FastQCFastQC Report
Fri 27 May 2016
SRR939926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358378
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17900.1317748078958876No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17790.13096501857362236No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA15800.1163151935617332No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14940.10998411340584137No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA14730.10843815197242594No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA14560.10718665938347059No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA14340.10556708073894011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG557.2759576E-121811.9463108-109
CCGCTCC5650.01543.3612108-109
CGTCGCT852.1827873E-111440.8619110-111
CCTCGCC4500.01273.3956108-109
CCGCGCC4450.01119.7421108-109
CTCCGCC3800.01047.4686110-111
GCTCGCG502.1897287E-5996.5704108-109
CCGCTCG2700.0830.4754108-109
CGTCGCC2200.0792.7265108-109
CGTGTCT1851.0659278E-9662.01764110-111
CCTCTCG3200.0622.8565108-109
CCGCGCG2057.2759576E-12607.6649108-109
CGTTGCT2557.2759576E-12600.35913110-111
CGGCGCG851.0746883E-4586.2179108-109
CTCCGCT1706.357586E-7540.32324110-111
GGTCGCC2602.910383E-11479.12042108-109
CTACGCC1701.4530087E-6439.66342108-109
CCTCGCG2359.900759E-9424.07254108-109
CGACGCC1203.0207512E-4415.23767108-109
GGTTGCG1503.1737992E-4408.2442110-111
Produced by FastQC (version 0.11.5)