FastQCFastQC Report
Fri 27 May 2016
SRR939927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232874
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4300.01637.186108-109
CCGCTCG1850.01630.8649108-109
CTCCGCC2800.01296.7479110-111
TTGTTCG1750.01293.0428108-109
CCGCACG401.09043685E-51257.125108-109
GGTCGTA250.0055734141210.298110-111
CGTCGGT501.2201297E-51210.298110-111
CCGCGCC5050.01194.891108-109
CGTCGCC1950.01160.4231108-109
CGTCGCG755.3158146E-81005.7108-109
CGCCGCC2700.0672.3878110-111
CGCGTCG451.5236656E-6672.38776110-111
CCGCGCG2107.2759576E-12598.6309108-109
AAGTTCG951.4584247E-4529.3158108-109
CCGCACC2401.6370905E-11523.80206108-109
CGCGTCT1251.9029426E-4484.11923110-111
CGTGTCT2555.6006684E-9474.62665110-111
CAGCGCG1152.5855296E-4437.26086108-109
GGTCGCC1253.3193835E-4402.28108-109
CCTCGCG1902.1815613E-6396.98688108-109
Produced by FastQC (version 0.11.5)