FastQCFastQC Report
Fri 27 May 2016
SRR939928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688715
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16500.23957660280377224No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15090.2191036931096317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13980.20298672164828702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.14418155550554293No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9510.13808324197962873No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA8690.12617701080998672No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA8280.1202238952251657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG8900.041.5300671
GGTATCA15300.040.523121
TACCTGG9200.039.516332
GTATCAA22950.037.4059561
GTACGAC700.002765715834.056233
ATCAACG25300.033.921623
TCAACGC25350.033.8547134
TAGGACC12550.033.7170224
TACATGG26250.033.1480642
ACCTGGG10700.032.8626673
CAACGCA26300.032.6318245
TATCAAC27150.031.8297162
GTACATG27000.031.7950631
AACGCAG27500.031.1535136
ACATGGG26050.030.4283583
TGTACGA1055.8464333E-428.380192
CATGGGG13500.028.2540554
ACGTCAA850.00714032628.0544661
CGGCCTG850.00715047628.0463075
ACGCAGA31000.027.636187
Produced by FastQC (version 0.11.5)