FastQCFastQC Report
Fri 27 May 2016
SRR939927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232874
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.2178649237472767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.13083251005374433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48150.034.2750851
GTACATG50400.030.1442661
GGTATCA34650.029.5746961
ATCAACG55200.029.138483
TACATGG49800.029.0682662
TCAACGC56600.028.312494
CAACGCA57300.027.9666125
TATCAAC58800.027.8610532
ACATGGG50500.027.4856973
AACGCAG58700.027.162826
ACGCAGA65100.024.4924357
CGCAGAG65400.024.3800858
GAGTACT31500.023.23125812-13
GTAGGAC17850.023.0278323
TAGGACC10350.023.0229954
AGAGTAC57300.022.68689210-11
GTACCTG15250.022.6596531
TACCTGG14650.022.3649162
CATGGGG25800.021.9354274
AGTACTT34000.021.78555512-13
Produced by FastQC (version 0.11.5)