498 11.661454085827373 22.677051965804583 47.713883292446546 110-111 40.18701944902183 2.8185174991153037 25.955263556110005 31.03919949575286 112-113 26.77284833565009 2.7585809633962772 39.74490932821171 30.723661372741923 114-115 28.21366101583041 3.96970330359402 24.862212736933586 42.95442294364199 116-117 19.644144134395 4.2167253555420405 20.757032384571932 55.382098125491034 118-119 20.17922336541653 4.263037255227348 16.318868237636906 59.238871141719216 120-121 25.08610877602791 4.1949666351740404 30.809087730522304 39.909836858275746 122-123 33.848298117649186 1.6674142285612796 34.568967852831655 29.915319800957885 124-125 38.58666134708658 2.10390363017658 20.47596651576726 38.833468506969574 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9.0 1 28.5 2 68.5 3 106.0 4 131.5 5 144.0 6 132.0 7 103.5 8 79.0 9 76.5 10 74.0 11 57.5 12 67.5 13 74.5 14 79.5 15 111.5 16 145.0 17 201.0 18 294.0 19 459.5 20 686.0 21 978.5 22 1437.0 23 1998.5 24 2751.5 25 3751.0 26 5021.5 27 6643.5 28 8639.5 29 11229.5 30 14570.0 31 18743.5 32 23818.0 33 29642.0 34 34795.5 35 38431.0 36 39930.5 37 39170.0 38 37499.0 39 35476.0 40 33417.5 41 31122.0 42 28614.5 43 26183.0 44 24074.5 45 22422.0 46 20961.0 47 19742.0 48 18372.5 49 16542.5 50 14785.0 51 13283.0 52 11777.0 53 10132.5 54 8357.0 55 6796.5 56 5610.5 57 4546.0 58 3514.0 59 2750.5 60 2116.5 61 1556.5 62 1156.5 63 866.0 64 642.5 65 430.5 66 306.5 67 225.5 68 169.5 69 131.0 70 106.5 71 81.0 72 56.0 73 42.5 74 34.5 75 31.5 76 23.5 77 15.0 78 13.5 79 10.5 80 6.0 81 5.5 82 4.0 83 3.0 84 2.0 85 3.0 86 3.5 87 1.5 88 1.5 89 1.0 90 1.5 91 3.5 92 2.5 93 0.5 94 1.0 95 1.0 96 0.5 97 2.5 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.807917643727812E-4 3 0.03905824615406953 4 0.04355938232795859 5 0.046753737032008885 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.711876465591719E-4 12-13 0.00246836499858432 14-15 1.451979410931953E-4 16-17 3.629948527329883E-4 18-19 0.007332496025206362 20-21 0.0032669536745968944 22-23 0.0011615835287455624 24-25 0.0 26-27 0.0 28-29 0.0011615835287455624 30-31 0.0 32-33 0.0014519794109319531 34-35 1.451979410931953E-4 36-37 1.451979410931953E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.903958821863906E-4 46-47 0.0 48-49 5.081927938261836E-4 50-51 2.1779691163979298E-4 52-53 0.0 54-55 0.0015971773520251484 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.903958821863906E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.081927938261836E-4 80-81 0.0 82-83 0.0011615835287455624 84-85 0.0 86-87 0.0010163855876523672 88-89 19.27059814291833 90-91 26.28678045345317 92-93 40.41860566417168 94-95 44.695483618042296 96-97 48.50467900365173 98-99 93.04552681442976 100-101 51.76749453692746 102-103 53.120594149974956 104-105 74.90776300792055 106-107 81.67972238153664 108-109 12.580820803960998 110-111 39.06978939038645 112-113 52.10740291702663 114-115 86.80136195668744 116-117 66.45629904967947 118-119 72.00119062311697 120-121 80.4820571644294 122-123 75.88327537515518 124-125 50.141132398742585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 688715.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.4417322802798 #Duplication Level Percentage of deduplicated Percentage of total 1 90.65275756227874 73.82917611856215 2 6.989320980556315 11.3844481623882 3 1.225324854015871 2.993777361514004 4 0.3956641844231942 1.2889430632275611 5 0.19510069763648238 0.7944669392303106 6 0.10987909281123115 0.5369246195959383 7 0.07179597134028731 0.40930317936888244 8 0.049994461140712765 0.3257308415775024 9 0.04344022284986248 0.3184062299580774 >10 0.2283953532340463 3.484418262901691 >50 0.022461480111771393 1.2517571649514834 >100 0.014260799644270905 2.2136745744818778 >500 0.0012478199667784388 0.7134048300718678 >1k 3.5651999050812536E-4 0.45556865217043574 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1686 0.24480372868312728 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1452 0.21082741046731956 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 938 0.1361956687454172 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 900 0.13067814698387575 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 832 0.12080468698953849 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.451979410931953E-4 0.0 5 0.0 0.0 0.0 1.451979410931953E-4 0.0 6 0.0 0.0 0.0 2.903958821863906E-4 0.0 7 0.0 0.0 0.0 2.903958821863906E-4 0.0 8 0.0 0.0 0.0 2.903958821863906E-4 0.0 9 0.0 0.0 0.0 2.903958821863906E-4 0.0 10-11 0.0 0.0 0.0 4.355938232795859E-4 0.0 12-13 0.0 0.0 0.0 4.355938232795859E-4 0.0 14-15 0.0 0.0 0.0 4.355938232795859E-4 0.0 16-17 0.0 0.0 0.0 4.355938232795859E-4 0.0 18-19 0.0 0.0 0.0 4.355938232795859E-4 0.0 20-21 0.0 0.0 0.0 4.355938232795859E-4 0.0 22-23 0.0 0.0 0.0 4.355938232795859E-4 0.0 24-25 0.0 0.0 0.0 4.355938232795859E-4 0.0 26-27 0.0 0.0 0.0 9.437866171057695E-4 0.0 28-29 0.0 0.0 0.0 0.00123418249929216 0.0 30-31 0.0 0.0 0.0 0.002323167057491125 0.0 32-33 0.0 0.0 0.0 0.005154526908808433 0.0 34-35 0.0 0.0 0.0 0.008421480583405326 0.0 36-37 0.0 0.0 0.0 0.01575397660861169 0.0 38-39 0.0 0.0 0.0 0.028531395424812877 0.0 40-41 0.0 0.0 0.0 0.04544695556217013 0.0 42-43 0.0 0.0 0.0 0.0649034796686583 0.0 44-45 0.0 0.0 0.0 0.08436000377514646 0.0 46-47 0.0 0.0 0.0 0.11107642493629441 0.0 48-49 0.0 0.0 0.0 0.13684905948033657 0.0 50-51 0.0 0.0 0.0 0.1640736734353107 0.0 52-53 0.0 0.0 0.0 0.19340365753613614 0.0 54-55 0.0 0.0 0.0 0.23006613766216794 0.0 56-57 0.0 0.0 0.0 0.27021336837443644 0.0 58-59 0.0 0.0 0.0 0.3189998765817501 0.0 60-61 0.0 0.0 0.0 0.3756270736080962 0.0 62-63 0.0 0.0 0.0 0.4348678335741199 0.0 64-65 0.0 0.0 0.0 0.49367299971686396 0.0 66-67 0.0 0.0 0.0 0.5655459805579957 0.0 68-69 0.0 0.0 0.0 0.6546975163892176 0.0 70-71 0.0 0.0 0.0 0.7574250597126533 0.0 72-73 0.0 0.0 0.0 0.850569538923938 0.0 74-75 0.0 0.0 0.0 0.9459645862221673 0.0 76-77 0.0 0.0 0.0 1.0525398749845727 0.0 78-79 0.0 0.0 0.0 1.0822328539381312 0.0 80-81 0.0 0.0 0.0 1.0825958487908642 0.0 82-83 0.0 0.0 0.0 1.0825958487908642 0.0 84-85 0.0 0.0 0.0 1.0825958487908642 0.0 86-87 0.0 0.0 0.0 1.0825958487908642 0.0 88-89 0.0 0.0 0.0 1.0825958487908642 0.0 90-91 0.0 0.0 0.0 1.0825958487908642 0.0 92-93 0.0 0.0 0.0 1.0825958487908642 0.0 94-95 0.0 0.0 0.0 1.0825958487908642 0.0 96-97 0.0 0.0 0.0 1.0825958487908642 0.0 98-99 0.0 0.0 0.0 1.0825958487908642 0.0 100-101 0.0 0.0 0.0 1.0825958487908642 0.0 102-103 0.0 0.0 0.0 1.0825958487908642 0.0 104-105 0.0 0.0 0.0 1.0825958487908642 0.0 106-107 0.0 0.0 0.0 1.0825958487908642 0.0 108-109 0.0 0.0 0.0 1.0825958487908642 0.0 110-111 0.0 0.0 0.0 1.0825958487908642 0.0 112-113 0.0 0.0 0.0 1.0825958487908642 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCG 15 3.378318E-7 3992.873 110-111 CAGCGCG 15 5.9723607E-7 3305.0251 108-109 CGGCGTT 10 9.086373E-4 2994.6548 110-111 CGGTAGT 10 9.086373E-4 2994.6548 110-111 CCGCTCG 25 6.87578E-10 2974.5227 108-109 CCGCTCC 165 0.0 2704.1116 108-109 CGTCGCG 30 1.4260877E-9 2478.7688 108-109 CCGCGCT 10 0.0013273134 2478.7688 108-109 CGTCGGC 15 0.0020442088 1996.4365 110-111 CGTCGCT 15 0.0020442088 1996.4365 110-111 CGACGCC 25 2.7641563E-6 1983.0151 108-109 GTTGTCG 35 4.28959E-6 1711.2313 110-111 CGGCGCG 15 0.0029860567 1652.5126 108-109 ACGCTCG 15 0.0029860567 1652.5126 108-109 CGTCGAC 15 0.0029860567 1652.5126 108-109 CCGCGTG 15 1.3561643E-4 1652.5126 108-109 CGCGTCT 15 0.0029860567 1652.5126 108-109 CCGCACC 45 7.219569E-9 1652.5126 108-109 CGTCGTG 20 0.0036337485 1497.3274 110-111 CGCGTGT 20 0.0036337485 1497.3274 110-111 >>END_MODULE