##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939928_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 688715 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.843565190245606 34.0 31.0 34.0 31.0 34.0 2 32.94658603341005 34.0 33.0 34.0 31.0 34.0 3 32.984784707752844 34.0 33.0 34.0 31.0 34.0 4 36.373909381964964 37.0 37.0 37.0 35.0 37.0 5 36.28332909839339 37.0 37.0 37.0 35.0 37.0 6 36.416527881634636 37.0 37.0 37.0 35.0 37.0 7 36.4149945913767 37.0 37.0 37.0 35.0 37.0 8 36.443399664592754 37.0 37.0 37.0 35.0 37.0 9 38.29013452589242 39.0 39.0 39.0 37.0 39.0 10-11 38.28030752923924 39.0 39.0 39.0 37.0 39.0 12-13 38.266947140689545 39.0 39.0 39.0 37.0 39.0 14-15 39.82656323733329 41.0 40.0 41.0 38.0 41.0 16-17 39.84551810255331 41.0 40.0 41.0 38.0 41.0 18-19 39.87898332401646 41.0 40.0 41.0 38.0 41.0 20-21 39.89488032059705 41.0 40.0 41.0 38.0 41.0 22-23 39.84485527395222 41.0 40.0 41.0 38.0 41.0 24-25 39.82917752626268 41.0 40.0 41.0 38.0 41.0 26-27 39.74838285793108 41.0 40.0 41.0 37.0 41.0 28-29 39.671137553269496 41.0 40.0 41.0 37.0 41.0 30-31 39.56204888814676 41.0 40.0 41.0 37.0 41.0 32-33 39.445379438519566 41.0 40.0 41.0 36.0 41.0 34-35 39.382931256034794 41.0 40.0 41.0 36.0 41.0 36-37 39.376698634413366 41.0 39.5 41.0 36.0 41.0 38-39 39.43320676912802 41.0 40.0 41.0 36.0 41.0 40-41 39.34203189998766 41.0 40.0 41.0 36.0 41.0 42-43 39.28170505942226 41.0 40.0 41.0 36.0 41.0 44-45 39.17716907574251 41.0 40.0 41.0 35.5 41.0 46-47 39.000279506036605 41.0 40.0 41.0 35.0 41.0 48-49 38.816270155289196 41.0 40.0 41.0 35.0 41.0 50-51 38.70063887094081 41.0 39.0 41.0 35.0 41.0 52-53 38.61264746665893 41.0 39.0 41.0 35.0 41.0 54-55 38.52968789702562 41.0 39.0 41.0 35.0 41.0 56-57 38.406221731775844 41.0 39.0 41.0 35.0 41.0 58-59 38.27542960440821 41.0 39.0 41.0 35.0 41.0 60-61 38.13196750470078 40.5 38.0 41.0 34.5 41.0 62-63 37.94685101965254 40.0 38.0 41.0 34.0 41.0 64-65 37.722657412717886 40.0 37.0 41.0 34.0 41.0 66-67 37.46996798385399 39.5 37.0 41.0 34.0 41.0 68-69 37.144932228860995 39.0 36.0 41.0 33.5 41.0 70-71 36.78337338376542 39.0 36.0 41.0 33.0 41.0 72-73 36.440679381166376 38.5 35.0 40.5 33.0 41.0 74-75 36.07274199051857 37.0 35.0 39.5 32.0 41.0 76-77 35.666209535148795 37.0 35.0 39.0 32.0 41.0 78-79 35.265881387801926 36.0 35.0 39.0 32.0 40.5 80-81 34.950089659728626 36.0 35.0 38.0 32.0 39.5 82-83 34.66876647089144 36.0 35.0 37.0 32.0 39.0 84-85 34.34292486732538 35.0 35.0 37.0 32.0 39.0 86-87 34.08017612510255 35.0 35.0 36.5 31.5 37.5 88-89 33.82441938973305 35.0 35.0 36.0 31.0 37.0 90-91 33.60316386313642 35.0 35.0 36.0 31.0 37.0 92-93 33.45762543287137 35.0 35.0 36.0 31.0 36.5 94-95 33.34435506704515 35.0 35.0 35.0 31.0 36.0 96-97 33.20085448988333 35.0 34.0 35.0 31.0 36.0 98-99 33.06231387438926 35.0 34.0 35.0 30.5 36.0 100-101 33.060800911843074 35.0 34.0 35.0 31.0 36.0 102-103 32.240934203552996 34.5 33.0 35.0 28.5 35.0 104-105 32.9007434134584 35.0 34.0 35.0 30.5 35.0 106-107 32.96299267476387 35.0 34.0 35.0 31.0 35.0 108-109 32.959984899414124 35.0 34.0 35.0 31.0 35.5 110-111 32.94418373347466 35.0 34.0 35.0 31.0 35.5 112-113 32.868893519089895 35.0 34.0 35.0 31.0 36.0 114-115 32.80626601714788 35.0 34.0 35.0 30.0 36.0 116-117 32.746144631669125 35.0 34.0 35.0 30.0 35.0 118-119 32.6691998867456 35.0 34.0 35.0 30.0 35.0 120-121 32.5624387446186 35.0 34.0 35.0 30.0 35.0 122-123 32.39315246509805 35.0 34.0 35.0 29.0 35.0 124-125 30.368293851593187 33.5 30.5 34.5 23.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0928501638363386 1101 2 0.1392126716364075 1101 3 0.14476835653582043 1101 4 0.09292732355632438 1101 5 0.09388782129389028 1101 6 0.09401028263539501 1101 7 0.14207358828640793 1101 8 0.16400809840187236 1101 9 0.17591674133594637 1101 10-11 0.11008076275420109 1101 12-13 0.07740012820218567 1101 14-15 0.21026945908453598 1101 16-17 0.2140360302443014 1101 18-19 0.2613377088741444 1101 20-21 0.18629077057543242 1101 22-23 0.1725543649053023 1101 24-25 0.1914483011429695 1101 26-27 0.27467920110101574 1101 28-29 0.27498285647337894 1101 30-31 0.2910039544877918 1101 32-33 0.36345028421209946 1101 34-35 0.3507272409883342 1101 36-37 0.2989259975078511 1101 38-39 0.15311400574113776 1101 40-41 0.24003924319416114 1101 42-43 0.1421889415870652 1101 44-45 0.10379642750092444 1101 46-47 0.18903719181208345 1101 48-49 0.1173399071935819 1101 50-51 0.2518322840452072 1101 52-53 0.27772208192330794 1101 54-55 0.3131935990286294 1101 56-57 0.28283052466493075 1101 58-59 0.27642516580215926 1101 60-61 0.31512257242317077 1101 62-63 0.2960024952152409 1101 64-65 0.2692622287425124 1101 66-67 0.2213614876998733 1101 68-69 0.262410150591883 1101 70-71 0.15002691898971676 1101 72-73 0.0795529419808716 1101 74-75 0.16605400808075643 1101 76-77 0.20431885395521476 1101 78-79 0.22831157927530654 1101 80-81 0.18023225889783845 1101 82-83 0.14187449165901 1101 84-85 0.18530543729757198 1101 86-87 0.2081168101227604 1101 88-89 0.15641302068484464 1101 90-91 0.1915797625591864 1101 92-93 0.2942265439471825 1101 94-95 0.29465321338742 1101 96-97 0.3472428830555927 1101 98-99 0.27795907153454635 1101 100-101 0.30453648958908985 1101 102-103 0.27957176926167904 1101 104-105 0.20770694631314512 1101 106-107 0.3565503222332893 1101 108-109 0.2916655385200002 1101 110-111 0.18840861459304392 1101 112-113 0.17677055464242386 1101 114-115 0.1950497058046068 1101 116-117 0.25550839042918483 1101 118-119 0.3378520599255168 1101 120-121 0.2730878748282919 1101 122-123 0.2132564199686371 1101 124-125 0.3777471991049417 1102 1 -0.014276755312671696 1102 2 0.03135091902104392 1102 3 0.06558012760247323 1102 4 0.0016760255293277737 1102 5 -0.09505341777479259 1102 6 -0.02225968123440225 1102 7 0.01832012330524435 1102 8 -0.08606270307079456 1102 9 -0.03413084583568349 1102 10-11 0.1018441914335213 1102 12-13 -0.004975239750926619 1102 14-15 -0.013607479373959563 1102 16-17 0.08559625869705201 1102 18-19 0.009026469891281863 1102 20-21 0.007629690788611754 1102 22-23 0.0732198987484054 1102 24-25 0.05439649591996698 1102 26-27 0.0926947430970202 1102 28-29 0.08515767174589683 1102 30-31 0.03340996016908093 1102 32-33 -0.014799610658435824 1102 34-35 0.01108131271666224 1102 36-37 0.0473689014838925 1102 38-39 0.03993409705358175 1102 40-41 0.08915004106239621 1102 42-43 0.07210996576255013 1102 44-45 0.06595518585764637 1102 46-47 -0.06283636533972015 1102 48-49 -0.051096796289847646 1102 50-51 -0.04889214375016593 1102 52-53 -9.863061202608492E-4 1102 54-55 -0.226099072804395 1102 56-57 -0.2283244619042506 1102 58-59 -0.2024813590899157 1102 60-61 -0.2717240158040468 1102 62-63 -0.17043170661615648 1102 64-65 -0.12299777572012971 1102 66-67 -0.10326487361935932 1102 68-69 -0.17639538565418178 1102 70-71 -0.26212293554543464 1102 72-73 -0.18791088490308994 1102 74-75 -0.15724536200068684 1102 76-77 -0.185290386280613 1102 78-79 -0.22278981272454956 1102 80-81 -0.17911042452088566 1102 82-83 -0.16992285736220936 1102 84-85 -0.20466156492516063 1102 86-87 -0.10723992202454724 1102 88-89 -0.16099319954634694 1102 90-91 -0.011757239577733003 1102 92-93 0.09728634759110122 1102 94-95 -0.04422140793197826 1102 96-97 -0.049594543890158604 1102 98-99 0.008251588891631911 1102 100-101 -0.014816771140544915 1102 102-103 0.021367916803363585 1102 104-105 0.11219790517963446 1102 106-107 0.015712032796869835 1102 108-109 0.05553350879196017 1102 110-111 0.10889319703599654 1102 112-113 0.1317241904053219 1102 114-115 0.07641325634827467 1102 116-117 0.05575544465882132 1102 118-119 0.008396716345281163 1102 120-121 0.019917363427111923 1102 122-123 -4.7606203062855457E-4 1102 124-125 -0.16522983118092682 1103 1 0.1218286165881537 1103 2 0.13681446207623083 1103 3 0.1278385253630745 1103 4 0.05711957391642386 1103 5 0.056597808564852414 1103 6 0.14462914841102759 1103 7 0.062233413094631373 1103 8 0.09969609458315887 1103 9 0.16433289716618305 1103 10-11 0.1933031357150483 1103 12-13 0.16400469893209646 1103 14-15 0.25287562513150164 1103 16-17 0.148096258697052 1103 18-19 0.1516842611469471 1103 20-21 0.17563072384646006 1103 22-23 0.11972832983344972 1103 24-25 0.1521704228727785 1103 26-27 0.21779055088160248 1103 28-29 0.16908112549156584 1103 30-31 0.14157278341620838 1103 32-33 0.22681095985609545 1103 34-35 0.21271504372172956 1103 36-37 0.15291575565611737 1103 38-39 0.12817723221753852 1103 40-41 0.1586356782258207 1103 42-43 0.17641131873509153 1103 44-45 0.14869978729726085 1103 46-47 0.1067600756093583 1103 48-49 0.242066693446219 1103 50-51 0.2008370951194678 1103 52-53 0.124213640560626 1103 54-55 0.11782588054137477 1103 56-57 0.15427351196938588 1103 58-59 0.17826710798552625 1103 60-61 0.18468760776300286 1103 62-63 0.19494585936624986 1103 64-65 0.1596810099370387 1103 66-67 0.2584761621044507 1103 68-69 0.10955752690247067 1103 70-71 0.13815782291897705 1103 72-73 0.2122657346650314 1103 74-75 0.07136450870045508 1103 76-77 0.0481473636527312 1103 78-79 0.028751442940603056 1103 80-81 -0.019257218709100243 1103 82-83 0.07629481792437787 1103 84-85 0.07055671020147258 1103 86-87 -7.933077883421902E-4 1103 88-89 -0.07337073220431023 1103 90-91 0.0029555036907353838 1103 92-93 -0.17250837381652673 1103 94-95 -0.10300573211219444 1103 96-97 -0.0566926510615815 1103 98-99 -0.14345795522992688 1103 100-101 -0.10259752960496371 1103 102-103 -0.16907713118384038 1103 104-105 -0.05895228810215514 1103 106-107 -0.13256509330283706 1103 108-109 -0.08649944908594165 1103 110-111 -0.054667353483864645 1103 112-113 -0.003681368112410155 1103 114-115 0.05243631686546735 1103 116-117 0.08404373444821545 1103 118-119 -0.06728310236972845 1103 120-121 0.09465993207547285 1103 122-123 0.13444629200829894 1103 124-125 0.03219252285800067 1104 1 0.0778310918356766 1104 2 0.08589023658916517 1104 3 0.11590743306029339 1104 4 0.10489384731150864 1104 5 0.10199265046839656 1104 6 0.09305616853239229 1104 7 0.07647207486403573 1104 8 -0.0289069874992407 1104 9 -0.0784050459829686 1104 10-11 0.026866693683743392 1104 12-13 0.009231862777504318 1104 14-15 -0.012998895788328468 1104 16-17 0.09859130820200335 1104 18-19 0.040614515059431255 1104 20-21 0.11334730800487591 1104 22-23 0.1805106278213131 1104 24-25 0.17356534462301454 1104 26-27 0.15436931964558198 1104 28-29 0.13175778630280632 1104 30-31 0.06536110982949594 1104 32-33 0.10011222143386789 1104 34-35 0.19032503254319266 1104 36-37 0.24202666271455797 1104 38-39 0.17267180264231996 1104 40-41 0.2595728788007108 1104 42-43 0.18628536057475742 1104 44-45 0.19109838199543105 1104 46-47 0.2560194975192971 1104 48-49 0.2985881722069976 1104 50-51 0.1657414787029836 1104 52-53 0.1930454834223312 1104 54-55 0.328409559877052 1104 56-57 0.2643142337835016 1104 58-59 0.15467640214075118 1104 60-61 0.14815774509931856 1104 62-63 0.17800438699320154 1104 64-65 0.202972889528219 1104 66-67 0.16676724322869774 1104 68-69 0.16807417493824772 1104 70-71 0.21276557603300006 1104 72-73 0.19459566120607263 1104 74-75 0.10747417813514204 1104 76-77 0.046266974000872096 1104 78-79 0.12329990988407502 1104 80-81 0.053072907448679985 1104 82-83 0.10612554293236087 1104 84-85 0.06640667826279412 1104 86-87 0.03327128818739311 1104 88-89 0.10953908095442699 1104 90-91 0.01613020825796241 1104 92-93 0.09977284624096683 1104 94-95 0.15535820912063514 1104 96-97 0.05466554120925338 1104 98-99 0.05664983780743427 1104 100-101 0.07526996480576997 1104 102-103 0.07051385412436417 1104 104-105 0.18794866207350935 1104 106-107 0.23362382397598935 1104 108-109 0.14878181089489573 1104 110-111 0.0600653597068046 1104 112-113 0.11520037893326673 1104 114-115 0.12802319006891594 1104 116-117 0.024922247702761524 1104 118-119 0.019158928930174568 1104 120-121 0.05531647151974539 1104 122-123 0.08316275639667481 1104 124-125 0.11432482883052941 1105 1 0.028580624620282435 1105 2 0.062197445623326075 1105 3 0.03802362058265629 1105 4 0.11061176850121512 1105 5 0.07128964040835228 1105 6 0.11426575937099415 1105 7 -9.345814642500727E-4 1105 8 0.07740936702777645 1105 9 -1.3950858586042614E-4 1105 10-11 0.10202673980884214 1105 12-13 0.0663762820387106 1105 14-15 0.06377851086799069 1105 16-17 0.0301243711468544 1105 18-19 0.12090995424190965 1105 20-21 0.13867038248100272 1105 22-23 0.16047802215111062 1105 24-25 0.14215455294144874 1105 26-27 0.1300383323299812 1105 28-29 0.14812026592426975 1105 30-31 0.053336774865883285 1105 32-33 0.10354774570918579 1105 34-35 0.13493416213085396 1105 36-37 0.09245212694772675 1105 38-39 0.1007206316216056 1105 40-41 0.08170497152806178 1105 42-43 0.08754982437054082 1105 44-45 0.05634650319309742 1105 46-47 0.19740086457165518 1105 48-49 0.24705033770065654 1105 50-51 0.19845053274092095 1105 52-53 0.21385446191054314 1105 54-55 0.11145361729483483 1105 56-57 0.2842693015794353 1105 58-59 0.16464001898436464 1105 60-61 0.20573955749742368 1105 62-63 0.2508671250742438 1105 64-65 0.25734383867132493 1105 66-67 0.2096417113984046 1105 68-69 0.18114427712918513 1105 70-71 0.3169516271682298 1105 72-73 0.20387734238623523 1105 74-75 0.1895529884258096 1105 76-77 0.16301813706898827 1105 78-79 0.23273269051153278 1105 80-81 0.12745260445451834 1105 82-83 0.24377117496544543 1105 84-85 0.2598825619957452 1105 86-87 0.1668004543570163 1105 88-89 0.1803639646476114 1105 90-91 0.36268474322953637 1105 92-93 0.16590171816429944 1105 94-95 0.3264783331812353 1105 96-97 0.20194674523327194 1105 98-99 0.19190770094314757 1105 100-101 0.3451146902421698 1105 102-103 0.28769147550096363 1105 104-105 0.31032927362864626 1105 106-107 0.24736592107726096 1105 108-109 0.29829173176650414 1105 110-111 0.3220350205283111 1105 112-113 0.33591492026691583 1105 114-115 0.30825799065740966 1105 116-117 0.1763099768371177 1105 118-119 0.23120115724939438 1105 120-121 0.10827285149508725 1105 122-123 0.19027039487136221 1105 124-125 -0.04752554295363254 1106 1 -0.0855246811809991 1106 2 -0.0029584251926237926 1106 3 -0.051058215977938914 1106 4 0.08545391107733735 1106 5 -0.027134122538683414 1106 6 0.027404249315253537 1106 7 -0.024744661746581187 1106 8 0.006169022526592016 1106 9 -0.07785093625579975 1106 10-11 0.00646018649567992 1106 12-13 -0.029927945540855205 1106 14-15 -0.15771804762592012 1106 16-17 -0.10360112248626763 1106 18-19 -0.09222873046783775 1106 20-21 0.004392241790206697 1106 22-23 -0.029791299558617368 1106 24-25 0.016123756623287022 1106 26-27 -0.10474756197469048 1106 28-29 -0.029198125001222763 1106 30-31 -0.10101681797298312 1106 32-33 -0.13207271868963488 1106 34-35 -0.04584959364881769 1106 36-37 -0.0203745434584377 1106 38-39 -0.15541253343435102 1106 40-41 -0.19315781618924888 1106 42-43 -0.20094946863076046 1106 44-45 -0.3182912330087362 1106 46-47 -0.16243226036260694 1106 48-49 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2216 30-31 -0.24112516379786797 2216 32-33 -0.32408975233494175 2216 34-35 -0.36008161526444127 2216 36-37 -0.2227388316920269 2216 38-39 -0.11679553694877853 2216 40-41 -0.1407791206849467 2216 42-43 -0.15099363518196185 2216 44-45 -0.17519003256030885 2216 46-47 -0.1479529532972208 2216 48-49 -0.16229809499115078 2216 50-51 -0.26841693713859627 2216 52-53 -0.27153677837527823 2216 54-55 -0.42341312239117457 2216 56-57 -0.4938203296728432 2216 58-59 -0.5192611701879102 2216 60-61 -0.4966723629114824 2216 62-63 -0.3948635247979766 2216 64-65 -0.48371944041905834 2216 66-67 -0.5307663494162824 2216 68-69 -0.5409910172952621 2216 70-71 -0.6067953086269 2216 72-73 -0.5107783583387473 2216 74-75 -0.5755968968295022 2216 76-77 -0.6582833024553452 2216 78-79 -0.5459893404695393 2216 80-81 -0.680475536681449 2216 82-83 -0.4509883827459191 2216 84-85 -0.3656341151022602 2216 86-87 -0.396164066062326 2216 88-89 -0.3918078394519071 2216 90-91 -0.4090417732258942 2216 92-93 -0.3872915744868237 2216 94-95 -0.35506113638534487 2216 96-97 -0.46838881779807906 2216 98-99 -0.44016930839290325 2216 100-101 -0.42298530715000027 2216 102-103 -0.29692458614823636 2216 104-105 -0.46376874180973715 2216 106-107 -0.5321775775927406 2216 108-109 -0.5040148973473535 2216 110-111 -0.5000944062697954 2216 112-113 -0.44623478244001546 2216 114-115 -0.5231292466033182 2216 116-117 -0.7074898327909978 2216 118-119 -0.6233550546110393 2216 120-121 -0.6127198856874116 2216 122-123 -0.7839397574261397 2216 124-125 -0.712492227875142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68.0 3 3.0 4 4.0 5 22.0 6 44.0 7 118.0 8 175.0 9 342.0 10 683.0 11 1124.0 12 1389.0 13 2364.0 14 3996.0 15 4568.0 16 1685.0 17 1097.0 18 954.0 19 874.0 20 871.0 21 864.0 22 979.0 23 1082.0 24 1280.0 25 1694.0 26 2165.0 27 2953.0 28 3669.0 29 4740.0 30 6248.0 31 7879.0 32 10399.0 33 13973.0 34 19946.0 35 32292.0 36 67787.0 37 236365.0 38 250758.0 39 3261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.37533800170199 20.570398747614746 15.241952582463703 23.812310668219563 2 12.901853451718054 20.166106444610616 45.91565451601896 21.016385587652366 3 19.090189701110038 25.797898985792383 29.50218885896198 25.609722454135603 4 11.179225078588386 17.886353571506355 39.89095634623901 31.04346500366625 5 11.490519790536945 38.48840015468399 35.273182971967884 14.747897082811178 6 28.67064025032125 37.82943597859783 20.131113740807155 13.368810030273771 7 27.1482398379591 32.01237086458114 22.487531126808623 18.351858170651138 8 23.45905055066319 40.15812055785049 20.655133110212496 15.72769578127382 9 25.340235075466627 18.06131709052366 22.5647764314702 34.03367140253951 10-11 23.442860980231302 26.783865604785724 30.724900720907776 19.048372694075198 12-13 26.271026476844554 24.474710148610093 29.971686401486824 19.28257697305852 14-15 20.069912808636374 29.9956440617672 26.731086153198348 23.203356976398073 16-17 18.895551861074612 30.746390016189572 31.27454752691607 19.083510595819753 18-19 20.25416899588364 27.864864276224566 32.99862787945667 18.88233884843513 20-21 20.73288842810802 28.405035095820157 30.833355113941625 20.028721362130202 22-23 19.47272781391999 30.590106640705255 31.142657806681868 18.794507738692882 24-25 20.330180118045927 28.40354863768032 32.97372643255919 18.29254481171457 26-27 18.2193650494036 30.377514646842307 32.214631596523965 19.188488707230132 28-29 18.397159928272217 30.246110510152967 30.18098923357267 21.17574032800215 30-31 19.63685994932592 28.469105508083896 31.883217295978746 20.010817246611442 32-33 18.516294838939185 29.702053824876764 32.85793107453736 18.92372026164669 34-35 18.82571165140878 30.12051429110735 32.52557298737504 18.528201070108828 36-37 18.149016646943945 29.529994264681324 32.045330797209296 20.27565829116543 38-39 19.85364047537806 30.03078196351176 30.83024182717089 19.285335733939295 40-41 21.739979527090306 27.50114343378611 32.433227096839765 18.325649942283817 42-43 18.94187000428334 30.30331849894369 32.335363684542955 18.419447812230022 44-45 18.440864508541267 30.611864123766725 31.163180706097588 19.78409066159442 46-47 20.54993720189048 28.532774805253265 30.785157866461454 20.132130126394806 48-49 19.93574991106626 29.271251533653253 30.348402459653123 20.444596095627364 50-51 18.70628634485963 31.00411636162999 30.27711027057636 20.012487022934014 52-53 18.714780424413583 29.854148668171888 31.37916264347372 20.051908263940817 54-55 19.162643473715544 29.042709974372567 31.46533762151253 20.329308930399367 56-57 19.693414547381717 29.593227968027414 29.07900945964586 21.63434802494501 58-59 19.68208910797645 30.388840086247576 28.736705313518655 21.19236549225732 60-61 19.324539178034456 27.992202870563293 32.166062885228285 20.51719506617396 62-63 20.059676353789303 28.09667278917985 31.474339893860304 20.36931096317054 64-65 20.5739674611414 29.440770129879557 29.53456799982576 20.45069440915328 66-67 20.246909098828976 31.189025939612176 28.731550786609844 19.832514174948997 68-69 21.092614506726296 28.3697175174056 31.304748698663452 19.232919277204648 70-71 21.93447216918464 28.272144500990976 30.26571223220055 19.527671097623838 72-73 20.583187530400817 30.085085993480615 29.593373165968508 19.73835331015006 74-75 19.74967874955533 30.42419578490377 31.138860050964478 18.68726541457642 76-77 20.246328307064605 28.67100324517398 31.72654871753919 19.356119730222225 78-79 19.63192321932875 28.662436566649486 30.903276391540768 20.802363822480995 80-81 20.108027268173338 29.63918311638341 30.524382364258074 19.72840725118518 82-83 19.712072482812193 29.401784482696037 31.734462005328762 19.151681029163008 84-85 19.51939481498152 29.10630667257138 31.72538713401044 19.648911378436654 86-87 18.236498406452597 31.186194579760855 29.540303318498946 21.037003695287602 88-89 19.701545632082937 29.118575898593758 30.734701581931567 20.445176887391735 90-91 19.78445052671318 29.408019398726598 31.651868361635245 19.15566171292498 92-93 18.735979323813186 30.660941027856225 31.41923727521544 19.183842373115148 94-95 18.78156263158659 30.637205790535933 30.799973864180856 19.781257713696622 96-97 20.48307355001706 29.736538335886397 30.066645854961777 19.713742259134765 98-99 20.312661082240332 28.773952702261212 31.748901192244528 19.164485023253924 100-101 19.671982378726696 29.133543290165857 31.7324487849075 19.46202554619994 102-103 18.810561461511824 30.441629042058977 30.785895109926294 19.96191438650291 104-105 19.315835479908348 29.460471559212074 30.872419390905105 20.351273569974474 106-107 19.584122904460077 28.4734958216259 30.626066374797617 21.316314899116406 108-109 18.88841537917601 29.731432873464247 30.302354908194374 21.077796839165366 110-111 18.596889398077764 29.909086552912324 30.953552971857633 20.54047107715228 112-113 19.356293164036405 28.67631256425597 30.527320039729787 21.440074231977835 114-115 19.1722172125732 29.269491527884867 29.09183049671033 22.4664607628316 116-117 19.492378784743774 29.302710531760813 28.18306888678348 23.021841796711932 118-119 19.439664998337477 28.31905307435753 30.056351484580663 22.184930442724326 120-121 19.144411814885128 27.869015510938972 31.022458736110757 21.964113938065143 122-123 20.02919964513211 28.88536053793558 29.862626632225314 21.222813184706997 124-125 20.536415657883463 29.243314587898084 28.777582724578593 21.44268702963986 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 92.0 1 55.0 2 58.5 3 89.5 4 49.5 5 20.0 6 62.5 7 206.5 8 338.5 9 338.0 10 243.0 11 162.5 12 177.5 13 228.0 14 301.0 15 371.5 16 445.5 17 569.0 18 696.0 19 847.0 20 1054.0 21 1306.5 22 1654.5 23 2060.5 24 2608.0 25 3472.5 26 4479.0 27 5563.5 28 6871.0 29 8520.5 30 10760.5 31 13977.5 32 18398.5 33 24572.5 34 32589.5 35 40314.0 36 45545.5 37 46509.5 38 42616.0 39 35898.5 40 30363.5 41 27277.0 42 24738.5 43 22499.5 44 21590.0 45 21242.0 46 20543.0 47 20132.5 48 19619.0 49 18360.0 50 17225.0 51 15850.5 52 13860.5 53 12123.0 54 10395.5 55 8677.0 56 7043.5 57 5568.5 58 4403.0 59 3468.0 60 2653.0 61 1963.5 62 1413.5 63 977.5 64 669.0 65 515.5 66 397.5 67 292.5 68 208.5 69 144.0 70 98.5 71 71.5 72 59.0 73 59.5 74 51.5 75 33.0 76 17.0 77 12.5 78 9.0 79 3.5 80 1.5 81 0.5 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 2.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015826575579158286 2 0.0 3 0.0 4 0.0 5 0.12501542728124115 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004210740291702664 22-23 7.259897054659765E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0014519794109319531 92-93 0.0 94-95 7.259897054659766E-4 96-97 0.0 98-99 0.0020327711753047343 100-101 2.903958821863906E-4 102-103 5.081927938261836E-4 104-105 0.003049156762957101 106-107 0.007259897054659765 108-109 6.533907349193788E-4 110-111 0.005517521761541421 112-113 0.009147470288871303 114-115 0.0063887094081005935 116-117 0.0018149742636649412 118-119 2.903958821863906E-4 120-121 0.0010889845581989646 122-123 0.0011615835287455624 124-125 0.0020327711753047343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 688715.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.79129588236178 #Duplication Level Percentage of deduplicated Percentage of total 1 90.17133647955326 71.94887789148036 2 7.299662703670981 11.648990932601043 3 1.266831427375652 3.03246363764416 4 0.4346390314392041 1.3872164623835475 5 0.2027741207766339 0.8089804934087077 6 0.13312092738081252 0.6373134782866084 7 0.08498624616253189 0.4746813899440071 8 0.05762659254773086 0.36784803973346375 9 0.04040130123198767 0.290130496257056 >10 0.2658697871918827 3.938093978497427 >50 0.024559540699587757 1.3611437534232922 >100 0.016190739355452764 2.573744447825847 >500 0.0014553473558296293 0.8690433702680509 >1k 5.457552584361109E-4 0.661471628246452 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1650 0.23957660280377224 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1509 0.2191036931096317 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1398 0.20298672164828702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 993 0.14418155550554293 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 951 0.13808324197962873 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 869 0.12617701080998672 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 828 0.1202238952251657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.451979410931953E-4 0.0 18-19 0.0 0.0 0.0 1.451979410931953E-4 0.0 20-21 0.0 0.0 0.0 1.451979410931953E-4 0.0 22-23 0.0 0.0 0.0 1.451979410931953E-4 0.0 24-25 0.0 0.0 0.0 1.451979410931953E-4 0.0 26-27 0.0 0.0 0.0 7.259897054659765E-4 0.0 28-29 0.0 0.0 0.0 0.0010889845581989648 0.0 30-31 0.0 0.0 0.0 0.0021779691163979296 0.0 32-33 0.0 0.0 0.0 0.005009328967715238 0.0 34-35 0.0 0.0 0.0 0.008276282642312132 0.0 36-37 0.0 0.0 0.0 0.015608778667518496 0.0 38-39 0.0 0.0 0.0 0.028240999542626488 0.0 40-41 0.0 0.0 0.0 0.04544695556217013 0.0 42-43 0.0 0.0 0.0 0.0648308806981117 0.0 44-45 0.0 0.0 0.0 0.08428740480459987 0.0 46-47 0.0 0.0 0.0 0.1108586280246546 0.0 48-49 0.0 0.0 0.0 0.13634086668651038 0.0 50-51 0.0 0.0 0.0 0.1633476837298447 0.0 52-53 0.0 0.0 0.0 0.19238727194848376 0.0 54-55 0.0 0.0 0.0 0.22904975207451558 0.0 56-57 0.0 0.0 0.0 0.2689791858751443 0.0 58-59 0.0 0.0 0.0 0.3174026992297249 0.0 60-61 0.0 0.0 0.0 0.3741024952266177 0.0 62-63 0.0 0.0 0.0 0.4325446665166288 0.0 64-65 0.0 0.0 0.0 0.4912046347182797 0.0 66-67 0.0 0.0 0.0 0.5619160320306658 0.0 68-69 0.0 0.0 0.0 0.6503415781564217 0.0 70-71 0.0 0.0 0.0 0.7516171420689255 0.0 72-73 0.0 0.0 0.0 0.8445438243685705 0.0 74-75 0.0 0.0 0.0 0.9391402829907871 0.0 76-77 0.0 0.0 0.0 1.0448443841066335 0.0 78-79 0.0 0.0 0.0 1.158969965805885 0.0 80-81 0.0 0.0 0.0 1.2852921745569648 0.0 82-83 0.0 0.0 0.0 1.4161881184524803 0.0 84-85 0.0 0.0 0.0 1.54977022425822 0.0 86-87 0.0 0.0 0.0 1.6874178724145692 0.0 88-89 0.0 0.0 0.0 1.833414402183777 0.0 90-91 0.0 0.0 0.0 1.9975606745896344 0.0 92-93 0.0 0.0 0.0 2.1828332474245515 0.0 94-95 0.0 0.0 0.0 2.361354115998635 0.0 96-97 0.0 0.0 0.0 2.539511989719986 0.0 98-99 0.0 0.0 0.0 2.7369811896067313 0.0 100-101 0.0 0.0 0.0 2.95935183639096 0.0 102-103 0.0 0.0 0.0 3.195516287579042 0.0 104-105 0.0 0.0 0.0 3.423985247889185 0.0 106-107 0.0 0.0 0.0 3.669224570395592 0.0 108-109 0.0 0.0 0.0 3.9319602448037285 0.0 110-111 0.0 0.0 0.0 4.210740291702663 0.0 112-113 0.0 0.0 0.0 4.528360787844028 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 890 0.0 41.530067 1 GGTATCA 1530 0.0 40.52312 1 TACCTGG 920 0.0 39.51633 2 GTATCAA 2295 0.0 37.405956 1 GTACGAC 70 0.0027657158 34.05623 3 ATCAACG 2530 0.0 33.92162 3 TCAACGC 2535 0.0 33.854713 4 TAGGACC 1255 0.0 33.717022 4 TACATGG 2625 0.0 33.148064 2 ACCTGGG 1070 0.0 32.862667 3 CAACGCA 2630 0.0 32.631824 5 TATCAAC 2715 0.0 31.829716 2 GTACATG 2700 0.0 31.795063 1 AACGCAG 2750 0.0 31.153513 6 ACATGGG 2605 0.0 30.428358 3 TGTACGA 105 5.8464333E-4 28.38019 2 CATGGGG 1350 0.0 28.254055 4 ACGTCAA 85 0.007140326 28.054466 1 CGGCCTG 85 0.007150476 28.046307 5 ACGCAGA 3100 0.0 27.63618 7 >>END_MODULE